Journal
ANALYTICAL CHEMISTRY
Volume 88, Issue 12, Pages 6292-6300Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.6b00477
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Funding
- Centre National de la Recherche Scientifique (CNRS)
- Wallonie-Bruxelles International
- Fonds de la Recherche Scientifique
- Ministry of Foreign Affairs
- Ministry of Higher Education and Research
- Hubert Curien Partnerships [29120QE]
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The redox couple resazurin-resorufin exhibits electrofluorochromic properties which are investigated herein by absorption and fluorescence spectroelectrochemistry and by electrochemically coupled fluorescence confocal laser scanning microscopy (EC-CLSM). At pH 10, the highly fluorescent resorufin dye is generated at the electrode surface by the electrochemical reduction of the poorly fluorescent resazurin. Performing EC-CLSM at electrode surfaces allows to monitor spatially resolved electrochemical processes in situ and in real time. Using a small (315 mu m diameter) cylindrical electrode, a steady-state diffusion layer builds up under potentiostatic conditions at -0.45 V vs AglAgCl. Mapping the fluorescence intensity in 3D by CLSM enables us to reconstruct the relative concentration profile of resorufin around the electrode. The comparison of the experimental diffusion-profile with theoretical predictions demonstrates that spontaneous convection has a direct influence on the actual thickness of the diffusion layer, which is smaller than the value predicted for a purely diffusional transport. This study shows that combining fluorescence CLSM with electrochemistry is a powerful tool to study electrochemical reactivity at a spatially resolved level.
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