Article
Oncology
Yuna Shin, Wonhee Jung, Mi-Yeon Kim, Dongjo Shin, Geun Hee Kim, Chun Ho Kim, Sun-Hoo Park, Eung-Ho Cho, Dong Wook Choi, Chul Ju Han, Kee Ho Lee, Sang-Bum Kim, Hyun Jin Shin
Summary: GPCRs are critical for drug development and abnormal activation of GPCRs is implicated in cancer. We discovered that NPFFR2 is aberrantly expressed in liver cancer and promotes malignancy through RhoA/YAP signaling.
Article
Biotechnology & Applied Microbiology
Zhuo Li, Zhiwen Lian, Jianchao Ma, Li Zhang, Xingji Lian, Shuangxin Liu, Jianteng Xie, Zhonglin Feng, Ting Lin, Hong Zhang, Xinling Liang
Summary: The axis formed by ITG beta 3, RhoA, and YAP plays a crucial role in podocyte injury induced by high glucose exposure. ITG beta 3 overexpression promotes podocyte injury by inhibiting the RhoA-YAP axis, while ITG beta 3 inhibition mitigates the ability of wound closure and apoptosis in podocytes under high glucose conditions.
Article
Biochemistry & Molecular Biology
Megha Amar, Akula Bala Pramod, Nam-Kyung Yu, Victor Munive Herrera, Lily R. Qiu, Patricia Moran-Losada, Pan Zhang, Cleber A. Trujillo, Jacob Ellegood, Jorge Urresti, Kevin Chau, Jolene Diedrich, Jiaye Chen, Jessica Gutierrez, Jonathan Sebat, Dhakshin Ramanathan, Jason P. Lerch, John R. Yates, Alysson R. Muotri, Lilia M. Iakoucheva
Summary: Cul3 mutant mice exhibit deficits in social and cognitive behaviors, hyperactive behavior, reduced cortical volume, and changes in brain regions, with neuronal cytoskeleton defects identified as key drivers of Cul3 functional impact. Specifically, dendritic growth, filamentous actin puncta, and spontaneous network activity are reduced in Cul3 mutant mice, with Rho signaling inhibition rescuing these phenotypes. The study highlights defects in neuronal cytoskeleton and Rho signaling as primary targets of Cul3 mutation during brain development.
MOLECULAR PSYCHIATRY
(2021)
Article
Engineering, Biomedical
Yaru Guo, Feng Mei, Ying Huang, Siqin Ma, Yan Wei, Xuehui Zhang, Mingming Xu, Ying He, Boon Chin Heng, Lili Chen, Xuliang Deng
Summary: This study demonstrates that matrix stiffness promotes sprouting of endothelial cells and regulates the formation of tip cells through a specific signaling axis. The findings have significant implications for biomaterial design and treatment of pathological conditions.
BIOACTIVE MATERIALS
(2022)
Article
Oncology
Patricia Dias Carvalho, Flavia Martins, Susana Mendonca, Andreia Ribeiro, Ana Luisa Machado, Joana Carvalho, Maria Jose Oliveira, Sergia Velho
Summary: This study reveals the role of mutant KRAS in modulating cell fate through autonomous and nonautonomous signaling mechanisms in colorectal cancer cells. Mutant KRAS autonomously controls processes such as proliferation and cell-cell aggregation, while cancer cell invasion depends on the cooperation between fibroblast-derived HGF and mutant KRAS regulation of C-MET expression.
INTERNATIONAL JOURNAL OF CANCER
(2022)
Article
Multidisciplinary Sciences
Hanna Lucie Sladitschek-Martens, Alberto Guarnieri, Giulia Brumana, Francesca Zanconato, Giusy Battilana, Romy Lucon Xiccato, Tito Panciera, Mattia Forcato, Silvio Bicciato, Vincenza Guzzardo, Matteo Fassan, Lorenzo Ulliana, Alessandro Gandin, Claudio Tripodo, Marco Foiani, Giovanna Brusatin, Michelangelo Cordenonsi, Stefano Piccolo
Summary: Ageing is closely related to the induction of cell senescence, with YAP/TAZ mechanotransduction playing a key role in the process. Declining YAP/TAZ activity during ageing leads to accelerated ageing and tissue degeneration, as it unleashes cGAS-STING signalling and promotes senescence. Sustaining YAP/TAZ mechanosignalling or inhibiting STING may be potential strategies to limit senescence-associated inflammation and improve healthy ageing.
Article
Biochemistry & Molecular Biology
Fangbiao Zhan, Tao He, Zhiyu Chen, Qiang Zuo, Yang Wang, Qiaochu Li, Shenxi Zhong, Yunsheng Ou
Summary: This study found that YAP expression levels are higher in osteosarcoma patients and are associated with a poor prognosis. MPPa-PDT induced apoptosis in OS cells and YAP knockdown enhanced this apoptosis. Both active and total RhoA protein levels increased in OS cells following MPPa-PDT treatment. Targeting the RhoA/ROCK2/LIMK2/YAP pathway can improve the efficacy of MPPa-PDT treatment for OS.
CELL AND BIOSCIENCE
(2021)
Article
Medicine, Research & Experimental
Wan-Hsin Lin, Ryan W. Feathers, Lisa M. Cooper, Laura J. Lewis-Tuffin, Jiaxiang Chen, Jann N. Sarkaria, Panos Z. Anastasiadis
Summary: The Rho family guanine nucleotide exchange factor Syx promotes GBM cell growth by regulating cell cycle progression, DNA damage, and therapy resistance. Depletion of Syx leads to prolonged mitosis, increased DNA damage, cell cycle arrest, and cell apoptosis. Targeting the Syx signaling pathway may provide a potential treatment option for GBM.
Article
Biochemistry & Molecular Biology
Cheng Yan, Huijie Yang, Peng Su, Xin Li, Zhongbo Li, Dehai Wang, Yifeng Zang, Tianshi Wang, Ziping Liu, Zhuocong Bao, Shuxiao Dong, Ting Zhuang, Jian Zhu, Yinlu Ding
Summary: Gastric cancer is a deadly disease and recent studies have shown that abnormality in the Hippo/YAP axis plays a critical role in its development. Inhibiting the deubiquitination of YAP protein could be a potential new therapeutic target for gastric cancer.
Article
Cell Biology
Shan Wang, Emelie Englund, Pontus Kjellman, Zhen Li, Johannes Kumra Ahnlide, Carmen Rodriguez-Cupello, Mattia Saggioro, Ryu Kanzaki, Kristian Pietras, David Lindgren, Hakan Axelson, Christelle N. Prinz, Vinay Swaminathan, Chris D. Madsen
Summary: Wang et al. have identified CCM3 as a negative regulator of YAP/TAZ activation and mechanotransduction in focal adhesions, with crucial roles in controlling mesenchymal/stromal stem cell differentiation and metastasis. CCM3 functions as a gatekeeper in focal adhesions, regulating mechanotransduction and YAP/TAZ signaling by competing with focal adhesion kinase for binding to paxillin. Loss of CCM3 in cancer-associated fibroblasts leads to exacerbated tissue remodelling and metastasis dissemination in mouse models of breast cancer.
NATURE CELL BIOLOGY
(2021)
Article
Cell Biology
Hualin Chen, Wenjie Yang, Yingjie Li, Zhigang Ji
Summary: PLAGL2 is overexpressed in bladder cancer tissues and is associated with decreased survival. Mechanistically, overexpressed PLAGL2 activates the RACGAP1/RhoA GTPase/YAP1 signaling pathway, promoting proliferation and metastasis of bladder cancer cells. Targeting the core nodes of this signaling pathway may have therapeutic potential for bladder cancer.
CELL DEATH & DISEASE
(2023)
Article
Cell Biology
Manuel Rogg, Jasmin I. Maier, Martin Helmstaedter, Alena Sammarco, Felix Kliewe, Oliver Kretz, Lisa Weisser, Clara Van Wymersch, Karla Findeisen, Anna L. Koessinger, Olga Tsoy, Jan Baumbach, Markus Grabbert, Martin Werner, Tobias B. Huber, Nicole Endlich, Oliver Schilling, Christoph Schell
Summary: Glomerular disease due to podocyte malfunction is a major factor in chronic kidney disease. Loss of function in the EPB41L5 gene affects protein uptake and slit diaphragm formation. Transcriptomic and proteomic analysis of human EPB41L5 knockout podocytes reveals impaired mechanotransduction through the YAP/TAZ signaling pathway. Inhibition of the YAP/TAZ-TEAD transcription factor complex further identifies ARHGAP29 as a podocyte RhoGAP that is dependent on EPB41L5 and YAP/TAZ.
Article
Biochemistry & Molecular Biology
Bi-Oh Park, Seong Heon Kim, Jong Hwan Kim, Seon-Young Kim, Byoung Chul Park, Sang-Bae Han, Sung Goo Park, Jeong-Hoon Kim, Sunhong Kim
Summary: GPR43, also known as FFAR2 or FFA2, is a G-protein-coupled receptor primarily expressed in immune cells, enteroendocrine cells, and adipocytes. It recognizes short-chain fatty acids and may play a role in innate immunity and host energy homeostasis. Activation of GPR43 can affect cAMP levels and Ca2+ flux through coupling to G alpha and G alpha(q) families for signal transduction.
MOLECULES AND CELLS
(2021)
Article
Cell Biology
Zhong-Ming Huang, Hai Wang, Zhi-Gang Ji
Summary: miR-217 expression was significantly higher in T24 and 5367 cell lines. Exosomal miR-217 mimic enhanced proliferation and migration, while inhibiting apoptosis of the cancer cells. Conversely, exosomal miR-217 inhibitor suppressed proliferation and migration but stimulated apoptosis. Additionally, miR-217 may act as an oncogene in bladder cancer cells through the Hippo-YAP signaling pathway.
INFLAMMATION RESEARCH
(2021)
Article
Medicine, Research & Experimental
Jian Song, Nan Jiang, Xueqi Gan, Wei Zhi, Zhuoli Zhu
Summary: The study demonstrated that the local injection of argatroban can rescue bone loss in periodontal disease, while inducing osteogenic differentiation through activating the canonical Wnt signaling pathway. Therefore, argatroban has potential applications in promoting alveolar bone regeneration and remodeling based on BMSCs.