Article
Agronomy
Cem Erdogan, A. Sibel Velioglu, M. Oktay Gurkan, Ian Denholm, Graham D. Moores
Summary: This study investigated resistance to commonly used pyrethroid and neonicotinoid insecticides in Trialeurodes vaporariorum in Turkey. The results showed resistance levels of up to 8.1-fold, 16-fold, 11.4-fold for neonicotinoids and 56.5-fold, 116.4-fold for pyrethroids. Biochemical analyses revealed no positive relationship between esterase or glutathione-S-transferase activity and resistance to these insecticides.
Article
Biochemistry & Molecular Biology
Warin Rangubpit, Eukote Suwan, Danai Sangthong, Kannika Wongpanit, Roger W. Stich, Prapasiri Pongprayoon, Sathaporn Jittapalapong
Summary: In this study, molecular dynamics simulations were conducted to investigate the binding of GTX and GSH to RmGST. It was found that GSH binds tighter and sits rigidly inside the G-site, while GTX occupies both active sites.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xingang Li, Yuanting Pang, Yiwang Zhong, Zhandong Cai, Qibin Ma, Ke Wen, Hai Nian
Summary: In this study, a tau-like glutathione transferase family gene named GmGSTU23 was identified in soybean, which showed a concentration-time-specific expression pattern in response to salt stress. Transgenic lines with GmGSTU23 displayed enhanced salt tolerance, root length, and fresh weight compared to the wild type. Changes in glutathione pools and associated enzyme activity were observed, indicating that GmGSTU23 enhances the activity of glutathione transferase to scavenge reactive oxygen species and glutathione, thereby conferring enhanced tolerance to salt stress in plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Yan Shen, Qiong Li, Fujin Fang, Chuanli Yang, Yu Dong, Xiaoqin Li, Zhizhi Luo, Xiaobing Shen
Summary: This study compares the resistance rates of Blattella germanica to beta-cypermethrin nanoemulsion and conventional emulsion. The results show that the resistance factor of beta-cypermethrin nanoemulsion increases at a slower rate compared to beta-cypermethrin emulsion.
Article
Agronomy
Yushi Jiang, Yuzhe Zhang, Ruijie Duan, Jiayi Fan, Peng Jiao, Hongji Sun, Shuyan Guan, Siyan Liu
Summary: ZmGST26 was found to be downregulated in maize under drought stress. Overexpression of ZmGST26 in Arabidopsis resulted in increased sensitivity to drought stress and ABA, as well as inhibited ABA-mediated stomatal closure. ZmGST26 overexpression decreased the resistance of Arabidopsis to drought stress and led to the accumulation of reactive oxygen species.
Article
Neurosciences
Bo Cheng, Xiaoliang Hong, Linfang Wang, Yuanyuan Cao, Dengli Qin, Han Zhou, Dianshuai Gao
Summary: Curzerene inhibits the progression of glioma by suppressing the expression of GSTA4 and matrix metalloproteinase 9, and inhibiting the activation of the mTOR pathway. It also induces apoptosis and reduces invasion and migration of glioma cells. In vivo studies have shown that Curzerene significantly inhibits tumor growth and prolongs survival time.
CNS NEUROSCIENCE & THERAPEUTICS
(2022)
Article
Biochemistry & Molecular Biology
N. R. Prasannakumar, N. Jyothi, S. Saroja, A. N. Lokesha
Summary: Phthorimaea absoluta is a destructive pest of tomato, causing complete yield loss if not controlled. Synthetic insecticides, while effective, have negative environmental impacts. Herbal insecticides, such as essential oils, offer a sustainable alternative. This study investigated the insecticidal properties of Ocimum basilicum and Mentha piperita essential oils as well as their constituents against P. absoluta. Both oils showed significant mortality effects, with M. piperita being the most effective. The study suggests that these essential oils could be used as an additional approach for managing P. absoluta.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Editorial Material
Biochemistry & Molecular Biology
Tiffany M. Russell, Des R. Richardson
Summary: GSTs are versatile proteins that play important roles in detoxification and cellular nitric oxide metabolism. They have the ability to convert organic nitrates into nitric oxide, thereby regulating vasodilation. GSTs are also considered suitable targets for pharmaceutical development, particularly in the field of anti-cancer drugs.
Article
Microbiology
Alvaro de Obeso Fernandez del Valle, Christian Quintus Scheckhuber, David Armando Chavaro-Perez, Erandi Ortega-Barragan, Sutherland K. Maciver
Summary: Some members of Acanthamoeba are facultative pathogens that can infect the cornea, causing Acanthamoeba keratitis. During encystation, there is an upregulation of glutathione S-transferase (GST) genes, which play a role in maintaining redox balance. Inhibiting GST with ethacrynic acid decreases cell viability by 70%. Targeting GST and associated processes could be a potential treatment strategy to reduce relapses of Acanthamoeba infection.
Article
Food Science & Technology
Yu Zhang, Ke-Xin Cao, Qin-Jian Niu, Jiang Deng, Ling Zhao, Mahmoud Mohamed Khalil, Niel Alexander Karrow, Kamil Kuca, Lv-Hui Sun
Summary: This study aimed to identify the key glutathione S-transferase (GST) isozymes involved in the detoxification of Aflatoxin B1 (AFB1) in ducks' primary hepatocytes. The researchers isolated and cloned the full-length cDNA encoding 10 GST isozymes from ducks' liver. The results showed that overexpression of GST and GST3 mitigated the toxic effects of AFB1 and increased the detoxified product of AFB1 in ducks' primary hepatocytes.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Plant Sciences
Aiqin Zhang, Rui Luo, Jiawen Li, Rongqing Miao, Hui An, Xiufeng Yan, Qiuying Pang
Summary: This study provides direct evidence that GSTU20 and GSTF11 are critically involved in aliphatic GSL biosynthesis, revealing their non-redundant but partially overlapping roles in GSL metabolism, with GSTU20 playing a more important role than GSTF11.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Lulu Han, Lin Zhou, Hongzhu Zou, Meng Yuan, Yan Wang
Summary: Anthocyanins, the important chromogenic substances responsible for the coloration of flowers, are transported and accumulated predominantly by PsGSTF3 gene. Silencing of PsGSTF3 gene leads to decreased anthocyanin accumulation and affects structural genes in the anthocyanin biosynthesis pathway. Heterologous overexpression of PsGSTF3 enhances anthocyanin accumulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Shumin Ma, Lele Yang, Qingqing Zuo, Qilai Huang
Summary: This study reports a transfection-positive cell sorting system using Glutathione S-transferase as a plasmid marker, which allows the fusion protein to be displayed on the cell surface and isolates positive cells using magnetic beads. The system works efficiently in both adherent and suspension cells, significantly enhancing gene alteration through increased knockdown efficiency and overexpression level.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yeong-Chin Jou, Shou-Chieh Wang, Yuan-Chang Dia, Shou-Tsung Wang, Min-Hua Yu, Hsin-Yi Yang, Lei-Chin Chen, Cheng-Huang Shen, Yi-Wen Liu
Summary: The study demonstrated that GSTM5 acts as a tumor suppressor in bladder cancer cells without significantly affecting chemoresistance to cisplatin and mitomycin C, and the cellular levels of GSH are key mechanisms underlying the anticancer effect of GSTM5. Low gene expression and high DNA methylation levels of GSTM5 may serve as tumor markers for bladder cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Simeng Chu, Yujie Lu, Wenjuan Liu, Xiaoyao Ma, Jiamin Peng, Xiaoying Wang, Min Jiang, Gang Bai
Summary: This study investigates the mechanism of TET-induced hepatotoxicity and reveals that TET covalently binds to GST, leading to liver injury, while UA competitively binds to GST to protect the liver from TET-induced damage.
Article
Chemistry, Inorganic & Nuclear
Murugan Arunthirumeni, Venkatesan Veerammal, Muthugounder Subramanian Shivakumar
Summary: In this study, selenium nanoparticles (SeNPs) were synthesized using Trichoderma fungus culture filtrate and tested on Spodoptera litura larvae. The results showed that the SeNPs were effective as larvicidal and antifeedant agent against S. litura larvae.
JOURNAL OF CLUSTER SCIENCE
(2022)
Article
Environmental Sciences
Dharman Kalaimurugan, Kandhasamy Lalitha, Kaliannan Durairaj, Palaniappan Sivasankar, Sungkwon Park, Kannan Nithya, Muthugoundar Subramanian Shivakumar, Wen-Chao Liu, Balasubramanian Balamuralikrishnan, Srinivasan Venkatesan
Summary: Borassus flabellifer leaf extract has been used to synthesize zinc oxide nanoparticles (ZnO-NPs) with antimicrobial and antioxidant properties, which can efficiently degrade environmental toxic dyes.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Kandhasamy Lalitha, Srinivasan Venkatesan, Balasubramanian Balamuralikrishnan, Muthugoundar Subramanian Shivakumar
Summary: This study found that high temperature has little impact on the control efficacy and reproductive ability of entomopathogenic nematodes, indicating their potential application in tropical and subtropical agroecosystems for controlling lepidopteran insect pests.
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2022)
Article
Chemistry, Inorganic & Nuclear
Balasubbiramaniyan Guru Bharathi, Kandhasamy Lalitha, Muthugounder Subramanian Shivakumar
Summary: By synthesizing and characterizing CuNPs using Xenorhabdus sp, this study showed their high insecticidal and antimicrobial activities, which can be potentially used in biopesticides. Various experiments demonstrated the effectiveness of CuNPs against pests and pathogens.
INORGANIC AND NANO-METAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Dharmalingam Kirubakaran, Kuppusamy Selvam, Palanisamy Prakash, Peraman Manimegalai, Muthugounder Subaramanian Shivakumar, Sengottayan SenthilNathan
Summary: In this study, silver nanoparticles (AgNPs) were synthesized by combining aqueous leaf extract of Strobilanthes cordifolia J.R.I.Wood with silver nitrate. The synthesized AgNPs were characterized using various techniques and found to have good crystalline structure and spherical shape. They exhibited antibacterial, antioxidant, antidiabetic, and anticancer activities, indicating their potential biomedical applications.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Kandhasamy Lalitha, Kannan Nithya, Balasubbiramaniyan Guru Bharathi, Srinivasan Venkatesan, Muthugounder Subramanian Shivakumar
Summary: Entomopathogenic nematodes (EPNs) are effective biopesticides used in insect control, but their low popularity is due to the reduced infectivity caused by periodic release of stored EPNs. This study focuses on storing EPNs for a longer period of time using encapsulating beads. The results show that Arabic gum-sodium alginate combination is a promising approach and semi-field trials proved the efficacy of storing encapsulated EPNs in mosquito larval control.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Parasitology
Murugan Arunthirumeni, Gunasekar Vinitha, Muthugounder Subramanian Shivakumar
Summary: In this study, bioactive compounds from Penicillium sp. were isolated and tested for insecticidal activity on Spodoptera litura and Culex quinquefasciatus larvae. Functional groups such as chloroalkanes, sulfonates, phosphines, amines, carboxylic acid, alkanes, and isocyanates were identified by FTIR. Ethyl acetate extract of Penicillium sp. showed significant larvicidal activity and high antifeedant activity against the larvae.
PARASITOLOGY INTERNATIONAL
(2023)
Article
Multidisciplinary Sciences
H. J. Akshitha, M. S. Shivakumar, R. Sivaranjani, S. J. Ankegowda, P. Mohammed Faisal, Honnappa Asangi, M. Balaji Rajkumar
Summary: Black pepper, known as black gold, has been traded like gold in the past. The market value of black pepper is determined by its color, lustre, and density. Sun-drying is a common drying method, but it affects the shiny black color. Blanching is one technique to retain the color but requires extra time and resources. An experiment was conducted to find an alternative method to retain the black color without affecting quality, and covering the berries with a 100-gauge polythene sheet for 3 or 5 hours and subsequent drying under direct sunlight proved to be effective.
NATIONAL ACADEMY SCIENCE LETTERS-INDIA
(2023)
Article
Parasitology
Govindaraju Ramkumar, Ranganathan Muthusamy, Mathiyazhagan Narayanan, Muthugoundar Subramanian Shivakumar, Eliningaya J. Kweka
Summary: Mosquitoes are important vectors for disease transmission, and their resistance to insecticides poses a challenge for disease control. This study found that overexpression of cytochrome P450 and esterase genes may be associated with permethrin resistance in Culex quinquefasciatus mosquitoes.
PARASITOLOGY RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
G. Vengateswari, M. S. Shivakumar
Summary: In this study, immune-related proteins were identified in the larval hemolymph of Spodoptera litura after Bacillus thuringiensis bacterial challenge. The high expression of immune defense protein was observed in the treated sample. Arylphorin, ecdysone receptor, and apolipophorin-III were identified as the top-score immune proteins through MALDI-TOF/MS analysis. The docking result revealed the interaction between bacterial cell membrane protein (lipopolysaccharide) and the isolated proteins.
RESEARCH JOURNAL OF BIOTECHNOLOGY
(2023)
Article
Entomology
P. Vivekanandhan, K. Swathy, Stefano Bedini, M. S. Shivakumar
Summary: Synthetic pesticides pose significant problems in both terrestrial and aquatic habitats. Green pesticides derived from fungi provide efficient alternatives to chemical pesticides. Secondary metabolites from Metarhizium anisopliae showed low toxicity against major disease mosquitos and non-target species, while also exhibiting minimal harm to earthworms. The primary components of the fungal crude extract were identified, and the functional groups potentially responsible for mosquitocidal activity were revealed.
INTERNATIONAL JOURNAL OF TROPICAL INSECT SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Govindaraju Ramkumar, Muthugoundar Subramanian Shivakumar, Mohammed Ali Alshehri, Chellasamy Panneerselvam, Samy Sayed
Summary: Mosquitoes are important vectors that transmit many communicable diseases. This study found that leaf extract of Cipadessa baccifera and synthesized ZnNPs have insecticidal potential against major mosquito vectors. These findings suggest that these natural and synthetic compounds could be used as an eco-friendly approach for mosquito control.
GREEN PROCESSING AND SYNTHESIS
(2022)
Article
Entomology
Govindaraj Vengateswari, Murugan Arunthirumeni, Muthugounder Subramaniam Shivaswamy, Muthugounder Subramanian Shivakumar
Summary: The composition of nutrients in food plants affects the growth and development of Spodoptera litura larvae. In this study, the effects of plant nutrients on larvae were evaluated using five different food plants. The results showed that different plants had different nutrient contents, and these nutrients significantly affected the growth and development of the larvae.
INTERNATIONAL JOURNAL OF TROPICAL INSECT SCIENCE
(2022)
Article
Entomology
P. Vivekanandhan, K. Swathy, M. S. Shivakumar
Summary: The study found that the aucubin molecule has stability in different temperatures and exhibits strong insecticidal activity against three mosquito species with low toxicity to non-target organisms. Docking results showed a high interaction between aucubin and proteins. Aucubin has several advantages, including high stability, low price, and low toxicity to non-target organisms, making it a potential alternative insecticide.
INTERNATIONAL JOURNAL OF TROPICAL INSECT SCIENCE
(2022)
Article
Entomology
P. Vivekanandhan, K. Swathy, M. S. Shivakumar
Summary: The study identified an effective compound in fraction-3 of the entomopathogenic fungus Metarhizium anisopliae extract, which showed significant larvicidal activity against Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus mosquitoes. This compound, characterized through various analyses, could potentially be developed into a highly effective mosquito control product for public health purposes.
INTERNATIONAL JOURNAL OF TROPICAL INSECT SCIENCE
(2022)
