Article
Microbiology
Ineta Kalnina, Dita Gudra, Ivars Silamikelis, Kristine Viksne, Ance Roga, Edmunds Skinderskis, Davids Fridmanis, Janis Klovins
Summary: This study investigates the relationship between the composition of the gut microbiome and variations in lipid profiles among healthy adults. The findings suggest that the gut microbiome plays a role in regulating host lipid metabolism, with different bacterial genera associated with different lipid patterns.
Article
Chemistry, Multidisciplinary
Xiangyu Guo, Wenbo Cao, Xiaomin Fan, Zhiying Guo, Donghui Zhang, Haoyue Zhang, Xiaoxiao Ma, Jiahong Dong, Yunfang Wang, Wenpeng Zhang, Zheng Ouyang
Summary: Mass spectrometry imaging (MSI) of lipids in biological tissues can provide valuable information for biological research and disease diagnosis by correlating molecular distribution with pathological results. A new multiplexed MS2 imaging method (MSI-I-2) has been developed, allowing for MS2 images of 10 or more lipid species at each sampling spot, providing spatial structural lipidomic information. Coupled with on-tissue photochemical derivatization, imaging of 20 phospholipid C=C location isomers is also possible, resulting in enhanced molecular images with high structural definition for mouse brain and human liver cancer tissue sections. Spatially mapped t-distributed stochastic neighbor embedding has been utilized to visualize the tumor margin with the assistance of structural lipidomic information.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Multidisciplinary Sciences
Ntsiki M. Held, M. Renate Buijink, Hyung L. Elfrink, Sander Kooijman, Georges E. Janssens, Angela C. M. Luyf, Mia L. Pras-Raves, Frederic M. Vaz, Stephan Michel, Riekelt H. Houtkooper, Michel van Weeghel
Summary: The study reveals that lipid metabolism is controlled by the circadian system, and aging affects the rhythmicity of lipid metabolism, leading to a decrease in oscillating lipids with age. While lipid accumulation increases with aging, the percentage of rhythmic lipids decreases, and the diurnal profiles of lipids in brown adipose tissue may be dependent on the length of the lipid acyl chain, a regulation that is lost in aged mice.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Carmen Pena-Bautista, Lourdes Alvarez-Sanchez, Ines Ferrer, Marina Lopez-Nogueroles, Antonio Jose Canada-Martinez, Camille Oger, Jean-Marie Galano, Thierry Durand, Miguel Baquero, Consuelo Chafer-Pericas
Summary: Alzheimer's Disease (AD) is a common neurodegenerative disease in aging populations, with complex diagnosis usually performed in advanced stages. Plasma isoprostanoids may serve as useful biomarkers for preclinical diagnosis of AD, as shown in the study, but further validation is warranted.
Article
Radiology, Nuclear Medicine & Medical Imaging
Miriam Mengoni, Andreas Dominik Braun, Mattes Simon Hinnerichs, Thomas Tuting, Alexey Surov
Summary: This study aims to evaluate radiologic measurements of body composition as biomarkers predicting treatment response and adverse events (AEs) in melanoma patients receiving immune checkpoint inhibitors (ICI). The study found that low subcutaneous adipose tissue gauge index (SATGI) was associated with prolonged progression-free survival (PFS) and enhanced objective response rate. In addition, a significant enrichment of cases with vitiligo was observed in the SATGI-low cohort.
ACADEMIC RADIOLOGY
(2023)
Review
Spectroscopy
Siddabasave Gowda B. Gowda, Chandra Shekhar, Divyavani Gowda, Yifan Chen, Hitoshi Chiba, Shu-Ping Hui
Summary: Coronavirus disease 2019 (COVID-19) is a global health threat that has rapidly spread worldwide. Understanding the role of lipid metabolism and using mass spectrometry (MS)-based methods can help develop new therapeutics for COVID-19. MS technologies are being established as efficient methods for discovering potential diagnostic biomarkers and studying lipid profile alterations in patients with COVID-19. This review summarizes the various strategies and challenges in using MS technologies for lipidomic analysis and biomarker discovery, and discusses future perspectives in drug discovery and diagnosis of COVID-19.
MASS SPECTROMETRY REVIEWS
(2023)
Article
Multidisciplinary Sciences
Sungwon Ham, Minjee Kim, Sangwook Lee, Chuan-Bing Wang, BeomSeok Ko, Namkug Kim
Summary: Semantic segmentation with transfer learning of convolutional neural networks is used to create robust breast segmentation in supine breast MRI, overcoming the challenges of breast shape variability and MRI artifacts.
SCIENTIFIC REPORTS
(2023)
Article
Pharmacology & Pharmacy
Felix F. Schmidt, Dajana Lichtenstein, Hannes Planatscher, Almut Mentz, Joern Kalinowski, Andreas E. Steinhilber, Thomas O. Joos, Albert Braeuning, Oliver Poetz
Summary: Toxicological effects of chemicals are often tested individually, while consumers are actually exposed to complex mixtures like multiple pesticide residues. Researching the combined effects of pesticides can provide a more comprehensive understanding of their impact on animals and humans.
Article
Neurosciences
Tatiana Pushkarsky, Adam Ward, Andrey Ivanov, Xionghao Lin, Dmitri Sviridov, Sergei Nekhai, Michael Bukrinsky
Summary: HIV infection may accelerate the development of neurocognitive disorders by inhibiting cholesterol efflux and affecting the functionality of lipid rafts. The presence of HIV-1 Nef correlates significantly with the severity of HAND, highlighting its contribution to the pathogenesis and suggesting a connection to Alzheimer's disease.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Endocrinology & Metabolism
Yuanye Jiang, Xiaoyu Zhuang, Jiaqi Zhang, Meng Li, Shengnan Du, Jiyun Tian, Yifu Yuan, Guang Ji, Cheng Hu
Summary: This study investigated the clinical and proteomic characteristics of lean individuals with NAFLD, and found that they exhibit distinct clinical profiles compared to overweight individuals with NAFLD. Plasma proteomic profiling revealed potential biomarkers with diagnostic value for lean NAFLD. These proteins are primarily involved in lipid metabolism, immune and complement systems, and platelet degranulation.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Oncology
Melissa Bradbury, Eva Borras, Assumpcio Perez-Benavente, Antonio Gil-Moreno, Anna Santamaria, Eduard Sabido
Summary: This manuscript reviews the management strategies and molecular advances in high-grade serous ovarian cancer, emphasizing the role of proteomics in studying tumor biology and guiding the development of new biomarkers and therapeutic targets. High-grade serous ovarian cancer remains a challenging subtype characterized by late diagnosis and frequent relapse despite standardized treatment. The integration of proteomics with genomics holds promise for improving our understanding of the disease and developing more effective treatments.
Article
Chemistry, Multidisciplinary
Stefan Lulf, Luxuan Guo, Tobias Parchomyk, Jeremy N. Harvey, Konrad Koszinowski
Summary: This study investigates the oxidative addition of organyl halides to phenylferrate anions in the gas phase, revealing the impact of different iron complexes on reactivity and reaction pathways. DFT calculations provide insights into the high reactivity of Ph2Fe(I)(-) complex.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Oncology
Bo Yu, Jizhe Wang
Summary: This study aims to identify new lipid biomarkers for the diagnosis of laryngeal cancer and to uncover their molecular characteristics. The research found that certain lipid levels significantly increased in laryngeal cancer patients, while others decreased. Lipidomics is a powerful tool for exploring abnormal lipid metabolism in laryngeal cancer, and lysophospholipids and phospholipids may serve as potential biomarkers for its diagnosis.
FRONTIERS IN ONCOLOGY
(2021)
Article
Chemistry, Analytical
Peixi Zhu, Dandan Chen, Kezhi Jiang, Siqi Zhu, Weike Su, Ann Van Schepdael, Erwin Adams
Summary: Tetracyclines and their 4-epimers can be rapidly differentiated by energy resolved mass spectrometry (ERMS) of the sodium adduct ions. The interaction between the drugs and alkali metal ions is achieved through the dimethyl amino group at the C-4 position, and the binding with the hydroxyl group at the C-5 and C-6 positions differs when in contact with lithium ions.
Article
Chemistry, Physical
M. Diop, M. El-Hayek, J. Attard, A. Muhieddine, V. Veremeienko, S. Soorkia, Ph. Carbonniere, A. de la Lande, B. Soep, N. Shafizadeh
Summary: In this study, the properties of Pheophytin a and chlorophyll a in photosynthesis were investigated using mass spectrometry. The results showed that both Pheophytin and chlorophyll were detected intensively in the protonated mode, with their main fragmentation route being the loss of the phytyl chain. In the negative mode, Pheophytin remained intact, while chlorophyll was not detected under normal conditions. The addition of methanol solvent molecule facilitated the detection of chlorophyll. Computational and experimental results indicated that the addition of methanol occurred on a double bond of deprotonated chlorophyll.
JOURNAL OF CHEMICAL PHYSICS
(2023)
Article
Cell Biology
Izabella Lice, Jose Marcos Sanches, Rebeca D. Correia-Silva, Mab P. Correa, Marcelo Y. Icimoto, Alex A. R. Silva, Salvador Sanchez-Vinces, Andreia M. Porcari, Vanessa Moreira, Cristiane D. Gil
Summary: This study evaluated the effects of Fpr1 and Fpr2 activation on acute peritonitis and macrophages. The results showed that Ac9-12 and WKYMV reduced leukocyte influx and IL-1 beta release. Additionally, these peptides regulated IL-6 production by LPS-stimulated macrophages. These findings suggest the therapeutic potential of Fpr1 or Fpr2 activation in inflammatory response and macrophage activation.
Article
Multidisciplinary Sciences
Estefenson Marques Morais, Alex Ap Rosini Silva, Francisco Wderson Araujo de Sousa, Izabelle Maria Barboza de Azevedo, Helane Franca Silva, Alice Maria Goncalves Santos, Jose Evando Aguiar Beserra Junior, Caroline Pais de Carvalho, Marcos Nogueira Eberlin, Andreia M. Porcari, Francisca Diana da Silva Araujo
Summary: The potential role of endophytic Trichoderma strains isolated from the Cerrado-Caatinga ecotone as biological control agents of crop pathogenic fungi was evaluated in this study. The results showed that these Trichoderma strains had inhibitory activity on the growth of common crop pathogenic fungi, indicating their biocontrol potential. Metabolomic analysis revealed antifungal metabolites and other bioactive molecules in the Trichoderma extracts.
Article
Oncology
Marcella R. Cardoso, Alex Ap Rosini Silva, Maria Cecilia R. Talarico, Pedro H. Godoy Sanches, Mauricio L. Sforca, Silvana A. Rocco, Luciana M. Rezende, Melissa Quintero, Tassia B. B. C. Costa, Lais R. Viana, Rafael R. Canevarolo, Amanda C. Ferracini, Susana Ramalho, Junier Marrero Gutierrez, Fernando Guimaraes, Ljubica Tasic, Alessandra Tata, Luis O. Sarian, Leo L. Cheng, Andreia M. Porcari, Sophie F. M. Derchain
Summary: This study utilized Nuclear Magnetic Resonance (NMR) to analyze serum metabolic profiles of breast cancer patients, combined with immunohistochemical parameters, to differentiate between sensitive and resistant patients to Neoadjuvant chemotherapy (NACT), achieving high prediction accuracy.
Article
Biochemistry & Molecular Biology
Salvador Sanchez-Vinces, Pedro Henrique Dias Garcia, Alex Ap. Rosini Silva, Anna Maria Alves de Piloto Fernandes, Joyce Aparecida Barreto, Gustavo Henrique Bueno Duarte, Marcia Aparecida Antonio, Alexander Birbrair, Andreia M. Porcari, Patricia de Oliveira Carvalho
Summary: The comprehensive lipidome determined by mass spectrometry is correlated to the serum whole-lipidic profile of adults with different lipidemic conditions. Specifically, serum TAG levels have a positive correlation with certain specific TAGs and ceramides, and a negative correlation with sphingomyelins. HDL-C levels are positively correlated with certain groups of glycerophosphocholine, while LDL-C has a positive correlation with sphingomyelins.
Article
Food Science & Technology
Andrea Massaro, Marco Bragolusi, Alessandra Tata, Carmela Zacometti, Stephane Lefevre, Aline Fregiere-Salomon, Jean-Louis Lafeuille, Giuseppe Sammarco, Ingrid Fiordaliso Candalino, Michele Suman, Roberto Piro
Summary: This study developed a non-targeted method for authenticating black pepper using near-infrared spectroscopy (NIR) and the LASSO method, and successfully validated its accuracy and stability. The method can classify and predict samples, and can be conveniently operated through an online application.
Article
Chemistry, Applied
Marco Bragolusi, Alessandra Tata, Andrea Massaro, Carmela Zacometti, Roberto Piro
Summary: Nutritional information on food labels can influence consumers' dietary choices. This study used near infrared spectroscopy to verify the accuracy of nutritional labels on 103 food products. The results showed that 35% of processed food products did not have nutritional labels, and some of the labeled values did not match the experimental data. Therefore, this study demonstrated the capability of near infrared spectroscopy in monitoring compliance with EU regulations and providing reference methods for further confirmation.
JOURNAL OF NEAR INFRARED SPECTROSCOPY
(2023)
Article
Microbiology
Alessandra Tata, Filippo Marzoli, Miriam Cordovana, Alessia Tiengo, Carmela Zacometti, Andrea Massaro, Lisa Barco, Simone Belluco, Roberto Piro
Summary: This study developed and validated a method to automatically serotype Legionella pneumophila and distinguish it from Legionella non-pneumophila using Fourier-transform infrared spectroscopy (FT-IR) and machine learning. Data validation strategies were employed to ensure consistent performance of the method across a large microbial population. The method achieved high accuracy on different test sets from European collections.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Food Science & Technology
Carmela Zacometti, Alessandra Tata, Andrea Massaro, Giorgia Riuzzi, Marco Bragolusi, Giulio Cozzi, Roberto Piro, Sara Khazzar, Gabriele Gerardi, Flaviana Gottardo, Severino Segato
Summary: The study aimed to assess the seasonal variation in VOCs in raw milk from three indoor feeding systems. VOC profiles were determined by GC after HS-SPME. The results showed that the feeding system did not affect VOCs, but the season had a significant impact. The outcomes suggested that VOCs could be used to trace raw milk for further mild processing.
Article
Veterinary Sciences
Andrea Massaro, Alessandra Tata, Ivana Pallante, Valentina Bertazzo, Massimo Bottazzari, Laura Paganini, Brunella Dall'Ava, Annalisa Stefani, Jeroen De Buck, Roberto Piro, Nicola Pozzato
Summary: This study investigated the metabolic changes associated with natural MAP infection in dairy cattle using NMR spectroscopy and routine blood chemistry. The results showed altered metabolic pathways, including increased tyrosine metabolism and enhanced phenylalanine, tyrosine, and tryptophan biosynthesis in MAP-infected cattle. Increased synthesis and degradation of ketone bodies were observed in both infected and infectious cattle. The fusion of multiple data sources proved to be useful in exploring these metabolic pathway changes and potentially diagnosing negative animals within paratuberculosis-infected herds.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Agriculture, Multidisciplinary
F. Marzoli, A. Tata, A. Massaro, M. Bragolusi, E. Passabi, S. Cappellozza, A. Saviane, L. Tassoni, R. Piro, S. Belluco
Summary: Silk farming in Italy has declined in the past due to competition with Asian markets and pesticide use, but it has potential for future development in the food sector. This study aimed to assess the nutritional composition and presence of hazards in silkworms. The study found that some samples did not meet the microbiological criteria for certain bacteria and fungi. Additionally, traces of fungicides and heavy metals were observed, and mycotoxins were detected in certain products. Overall, this study highlights the potential impact of environmental contamination on the safety of silkworms and their derivative products.
JOURNAL OF INSECTS AS FOOD AND FEED
(2023)
Article
Food Science & Technology
Filippo Marzoli, Alessandra Tata, Carmela Zacometti, Serena Malabusini, Costanza Jucker, Roberto Piro, Antonia Ricci, Simone Belluco
Summary: This study evaluated the microbiological and chemical stability of A. domesticus powders during one year of storage at room temperature. The results showed that the cricket powders had suitable microbiological stability, but exhibited a clear tendency toward rancidity in terms of chemical stability. Samples dried at 120°C and lyophilized never exceeded the limit set for B. cereus count in A. domesticus powder. Lyophilized samples also had the lowest levels of aldehydes responsible for rancid smell.
FRONTIERS IN SUSTAINABLE FOOD SYSTEMS
(2023)
Article
Chemistry, Analytical
Carmela Zacometti, Alessandra Tata, Roberto Stella, Stefania Leone, Ivana Pallante, Marianna Merenda, Salvatore Catania, Nicola Pozzato, Roberto Piro
Summary: In this study, ambient mass spectrometry (AMS) screening method was applied to analyze a suspected animal poisoning case. The results showed evidence of vegetable material intoxication and confirmed the presence of toxic substances using other analysis methods. This study demonstrates the potential of AMS to offer a rapid and efficient alternative for analyzing toxic substances in autopsy specimens from animals.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Salvador Sanchez-Vinces, Gustavo Henrique Bueno Duarte, Marcia Cristina Fernandes Messias, Caroline Fernanda Alves Gatinoni, Alex Ap. Rosini Silva, Pedro Henrique Godoy Sanches, Carlos Augusto Real Martinez, Andreia M. Porcari, Patricia de Oliveira Carvalho
Summary: This study analyzed lipid abundance in tumor tissue and found potential lipids that may be associated with poor response to neoadjuvant therapy in locally advanced rectal cancer patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Carmela Zacometti, Andrea Massaro, Tommaso di Gioia, Stephane Lefevre, Aline Fregiere-Salomon, Jean-Louis Lafeuille, Ingrid Fiordaliso Candalino, Michele Suman, Roberto Piro, Alessandra Tata
Summary: The study utilized TD-DART-HRMS technique to analyze authentic and adulterated samples of black pepper and dried oregano, demonstrating the utility of this technique in establishing spice authenticity. Merging different datasets allowed retrieval of more comprehensive information compared to sole TD-(+)DART-HRMS spectra.
JOURNAL OF MASS SPECTROMETRY
(2023)
Article
Chemistry, Multidisciplinary
Pedro H. G. Sanches, Danilo C. de Oliveira, Ivan G. M. dos Reis, Anna M. A. P. Fernandes, Alex A. R. Silva, Marcos N. Eberlin, Patricia O. Carvalho, Gustavo H. B. Duarte, Andreia M. Porcari
Summary: This paper describes the development and application of a software named MoNA to Progenesis QI Library Converter, which allows Progenesis QI to identify metabolites in metabolomics experiments. By correcting fragmentation data, this software enables the compatibility between PQI and MoNA. The study suggests that converting library files to .msp format is necessary for improving the identification level of metabolites.
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
(2023)
