Journal
ANALYTICAL CHEMISTRY
Volume 88, Issue 14, Pages 7302-7311Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.6b01655
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Funding
- National Institutes of Health/National of General Medical Sciences [5P41 GM103391-05]
- National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Diseases [F32 FDK105841A]
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Spatial resolution is a key parameter in imaging mass spectrometry (IMS). Aside from being a primary determinant in overall image quality, spatial resolution has important consequences on the acquisition time of the IMS experiment and the resulting file size. Hardware and software modifications during instrumentation development can dramatically affect the spatial resolution achievable using a given imaging mass spectrometer. As such, an accurate and objective method to determine the working spatial resolution is needed to guide instrument development and ensure quality IMS results. We have used lithographic and self-assembly techniques to fabricate a pattern of crystal violet as a standard reticle slide for assessing spatial resolution in matrix-assisted laser desorption/ionization (MALDI) IMS experiments. The reticle is used to evaluate spatial resolution under user-defined instrumental conditions. Edgespread analysis measures the beam diameter for a Gaussian profile and line scans measure an effective spatial resolution that is a convolution of beam optics and sampling frequency. The patterned crystal violet reticle was also used to diagnose issues with IMS instrumentation such as intermittent losses of pixel data.
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