Article
Infectious Diseases
Tijs K. Tournoy, Anna Rosanas-Urgell, Marjan Van Esbroeck, Emmanuel Bottieau, Ralph Huits
Summary: We reported a case of Plasmodium malariae malaria in a traveler who returned from the Democratic Republic of Congo to Belgium. This case occurred despite the individual receiving successful treatment for Plasmodium falciparum three weeks earlier and not being re-exposed to the infection in an endemic area. We discussed possible explanations for this unusual observation.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Tijs K. Tournoy, Anna Rosanas-Urgell, Marjan Van Esbroeck, Emmanuel Bottieau, Ralph Huits
Summary: A traveler was diagnosed with Plasmodium malariae malaria despite having received successful treatment for Plasmodium falciparum three weeks earlier and without re-exposure in an endemic area. Possible explanations for this uncommon observation were discussed.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Review
Chemistry, Analytical
Vasileios Alampanos, Victoria Samanidou
Summary: Sample preparation and extraction play a crucial role in bioanalytical methods, particularly for the analysis of parabens in human biological samples. Parabens, commonly used as preservatives, are known for their antimicrobial activity but also their potential endocrine disrupting effects, making their determination in complex biological matrices a challenging task. Efforts in developing efficient extraction protocols coupled with liquid chromatography are essential for accurate analysis and further epidemiological and toxicological studies.
MICROCHEMICAL JOURNAL
(2021)
Article
Pharmacology & Pharmacy
M. Silva, M. Malmberg, S. D. Otienoburu, A. Bjorkman, B. Ngasala, A. Martensson, J. P. Gil, M. I. Veiga
Summary: The study found significant co-expression patterns of the pfmdr1 and pfcrt genes before and during ACT treatment. This co-expression may play an important role when the parasite is exposed to drug stress.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Fabiana Volpe-Zanutto, Leticia Tiburcio Ferreira, Andi Dian Permana, Melissa Kirkby, Alejandro J. Paredes, Lalitkumar K. Vora, Amanda P. Bonfanti, Ives Charlie-Silva, Catarina Raposo, Mariana C. Figueiredo, Ilza M. O. Sousa, Andi Brisibe, Fabio Trindade Maranhao Costa, Ryan F. Donnelly, Mary Ann Foglio
Summary: Malaria affects over 200 million people annually and artemether and lumefantrine are the primary treatment options. Dissolving microneedles were developed to deliver the drugs intradermally, with studies in animals demonstrating significant efficacy.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Public, Environmental & Occupational Health
Vy Bui, Colleen R. Higgins, Sarah Laing, Sachiko Ozawa
Summary: This study examines the impact of substandard and falsified antimalarials on health and economy in Benin, showing the significant cost and death associated with poor-quality antimalarials. Replacing all antimalarials with quality-assured artemisinin combination therapies has the potential to save costs and prevent deaths.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2022)
Article
Engineering, Biomedical
Fabiana Volpe-Zanutto, Bruno Fonseca-Santos, Peter E. McKenna, Alejandro J. Paredes, Jose Luis Davila, Maeliosa T. C. McCrudden, Marcelo Marucci Pereira Tangerina, Mariana Ceccheto Figueiredo, Wagner Vilegas, Andi Brisibe, Marcos Akira D'Avila, Ryan F. Donnelly, Marlus Chorilli, Mary Ann Foglio
Summary: Artemether (ART) and lumefantrine (LUM) are commonly used antimalarial drugs, typically administered orally. However, a new surfactant-based ART-LUM formulation (S3AL) has shown potential for transdermal delivery, improving drug bioavailability and skin adhesion. The addition of LUM to the formulation changed its structure and showed biocompatibility with human keratinocyte cells.
BIOMEDICAL MATERIALS
(2021)
Article
Public, Environmental & Occupational Health
Gabriel Kishoyian, Eliud N. M. Njagi, George O. Orinda, Francis T. Kimani, Kevin Thiongo, Damaris Matoke-Muhia
Summary: After a decade of use, artemether-lumefantrine remains highly effective in clearing Plasmodium falciparum parasites in children aged between 6 and 60 months in Kenya. Continued monitoring is essential to prevent potential resistance to the drug in all target users.
EPIDEMIOLOGY AND INFECTION
(2021)
Article
Medicine, General & Internal
Gaston Tona Lutete, Ghyslain Mombo-Ngoma, Serge-Brice Assi, Jude D. Bigoga, Felix Koukouikila-Koussounda, Nsengi Y. Ntamabyaliro, Francine Ntoumi, Selidji T. Agnandji, Mirjam Groger, Jangsik Shin, Isabelle Borghini-Fuhrer, Sarah Arbe-Barnes, Stephen J. Allen, Peter G. Kremsner, Robert Miller, Stephan Duparc, Michael Ramharter
Summary: In a study conducted in Africa, pyronaridine-artesunate showed good tolerability and effectiveness in the treatment of acute uncomplicated malaria under conditions similar to everyday clinical practice, supporting its use as a useful addition in managing this condition.
Article
Medicine, General & Internal
Catherine Segbefia, Seth Kwabena Amponsah, Adwoa K. A. Afrane, Mame Yaa Nyarko, Yvonne Brew, Nihad Salifu, Samuel Yao Ahorhorlu, Abdul Malik Sulley, Lars Hviid, Michael Fokuo Ofori, George Obeng Adjei
Summary: In this study, interactions between hydroxyurea (HU) and antimalarials in sickle cell disease (SCD) were explored. The study found that parasite clearance and adverse event occurrence were similar in SCD children treated with artemether-lumefantrine (AL) regardless of HU status. However, distinct patterns of changes in laboratory indices suggest the potential need for larger, more focused studies in the future.
FRONTIERS IN MEDICINE
(2023)
Article
Infectious Diseases
Guoming Li, Yueming Yuan, Shaoqin Zheng, Chenguang Lu, Mingqiang Li, Ruixiang Tan, Hongying Zhang, Rahamatou Silai, Ruimei Liu, Kamal Said Abdallah, Affane Bacar, Qin Xu, Jianping Song, Wanting Wu, Changsheng Deng
Summary: The study found that artemisinin-piperaquine and artemether-lumefantrine maintained high efficacy and tolerance in treating uncomplicated falciparum malaria in the Comoros. However, asymptomatic malaria infections may pose new challenges to malaria control.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Infectious Diseases
Dominic Mosha, Mwaka A. Kakolwa, Muhidin K. Mahende, Honorati Masanja, Salim Abdulla, Chris Drakeley, Roland Gosling, Joyce Wamoyi
Summary: The study aimed to explore the acceptance, reliability, and perceived effectiveness of the single-low dose primaquine and the monitoring pharmacovigilance tool PROMPT. The results showed general acceptance and perceived effectiveness of primaquine, with safety concerns and challenges in administering the drug to children. Further validation involving the National Malaria Control Programme is essential for facilitating primaquine adoption in national policies.
Article
Public, Environmental & Occupational Health
Tamar Grossman, Julia Vainer, Yael Paran, Liora Studentsky, Uri Manor, Ron Dzikowski, Eli Schwartz
Summary: A study on artemisinin resistance genes in Southeast Asia and Africa found that the P76S mutation in the Pfcoronin gene is associated with treatment failure of the antimalarial drug AL, while mutations in the Pfkelch13 gene are not associated with treatment failure.
JOURNAL OF TRAVEL MEDICINE
(2023)
Article
Infectious Diseases
Luana C. Mathieu, Prabhjot Singh, Wuelton Marcelo Monteiro, Magda Magris, Horace Cox, Yassamine Lazrek, Gisely C. Melo, Paola Marchesini, Jean S. F. Alexandre, Angel Manuel Alvarez, Magalie Demar, Maylis Douine, Maria-Paz Ade, Marcus V. G. Lacerda, Lise Musset
Summary: The study found that there are few pfk13 mutant foci in Amazon basin countries, but there is a high risk of mutation dispersion mainly from the Guiana Shield due to mining activities.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Review
Pharmacology & Pharmacy
Phoebe Hazenberg, Kate Navaratnam, Paula Busuulwa, Catriona Waitt
Summary: Significant anatomical and physiological changes occur during pregnancy and labor, impacting drug absorption, distribution, metabolism, and elimination. Limited data are available on pregnancy-specific pharmacokinetics of anti-infectives, with further research needed to understand the clinical implications of pharmacokinetic differences between pregnant and nonpregnant individuals. Challenges in applying quality grading systems to these studies highlight the need for a refined appraisal system for pharmacokinetic studies in special populations.
CLINICAL PHARMACOLOGY & THERAPEUTICS
(2021)
Article
Thermodynamics
Maria Betania de Freitas-Marques, Barbara Caroline Rodrigues Araujo, Pedro Henrique Reis da Silva, Christian Fernandes, Wagner da Nova Mussel, Rita de Cassia de Oliveira Sebastiao, Maria Irene Yoshida
Summary: Molecularly imprinted polymers (MIPs) are used to enhance selectivity in solid-phase extraction, with synthetic MIPs composed of 2-vinyl pyridine, ethylene glycol dimethacrylate, toluene, and azobisisobutyronitrile. The study investigates the non-isothermal interactions between MIP and the antimalarial drug lumefantrine, identifying a specific thermal event for the compound. Activation energies for compound formation and conversion process were determined, with significant contributions from the Avrami-Erofeev kinetic model.
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2021)
Review
Biochemical Research Methods
Pedro Henrique Reis da Silva, Armando da Silva Cunha Junior, Gerson Antonio Pianetti, Christian Fernandes
Summary: Glaucoma is a heterogeneous group of optic neuropathies leading to irreversible blindness, mainly treated with pharmacotherapy to lower intraocular pressure. Studying ocular pharmacokinetics of antiglaucoma drugs is crucial for drug development, with bioanalysis often conducted in rabbits using aqueous humor as the matrix. Challenges in ocular bioanalysis include sample preparation methods and the need for more eco-friendly and non-terminal approaches. Ultra high-performance liquid chromatography and mass spectrometry are recommended for separation and detection, providing high selectivity and sensitivity in an environmentally friendly manner.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Graziella Gomes Rivelli, Andre Coura Perez, Pedro Henrique Reis Silva, Elionai Cassiana de Lima Gomes, Carolina Paula de Souza Moreira, Edwin Tamashiro, Fabiana Cardoso Pereira Valera, Wilma Terezinha Anselmo-Lima, Gerson Antonio Pianetti, Armando Silva-Cunha
Summary: The study developed biodegradable polymeric nanofibers and optimized their preparation conditions using Design of Experiments and Response Surface Methodology. In vivo tests demonstrated that polymeric nanofibers containing mometasone furoate are safe for treating chronic rhinosinusitis in the sinonasal mucosa.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Chemistry, Applied
Leila Rodrigues Caldeira, Fernando Diniz Madureira, Thalita De Faria Maia, Carlos Vitor Muller, Christian Fernandes
Summary: An analytical method for the simultaneous determination of ethylene glycol and diethylene glycol in beer was developed and validated, meeting the requirements of current legislation with high precision and accuracy. The method was successfully applied to a large number of beer samples.
Article
Pharmacology & Pharmacy
Guilherme S. Ramos, Virginia M. R. Vallejos, Gabriel S. M. Borges, Raquel M. Almeida, Izabela M. Alves, Marta M. G. Aguiar, Christian Fernandes, Pedro P. G. Guimaraes, Ricardo T. Fujiwara, Philippe M. Loiseau, Lucas A. M. Ferreira, Frederic Frezard
Summary: This study developed a formulation of PEGylated liposomal AmB and compared its efficacy to AmBisome (R) in a murine model of cutaneous leishmaniasis (CL). The results showed that the PEGylated formulation significantly reduced lesion growth and parasite load in the treatment of CL, both through parenteral and oral routes.
Article
Chemistry, Analytical
Mateus Araujo Castro e Souza, Naialy Fernandes Araujo Reis, Isabella Cristina Placido de Oliveira Pacheco, Maria Auxiliadora Parreiras Martins, Maria Beatriz A. Gloria, Gerson Antonio Pianetti, Christian Fernandes
Summary: This study developed a modern method for the simultaneous determination of eight cardiovascular drugs in human plasma. The method is simple, fast, environmentally friendly, and has high accuracy and reliability.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Analytical
Jessica Mauricio Batista, Melina Luiza Vieira Diniz, Wellington Marcos da Silva, Maria Beatriz A. Gloria, Edesia Martins Barros de Sousa, Christian Fernandes
Summary: In this study, boron nitride nanotubes functionalized with octadecyl groups were used as sorbent for extraction of losartan and valsartan from human plasma for the first time. The method was validated and showed good selectivity, precision, accuracy, and linearity. It is suitable for the quantification of losartan in plasma samples from patients under antihypertensive therapy, and can be used in therapeutic drug monitoring, pharmacokinetics, and bioequivalence studies.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Biotechnology & Applied Microbiology
Izabela Mansur-Alves, Brenda Lorrayne Furtado Lima, Thais Tunes Santos, Naialy F. Araujo, Frederic Frezard, Arshad Islam, Andre L. B. de Barros, Delia C. M. dos Santos, Christian Fernandes, Lucas A. M. Ferreira, Marta M. G. Aguiar
Summary: This study aimed to evaluate the influence of cholesterol on the aggregation state of NE-AmB. The results showed that cholesterol reduced the aggregation state of AmB. NE-AmB was stable and had lower hemolytic toxicity, and it was capable of stabilizing the lesion size and reducing the parasitic load in animals.
Article
Pharmacology & Pharmacy
Matheus Augusto de Castro, Pedro Henrique Reis, Christian Fernandes, Ricardo Geraldo de Sousa, Thomas Toshio Inoue, Silvia Ligorio Fiaiho, Armando Silva-Cunha
Summary: Ocular chemical burns can be effectively treated with a poloxamer-based thermoresponsive in-situ gelling system containing hyaluronic acid and indomethacin. The optimized system (F2) has a gelling temperature of 34.2 degrees C and shows improved wound healing in the ocular surface.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Gabriel Silva Marques Borges, Pierre Sicard, Cristina de Mello Gomides Loures, Fernanda Gontijo Cristina Evangelista, Camila Campos Sales, Adriano de Paula Sabino, Christian Fernandes, Lucas Antonio Miranda Ferreira, Sylvain Richard
Summary: All-trans retinoic acid and arsenic trioxide are effective treatments for acute promyelocytic leukemia, but they have certain drawbacks such as patient relapse and cardiotoxicity. In this study, a self-nanoemulsifying drug delivery system enriched with tocotrienols was developed to enhance the cytotoxicity of all-trans retinoic acid and mitigate the cardiotoxic effects of arsenic trioxide. The system showed promising results in improving the antileukemic properties of all-trans retinoic acid, but it also influenced the electrocardiogram changes caused by arsenic trioxide administration.
Article
Chemistry, Medicinal
Maria Betania de Freitas-Marques, Talita Santos do Valle, Barbara Caroline Rodrigues de Araujo, Rita de Cassia de Oliveira Sebastiao, Wagner da Nova Mussel, Maria Irene Yoshida, Christian Fernandes
Summary: This study evaluated the thermal energy and tableting effects of benznidazole (BZN), excipients, and tablets using techniques such as infrared spectroscopy, X-ray diffraction, and thermal analysis. The results revealed the dehydration of talc and alpha-lactose monohydrate and the conversion of beta-lactose to stable alpha-lactose during tableting. The findings highlight the importance of considering thermal energy and tableting effects in the manufacturing of BZN.
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
(2023)
Article
Biochemical Research Methods
Melina Luiza Vieira Diniz, Jessica Mauricio Batista, Pedro Henrique Reis da Silva, Christian Fernandes
Summary: This study aimed to develop and validate a MEPS coupled to high performance liquid chromatography method for the analysis of benznidazole in human plasma. The method was proved to be selective, precise, accurate, robust and linear in the range from 0.5 to 6.0 μg mL-1, and it was applied successfully to assess this drug in plasma samples.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Ophthalmology
Rodrigo Jorge, Isabela Franco Villela, Christian Fernandes, Thais Marino de Azevedo Bastos, Ingrid U. Scott, Armando da Silva Cunha, Silvia Ligorio Fialho, Pedro Henrique Reis da Silva, Andre Messias
Summary: This article reports a case of pseudophakic cystoid macular edema (PCME) in which conventional treatment was ineffective but improved after intravitreal acetazolamide implant injection. Complete resolution of symptoms was observed 4 weeks after the injection. Further investigation is needed to confirm these findings.
DOCUMENTA OPHTHALMOLOGICA
(2023)
Article
Chemistry, Multidisciplinary
Luan F. Diniz, Paulo S. Carvalho, Jose E. Goncalves, Renata Diniz, Christian Fernandes
Summary: This study focuses on expanding the solid-state landscape of metformin hydrochloride (MET-HCl) and designing three novel MET salts. The biopharmaceutical profile of these salts, including MET-HCl, has been evaluated. The results show the potential of these solid forms as enhanced API candidates for new antidiabetic solid formulations.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)