Article
Biochemistry & Molecular Biology
Johny M. Nguyen, Rebecca E. Moore, Sabrina K. Spicer, Jennifer A. Gaddy, Steven D. Townsend
Summary: The study found that a zwitterionic cellulose derivative in Escherichia coli plays an important role in biofilm formation and cellular adhesion. Furthermore, a synthetic zwitterionic phosphoethanolamine cellobiose also showed enhanced biofilm activity in the gram-negative bacterium E. coli. This research provides new opportunities to study the impact glycopolymers have on cellular adhesion in gram-negative pathogens.
Article
Microbiology
Xianqin Yang, Frances Tran, Peipei Zhang
Summary: The phylogeny and genetic elements of biofilm forming and nonbiofilm forming E. coli strains from a beef processing environment were investigated. The majority of both groups belonged to phylogroup B1. Comparative genomic analysis revealed a tree-wide spread of biofilm forming strains, with no overrepresented genes or mutations. There was a correlation between biofilm formation and curli production, cellulose synthesis, and motility, but some strains lacked these traits. Additional substrate utilization and iron acquisition were found in the persisting E. coli group.
MICROBIOLOGY SPECTRUM
(2023)
Article
Nutrition & Dietetics
Mohd W. W. Azam, Asad U. U. Khan
Summary: This study demonstrates that knocking down the csgD gene can reduce amyloid and biofilm formation, as well as suppress the expression of genes associated with pathogenicity.
FRONTIERS IN NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
Kotaro Sano, Hiroaki Kobayashi, Hirotaka Chuta, Nozomi Matsuyoshi, Yuki Kato, Hiroshi Ogasawara
Summary: The YccT gene, regulated by CsgD, has been found to inhibit curli fimbriae formation in bacteria. Overexpression of CsgD also strongly represses curli fimbriae formation. By deleting the N-terminal signal peptide of YccT, it was found that the inhibitory effect of YccT is mediated by the two-component regulatory system EnvZ/OmpR.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
R. Kavi Bharathi, C. S. Srinandan, N. Sai Subramanian
Summary: This study investigates the cooperative ability and matrix sharing between specialist and generalist strains during biofilm formation. The results show that the matrix aids in survival during antibiotic, chlorine, and predatory stress, and possible sharing of the matrix occurs in co-culture.
Article
Biochemistry & Molecular Biology
Jamie Jeffries, Wiriya Thongsomboon, Joshua Alan Visser, Kyle Enriquez, Deborah Yager, Lynette Cegelski
Summary: Bacterial biofilms are intricate communities of bacteria that utilize insoluble biopolymers like curli amyloid fibers and phosphoethanolamine (pEtN) cellulose to construct unique biofilm architectures. This study compared the composition and biofilm phenotypes of two different E. coli strains, revealing variability in matrix assembly principles among strains. Results suggest that curli primarily affect adhesion, while pEtN cellulose drives cohesion in biofilms, with their influence depending on the biofilm modality and the strain itself.
Article
Microbiology
Bo Hou, Chen-Yan Wang, Shao-Wen Li, Lun-Jiang Zhou, Yong-Liang Che, Qiu-Yong Chen
Summary: The HicAB type II T-A system is abundant in various bacteria and archaea and plays important roles in stress response, virulence, and bacterial persistence. This study demonstrated that the deletion of hicAB significantly decreased the biofilm-forming ability of ExPEC, but the mutant still retained the ability to form curli fimbriae. Further analysis revealed differential gene expression associated with biofilm formation between the hicAB mutant and the parent strain, suggesting that the HicAB system is involved in ExPEC biofilm formation through regulation of outer membrane protein gene expression.
CURRENT MICROBIOLOGY
(2023)
Article
Microbiology
Md. Manjurul Haque, Md Khaled Mosharaf, Md. Amdadul Haque, Md. Zahid Hasan Tanvir, Md. Khairul Alam
Summary: Bacterial strains of Enterobacter, Vitreoscilla, Acinetobacter, and Bacillus have been found to form different types of biofilms in response to varying environmental and nutritional conditions. These biofilms play a crucial role in metal biosorption from wastewater, with certain strains showing greater efficiency in the removal of copper, nickel, and lead. Fourier transform infrared spectroscopy results suggest that these bacterial biofilms contain functional groups capable of biosorbing metals, making them potential candidates for bioremediation in aquatic environments.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Engineering, Environmental
Martin Esteban Gonzalez-Lopez, Cesar Mario Laureano-Anzaldo, Aida Alejandra Perez-Fonseca, Cesar Gomez, Jorge Ramon Robledo-Ortiz
Summary: Cellulose beads loaded with graphene nanoplatelets were prepared using a physical gelation method and tested for the removal of Congo red dye. The adsorption equilibrium was described by Langmuir isotherm, with enhanced mass transfer kinetics using a differential column batch reactor. The presence of graphene nanoplatelets promoted faster kinetics and close to 100% adsorption efficiencies were achieved within a reasonably low time frame.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Zhaohui S. Xu, Tongbo Zhu, Zhiying Wang, Xianqin Yang, Michael G. Ganzle
Summary: This study investigated the genetic mechanisms and gene regulation of Escherichia coli in the formation of pellicles in different environments. The research revealed gene differences and expression level changes related to pellicle formation, providing important insights into the mechanisms of pellicle formation in E. coli and related organisms.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Chemistry, Applied
Yao Hui, Rukuan Liu, Liuzemu Li, Qingqing Sun, Zhihong Xiao, Airong Xu, Sisi Liu
Summary: Novel magnetic HC/Fe3O4 spherical materials were synthesized by crosslinking hydroxyethyl cellulose (HC) with poly(ethylene glycol) diglycidyl ether (PGDE), followed by the introduction of magnetic Fe3O4. The morphology, structure, magnetic behavior, point of zero-charge (pH(z)pc), and Brunauer-Emmet-Teller (BET) of the spherical materials were systematically investigated. The materials displayed a high adsorption capacity for removing dye from wastewater and could be easily recovered due to their magnetic property.
JOURNAL OF POROUS MATERIALS
(2023)
Article
Multidisciplinary Sciences
Anqi Cai, Zahra Abdali, Dalia Jane Saldanha, Masoud Aminzare, Noemie-Manuelle Dorval Courchesne
Summary: In order to address the environmental impact of the textile industry, self-repairing textile composites have been developed by integrating Escherichia coli biofilms into conventional textiles. The curli fibers in the biofilm have demonstrated the ability to self-assemble and self-heal. Through simple and scalable methods, such as adsorption, doctor blading, and vacuum filtration, the biofilms were successfully integrated into the textiles.
SCIENTIFIC REPORTS
(2023)
Article
Biophysics
Esra Yuca, Ebru Sahin Kehribar, Urartu Ozgur Safak Seker
Summary: The study revealed that CsgA and CsgB exhibit varying binding strengths on different surfaces, with CsgB being crucial for determining the adhesiveness of the CsgA-CsgB mixture on hydroxyapatite and silica surfaces, and CsgA controlling the behavior of the mixture on gold surfaces. These findings offer insights into the binding behavior of curli proteins on different surfaces, aiding in achieving more precise control over their adhesion to targeted surfaces.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Polymer Science
Chenlu Jiao, Die Liu, Nana Wei, Jiannan Gao, Fan Fu, Tao Liu, Jian Wang
Summary: Porous sustainable cellulose/gelatin/sepiolite gel beads were successfully fabricated for CR removal from wastewater, demonstrating a highly porous and thermally stable structure. The adsorption capacity for CR reached 279.3 mg·g(-1) with good regeneration capability. These gel beads show potential as efficient and reusable materials for dye wastewater treatments.
Article
Polymer Science
Uroosa Ejaz, Agha Arslan Wasim, Muhammad Nasiruddin Khan, Othman M. Alzahrani, Samy F. Mahmoud, Zeinhom M. El-Bahy, Muhammad Sohail
Summary: This study utilized fermented residue sugarcane bagasse as an adsorbent for removing toxic dyes from industrial wastewater. By pretreating and fermenting the bagasse, the efficiency of dye removal was improved. Results showed that the treated bagasse had good adsorption performance and high cellulase production.
Article
Multidisciplinary Sciences
Wiriya Thongsomboon, Diego O. Serra, Alexandra Possling, Chris Hadjineophytou, Regine Hengge, Lynette Cegelski
Article
Multidisciplinary Sciences
Courtney Reichhardt, Lynette Cegelski
Article
Multidisciplinary Sciences
Emily C. Hollenbeck, Alexandra Antonoplis, Chew Chai, Wiriya Thongsomboon, Gerald G. Fuller, Lynette Cegelski
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Review
Infectious Diseases
Courtney Reichhardt, Lydia-Marie Joubert, Karl V. Clemons, David A. Stevens, Lynette Cegelski
Article
Microbiology
David Zamorano-Sanchez, Wujing Xian, Calvin K. Lee, Mauro Salinas, Wiriya Thongsomboon, Lynette Cegelski, Gerard C. L. Wong, Fitnat H. Yildiz
Review
Microbiology
Courtney Reichhardt, Matthew R. Parsek
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Multidisciplinary Sciences
Daniel Passos da Silva, Michael L. Matwichuk, Delaney O. Townsend, Courtney Reichhardt, Doriano Lamba, Daniel J. Wozniak, Matthew R. Parsek
NATURE COMMUNICATIONS
(2019)
Article
Microbiology
Wiriya Thongsomboon, Sabrina H. Werby, Lynette Cegelski
JOURNAL OF BACTERIOLOGY
(2020)
Article
Microbiology
Courtney Reichhardt, Holly M. Jacobs, Michael Matwichuk, Cynthis Wong, Daniel J. Wozniak, Matthew R. Parsek
JOURNAL OF BACTERIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Jamie Jeffries, Wiriya Thongsomboon, Joshua Alan Visser, Kyle Enriquez, Deborah Yager, Lynette Cegelski
Summary: Bacterial biofilms are intricate communities of bacteria that utilize insoluble biopolymers like curli amyloid fibers and phosphoethanolamine (pEtN) cellulose to construct unique biofilm architectures. This study compared the composition and biofilm phenotypes of two different E. coli strains, revealing variability in matrix assembly principles among strains. Results suggest that curli primarily affect adhesion, while pEtN cellulose drives cohesion in biofilms, with their influence depending on the biofilm modality and the strain itself.
Correction
Microbiology
David Zamorano-Sanchez, Wujing Xian, Calvin K. Lee, Mauro Salinas, Wiriya Thongsomboon, Lynette Cegelski, Gerard C. L. Wong, Fitnat H. Yildiz
Article
Chemistry, Medicinal
Elaine Limqueco, Daniel Passos Da Silva, Courtney Reichhardt, Fang-Yi Su, Debobrato Das, Jasmin Chen, Selvi Srinivasan, Anthony Convertine, Shawn J. Skerrett, Matthew R. Parsek, Patrick S. Stayton, Daniel M. Ratner
ACS INFECTIOUS DISEASES
(2020)
Article
Cell Biology
Laura K. Jennings, Julia E. Dreifus, Courtney Reichhardt, Kelly M. Storek, Patrick R. Secor, Daniel J. Wozniak, Katherine B. Hisert, Matthew R. Parsek
Summary: In cystic fibrosis airways, Pseudomonas aeruginosa forms biofilms that are thought to contribute to chronic infection. Exopolysaccharides Pel and Psi are expressed in CF airways and play a role in the aggregation mechanism, potentially impacting therapies by increasing antimicrobial tolerance and protecting extracellular DNA from digestion.
Review
Environmental Sciences
Yeonsoo Park, Mina Solhtalab, Wiriya Thongsomboon, Ludmilla Aristilde
Summary: Soil bacteria employ various strategies to overcome the limitation of inorganic phosphorus in soils by secreting extracellular enzymes to degrade organic phosphorus and utilizing specific transporters and intracellular metabolic pathways for phosphorus recycling. The activities of phosphatases are influenced by soil environmental conditions. Regulatory mechanisms play a crucial role in the production of phosphatases, expression of transporters, and metabolic changes in response to phosphorus availability.
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2022)
Review
Chemistry, Multidisciplinary
Kristen Amyx-Sherer, Courtney Reichhardt
Summary: Biofilm formation protects bacteria, making it difficult to treat infections. However, studying biofilm matrix components, especially exopolysaccharides, has been challenging due to their complex nature. Solid-state NMR has emerged as a key tool to study these materials.
MAGNETIC RESONANCE IN CHEMISTRY
(2023)