Article
Neurosciences
Zhiping Mi, Hao Liu, Marie E. Rose, Xiecheng Ma, Daniel P. Reay, Jie Ma, Jeremy Henchir, C. Edward Dixon, Steven H. Graham
Summary: UCHL1 is a multifunctional protein expressed at high levels in neurons throughout the brain, with its hydrolase activity playing a crucial role in the recovery after neuronal injury and the pathogenesis of neurodegenerative diseases.
EXPERIMENTAL NEUROLOGY
(2021)
Article
Cell Biology
Zhiping Mi, Steven H. Graham
Summary: This review focuses on the potential role of UCHL1 in the pathogenesis of neurodegenerative diseases and brain injury and recovery. It discusses the normal physiological functions of UCHL1, the effects of posttranslational modification sites and splice variants on UCHL1 function, mouse models with UCHL1 mutations and deletions, and the hypothesized role and pathogenic mechanisms of UCHL1 in neurodegenerative diseases and brain injury.
AGEING RESEARCH REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Yuan Hu, Chenyang Qi, Jiaoyu Shi, Weiqiang Tan, Zhonghua Zhao, Yanyong Xu, Huijuan Wu, Zhigang Zhang
Summary: UCHL1 deficiency leads to severe kidney damage and proteinuria in podocytes, which is associated with ER stress, protein accumulation, and cell apoptosis. UCHL1 may be a potential target for preventing non-immune complex-mediated glomerulopathy.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Bingchuan Geng, Xiaoliang Wang, Ki Ho Park, Kyung Eun Lee, Jongsoo Kim, Peng Chen, Xinyu Zhou, Tao Tan, Chunlin Yang, Xunchang Zou, Paul M. Janssen, Lei Cao, Lei Ye, Xuejun Wang, Chuanxi Cai, Hua Zhu
Summary: In this study, it was found that UCHL1 plays a protective role in myocardial infarction (MI) by stabilizing HIF-1α and promoting HIF-1α signaling. The absence of UCHL1 makes cardiomyocytes more susceptible to hypoxia/re-oxygenation induced injury.
Article
Biochemistry & Molecular Biology
Pinduo Liu, Anping Wu, Hui Li, Jun Zhang, Junjun Ni, Zhenzhen Quan, Hong Qing
Summary: This study revealed the degradation pathways of Rab21 protein through the ubiquitin-proteasome pathway and the autophagy-lysosome pathway, and demonstrated increased ubiquitination of Rab21 protein in the AD model. Moreover, the study suggested the involvement of the autophagy-lysosome pathway in maintaining the protein level of Rab21.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Zhiping Mi, Hao Liu, Marie E. Rose, Jie Ma, Daniel P. Reay, Xiecheng Ma, Jeremy J. Henchir, C. Edward Dixon, Steven H. Graham
Summary: The study found that mutating cysteine to alanine at site 152 of UCHL1 can reduce protein ubiquitination, decrease activation of autophagy markers, improve abnormal axons and cell death, and enhance motor and cognitive function after traumatic brain injury. This research provides a new target for future therapeutic approaches in TBI.
Article
Cell Biology
Xiaoli Wu, Yanrong Zheng, Mengru Liu, Yue Li, Shijia Ma, Weidong Tang, Wenping Yan, Ming Cao, Wanqing Zheng, Lei Jiang, Jiaying Wu, Feng Han, Zhenghong Qin, Liang Fang, Weiwei Hu, Zhong Chen, Xiangnan Zhang
Summary: Mitophagy is essential for promoting neuronal survival in cerebral ischemia, and the deficiency of mitophagy receptor BNIP3L/NIX can lead to increased brain injury in ischemic conditions. The degradation of BNIP3L by proteasomes in ischemic brains contributes to mitophagy deficiency, and pharmacological intervention targeting this process, such as using the proteasome inhibitor carfilzomib, can rescue mitophagy and protect against ischemic brain injury.
Article
Biotechnology & Applied Microbiology
Na Li, Hang Du, Lejiao Mao, Ge Xu, Mengling Zhang, Yinzhen Fan, Xiaomei Dong, Lijun Zheng, Bin Wang, Xia Qin, Xuejun Jiang, Chengzhi Chen, Zhen Zou, Jun Zhang
Summary: This study reveals that CuONPs exposure activates the NRF2 pathway in vascular endothelial cells and autophagy promotes NRF2 activation. CuONPs disrupt the ubiquitin-proteasome pathway, while autophagy deficiency reciprocally promotes proteasome activity. This research provides a novel regulatory mechanism for discovering therapeutic strategies against NPs-induced vascular injury and disease.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Cell Biology
Yusuke Nishimura, Jitpisute Chunthorng-Orn, Samuel Lord, Ibrahim Musa, Peter Dawson, Lars Holm, Yu-Chiang Lai
Summary: In this study, the researchers showed that Atrogin-1 and MuRF1 protein contents are regulated by different mechanisms, specifically the downstream of Akt, and that Atrogin-1 protein content can be regulated by the rapamycin-sensitive mTOR-S6K1-dependent signaling pathway.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Microbiology
Priyadharshini Ramachandran, J. Beslin Joshi, Julie A. Maupin-Furlow, Sivakumar Uthandi
Summary: Plant pathogenic Gram-negative bacteria evade the host plant immune system by secreting Type III and Type IV effector proteins, which mimic components of the ubiquitin-265 proteasome system to control plant cellular activities and establish pathogenicity.
MICROBIOLOGICAL RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Deepa Kumari, Jeffrey L. Brodsky
Summary: Protein quality control processes are crucial for cellular and organism health, particularly for polypeptides entering the endoplasmic reticulum (ER). ER-associated degradation (ERAD) is a multi-step pathway that targets inefficiently matured proteins for degradation, providing a mechanism to regulate protein levels and activities in the ER.
Review
Environmental Sciences
Amit Gupta, Tapan Behl, Lotfi Aleya, Md. Habibur Rahman, Harlokesh Narayan Yadav, Giridhari Pal, Ishnoor Kaur, Sandeep Arora
Summary: Type 2 diabetes is a common metabolic disorder, and therapies targeting UPP pathway may have promising results in treating diabetes and associated complications. The role of ubiquitin molecules extends beyond neurodegenerative disorders to play a critical role in diabetes progression.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Oncology
Srirupa Bhattacharyya, Janet L. Oblinger, Roberta L. Beauchamp, Zhenzhen Yin, Serkan Erdin, Priya Koundinya, Anna D. Ware, Marc Ferrer, Justin T. Jordan, Scott R. Plotkin, Lei Xu, Long-Sheng Chang, Vijaya Ramesh
Summary: This study identifies a class of compounds targeting the ubiquitin-proteasome pathway (UPP) that have potent efficacy in treating NF2-associated tumors. These compounds reduce cell viability and induce apoptosis by downregulating c-KIT and PDGFR alpha expression and upregulating genes associated with endoplasmic reticulum stress-mediated activation of the unfolded protein response (UPR). In vivo models further demonstrate the therapeutic potential of these drugs.
Article
Biochemistry & Molecular Biology
Abramo J. Manfredonia, Daniel A. Kraut
Summary: The ubiquitin-proteasome system is responsible for protein degradation in eukaryotic cells. The study showed that degradation of ubiquitin-independent degrons (UbIDs) is slower and relies on loosely folded substrates. Furthermore, UbID degradation is ATP-independent.
Article
Biochemistry & Molecular Biology
Wenyan Yang, Shiqun Wang, Shengqiang Tong, Wei-Dong Zhang, Jiang-Jiang Qin
Summary: This article provides an overview of the ubiquitin-proteasome system (UPS) and its role in pancreatic cancer. Studies indicate that mutations or aberrant expression of UPS members can lead to rewriting of the ubiquitination code, affecting tumor growth, metastasis, immune evasion, and drug resistance. The article also reviews current UPS modulators and analyzes their potential as cancer therapies.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Hao Liu, Jie Chen, Wenjin Li, Marie E. Rose, Sunita N. Shinde, Manimalha Balasubramani, Guy T. Uechi, Buelent Mutus, Steven H. Graham, Robert W. Hickey
Article
Cell Biology
H. Liu, W. Li, M. E. Rose, R. W. Hickey, J. Chen, G. T. Uechi, M. Balasubramani, B. W. Day, K. V. Patel, S. H. Graham
CELL DEATH & DISEASE
(2015)
Article
Biochemistry & Molecular Biology
Hao Liu, Marie E. Rose, Sherman Culver, Xiecheng Ma, C. Edward Dixon, Steven H. Graham
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2016)
Article
Cell Biology
Steven H. Graham
NEURAL REGENERATION RESEARCH
(2016)
Article
Multidisciplinary Sciences
Hao Liu, Marie E. Rose, Xiecheng Ma, Sherman Culver, C. Edward Dixon, Steven H. Graham
Review
Neurosciences
Milos D. Ikonomovic, Eric E. Abrahamson, Shaun W. Carlson, Steven H. Graham, C. Edward Dixon
Article
Biochemical Research Methods
Jafar Sadik B. Shaik, Tricia M. Miller, Steven H. Graham, Mioara D. Manole, Samuel M. Poloyac
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2014)
Article
Critical Care Medicine
Patrick M. Kochanek, Travis C. Jackson, Ruchira M. Jha, Robert S. B. Clark, David O. Okonkwo, Hulya Bayir, Samuel M. Poloyac, Amy K. Wagner, Philip E. Empey, Yvette P. Conley, Michael J. Bell, Anthony E. Kline, Corina O. Bondi, Dennis W. Simon, Shaun W. Carlson, Ava M. Puccio, Christopher M. Horvat, Alicia K. Au, Jonathan Elmer, Amery Treble-Barna, Milos D. Ikonomovic, Lori A. Shutter, D. Lansing Taylor, Andrew M. Stern, Steven H. Graham, Valerian E. Kagan, Edwin K. Jackson, Stephen R. Wisniewski, C. Edward Dixon
JOURNAL OF NEUROTRAUMA
(2020)
Article
Clinical Neurology
Fenghua Chen, Zhongfang Weng, Qinghai Xia, Catherine Cao, Rehana K. Leak, Lihong Han, Jian Xiao, Steven H. Graham, Guodong Cao
TRANSLATIONAL STROKE RESEARCH
(2019)
Article
Neurosciences
Zhiping Mi, Hao Liu, Marie E. Rose, Xiecheng Ma, Daniel P. Reay, Jie Ma, Jeremy Henchir, C. Edward Dixon, Steven H. Graham
Summary: UCHL1 is a multifunctional protein expressed at high levels in neurons throughout the brain, with its hydrolase activity playing a crucial role in the recovery after neuronal injury and the pathogenesis of neurodegenerative diseases.
EXPERIMENTAL NEUROLOGY
(2021)
Article
Neurosciences
Zhiping Mi, Hao Liu, Marie E. Rose, Jie Ma, Daniel P. Reay, Xiecheng Ma, Jeremy J. Henchir, C. Edward Dixon, Steven H. Graham
Summary: The study found that mutating cysteine to alanine at site 152 of UCHL1 can reduce protein ubiquitination, decrease activation of autophagy markers, improve abnormal axons and cell death, and enhance motor and cognitive function after traumatic brain injury. This research provides a new target for future therapeutic approaches in TBI.
Article
Cell Biology
Zhiping Mi, Steven H. Graham
Summary: This review focuses on the potential role of UCHL1 in the pathogenesis of neurodegenerative diseases and brain injury and recovery. It discusses the normal physiological functions of UCHL1, the effects of posttranslational modification sites and splice variants on UCHL1 function, mouse models with UCHL1 mutations and deletions, and the hypothesized role and pathogenic mechanisms of UCHL1 in neurodegenerative diseases and brain injury.
AGEING RESEARCH REVIEWS
(2023)
Meeting Abstract
Endocrinology & Metabolism
Z. Mi, N. Povysheva, M. Rose, J. Ma, D. Zeh, N. Harikumar, I. Bhuiyan, S. Graham
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2022)
Review
Cell Biology
Steven H. Graham, Hao Liu
AGEING RESEARCH REVIEWS
(2017)