Article
Biochemistry & Molecular Biology
Marivee Borges-Rodriguez, Corbin A. Shields, Olivia K. Travis, Robert W. Tramel, Cedar H. Baik, Chelsea A. Giachelli, Jan MichaelWilliams, Geilda A. Tardo, Jan Michael Williams, Denise C. Cornelius
Summary: The study suggests that activated platelets may contribute to sepsis-induced renal injury through NLRP3 activation in platelets, indicating platelets could be a therapeutic target for reducing renal injury in septic patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Ayman Youssef, Bilal Khan Mohammed, Abhishek Prasad, Angela del Aguila, Gabriel Bassi, Wei Yang, Luis Ulloa
Summary: The recent discovery of TAK981, a selective inhibitor of SUMOylation, has the potential to revolutionize immune treatments. Clinical trials have shown its efficacy in metastatic tumors and hematologic malignancies. The effects of TAK981 in sepsis are still unclear, but it has been found to improve survival in mild polymicrobial peritonitis and enhance immune responses.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Kai Nan, Jun-peng Pei, Li-hong Fan, Yuan-kai Zhang, Xin Zhang, Kang Liu, Zhi-bin Shi, Xiao-qian Dang, Kun-zheng Wang
Summary: Resveratrol can reverse abnormal osteogenesis/osteoclastogenesis activity by upregulating miR-146a and stabilizing osteogenesis/osteoclastogenesis homeostasis through Wnt/FOXO and Sirt1/NF-κB pathways.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Wenliang Song, Tiening Zhang, Ni Yang, Tao Zhang, Ri Wen, Chunfeng Liu
Summary: Inhibition of miR-122-5p mitigates sepsis-triggered myocardial injury by reducing inflammation, oxidative stress and apoptosis. This study reveals that miR-122-5p regulates cardioprotection through targeting GIT1 and involving the Nrf-2/HO-1 signaling pathway.
Article
Biotechnology & Applied Microbiology
Jiaqiong Li, Hongyang Xu, Na Li, Wenjing Du, Junxiang Ti, Jingyu Chen
Summary: This study found that GAS5 is downregulated in sepsis-ALI and inhibits cell apoptosis by up-regulating the expression of miR-146a.
Article
Cell Biology
Mingzhuang Hou, Yijian Zhang, Xinfeng Zhou, Tao Liu, Huilin Yang, Xi Chen, Fan He, Xuesong Zhu
Summary: Kartogenin (KGN) has been shown to protect against cartilage degradation in an OA mouse model by promoting the synthesis of cartilage matrix components and suppressing the expression of matrix degradation enzymes. This protective effect is mediated by the miR-146a-NRF2 axis, highlighting KGN as a potential therapeutic agent for OA-induced cartilage degeneration.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Ke Ma, Jin-Feng Liu, Zi-Run Zheng, Hong-Yue Li, Bo Hu, Yu Meng
Summary: Macrophages play a significant role in acute kidney injury in sepsis patients, but their specific role is still unclear. This study found that polarization of M2 macrophages after methylprednisolone injection can significantly alleviate acute kidney injury.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Chemistry, Medicinal
Danhong Pan, Jinqiang Zhu, Liexiang Cao, Beilei Zhu, Lili Lin
Summary: Ruscogenin (RUS) exerts protective effects on sepsis-induced endothelial dysfunction (ED) by suppressing miR-146a-5p.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Huang Huang, Jing Zhu, Lili Gu, Jiang Hu, Xiujing Feng, Weiliang Huang, Sheng Wang, Yang Yang, Ping Cui, Shao-Hsuan Lin, Andrew Suen, Briana K. Shimada, Brittney Williams, Maureen A. Kane, Yunbo Ke, Chen-Ou Zhang, Anna A. Birukova, Konstantin G. Birukov, Wei Chao, Lin Zou
Summary: Research demonstrates that TLR7 activation can induce pulmonary inflammation and endothelial barrier disruption through sensing extracellular miR-146a-5p, thus contributing to sepsis-associated acute respiratory distress syndrome.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Junbo Yu, Jianhua Xue, Chun Liu, Aixian Zhang, Lili Qin, Jiajia Liu, Yang Yang
Summary: miR-146a-5p plays a regulatory role in sepsis by promoting disease progression through DCs activation and targeting ATG7.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2022)
Article
Pharmacology & Pharmacy
Shanping Wang, Keai Sinn Tan, Huimin Beng, Fei Liu, Jiandong Huang, Yihe Kuai, Rui Zhang, Wen Tan
Summary: STV-Na has positive effects on the treatment of sepsis, improving the survival rate and function of multiple organs in mice. Additionally, it can reduce the production of inflammatory cytokines and macrophage infiltration.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Medicine, Research & Experimental
Jinli Ding, Yan Zhang, Xiaopeng Cai, Yi Zhang, Sisi Yan, Jiayu Wang, Sainan Zhang, Tailang Yin, Chaogang Yang, Jing Yang
Summary: The study revealed that M1 macrophages suppressed trophoblast EMT and reduced their migration and invasion abilities by secreting extracellular vesicles (EVs). miR-146a-5p and miR-146b-5p were identified as the most upregulated miRNAs in trophoblasts treated with M1-EVs, playing a role in inhibiting trophoblast EMT by directly suppressing TNF receptor-associated factor 6 (TRAF6) expression. Aberrant expression of miR-146a-5p, miR-146b-5p, and TRAF6 was found in placental villous tissues from patients with RSA, with negative correlations between miR-146a-5p/miR-146b-5p and TRAF6 expression levels.
Article
Biochemistry & Molecular Biology
Chao Fang, Pan Ren, Ganlan Bian, Jian Wang, Jiaxin Bai, Jiaxing Huang, Yixiao Ding, Xueyong Li, Mingkai Li, Zheng Hou
Summary: Sepsis is a major cause of in-hospital mortality, and understanding the metabolic reprogramming of macrophages and its impact on immune response is crucial for sepsis research. This study identified Spns2 as a key metabolic mediator in regulating inflammation through the lactate-ROS axis. Spns2 deficiency enhances glycolysis and intracellular lactate production, leading to increased pro-inflammatory response and hyperinflammation in early sepsis, as well as immunosuppression in the late stage of infection.
Article
Pathology
Qiang Lu, Dinggao Zhang, Hui Liu, Hao Xu
Summary: In this study, miR-942-5p was found to protect against LPS-induced cell injury in A549 cells by inhibiting apoptosis and inflammation through negatively regulating TRIM37.
INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY
(2021)
Review
Medicine, Research & Experimental
Qian Wang, Gangyu Long, Hong Luo, Xiqun Zhu, Yang Han, You Shang, Dingyu Zhang, Rui Gong
Summary: Studies have shown that S100A8/A9 plays an important role in sepsis and may serve as a potential biomarker for diagnosis and treatment. It is a molecule mainly found in neutrophils and monocytes, with pro-inflammatory and immunosuppressive properties.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Urology & Nephrology
Jessica F. Hebert, Yoshio Funahashi, Michael P. Hutchens
Summary: The transition from acute kidney injury (AKI) to chronic kidney disease (CKD) is widely accepted, and similar data support the development of diseases in other organ systems after AKI. Awareness of these other-organ sequelae may improve the care of patients after AKI. Recent studies show that stroke, hypertension, reproductive risk, dementia, and death (SHReDD) are sequelae that occur with increased risk within 6 months to 3 years after AKI diagnosis. Mechanistic studies suggest that kidney-centric mechanisms are drivers of long-term, extra-renal changes. Further research is necessary for better understanding and potential translational impact.
CURRENT OPINION IN NEPHROLOGY AND HYPERTENSION
(2023)
Article
Medicine, General & Internal
Yuki Kuroyanagi, Asami Takeda, Hiroshi Kinashi, Yoshiyuki Kuroyanagi, Minako Murata, Kenta Futamura, Norihiko Goto, Yoshihiko Watarai, Takuji Ishimoto, Kunio Morozumi, Yasuhiko Ito
Summary: The deposition of Galactose-deficient IgA1 (Gd-IgA1) was found in all transplanted kidneys, with latent IgA deposition noted in otherwise healthy kidneys. IgA deposition disappeared 1 year after kidney transplantation in most cases, but re-deposition was observed in a few cases. The deposition of Gd-IgA1 may indicate the initial stage of IgA nephropathy, but additional factors such as IgG deposition are required for the ultimate development of IgA nephropathy.
Article
Medicine, Research & Experimental
Shunnosuke Kunoki, Hideki Tatsukawa, Yukinao Sakai, Hiroshi Kinashi, Tetsuyoshi Kariya, Yasuhiro Suzuki, Masashi Mizuno, Makoto Yamaguchi, Hiroyuki Sasakura, Masashi Ikeno, Kosei Takeuchi, Takuji Ishimoto, Kiyotaka Hitomi, Yasuhiko Ito
Summary: Long-term peritoneal dialysis is often associated with peritoneal dysfunction characterized by fibrosis, inflammation, and angiogenesis. In this study, transglutaminase 2 (TG2) was identified as a potential therapeutic target for peritoneal injury. TG2 activity and protein expression were found to increase during the development of peritoneal fibrosis, and TGF-β and TG2 interaction played a role in regulating peritoneal fibrosis, angiogenesis, and inflammation. Inhibition of TG2 showed promising results in reducing peritoneal injuries associated with PD.
LABORATORY INVESTIGATION
(2023)
Article
Medicine, General & Internal
Makoto Yamaguchi, Masashi Mizuno, Fumiya Kitamura, Shiho Iwagaitsu, Hironobu Nobata, Hiroshi Kinashi, Shogo Banno, Akimasa Asai, Takuji Ishimoto, Takayuki Katsuno, Yasuhiko Ito
Summary: Thrombotic microangiopathy (TMA) and macrophage activation syndrome (MAS) are rare and life-threatening complications of systemic lupus erythematosus (SLE). The association between TMA and MAS in SLE patients has been rarely studied and the diagnosis of these conditions remains challenging. This case report demonstrates the efficacy of eculizumab in treating refractory TMA concurrent with SLE-associated MAS, and suggests the importance of evaluating TMA during the course of SLE-associated MAS.
FRONTIERS IN MEDICINE
(2023)
Article
Urology & Nephrology
Miyoko Kunou, Makoto Yamaguchi, Hirotake Takahashi, Yukihiro Kimura, Naoki Watanabe, Mayumi Ito, Hirokazu Sugiyama, Shiho Iwagaitsu, Hironobu Nobata, Hiroshi Kinashi, Takayuki Katsuno, Shogo Banno, Yasuhiko Ito, Takuji Ishimoto
Summary: Adenine phosphoribosyltransferase (APRT) deficiency, a rare genetic disorder, causes the accumulation of poorly soluble 2,8-dihydroxyadenine (DHA) in the kidneys, leading to various renal problems such as nephrolithiasis, acute kidney injury, and chronic kidney disease (CKD) due to crystal nephropathy. We present a case of a 43-year-old man with 2,8-DHA crystalline nephropathy caused by APRT deficiency, diagnosed definitively by urine gas chromatography-mass spectrometry (GC/MS)-based plasma metabolomics. This case highlights the importance of recognizing the signs, symptoms, and progression of this rare condition to CKD, which can be prevented by early administration of xanthine oxidoreductase inhibitors.
Article
Pharmacology & Pharmacy
Tomoko Mizaki, Hironobu Nobata, Shogo Banno, Makoto Yamaguchi, Hiroshi Kinashi, Shiho Iwagaitsu, Takuji Ishimoto, Yukiko Kuru, Masafumi Ohnishi, Ken-ichi Sako, Yasuhiko Ito
Summary: This study developed the first PPK model of MPA for Japanese lupus nephritis patients, and found that a double sampling strategy at 1 and 4 hours, as well as a single sampling strategy at 6 hours, were the most effective methods. These limited sampling strategies may facilitate safer, more effective, and individualized therapy.
JOURNAL OF PHARMACEUTICAL HEALTH CARE AND SCIENCES
(2023)
Article
Urology & Nephrology
Sumihisa Ono, Naoyuki Hatayama, Kanyu Miyamoto, Munekazu Naito, Takuji Ishimoto, Yasuhiko Ito
Summary: This study used a simple rat model to investigate the growth of endothelial cells on the luminal surface of arteriovenous grafts. The results showed that the formation of endothelial cells was initially more active on the venous side and then increased in number across the luminal surface. Future clinical studies could confirm whether puncture on the arterial side versus the venous side results in different infection rates.
CLINICAL AND EXPERIMENTAL NEPHROLOGY
(2023)
Article
Urology & Nephrology
Takehiko Wada, Sayaka Shimizu, Masahiro Koizumi, Tadashi Sofue, Hiroki Nishiwaki, Sho Sasaki, Izaya Nakaya, Yuji Oe, Takuji Ishimoto, Kengo Furuichi, Hirokazu Okada, Noriaki Kurita
Summary: This study examined nephrologists' adherence to the recommendations of the Evidence-Based Clinical Practice Guideline for Nephrotic Syndrome 2020. A web-based survey was conducted among certified nephrologists in Japan. The findings revealed gaps between recommendations and practices, particularly in serodiagnosis and treatment of membranous nephropathy.
CLINICAL AND EXPERIMENTAL NEPHROLOGY
(2023)
Article
Medicine, General & Internal
Reiko Muto, Yasuhiro Suzuki, Hideaki Shimizu, Kaoru Yasuda, Takuji Ishimoto, Shoichi Maruyama, Yasuhiko Ito, Masashi Mizuno
Summary: Fabry disease is a lysosomal storage disorder caused by mutations in the alpha-galactosidase A gene. In Fabry disease patients with end-stage renal disease (ESRD), cerebrovascular events are often fatal even with enzyme replacement therapy (ERT). The use of biomarkers to assess the response to ERT in such cases is unclear. A case of recurrent cerebrovascular complications under ERT in a Fabry disease patient progressing to ESRD on peritoneal dialysis is reported, suggesting that careful long-term follow-up is needed for Fabry disease patients with ESRD receiving ERT and exhibiting cerebrovascular manifestations.
Review
Biochemistry & Molecular Biology
Yasuhiko Ito, Ting Sun, Hiroya Tanaka, Makoto Yamaguchi, Hiroshi Kinashi, Fumiko Sakata, Shunnosuke Kunoki, Yukinao Sakai, Takuji Ishimoto
Summary: High salt intake contributes to over-hydration in chronic kidney disease (CKD) patients, and sodium storage in tissues, regulated by the TonEBP protein, is associated with inflammation, fibrosis, and organ dysfunction. Salt-loading subtotal nephrectomized mice showed macrophage infiltration and upregulated TonEBP expression in the heart and peritoneal wall, leading to cardiac fibrosis and peritoneal membrane dysfunction. Reducing salt intake and using anti-interleukin-6 therapy attenuated these effects. Further studies are needed to understand and regulate TonEBP activation in CKD patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Kentaro Imai, Takuji Ishimoto, Tomohito Doke, Toshiki Tsuboi, Yu Watanabe, Keisuke Katsushima, Miho Suzuki, Hideto Oishi, Kazuhiro Furuhashi, Yasuhiko Ito, Yutaka Kondo, Shoichi Maruyama
Summary: This study investigated the function of lncRNAs in renal interstitial fibrosis (RIF), a common manifestation of chronic kidney diseases. The lncRNA lnc-CHAF1B-3 was found to be significantly upregulated in human renal proximal tubular cells when stimulated with TGF-β1 and hypoxia, accompanied by increased expression of EMT-related genes. Knockdown of lnc-CHAF1B-3 suppressed the upregulated expression of various genes related to RIF progression. This suggests that lnc-CHAF1B-3 may be a potential target for treating RIF.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Nutrition & Dietetics
Makoto Yamaguchi, Mayumi Ito, Hirokazu Sugiyama, Shiho Iwagaitsu, Hironobu Nobata, Hiroshi Kinashi, Takayuki Katsuno, Shogo Banno, Yasuhiko Ito, Masahiko Ando, Yoko Kubo, Keisuke Maeda, Yuria Ishida, Naoharu Mori, Takuji Ishimoto
Summary: A study found that malnutrition based on the GLIM criteria is associated with mortality in patients with cardiovascular disease (CVD), and the severity of malnutrition is positively correlated with mortality. Furthermore, malnutrition combined with renal dysfunction is also positively associated with mortality. These findings provide clinically relevant information for identifying high mortality risk and emphasizing the importance of addressing malnutrition with kidney dysfunction among CVD patients.
CLINICAL NUTRITION ESPEN
(2023)
Review
Rheumatology
Seika Terashima, Makoto Yamaguchi, Tomomi Ishihara, Junichiro Hagita, Takayuki Katsuno, Mayumi Ito, Hirokazu Sugiyama, Shiho Iwagaitsu, Hironobu Nobata, Hiroshi Kinashi, Takuji Ishimoto, Shogo Banno, Yasuhiko Ito
Summary: Eosinophilic granulomatosis with polyangiitis (EGPA) is a rare disease characterized by inflammation in multiple organ systems, most commonly the lungs, skin, and nervous system. Limb ulcers are a rare complication, and there are few reports on the use of mepolizumab in treating EGPA.
MODERN RHEUMATOLOGY CASE REPORTS
(2023)
Article
Physiology
Hiroya Tanaka, Ting Sun, Hiroshi Kinashi, Keisuke Kamiya, Makoto Yamaguchi, Hironobu Nobata, Fumiko Sakata, Hangsoo Kim, Masashi Mizuno, Shunnosuke Kunoki, Yukinao Sakai, Akiyoshi Hirayama, Tomoyoshi Soga, Kazuhiro Yoshikawa, Takuji Ishimoto, Yasuhiko Ito
Summary: In this study, IL-6 blockade was found to have anti-inflammatory, antifibrotic, and partial antioxidative effects on the hearts of mice with CKD on a high-salt diet. Therefore, IL-6 potentially mediates cardiac fibrosis induced by high salt intake in patients with CKD, a finding with therapeutic implications.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2022)
Article
Rheumatology
Fumiya Kitamura, Makoto Yamaguchi, Minori Nishimura, Takayuki Katsuno, Mayumi Ito, Hirokazu Sugiyama, Shiho Iwagaitsu, Hironobu Nobata, Hiroshi Kinashi, Takuji Ishimoto, Shogo Banno, Yasuhiko Ito
Summary: This case report presents a patient with anti-neutrophil cytoplasmic antibody-associated vasculitis-related thrombotic microangiopathy who showed significant improvement after treatment with eculizumab. It suggests that complement activation may be involved in the pathogenesis of this disease, and prompt use of eculizumab may be an effective strategy for preventing organ damage.
MODERN RHEUMATOLOGY CASE REPORTS
(2022)
Article
Medicine, Research & Experimental
Marijn A. Scheijde-Vermeulen, Lennart A. Kester, Liset Westera, Bastiaan B. J. Tops, Friederike A. G. Meyer-Wentrup
Summary: This study aimed to evaluate the feasibility of integrating state-of-the-art sequencing techniques and flow cytometry into the diagnostic workup of pediatric lymphoma. The results showed that this integration is not only feasible but also provides additional diagnostic information.
LABORATORY INVESTIGATION
(2024)
Article
Medicine, Research & Experimental
Enrico Berrino, Sara Erika Bellomo, Anita Chesta, Paolo Detillo, Alberto Bragoni, Amedeo Gagliardi, Alessio Naccarati, Matteo Cereda, Gianluca Witel, Anna Sapino, Benedetta Bussolati, Gianni Bussolati, Caterina Marchi
Summary: Formalin-fixed paraffin-embedded (FFPE) samples are crucial for tissue-based analysis in precision medicine, but the quality of these samples can affect the reliability of sequencing data. The use of acid-deprived fixatives guarantees the highest DNA preservation and sequencing performance, enabling more complex molecular profiling of tissue samples.
LABORATORY INVESTIGATION
(2024)
Article
Medicine, Research & Experimental
Roope A. Kallionpaa, Sirkku Peltonen, Kim My Le, Eija Martikkala, Mira Jaaskelainen, Elnaz Fazeli, Pilvi Riihila, Pekka Haapaniemi, Anne Rokka, Marko Salmi, Ilmo Leivo, Juha Peltonen
Summary: This study investigated the immune microenvironment of cutaneous neurofibromas (cNFs) in patients with neurofibromatosis 1 (NF1). The results showed that cNFs have substantial populations of T cells and macrophages, which may be tumor-specific. T cell populations in cNFs were found to be different from those in the skin, and cNFs exhibited lower expression of proteins related to T cell-mediated immunity compared to the skin.
LABORATORY INVESTIGATION
(2024)