Article
Materials Science, Multidisciplinary
Ryoma Ogawa, Amelia Hallas-Potts, Hancong Wu, Jiabin Jia, Pierre O. Bagnaninchi
Summary: This study demonstrates a method combining 3D cell culture and impedance imaging technology to simultaneously perform 3D impedance imaging and viability measurements. Utilizing low-resistance 3D printed scaffolds, real-time monitoring of large-scale 3D cell cultures is achieved, paving the way for quantitative, non-invasive evaluation of tissue engineering products.
ADVANCED ENGINEERING MATERIALS
(2021)
Review
Polymer Science
Mohammad Javad Javid-Naderi, Javad Behravan, Negar Karimi-Hajishohreh, Shirin Toosi
Summary: Damage or loss of bone tissue is a major issue affecting human well-being, and tissue engineering is used to recover and regenerate bone fractures and damage. Polymer biomaterials are the most commonly required materials for synthetic bone scaffolds due to their suitable mechanical properties and degradation rates. Synthetic polymers offer advantages in bone replacement because they can be designed and changed easily, and their biocompatibility has been widely accepted in tissue engineering. This article updates on materials and application usage of scaffolds in bone tissue engineering for hard tissue regeneration.
POLYMERS FOR ADVANCED TECHNOLOGIES
(2023)
Article
Orthopedics
Spencer E. Szczesny, David T. Corr
Summary: A breakout session was held at the 2022 ORS Tendon Section Meeting to establish guidelines for studying tendon cell and tissue cultures. This paper summarizes the outcomes of the discussion and provides recommendations for future studies.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Hae Sang Park, Ji Seung Lee, Chang-Beom Kim, Kwang-Ho Lee, In-Sun Hong, Harry Jung, Hanna Lee, Young Jin Lee, Olatunji Ajiteru, Md Tipu Sultan, Ok Joo Lee, Soon Hee Kim, Chan Hum Park
Summary: In this study, we successfully created a three-dimensional bioprinted artificial larynx using rabbit's chondrocyte-laden gelatin methacryloyl (GelMA)/glycidyl-methacrylated hyaluronic acid (GMHA) hybrid bioink. We incorporated a polycaprolactone (PCL) outer framework with pores to provide structural strength and a suitable microenvironment for the printed cells. Our novel fluidics supply (FS) system improved cell viability during the printing process, leading to the generation of a large-scale cell-laden artificial laryngeal framework. Animal studies confirmed the functionality of the transplanted 3D bio-larynx, offering potential for clinical applications in laryngectomy patients.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Review
Chemistry, Multidisciplinary
Oystein Arlov, Dominic Rutsche, Maryam Asadi Korayem, Ece Ozturk, Marcy Zenobi-Wong
Summary: Sulfated polysaccharides play central roles in biological functions, and engineered sulfated polysaccharides exhibit novel and augmented biological properties through facilitating interactions with other macromolecules.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Nanoscience & Nanotechnology
Xuelin Wang, Connie Wen, Brandon Davis, Peng Shi, Lidya Abune, Kyungsene Lee, Cheng Dong, Yong Wang
Summary: This study systematically evaluated and compared click conjugation and lipid insertion methods for engineering the cell surface with synthetic DNA. Results showed that lipid insertion is more efficient in displaying DNA on the cell surface, while physically inserted DNA has a shorter half-life. These findings can serve as a guideline for choosing an appropriate method for engineering the cell surface with synthetic DNA or other biomolecules.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Applied
Tomasz Machalowski, Joanna Idaszek, Adrian Chlanda, Marcin Heljak, Adam Piasecki, Wojciech Swieszkowski, Teofil Jesionowski
Summary: The study developed chitin-based biocomposites through biomimetic ex vivo deposition of calcium carbonate particles in the hemolymph from the cultivated mollusk Cornu aspersum and chitinous matrix from the marine demosponge Aplysina fistularis. The results showed that the biofunctionalized scaffold had about 4 times higher mechanical resistance and exhibited significant topographical changes, indicating its potential for modifying cell delivery systems in future biomedical applications.
CARBOHYDRATE POLYMERS
(2022)
Review
Biochemistry & Molecular Biology
Isadora C. Carvalho, Herman S. Mansur, Alice G. Leonel, Alexandra A. P. Mansur, Zelia I. P. Lobato
Summary: This review discusses the current progress in designing and developing polysaccharide-based biomaterials for brain tissue repair and regeneration. Polysaccharides have the potential to enhance advanced technologies in this field by interacting with a variety of biomolecules, including glycosaminoglycans and signaling moieties.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Biochemistry & Molecular Biology
Muhammad Umar Aslam Khan, Goran M. Stojanovic, Mohd Faizal Bin Abdullah, Alireza Dolatshahi-Pirouz, Hany E. Marei, Nureddin Ashammakhi, Anwarul Hasan
Summary: Tissue engineering is an advanced biomedical approach that utilizes smart hydrogels to repair and regenerate damaged tissues, treating patients with lost or failed organs or tissues. These hydrogels offer controllable properties, such as tunable mechanical characteristics and biocompatibility, making them ideal for tissue engineering.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Polymer Science
Sabrina Kopf, Dan Akesson, Mikael Skrifvars
Summary: Polyhydroxyalkanoates (PHAs) have great potential in biomedical applications, especially tissue engineering, due to their superior biocompatibility and biodegradability. Textile fiber-based tissue engineering scaffolds can mimic the properties of native tissues, and combining them with PHAs can improve performance. However, the complex crystallization behavior of PHAs and the resulting spinning procedures are still challenging. Additionally, the biomedical application of PHAs is hindered by insufficient polymer purity.
Review
Chemistry, Multidisciplinary
Federico Fontana, Fabrizio Gelain
Summary: The advent of supramolecular biopolymers has brought promising opportunities for tissue engineering, due to their tailorability, absence of toxic byproducts, and better mimicry of extracellular matrix nanotopography and mechanical properties. However, there is still a lack of understanding of their properties at meso- and macroscales. In this review, molecular modeling approaches are discussed, highlighting their advantages and limitations in representing and transferring the properties of supramolecular biopolymers.
NANOTECHNOLOGY REVIEWS
(2022)
Review
Polymer Science
Mattia Bartoli, Erik Piatti, Alberto Tagliaferro
Summary: The development of new scaffolds and materials for tissue engineering is a wide and open realm of material science. Among solutions, the use of biopolymers represents a particularly interesting area of study due to their great chemical complexity that enables creation of specific molecular architectures. However, biopolymers do not exhibit the properties required for direct application in tissue repair-such as mechanical and electrical properties-but they do show very attractive chemical functionalities which are difficult to produce through in vitro synthesis. The combination of biopolymers with nanostructured carbon fillers could represent a robust solution to enhance composite properties, producing composites with new and unique features, particularly relating to electronic conduction. In this paper, we provide a review of the field of carbonaceous nanostructure-containing biopolymer composites, limiting our investigation to tissue-engineering applications, and providing a complete overview of the recent and most outstanding achievements.
Article
Biochemistry & Molecular Biology
Jesus Chato-Astrain, David Sanchez-Porras, Oscar Dario Garcia-Garcia, Claudia Vairo, Maria Villar-Vidal, Silvia Villullas, Indalecio Sanchez-Montesinos, Fernando Campos, Ingrid Garzon, Miguel Alaminos
Summary: This study evaluated the use of EGF-loaded nanostructured lipid carriers (NLC) to enhance efficiency of human skin keratinocyte primary cultures, showing that NLC-rhEGF culture medium significantly improved cell growth and gene expression.
Article
Biochemical Research Methods
Marie C. Payne, SumYat Ho, Takao Hashimoto, Sara Imboden, Johnny A. Diaz, Brandon S. S. Lee, Melissa J. Rupert, Nathan Y. Y. Cai, Andrew S. Goldstein, Neil Y. C. Lin
Summary: The study presents a Microwell Flow Device (MFD) that generates in-well laminar flow around 3D tissues to study cancer progression and therapeutic development. The results show that the spheroids cultured in the MFD exhibit improved cell growth, reduced necrotic core formation, enhanced structural integrity, and downregulated expression of cell stress genes. The flow-cultured spheroids also exhibit an improved sensitivity to chemotherapy and greater transcriptional response. This platform enables study into hypoxia modulation, cancer metabolism, and drug screening within pathophysiological conditions.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Agricultural Engineering
Vipin Chandra Kalia, Sanjay Kumar Singh Patel, Ramasamy Shanmugam, Jung-Kul Lee
Summary: Plastics, being an integral part of daily life, are causing environmental issues due to indiscriminate use and disposal. Biodegradable polymers, especially PHAs, are considered as potential alternatives to counter this problem, with promising commercial viability, particularly in the medical sector.
BIORESOURCE TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Justina A. Ugwah, Dr Niall Savage, Dr Walter Messina, Dr Brian D. O. Donnell, Dr Martin J. O'Sullivan, Dr Eric J. Moore
Summary: A gold dual-microelectrode bioimpedance sensor was designed, fabricated, and validated for potential application in cancer diagnostics. The sensor successfully differentiated between different tissue types and provided novel data for cancer diagnostics. Testing on meat and a gel/lard phantom demonstrated excellent electrochemical recording potential of the sensor.
Article
Chemistry, Analytical
Yineng Wang, Xi Cao, Walter Messina, Anna Hogan, Justina Ugwah, Hanan Alatawi, Ed van Zalen, Eric Moore
Summary: Capillary electrochromatography (CEC) combines liquid chromatography (LC) and capillary electrophoresis (CE), providing rapid separations, high efficiency, and high selectivity. The use of capacitively coupled contactless conductivity detection (CD-D-4) allows for contactless electrode configuration, preventing contamination.
Article
Chemistry, Medicinal
S. Analakkattillam, V. K. Langsi, J. P. Hanrahan, E. Moore
Summary: A simple and robust HPLC method was developed for dissolution studies of CBD in hemp oil products for the first time, with in-house validated assay test and comparison of dissolution profiles from products purchased in the US and Europe. The study also investigated the effect of CBD release percentage on agitation speed and dissolution runtime.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Chemistry, Analytical
Netsirin Gissawong, Supalax Srijaranai, Suthasinee Boonchiangma, Pikaned Uppachai, Kompichit Seehamart, Sakwiboon Jantrasee, Eric Moore, Siriboon Mukdasai
Summary: A highly sensitive and novel electrochemical sensor has been developed for the detection of ciprofloxacin by combining gold nanoparticles deposited with waste coffee ground activated carbon and supramolecular solvent. The sensor showed good electrocatalytic activity and achieved high recoveries and low relative standard deviations in milk samples. The developed method was successfully applied in the analysis of pharmaceutical formulations and compared with high-performance liquid chromatography.
Article
Chemistry, Analytical
Thanih Balbaied, Eric Moore
Summary: The study proposed a method using biosensor-based strategies for real-time monitoring of ALP release from human colon cancer cells, facilitating understanding of cancer progression and emerging diseases. Results showed a correlation between cell response and changes in impedance magnitude and current, influencing cell morphology and viability. The developed method represents an advance over current techniques and may contribute to microfluidic testing development for small-scale techniques.
ANALYTICAL LETTERS
(2022)
Article
Chemistry, Analytical
Emma O'Sullivan-Carroll, Stewart Howlett, Carmel Pyne, Paul Downing, Ana Rafael, Marguerite Lynch, Anna Maria Hogan, Eric J. Moore
Summary: The detection of pharmaceuticals in surface and wastewater is becoming increasingly important for environmental monitoring due to the devastating effects caused by pharmaceutical release. Capillary electrophoresis coupled with solid-phase extraction provides effective detection of pharmaceutical contaminants in water.
ANALYTICAL LETTERS
(2022)
Article
Biochemical Research Methods
Hanan Alatawi, Anna Hogan, Ibtihaj Albalawi, Emma O'Sullivan-Carroll, Yineng Wang, Eric Moore
Summary: In this study, a simple and fast method using capillary electrophoresis and microchip electrophoresis with capacitively coupled contactless conductivity detection was developed for the detection and separation of paracetamol and its hydrolysis product. The method showed rapid analysis time, high efficiency and precision, low cost, and reduced consumption of reagents. This microchip system proved to be an excellent analytical technique for fast and reliable environmental applications.
Article
Chemistry, Analytical
Samia Alsefri, Thanih Balbaied, Eric Moore
Summary: Polychlorinated biphenyls (PCBs) are highly toxic synthetic chemical compounds that can cause serious ailments to living organisms. The development of an electrochemical immunosensor for PCB detection discussed in this paper offers potential for rapid sample screening in a portable, disposable format for effective control and prevention of PCBs in the environment.
Article
Biochemical Research Methods
Hanan Alatawi, Anna Hogan, Ibtihaj Albalawi, Emma O'Sullivan-Carroll, Samia Alsefri, Yineng Wang, Eric Moore
Summary: A simple and rapid method using CE and microchip electrophoresis has been developed for the separation of four nonsteroidal anti-inflammatory drugs (NSAIDs) in environmental samples. The method showed excellent repeatability, good sensitivity, low cost, high sample throughputs, portable instrumentation, and extremely lower reagent and sample consumption.
Article
Biochemistry & Molecular Biology
Apu Sarwar, Conor McSweeney, Mark Smith, Eric Moore
Summary: This study aims to identify two critical components required for pharmaceutical cleaning verification when an FTIR is used: the number of scans required per hard-to-clean location, and the limit of quantification (LOQ) of the FTIR instrument when measuring surface contamination. The current practice in pharmaceutical manufacturing does not require multiple samples, but this study suggests that collecting a sufficient number of samples is necessary to ensure measurement results are close to the true value. Furthermore, the study addresses the complexity in calculating the LOQ when dealing with complex experimental data.
Article
Chemistry, Analytical
Ibtihaj Albalawi, Hanan Alatawi, Samia Alsefri, Eric Moore
Summary: In this study, a new in situ synthesis approach was employed to detect carbaryl species using a disposable electrode, which showed superior sensitivity and selectivity for measuring carbaryl concentration in river and tap water samples.
Article
Multidisciplinary Sciences
Sandhyarani Analakkattillam, Victor K. Langsi, John P. Hanrahan, Eric Moore
Summary: A simple and validated RP-HPLC method has been developed for determining the levels of cannabidiol and tetrahydrocannabinol in hemp oil infused products. The method meets regulatory requirements and can be used for regular quality control and assay purposes.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Samia Alsefri, Thanih Balbaied, Hanan Alatawi, Ibtihaj Albalawi, Anna Hogan, Eric Moore
Summary: PCBs have been detected in soil due to industrial pollution in the past two decades, despite being banned. Therefore, it is crucial to develop an analytical method to identify and monitor the sources of PCBs. This study presents a gas chromatography with mass spectrometry technique using the QuEChERS method to effectively extract and detect PCBs in soil samples. The method was found to be simple, sensitive, efficient, and environmentally friendly.
Article
Chemistry, Analytical
Apu Sarwar, Conor McSweeney, Joep Timmermans, Eric Moore
Summary: Preventing cross-contamination is a regulatory requirement in the pharmaceutical manufacturing industry. The use of real-time infrared spectroscopy can accurately measure surface cleanliness in pharmaceutical manufacturing.
Article
Chemistry, Analytical
Ibtihaj Albalawi, Anna Hogan, Hanan Alatawi, Eric Moore
Summary: This study introduces a simple, low-cost, sensitive, and environmentally friendly approach for simultaneous electrochemical determination of lead and cadmium using a commercial screen-printed gold electrode with Nafion and in situ bismuth film. The developed sensor showed high sensitivity and good repeatability with detection limits below EPA limits, making it suitable for analysis of river and tap water samples. By using anodic stripping square voltammetry, cyclic voltammetry, and scanning electron microscopy, the electrochemical properties and composite film characteristics were investigated.
SENSING AND BIO-SENSING RESEARCH
(2021)
