Journal
ANALYTICAL CHEMISTRY
Volume 88, Issue 16, Pages 8339-8345Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.6b02740
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Funding
- National Natural Science Foundation of China [21135002, 21361162002, 21575083]
- Priority Development Areas of The National Research Foundation for the Doctoral Program of Higher Education of China [20130091130005]
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This work proposes the first photoelectrochemical proximity assay (PECPA) method via the sensitization of CdTe quantum dots (QDs) on photoelectrochemical response of ITO/TiO2/CdS electrode for highly selective and sensitive detection of proteins. This detection was performed on a sensing interface-formed via the hybridization of capture DNA immobilized on,ITO/TiO2/CdS electrode with labeled antibody-DNA :(DNA-Abl). Upon the recognition of Abl to target protein, the,immunocomplex of DNA-Abl, target, and the detection antibody,-DNA (DNA-Ab2) was formed; which led to the proximity hybridization of the DNA in DNA-Ab2, capture DNA, and signal DNA-CdTe QDs, and brought CdTe QDs to the ITO/TiO2/CdS electrode to produce a sensitized photocurrent. The photocurrent intensity increased With the increasing concentration of the specific target protein. Using insulin as a target, this sensitized method showed a detectable range of 10 fM to 10 nM and a detection limit of 3:0 fM without the need of a washing step. It possessed high selectivity and,good accuracy for detection Of proteins in biological matrixes. This method is extremely flexible and can be extended to varieties of protein targets.
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