Article
Chemistry, Analytical
Mengxia Cheng, Hong Shu, Ye Peng, Xiaoxiao Feng, Guoquan Yan, Lei Zhang, Jun Yao, Huimin Bao, Haojie Lu
Summary: A comprehensive strategy combining linkage-specific derivatization and charge-sensitive separation method was developed for specific analysis of alpha-2,3-sialylated N-glycan linkage isomers. This new approach demonstrated successful sensitive profiling and reproducible quantitation of serum alpha-2,3-sialylated N-glycome from ovarian cancer patients, identifying significant changes in certain glycan structures.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Michael McCullagh, Severine Goscinny, Martin Palmer, Jakub Ujma
Summary: The article discusses the use of LC-MS for pesticide residue screening, highlighting the presence of charge-site isomers and their impact on relative abundances of product ions, which led to the integration of IMS technology to address this issue. The authors utilized different IM devices to resolve charge-site isomers of various pesticides.
Article
Chemistry, Analytical
Katerina V. Djambazova, Martin Dufresne, Lukasz G. Migas, Angela R. S. Kruse, Raf Van de Plas, Richard M. Caprioli, Jeffrey M. Spraggins
Summary: Gangliosides are diverse acidic glycosphingolipids with distinct biological roles. Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) can be used for spatial analysis of gangliosides directly from tissue sections. The integration of trapped ion mobility spectrometry with MALDI IMS enables the analysis of isomeric lipid structures in situ. Gas-phase separation and identification of disialoganglioside isomers GD1a and GD1b, with different sialic acid residue positions, were demonstrated in multiple samples, including tissue sections.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Yang Li, Yujiao Qin, Songchang Wei, Ling Ling, Chuan-Fan Ding
Summary: Steroids are important indicators of health and disease. Differentiating between steroids, especially their isomers, can be challenging due to their structural similarities. This study presents a trapped ion mobility spectrometry-mass spectrometry (TIMS-MS) approach based on steroid analogue adduction for specific identification of isomers. The use of dexamethasone (DEX) as a heterodimer enhancer allowed successful separation of isomer pairs in TIMS.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
A. Bianco, I Neefjes, D. Alfaouri, H. Vehkamaki, T. Kurten, L. Ahonen, M. Passananti, J. Kangasluoma
Summary: Mass spectrometry is an effective method for identifying and quantifying environmentally relevant molecules, but it cannot distinguish between isomers. This study combines an experimental set-up using a differential mobility analyser with computational ion mobility calculations to successfully identify isomers. The experimental set-up allows for analysis in both the gas and liquid phase, making it a versatile tool for isomer separation.
Article
Biochemical Research Methods
Zuzana Stiffelova, Ladislav Moravsky, Bartosz Michalczuk, Jozef Cizmarik, Stefan Matejcik, Fils Andriamainty
Summary: This study demonstrated the separation of positional isomers of alkoxyphenylcarbamic acid derivatives using Thin Layer Chromatography (TLC) and Ion Mobility Spectrometry (IMS) with diode laser desorption. Ion mobilities increased with the change in geometry from ortho to para position of the alkoxy chain, consistent with their different collision cross sections (CCS).
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Chemistry, Analytical
Jieqiang Zhong, Yifan Huang, Yehia Mechref
Summary: This study presents an efficient derivatization method for discriminating sialic acid linkage isomers on glycopeptides, resulting in increased signal and successful characterization of sialylated glycopeptides from various proteins. The method targets site- and sialic acid linkage-specific profiling of glycosylation, making it a facile and highly reproducible technique.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Qingyu Hu, Yuting Sun, Xiyan Mu, Yulan Wang, Huiru Tang
Summary: Direct-infusion tandem mass spectrometry (DI-MS/MS) is valuable for quantitative metabolomics, MS imaging, and single cell studies, but cannot differentiate isomers/isobars with similar MS spectral features. In this study, the fragmentation pathways and characteristics of differential ion-mobility spectrometry (DMS) for four organic compounds were comprehensively investigated. A DI-DMS-MS/MS method was developed to simultaneously quantify these compounds. The method showed high accuracy and low limit-of-quantification, and clear differences between human and mouse urine samples were observed.
Article
Chemistry, Analytical
Carlo Roberto de Bruin, Marie Hennebelle, Jean -Paul Vincken, Wouter J. C. de Bruijn
Summary: In this study, cyclic ion mobility mass spectrometry (cIMS-MS) was used to separate catechin epimers and a fast method for their identification and semiquantification was developed. This method can be applied for the semi-quantification of catechin epimers in food samples, such as tea.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Mei Li, Wei Huang, Hong Chen, Dandan Jiang, Weiguo Wang, Yao Xiao, Chuang Chen, Haiyang Li
Summary: The DAPI-IMS technique utilizes a dopant and NH3 sample gas to achieve specific and sensitive detection of NH3 in high humidity and complex matrix background, with a linear response range of 20-800 ppb(v) and a sensitivity of 0.20 fC/ppb(v).
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Aurelie Delvaux, Estelle Rathahao-Paris, Blanche Guillon, Sophie Cholet, Karine Adel-Patient, Francois Fenaille, Christophe Junot, Sandra Alves
Summary: The study introduces a high throughput and high resolution method for characterizing main oligosaccharides in breastmilk using ion mobility spectrometry, offering high identification level. Standard HMO isomers were investigated and could be distinguished effectively. Multiple mobility peaks were observed for some HMO species, but reduction at the reducing-end did not significantly improve isomer distinction.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Shon P. Neal, Walker N. Hodges, Diana C. Velosa, Ralph Aderorho, Shadrack Wilson Lucas, Christopher D. Chouinard
Summary: In the last decade, the use of ion mobility-mass spectrometry (IM-MS) has grown rapidly, but the limited resolving power has been a challenge. This study explores the use of chemical reactions, specifically derivatization with Girard's reagents and 1,1-carbonyldiimidazole (CDI), to improve the separation of isomers of steroid hormones using IM-MS. The results show that these reactions coupled with IM-MS significantly improve the resolution of challenging isomer groups, allowing for more accurate and efficient analysis of complex mixtures.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
O. Tara Liyanage, Ana Quintero, Jacob B. Hatvany, Elyssia S. Gallagher
Summary: This study presents a rapid hydrogen/deuterium exchange mass spectrometry method for analyzing solvated carbohydrates, by adjusting the reaction time through modifying the spray-solvent conductivity. The research demonstrates that monitoring hydrogen/deuterium exchange at multiple time points allows for the differentiation of three trisaccharide isomers with distinct structures.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
Chongqing Ma, Tianrong Yu, Yue Liu, Rui Shi, Rui Lv, Ruochen Guo, Qinghua Cao, Gaole Dai, Yu Zhao, Jian Liu
Summary: This study presents a novel method for cationizing neutral small molecules using a specific compound as a new MALDI matrix, enabling efficient and clear detection of oligosaccharides and amino acids. This approach breaks through traditional organic matrix cationization methods and provides inspiration for the development of next-generation matrices for sensitive detection of hard-to-ionize molecules.
Article
Biochemical Research Methods
Orobola E. Olajide, Benedicta Donkor, Ahmed M. Hamid
Summary: This study optimized the coupling of ambient ionization techniques with ion mobility mass spectrometry, resulting in high sensitivity and selectivity for sample analysis. A novel approach for collision cross section measurements was introduced, allowing confident identification of analyte structures. The results suggest that this coupling enables rapid, sensitive, and highly selective analysis that can be used in various fields, such as agrochemical screening and disease diagnostics.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)