Review
Biochemical Research Methods
Lanaia Itala Louzeiro Maciel, Ricardo Alves Bernardo, Rafael Oliveira Martins, Almir Custodio Batista Jr, Joao Victor Ataide Oliveira, Andrea Rodrigues Chaves, Boniek Gontijo Vaz
Summary: The imaging of biological tissues using mass spectrometry imaging (MSI) techniques allows for the visualization of the distribution of various metabolites, drugs, lipids, and glycans in complex samples. MSI methods like DESI-MSI and MALDI-MSI provide high sensitivity and the ability to evaluate/visualize multiple analytes in a single sample, overcoming limitations of classical microscopy techniques. This review discusses the application of DESI-MSI and MALDI-MSI in evaluating exogenous and endogenous molecules in biological samples, providing technical insights and recent research findings.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Ouyang Dan, Chuanzhe Wang, Chao Zhong, Juan Lin, Gang Xu, Guane Wang, Zian Lin
Summary: In this study, novel LDI-MS substrates were designed to enhance the sensitivity of mass signals and achieve high accuracy diagnosis of different saccharides and central precocious puberty (CPP).
Review
Biochemical Research Methods
Jing Wang, Madison. E. E. Pursell, Amanda Devor, Olanrewaju Awoyemi, Stephen. J. J. Valentine, Peng Li
Summary: This review discusses the basics, recent advancements, and application areas of portable mass spectrometers. Developing portable mass spectrometers can expand the application scope and user groups of mass spectrometry analysis and bring potential benefits to 'omics analysis.
Article
Chemistry, Analytical
Frederic Fournelle, Nidia Lauzon, Ethan Yang, Pierre Chaurand
Summary: Traditionally, MALDI IMS requires the deposition of an organic matrix, which often generates high background noise in the low mass range. LDI offers selective ionization with low background in the low mass range. This article discusses the advantages and disadvantages of solvent free and wet deposition of metals as LDI matrices and demonstrates the power of metal-assisted LDI for analyzing low molecular weight molecules in tissue sections.
MICROCHEMICAL JOURNAL
(2023)
Review
Materials Science, Multidisciplinary
Zhe Yang, Zhengyi Ren, Yongjun Cheng, Wenjun Sun, Zhenghua Xi, Wenjie Jia, Gang Li, Yongjun Wang, Meiru Guo, Detian Li
Summary: This article presents a review of the miniaturization process and application of four types of portable mass spectrometers, along with an introduction to suitable ion sources and detectors. The research of portable mass spectrometers is discussed to provide recommendations for future designs.
Article
Chemistry, Analytical
Yuanxia Lv, Xiaohuan Qin, Kun Hu, Yong Huang, Shulin Zhao
Summary: The novel matrix-assisted laser desorption/ionization time-of-flight mass spectrometric method developed based on MoS2/g-C3N4 shows enhanced UV absorption and improved desorption and ionization efficiency for highly sensitive small molecular compounds detection. It also exhibits low detection limits, good reproducibility, and capability to directly determine uric acid in complex biological samples.
Article
Multidisciplinary Sciences
Hua Zhang, Lin Zhao, Jingjing Jiang, Jie Zheng, Li Yang, Yanyan Li, Jian Zhou, Tianshu Liu, Jianmin Xu, Wenhui Lou, Weige Yang, Lijie Tan, Weiren Liu, Yiyi Yu, Meiling Ji, Yaolin Xu, Yan Lu, Xiaomu Li, Zhen Liu, Rong Tian, Cheng Hu, Shumang Zhang, Qinsheng Hu, Yangdong Deng, Hao Ying, Sheng Zhong, Xingdong Zhang, Yunbing Wang, Hua Wang, Jingwei Bai, Xiaoying Li, Xiangfeng Duan
Summary: As cancer is increasingly seen as a metabolic disorder, serum metabolite profiling is proposed as a viable method for detecting cancer. In this study, a machine learning model using mass spectrometry-based liquid biopsy data is reported for pan-cancer screening and classification. The Multiplexed Nanomaterial-Assisted LDI for Cancer Identification (MNALCI) achieves high sensitivity and specificity for distinguishing cancers from healthy controls and accurately identifying the tissue of origin of tumors. This high throughput assay shows promising potential for non-invasive cancer diagnosis.
NATURE COMMUNICATIONS
(2022)
Article
Physics, Atomic, Molecular & Chemical
Yi-Hsin Chen, Chia-Yu Liu, Zachary J. Devereaux, Chia-Yun Wang, Sarah Trimpin, I-Chung Lu
Summary: Mass spectrometry has the potential to provide faster and more accurate analyses for diagnosing diseases than current diagnostics and point-of-care technologies. A portable mass spectrometer using a homebuilt ionization source based on matrix-assisted ionization (MAI) was developed for clinical measurements. This portable MAI-MS allows for both qualitative and quantitative analysis of clinically-relevant samples with user-friendly sample introduction.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2022)
Review
Materials Science, Multidisciplinary
Chen-yu Wang, Kai Jiao, Jian-fei Yan, Mei-chen Wan, Qian-qian Wan, Lorenzo Breschi, Ji-hua Chen, Franklin R. Tay, Li-na Niu
Summary: This review provides an overview of the state-of-the-art research in biomineralized inorganic and hybrid materials prepared using biological or synthetic templates. These materials have applications in biomedical, physical, chemical, and environmental fields, and their synthesis, properties, and progress are discussed to guide future research.
PROGRESS IN MATERIALS SCIENCE
(2021)
Review
Pharmacology & Pharmacy
Manman Qin, Yuqing Qian, Lu Huang, Chao Zhong, Mingdong Li, Jun Yu, Huanwen Chen
Summary: Extraction electrospray ionization mass spectrometry (EESI-MS) enables effective ionization of trace molecules in complex samples without sample pretreatment, providing high sensitivity, selectivity, and response speed. It also allows for advanced functionalization of high-energy molecular species. This article systematically reviews the effects of operating conditions on the analytical performance of EESI-MS for various pharmaceutical compounds and introduces its potential application in molecular functionalization for drug discovery.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Chemistry, Analytical
Ge Ma, Xiaoyong Zhao, Mengzhe Guo, Yaqin Liu, Keqiang Shi, Cheng Guo, Yuanjiang Pan
Summary: In this study, a matrix glycosylation strategy was proposed to improve the sensitivity and signal reproducibility of MALDI MS analysis. The glycosylated matrices, especially 6-GAQ, exhibited remarkably-improved performance in detecting metabolites and imaging small molecules in complex samples. The glycosylation strategy showed great potential in the field of mass spectrometry.
ANALYTICA CHIMICA ACTA
(2022)
Article
Multidisciplinary Sciences
Margot De Spiegeleer, Vera Plekhova, Jozefien Geltmeyer, Ella Schoolaert, Beata Pomian, Varoon Singh, Kathleen Wijnant, Kimberly De Windt, Volter Paukku, Alexander De Loof, Inge Gies, Nathalie Michels, Stefaan De Henauw, Marilyn De Graeve, Karen De Clerck, Lynn Vanhaecke
Summary: In recent years, ambient ionization mass spectrometry (AIMS) has allowed for direct analysis of biofluid metabolome. However, AIMS procedures are hindered by analytical and practical limitations, which affect metabolome coverage. This study aimed to develop biofluid-specific metabolome sampling membranes (MetaSAMPs) to improve AIMS. Customized MetaSAMPs made of hydrophilic and lipophilic polymers demonstrated superior metabolome coverage and transport stability compared to crude biofluid analysis. This new approach was successfully validated and has potential applications in metabolic health stratification.
Article
Biochemical Research Methods
Cristina Arciniega, Kenneth P. Garrard, Jacob P. Guymon, Jeffrey G. Manni Sr, Alex Apffel, John C. Fjeldsted, David C. Muddiman
Summary: High-throughput screening (HTS) is a technique used by pharmaceutical companies to quickly screen multiple compound libraries for potential drugs. Mass spectrometry (MS) is a popular choice for HTS because it can analyze hundreds to thousands of compounds simultaneously without additional chemical derivatization. In this study, we developed an infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) source coupled directly to an Agilent 6545 mass spectrometer for direct analysis from well plates. The source used a laser operating at 100 Hz with high ionization efficiency, and a metal capillary was constructed to improve ion transmission and sensitivity.
JOURNAL OF MASS SPECTROMETRY
(2023)
Article
Chemistry, Multidisciplinary
Yanmei Yang, Weihua Niu, Weiqing Wang, Siyun Qi, Lili Tong, Xiaoyan Mu, Zhenzhen Chen, Weifeng Li, Bo Tang
Summary: The introduction of fluorinated boron nitride nanosheet (h-FBN) greatly improves the detection sensitivity and signal stability in negative ion mode paper spray mass spectrometry (PS-MS).
CHEMICAL COMMUNICATIONS
(2021)
Article
Plant Sciences
Yukun Liu, Xiaofei Nie, Jilong Wang, Zhenqi Zhao, Zhimei Wang, Fang Ju
Summary: Flavonoids in litchi seeds have broad-spectrum antiviral and antitumor activities, especially against breast and liver cancers. MALDI-MSI was used to detect and image the distribution of flavonoids in litchi seed tissue sections for the first time, revealing their heterogeneous distribution and potential for extraction.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Analytical
Zheng Ao, Hongwei Cai, Zhuhao Wu, Jonathan Krzesniak, Chunhui Tian, Yvonne Y. Lai, Ken Mackie, Feng Guo
Summary: The study introduces a human spinal organoid-on-a-chip device for modeling the biology and electrophysiology of human nociceptive neurons and dorsal horn interneurons. The device allows for testing nociceptive modulators and is cost-efficient, scalable, and easy to use. By integrating human sensory-spinal-cord organoids, the method shows promise for screening and validating novel therapeutics for human pain medicine discovery.
ANALYTICAL CHEMISTRY
(2022)
Review
Pharmacology & Pharmacy
Ming Tatt Lee, Ken Mackie, Lih-Chu Chiou
Summary: This review explores the potential and contributions of activating the endocannabinoid system and peripheral neuromodulation in opioid tolerance.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Lawrence M. Carey, Zhili Xu, Gabriela Rajic, Alexandros Makriyannis, Julian Romero, Cecilia Hillard, Ken Mackie, Andrea G. Hohmann
Summary: This study suggests that CB2 activation may alleviate HIV-associated antiretroviral neuropathy and identifies a previously unreported mechanism through which CB2 activation produces antinociceptive efficacy. The study also demonstrates that a CB2 agonist can reverse established morphine tolerance and shows that CB2 localized to peripheral sensory neurons mediates the opioid tolerance sparing efficacy of CB2 agonists.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Chemistry, Medicinal
Toni Kline, Cong Xu, Faith R. Kreitzer, Dow P. Hurst, Khalil M. Eldeeb, Jim Wager-Miller, Kathleen Olivas, Seon A. Hepburn, John W. Huffman, Ken Mackie, Allyn C. Howlett, Patricia Reggio, Nephi Stella
Summary: The author synthesized fourteen novel alkylindole analogues and evaluated their activities at alkylindole-sensitive GPCRs. They found three characteristics that favor binding to alkylindole-sensitive GPCRs versus CB1R/CB2R and identified novel chemical tools for studying alkylindole-sensitive GPCRs.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Toxicology
Jinhwan Lim, Hye-Lim Lee, Julie Nguyen, Joyce Shin, Samantha Getze, Caitlin Quach, Erica Squire, Kwang-Mook Jung, Stephen Mahler, Ken Mackie, Daniele Piomelli, Ulrike Luderer
Summary: Adolescent exposure to THC may cause aberrant activation of the ovarian endocannabinoid system in female mice, resulting in substantial loss of ovarian reserve in adulthood. Further investigation is needed to understand the relevance of these findings to women who frequently used cannabis during adolescence.
TOXICOLOGICAL SCIENCES
(2023)
Article
Engineering, Biomedical
Hongwei Cai, Zheng Ao, Chunhui Tian, Zhuhao Wu, Connor Kaurich, Zi Chen, Mingxia Gu, Andrea G. Hohmann, Ken Mackie, Feng Guo
Summary: Opioids may induce tolerance and hyperalgesia, but the human mechanisms underlying these effects are not well understood. Researchers have developed a novel human spinal microphysiological system (MPS) to model human nociception and opioid-induced tolerance. This system shows promising potential for studying pain etiology, screening new treatments, and validating novel therapeutics for human pain medicine.
BIOACTIVE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Jonah E. Zarrow, Abdul-Musawwir Alli-Oluwafuyi, Cristina M. Youwakim, Kwangho Kim, Andrew N. Jenkins, Isabelle C. Suero, Margaret R. Jones, Zahra Mashhadi, Ken Mackie, Alex G. Waterson, Amanda C. Doran, Gary A. Sulikowski, Sean S. Davies
Summary: N-Acyl-phosphatidylethanolamine hydrolyzing phospholipase D (NAPE-PLD) is an enzyme that hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce N-acyl-ethanolamines (NAEs) and phosphatidic acid. Reduced NAPE-PLD activity is associated with cardiometabolic diseases, and small molecule activators of NAPE-PLD could be a potential therapeutic treatment. A series of benzothiazole phenylsulfonyl-piperidine carboxamides were identified as NAPE-PLD activators, which increased efferocytosis by macrophages. These findings suggest the importance of NAPE-PLD in regulating efferocytosis and the potential use of NAPE-PLD activators for treating cardiometabolic diseases.
ACS CHEMICAL BIOLOGY
(2023)
Article
Neurosciences
Kelsey G. Guenther, Zhili Xu, Julian Romero, Cecilia J. Hillard, Ken Mackie, Andrea G. Hohmann
Summary: CB2 agonists can suppress pathological pain in animal models without unwanted side effects associated with CB1 receptor activation. However, the specific types of pain responsive to CB2 agonists and the cell types underlying therapeutic efficacy are not fully understood.
Article
Pharmacology & Pharmacy
Axel Bernabeu, Anissa Bara, Michelle N. Murphy Green, Antonia Manduca, Jim Wager-Miller, Milene Borsoi, Olivier Lassalle, Anne-Laure Pelissier-Alicot, Pascale Chavis, Ken Mackie, Olivier J. J. Manzoni
Summary: This study reveals sex differences in the maturational trajectories of the rat prefrontal cortex (PFC). The excitability of PFC neurons is lower in adult females compared to other developmental stages. The gene expression of the endocannabinoid/vanilloid systems is sequential and sex-specific.
CANNABIS AND CANNABINOID RESEARCH
(2023)
Correction
Clinical Neurology
Mei Hu, Dexiao Zhu, Jian Zhang, Fei Gao, Jack Hashem, Philip Kingsley, Lawrence J. Marnett, Ken Mackie, Chu Chen
Article
Pharmacology & Pharmacy
Pedro Henrique Gobira, Jacob LaMar, Jade Marques, Ariandra Sartim, Kennia Silveira, Luana Santos, Gregers Wegener, Francisco S. Guimaraes, Ken Mackie, Hui-Chen Lu, Samia Joca
Summary: This study investigates the involvement of cannabinoid type 1 receptors (CB1R) in the antidepressant and psychostimulant effects induced by ketamine. The results suggest that CB1R mediate the psychostimulant side effects of ketamine, but do not play a role in its antidepressant properties. Furthermore, pharmacological blockade of CB1R has similar antidepressant effects, but does not potentiate the effects of ketamine.
CANNABIS AND CANNABINOID RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Joanna Winstone, Hana Shafique, Madeleine E. Clemmer, Ken Mackie, Jim Wager-Miller
Summary: This study found changes in BDNF mRNA expression in the hippocampus of adolescent mice exposed to THC and CBD. THC represses transcript expression for some BDNF variants, and this effect is rescued when CBD is co-administered. Sex differences were observed in specific BDNF isoforms.
CANNABIS AND CANNABINOID RESEARCH
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
Joshua L. Rein, Ken Mackie, Thomas R. Kleyman, Lisa M. Satlin
Article
Clinical Neurology
Mei Hu, Dexiao Zhu, Jian Zhang, Fei Gao, Jack Hashem, Philip Kingsley, Lawrence J. Marnett, Ken Mackie, Chu Chen
Summary: Traumatic brain injury is a risk factor for Alzheimer's disease and dementia. In this study, the researchers found that inhibiting monoacylglycerol lipase can reduce neuropathology caused by traumatic brain injury. The neuroprotective effects are mainly due to the inhibition of 2-arachidonoylglycerol metabolism in astrocytes, and not in neurons.
Article
Pharmacology & Pharmacy
Izaque de Sousa Maciel, Gabriel H. D. de Abreu, Claire T. Johnson, Rida Bonday, Heather B. Bradshaw, Ken Mackie, Hui-Chen Lu
Summary: The study using mice found that perinatal cannabinoid exposure affects behaviors in a sex-dependent manner, altering repetitive and hedonic behaviors. Additionally, perinatal exposure to THC or CBD prevents fluoxetine from enhancing coping behavior.
CANNABIS AND CANNABINOID RESEARCH
(2022)