Article
Developmental Biology
Sara A. Miller, Manashree Damle, Jongmin Kim, Robert E. Kingston
Summary: The study showed that full methylation of H3K27 was not necessary for the formation of differentiated cell states during embryoid body formation, but was required to maintain a stable differentiated state.
Article
Biochemistry & Molecular Biology
Shuang Zhang, Jeroen van de Peppel, Marijke Koedam, Johannes P. T. M. van Leeuwen, Bram C. J. van der Eerden
Summary: In this study, TNS3 was found to be involved in the fate decision of human bone marrow-derived stromal cells (BMSCs), regulating osteogenesis and adipogenesis through the control of RhoA activity and cytoskeletal reorganization. The findings highlight the crucial role of TNS3 in bone metabolism.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Chong Zeng, Zhongbao Shao, Zibo Wei, Jie Yao, Weidong Wang, Liang Yin, Huixian YangOu, Dan Xiong
Summary: This study indicates the regulatory role of NOTCH signaling in Th22 cell differentiation, highlighting the importance of HES-1 in the upregulation of AHR and IL-22. Enhancing HES-1 through NOTCH signaling promotes naive CD4(+) T cell skewing towards Th22 cells.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2021)
Article
Oncology
Marco De Martino, Pedro Nicolau-Neto, Luis Felipe Ribeiro Pinto, Alexandra Traverse-Glehen, Emmanuel Bachy, Vincenzo Gigantino, Rossella De Cecio, Francesco Bertoni, Paolo Chieffi, Alfredo Fusco, Francesco Esposito
Summary: The study found a significant correlation between HMGA1 and EZH2 expression in human lymphomas, where HMGA1 was able to bind the EZH2 promoter and induce its activity. Silencing HMGA1 expression resulted in decreased EZH2 levels, leading to reduced proliferation and migration rates of human lymphoma cell lines. These findings identify HMGA1 as an activator of EZH2, suggesting a novel molecular mechanism contributing to EZH2 overexpression in human malignancies and a synergism between the two proteins in cancer progression.
AMERICAN JOURNAL OF CANCER RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Ying Zhang, Elena V. Knatko, Maureen Higgins, Sharadha Dayalan Naidu, Gillian Smith, Tadashi Honda, Laureano de la Vega, Albena T. Dinkova-Kostova
Summary: The overexpression of pirin in colorectal cancer is correlated with the activation of Nrf2 and increased expression of NQO1, but not with the expression of AKR1B10 and AKR1C1. Pirin is regulated by Nrf2 and its inhibition can decrease the viability of colorectal cancer cells.
Review
Oncology
Liu Zhaoyun, Jia Yue, Guo Yixuan, Wang Hao, Fu Rong
Summary: In this paper, we reviewed the role of EZH2 in regulating bone marrow mesenchymal stem cell differentiation and immune cell function in the tumor microenvironment, summarized existing EZH2 inhibitors and main clinical trials, and proposed relevant ideas for potential clinical applications.
CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zeng Yang, Bo Wei, Anbang Qiao, Popo Yang, Wenhui Chen, Dezhi Zhen, Xiaojian Qiu
Summary: This study discovered the high expression of NXPH4 in non-small cell lung cancer and confirmed that knocking down NXPH4 can inhibit cell proliferation and migration, causing cell cycle arrest in the S1 phase. Moreover, NXPH4 was found to be regulated by the transcriptional activation of EZH2 and interacted with CDKN2A to participate in cell cycle regulation.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Gabriella P. A. de Freitas, Luiz Henrique M. Geraldo, Bruna M. Faria, Soniza Vieira Alves-Leon, Jorge Marcondes de Souza, Vivaldo Moura-Neto, Bruno Pontes, Luciana F. Romao, Patricia P. Garcez
Summary: Studies have revealed the overexpression of CENPJ in glioblastoma cell lines, showing its crucial role in cell proliferation and migration. Loss of function of CENPJ results in impairments in cell proliferation and migration, indicating its significant involvement in glioblastoma progression.
JOURNAL OF NEUROCHEMISTRY
(2022)
Article
Immunology
Anna K. Kania, Madeline J. Price, Lou-Ella George-Alexander, Dillon G. Patterson, Sakeenah L. Hicks, Christopher D. Scharer, Jeremy M. Boss
Summary: This study demonstrates that H3K27 demethylases inhibit B cell differentiation.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Immunology
Anna K. Kania, Madeline J. Price, Lou-Ella George-Alexander, Dillon G. Patterson, Sakeenah L. Hicks, Christopher D. Scharer, Jeremy M. Boss
Summary: In this study, it was found that the H3K27 demethylases restrain B cell differentiation. Mice with simultaneous genetic deletion of UTX and JMJD3 showed a significant increase in plasma cell formation and increased ATP synthesis capacity. Furthermore, UTX was found to regulate chromatin accessibility of known repressors of PC fate.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Lien Provez, Bart Van Puyvelde, Laura Corveleyn, Nina Demeulemeester, Sigrid Verhelst, Beatrice Lintermans, Simon Daled, Juliette Roels, Lieven Clement, Lennart Martens, Dieter Deforce, Pieter Van Vlierberghe, Maarten Dhaenens
Summary: Omics studies are ideal for generating hypotheses on health and disease. While epigenomics focuses mainly on DNA methylation using sequencing technologies, the study of histone posttranslational modifications (hPTMs) using mass spectrometry is lagging behind. This article presents a comprehensive mass spectrometry-based preprocessed hPTM atlas, providing a valuable resource for exploring histone analysis and epidrug development.
Article
Medicine, Research & Experimental
Tuan Anh Le, Van Trung Chu, Andreia C. Lino, Eva Schrezenmeier, Christopher Kressler, Dania Hamo, Klaus Rajewsky, Thomas Doerner, Van Duc Dang
Summary: Gene editing using CRISPR-Cas9 technology in human B cells can help identify gene regulatory networks and therapeutic targets for autoimmune diseases. Targeting IRF4, PRDM1, and XBP1 genes severely impairs B cell survival and plasma cell differentiation. This method provides a new avenue for studying gene functions and identifying plasma cell regulators.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Medicine, Research & Experimental
Fa Wang, Christopher Ting, Kent A. Riemondy, Michael Douglas, Kendall Foster, Nisha Patel, Norihito Kaku, Alexander Linsalata, Jean Nemzek, Brian M. Varisco, Erez Cohen, Jasmine A. Wilson, David W. H. Riches, Elizabeth F. Redente, Diana M. Toivola, Xiaofeng Zhou, Bethany B. Moore, Pierre A. Coulombe, M. Bishr Omary, Rachel L. Zemans
Summary: Idiopathic pulmonary fibrosis (IPF) is a progressive scarring disease caused by impaired regeneration of alveolar epithelial cells after injury. Genetic variants of human keratin 8 (KRT8) are associated with IPF. In mouse models, transitional cells and fibrosis resolve, while in human IPF, transitional AECs evolve into an aberrant basaloid state that persists with progressive fibrosis.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Endocrinology & Metabolism
Perla Cota, Lama Saber, Damla Taskin, Changying Jing, Aimee Bastidas-Ponce, Matthew Vanheusden, Alireza Shahryari, Michael Sterr, Ingo Burtscher, Mostafa Bakhti, Heiko Lickert
Summary: This study investigates the expression pattern and function of the NEUROD2 gene during human endocrinogenesis. The results suggest that NEUROD2 is not necessary for the formation of human b cells.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Engineering, Environmental
Meng Wu, Jing Sun, Li Wang, Peiwen Wang, Tian Xiao, Suhua Wang, Qizhan Liu
Summary: Arsenic compounds are widely distributed environmental toxins that can cause hepatic fibrosis and other damage when chronically exposed to low levels. This study found that arsenite promotes the differentiation of Th17 cells mediated by ROR gamma t in CD4+ T cells through down-regulation of HOTAIR and miR-17-5p. This leads to the secretion of IL-17A, which activates HSCs and facilitates hepatic fibrosis. These findings reveal a new mechanism and potential therapeutic target for arsenite-induced hepatic fibrosis.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Review
Cell Biology
Victoria Parreno, Anne-Marie Martinez, Giacomo Cavalli
Summary: Misregulated Polycomb proteins play a pleiotropic role in cancer, altering various biological processes crucial for tumor progression by modulating the expression of key genes in cellular decisions. Interfering with PcG functions can be a powerful strategy to counter tumor progression.
Article
Biochemistry & Molecular Biology
Amandine Barral, Gabrielle Pozo, Lucas Ducrot, Giorgio L. Papadopoulos, Sandrine Sauzet, Andrew J. Oldfield, Giacomo Cavalli, Jerome Dejardin
Summary: A study revealed the presence of dual regions with both H3K9me3 and H3K36me3 marks in mouse embryonic stem cells, which lose these marks and gain enhancer signatures upon removal of SETDB1, suggesting an important role for heterochromatin in gene control. In differentiated tissues, some of these dual domains become destabilized and enriched in enhancer marks, providing insight into heterochromatin involvement in cell identity maintenance.
Article
Oncology
Hatice Satilmis, Emma Verheye, Philip Vlummens, Inge Oudaert, Niels Vandewalle, Rong Fan, Jennifer M. Knight, Nathan De Beule, Gamze Ates, Ann Massie, Jerome Moreaux, Anke Maes, Elke De Bruyne, Karin Vanderkerken, Eline Menu, Erica K. Sloan, Kim De Veirman
Summary: This study investigates the potential therapeutic effects of beta-blockers, specifically targeting the beta(2)-adrenergic receptor, in multiple myeloma treatment. The blockade of beta(2)-adrenergic receptors reduces cell viability, induces apoptosis and autophagy, and modulates cancer cell metabolism. Combining beta(2)AR blockade with other drugs enhances apoptosis in multiple myeloma cells.
JOURNAL OF PATHOLOGY
(2023)
Meeting Abstract
Hematology
Valentin Jacquier, Antoine Guillemin, Sara Ovejero-Merino, Guilhem Requirand, Ouissem Karmous-Gadacha, Laure Dutrieux, Andrea Romero, Alizee Steer, Amelie Machura, Hugues De Boussac, Jerome Moreaux, Charles Herbaux
Article
Multidisciplinary Sciences
Tatyana G. Kahn, Mikhail Savitsky, Chikuan Kuong, Caroline Jacquier, Giacomo Cavalli, Jia-Ming Chang, Yuri B. Schwartz
Summary: Drosophila insulators are DNA elements that regulate gene expression by limiting chromatin contacts. By mapping chromatin contacts in Drosophila cells without key insulator proteins CTCF and Cp190, we discovered hundreds of insulator elements. Our findings suggest that Drosophila insulators play a minor role in overall genome folding but have a local impact on chromatin contacts. Our insulator catalog is an important resource for studying genome folding mechanisms.
Article
Multidisciplinary Sciences
Gonzalo Sabaris, Maximilian H. H. Fitz-James, Giacomo Cavalli
Summary: Increasing evidence suggests that environmental stress can trigger epigenetic signals that persist over long timeframes, leading to phenotypic changes and potential selection. Epigenetic inheritance plays a crucial role in rapid phenotypic adaptation to fluctuating environments, ensuring short-term survival while maintaining a bet-hedging strategy. These findings call for a reevaluation of the importance of nongenetic information in adaptive evolution and its broader relevance in nature.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
L. Baert, B. Manfroi, M. Quintero, O. Chavarria, P. V. Barbon, E. Clement, A. Zeller, T. Van Kuppevelt, N. Sturm, J. Moreaux, A. Tveita, B. Bogen, T. McKee, B. Huard
Summary: Multiple myeloma is a hematological neoplasm derived from plasma cells in the bone marrow. A subpopulation of cells called 10e4(+) cells, which have high resistance to multiple myeloma drugs, bind to the protein APRIL through heparan sulfate chains on syndecan-1. These cells have high proliferation activity and are able to form colonies in 3D cultures. They can develop in the bone marrow after intravenous injection and become more resistant to drugs after treatment. The enzyme HS3ST3a1 plays a role in modifying syndecan-1 to confer reactivity to 10e4 and APRIL binding. Targeting this enzyme could potentially improve drug resistance control in multiple myeloma.
Article
Genetics & Heredity
Mohammad Salma, Elina Alaterre, Jerome Moreaux, Eric Soler
Summary: Var vertical bar Decrypt is a web-based tool designed to extract relevant functional information from whole-exome sequencing (WES) data. It offers various filtering and analysis tools for prioritizing gene variants. By using Var vertical bar Decrypt, we successfully identified known disease oncogenes and novel putative drivers in WES datasets of acute erythroid leukemia patients.
EPIGENETICS & CHROMATIN
(2023)
Article
Hematology
Sarah Bonnet, Serge Carillo, Baptiste Legrand, Barbara Burroni, Thierry Lavabre-Bertrand, Guilhem Requirand, Nicolas Robert, Lea Fornero, Ahmed Al Mansoori, Jerome Moreaux, Guillaume Cartron, Ludovic Gabellier, Charles Herbaux
Summary: This case study describes a patient with extreme thrombocytosis who unfortunately had a rapidly fatal outcome. The cause of the thrombocytosis remained unknown and the patient did not show signs of myelofibrosis. However, dysplastic megakaryocytes were observed. The patient had a novel variant of the CALR gene in exon 3 (C105S), as well as mutations in ASXL1, U2AF1, and EZH2. These mutations were found in myeloid cells but not in lymphoid cells. The patient was diagnosed with a rare case of myelodysplastic syndrome/myeloproliferative neoplasm (MDS/MPN). Due to the high risk of thrombosis, the patient was treated with hydroxycarbamide. As the hematological status worsened, two new mutations, SETBP1 and ETV6, appeared, while the CALR mutation and the three other mutations identified in the chronic stage were still detectable. These findings suggest that the CALR variant may contribute to the pathogenesis of MDS/MPN in this patient.
EUROPEAN JOURNAL OF HAEMATOLOGY
(2023)
Letter
Oncology
Djamila Chemlal, Emmanuel Varlet, Amelie Machura, Sara Ovejero, Guilhem Requirand, Nicolas Robert, Guillaume Cartron, Elina Alaterre, Caroline Bret, Laure Vincent, Charles Herbaux, Giacomo Cavalli, Angelique Bruyer, Hugues De Boussac, Jerome Moreaux
Article
Medical Laboratory Technology
Emilia Boris, Alexandre Theron, Valentin Montagnon, Nicolas Rouquier, Marion Almeras, Jerome Moreaux, Caroline Bret
Summary: This study aimed to investigate the landscape of 7 leukemia-associated phenotype (LAP) markers in B acute lymphoblastic leukemia (B ALL). The expression levels of these markers in normal leukocytes, normal B cell differentiation, and B lymphoblasts at diagnosis of B ALL were evaluated. The study also examined the prognostic value of these markers using Maxstat R algorithm.
CYTOMETRY PART B-CLINICAL CYTOMETRY
(2023)
Meeting Abstract
Oncology
Catharina Muylaert, Lien Ann Van Hemelrijck, Elina Alaterre, Nicolas Robert, Guilhem Requirand, Kim De Veirman, Eline Menu, Karin Vanderkerken, Jerome Moreaux, Elke De Bruyne
CLINICAL LYMPHOMA MYELOMA & LEUKEMIA
(2023)
Meeting Abstract
Oncology
Inge Oudaert, Judith Lind, Osman Aksoy, Catharina Muylaert, Hatice Satilmis, Sylvia Faict, Jerome Moreaux, Kim De Veirman, Elke De Bruyne, Sonia Vallet, Karin Vanderkerken, Klaus Podar, Eline Menu
CLINICAL LYMPHOMA MYELOMA & LEUKEMIA
(2023)
Meeting Abstract
Oncology
Sara Ovejero, Julie Devin, Tatiana Caneque, Laura Henry, Laura Alibert, Guilhem Requirand, Nicolas Robert, Alizee Steer, Amelie Machura, Christophe Hirtz, Angelique Bruyer, Laure Vincent, Guillaume Cartron, Charles Herbaux, Raphael Rodriguez, Caroline Bret, Jerome Moreaux
CLINICAL LYMPHOMA MYELOMA & LEUKEMIA
(2023)
Meeting Abstract
Oncology
Laure Dutrieux, Guillemin Antoine, Lin Yea-Lih, Malik Lutzmann, Guilhem Requirand, Nicolas Robert, Laure, Guillaume Cartron, Charles Herbaux, Raphael Rodriguez, Michel Cogne, Philippe Pasero, Jerome Moreaux
CLINICAL LYMPHOMA MYELOMA & LEUKEMIA
(2023)