Review
Chemistry, Analytical
Xiaoping Wang, Hui Li, Kaijun Quan, Liang Zhao, Hongdeng Qiu, Zuguang Li
Summary: Separation and identification of chiral enantiomers are increasingly important in various fields like pharmaceutical production, chemical intermediates preparation, and biochemistry. Cellulose and its derivatives, with strong chiral recognition ability, large loading capacity and ease of functionalization, have shown excellent performance in enantioseparation, indicating great potential in chiral separation. This review systematically summarizes the types of cellulose derivatives, preparation of cellulose-functionalized chiral stationary phases, and their recent applications in chiral chromatographic separation, aiming to provide a useful reference for researchers and inspire new discoveries in the field.
Article
Biochemical Research Methods
Xiaoping Wang, Hui Li, Kaijun Quan, Liang Zhao, Zuguang Li, Hongdeng Qiu
Summary: In this study, a series of excellent chiral stationary phases were developed using anhydride modified beta-cyclodextrin for enantioseparation, demonstrating good resolution and repeatability. These new CSPs show great potential for chiral separation in actual applications.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Multidisciplinary Sciences
Mikhail A. A. Kuznetsov, Sergey M. M. Staroverov, Nikita Sarvin, Ruslan Puzankov, Pavel N. N. Nesterenko
Summary: This study investigated the retention regularities and separation enantioselectivity of selected beta-blockers using eight chiral stationary phases in a polar ionic mode elution system. The results showed that the presence of a free carboxylic group in the chiral selector molecule was crucial for the retention of enantiomers. The best enantioseparation of beta-blockers was achieved with chiral selectors containing eremosaminyl eremomycin aglycon and des-eremosamynyl eremomycin.
Article
Chemistry, Analytical
Bin Huang, Kuan Li, Qi-Yu Ma, Tuan-Xiu Xiang, Rui-Xue Liang, Ya-Nan Gong, Bang-Jin Wang, Jun-Hui Zhang, Sheng-Ming Xie, Li-Ming Yuan
Summary: This study demonstrates the use of metallacycles as stationary phases for gas chromatographic (GC) separations. The metallacycle-coated column showed excellent separation performance for various organic isomers and racemic compounds. This work highlights the potential of metallacycles in GC separations and expands their applications in separation science.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Medicinal
Mahmoud A. Tantawy, Hassan Y. Aboul-Enein, Ali M. Yehia
Summary: Chiral HPLC technique with polysaccharide-based stationary phases is often wasteful in terms of organic solvents. Shorter columns and high water mobile phases are commonly used to make the method more environmentally friendly. This study developed a new chiral chromatographic technique using cellulose-based columns to successfully separate phenylephrine HCl and guaifenesin racemates in a reversed phase mode.
Article
Chemistry, Multidisciplinary
A. A. Tumashov, S. A. Vakarov, L. Sh Sadretdinova, E. N. Chulakov, G. L. Levit, V. P. Krasnov, V. N. Charushin
Summary: The study explored the separation of enantiomers of several 2-aryloxycarboxylic acids using normal- and reversed-phase HPLC on popular chiral stationary phases. The best separation parameters were achieved with Chiralcel OD-H and Chiralpack AD under normal-phase HPLC conditions. Preparative chiral HPLC successfully isolated the (S)- and (R)-enantiomers of 2-(1-naphthyloxy)- and 2-(2-iodophenoxy)propionic acids with enantiomeric excess ee >99%.
RUSSIAN CHEMICAL BULLETIN
(2021)
Article
Biochemical Research Methods
Wei Chen, Ji-Zhou Jiang, Guo-Song Qiu, Sheng Tang, Zheng-Wu Bai
Summary: The present study aimed to investigate the interactions between chitosan-type chiral selectors and chiral analytes in enantioseparation. Through synthesizing and characterizing chitosan derivatives and evaluating their enantioseparation capability, the study highlighted the importance of substituents on the phenyl group in chiral recognition mechanism. The study also shed light on the role of hydrogen bonding formation in enantioseparation and the impact of different mobile phases on the process.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemistry & Molecular Biology
John M. M. Ferraro, Weston J. J. Umstead
Summary: Until recently, chirality has not been a major focus in the study of cannabinoids. However, with the discovery of more cannabinoids, chiral compounds like cannabichromene and cannabicyclol have emerged as potential investigatory candidates. The study aimed to identify analytical separation conditions and adapt them for preparative separation, using column screening on Daicel's immobilized polysaccharide chiral stationary phases. The results showed that CHIRALPAK(R) IK was able to successfully separate all four compounds.
Article
Chemistry, Applied
D. S. Prosuntsova, A. Yu Plodukhin, I. A. Ananieva, E. K. Beloglazkina, P. N. Nesterenko
Summary: A new composite chiral stationary phase for high-performance liquid chromatography was prepared using gold nanoparticles coated polystyrene-divinylbenzene microspherical particles, with l-lysine conjugate with lipoic acid utilized for enhancing chiral selectivity. The immobilization of the chiral selector was achieved by the reaction of sulfur-containing groups from lipoic acid residue with the gold surface of nanoparticles, enabling successful enantiomers separation for flurbiprofen and ketoprofen.
JOURNAL OF POROUS MATERIALS
(2021)
Review
Chemistry, Medicinal
David Jin Han Seog, Nguyen van Kien, Jae Jeong Ryoo
Summary: This updated minireview focuses on chiral stationary phases (CSPs) based on amino alcohols. The study highlights the use of amino alcohols as starting materials for the preparation of chiral catalysts in asymmetric organic synthesis and CSPs for chiral separations. Important developments and applications of various CSPs, including Pirkle-type CSPs, ligand exchange CSPs, alpha-amino acid-derived amino alcohol CSPs, and symmetric CSPs, are summarized to propose ideas for developing new CSPs with improved performance.
Review
Biochemical Research Methods
Huifeng Liu, Zhihai Wu, Jia Chen, Jianhua Wang, Hongdeng Qiu
Summary: Chirality is a common phenomenon in nature and the separation of chiral drugs is crucial for their safe utilization. High-performance liquid chromatography (HPLC) is an effective tool for compound separation and analysis, with chromatographic packing playing a key role. Chiral pharmaceutical separation and analysis in HPLC rely on chiral stationary phases (CSPs), thus various CSPs are being developed to meet the needs of chiral drug separation and analysis.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Applied
Hongzhong Deng, Xuepeng Wu, Lili Zhang, Jun Shen, Yingjie Qiao, Xiaodong Wang, Chengying Bai, Ting Zheng, Yoshio Okamoto
Summary: Chitosan derivatives with various substituents were prepared and their enantioseparation properties were examined. One specific derivative showed attractive chiral recognition abilities, especially for dihydropyridine calcium antagonist racemates.
CARBOHYDRATE POLYMERS
(2022)
Review
Chemistry, Analytical
Ester Lubomirsky, Aminreza Khodabandeh, Jasmin Preis, Moritz Susewind, Thorsten Hofe, Emily F. Hilder, R. Dario Arrua
Summary: Synthetic and natural macromolecules play significant roles in various fields, and characterizing their structural parameters is essential for understanding structure-property relationships. Size exclusion chromatography is a commonly used technique for polymer characterization, and advancements in stationary phase design and synthetic methods can enhance its efficiency in macromolecule separation.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Yuanyuan Pei, Xinyu Li, Guangjian Zeng, Yuting Gao, Tao Wen
Summary: Two novel chiral stationary phases (CSPs) based on lactide derivatives, PMLA and N-PMLA, showed better chiral recognition ability compared to PLLA in both reversed-phase and normal-phase chromatographic modes in this study.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Review
Polymer Science
Guanzhao Wu
Summary: Chiral separation by HPLC using a CSP is a reliable method for obtaining pure enantiomers. Helical polyacetylene derivatives show excellent chiral recognition abilities and are promising CSP candidates for HPLC. This review discusses the development of helical polyacetylene derivatives with different chiral pendants and their potential as coated CSPs for enantioseparation in HPLC.
Article
Biochemical Research Methods
Pierpaolo Tomai, Andrea Martinelli, Tecla Gasperi, Maria Bianchi, Velia Purcaro, Luciana Teofili, Patrizia Papacci, Maria Sofia Cori, Giovanni Vento, Roberta Curini, Salvatore Fanali, Alessandra Gentili
JOURNAL OF CHROMATOGRAPHY A
(2019)
Article
Biochemical Research Methods
Giovanni D'Orazio, Chiara Fanali, Alessandra Gentili, Salvatore Fanali
Correction
Biochemical Research Methods
Pierpaolo Tomai, Andrea Martinelli, Tecla Gasperi, Maria Bianchi, Velia Purcaro, Luciana Teofili, Patrizia Papacci, Maria Sofia Coli, Giovanni Vento, Roberta Curini, Salvatore Fanali, Alessandra Gentili
JOURNAL OF CHROMATOGRAPHY A
(2019)
Article
Chemistry, Analytical
Alessandra Gentili, Chiara Dal Bosco, Salvatore Fanali, Chiara Fanali
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2019)
Review
Biochemical Research Methods
Salvatore Fanali, Bezhan Chankvetadze
Editorial Material
Biochemical Research Methods
Salvatore Fanali, Shaoping Li
JOURNAL OF CHROMATOGRAPHY A
(2019)
Review
Pharmacology & Pharmacy
Chiara Fanali, Giovanni D'Orazio, Alessandra Gentili, Salvatore Fanali
Summary: This review paper discusses the application of miniaturized techniques, including capillary electrophoresis and nano-liquid chromatography, in the separation and analysis of NSAIDs, highlighting their high mass sensitivity and potential for coupling with Mass Spectrometry.
CURRENT PHARMACEUTICAL ANALYSIS
(2021)
Article
Biochemical Research Methods
Giovanni D'Orazio, Chiara Fanali, Salvatore Fanali, Alessandra Gentili, Marina Karchkhadze, Bezhan Chankvetadze
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Biochemical Research Methods
Pierpaolo Tomai, Alessandra Gentili, Salvatore Fanali, Yolanda Pico
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Biochemical Research Methods
Salvatore Fanali, Bezhan Chankvetadze
Summary: Capillary electrochromatography (CEC) has gone through a dramatic development from promise to growth to stagnation and decline. Various reasons such as competitive trends, inadequate development, and lack of innovative activities have led to the stagnation of CEC in the field.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Review
Biochemical Research Methods
Chiara Fanali, Giovanni D'Orazio, Alessandra Gentili, Salvatore Fanali
Summary: Capillary electromigration and liquid chromatographic techniques are miniaturized methods that offer advantages in separation science with high efficiency and resolution. They have been widely used for the analysis of various compounds and show potential for drug abuse analysis.
Article
Biochemical Research Methods
Massimo Giuseppe De Cesaris, Giovanni D'Orazio, Chiara Fanali, Alessandra Gentili, Nino Takaishvili, Bezhan Chankvetadze, Salvatore Fanali
Summary: In this study, enantiomers of chiral agrochemicals were successfully separated using nano-liquid chromatography and capillary electrochromatography. The use of a capillary column packed with immobilized amylose tris(3-chloro-5-methylphenylcarbamate) as the chiral selector showed better performance in CEC than in nano-LC. The findings suggest the potential of CEC for enantioseparations in the analysis of chiral agrochemicals.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Elena Lucci, Chiara Dal Bosco, Lorenzo Antonelli, Chiara Fanali, Salvatore Fanali, Alessandra Gentili, Bezhan Chankvetadze
Summary: Up to 40% of plant protection products currently used contain chiral active ingredients, which may exhibit different activity, toxicity, and biotic degradation rates due to their interaction with enzymes or other naturally occurring asymmetric molecules. High-performance liquid chromatography (HPLC) is commonly employed for enantioseparations of chiral pesticides, with derivatized amylose/cellulose-based columns being the most widely used due to their universal analyte coverage. Trace analysis using sensitive detection techniques such as mass spectrometry has revealed the ubiquitous occurrence of chiral pesticides in various environmental matrices. The research in this field has grown significantly in recent years, driven by the need to assess and mitigate the risks associated with exposure to these emerging contaminants.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Review
Chemistry, Analytical
Susanna Della Posta, Chiara Fanali, Valeria Gallo, Salvatore Fanali
Summary: This paper presents recent results obtained by coupling electromigration techniques with mass spectrometry, highlighting the advantages of this hybrid approach and discussing selected applications.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
