Identification of steroid C27 monooxygenase isoenzymes involved in sterol catabolism and stepwise pathway engineering of Mycobacterium neoaurum for improved androst-1,4-diene-3,17-dione production

Efficient testosterone production by engineered Pichia pastoris co-expressing human 17β-hydroxysteroid dehydrogenase type 3 and Saccharomyces cerevisiae glucose 6-phosphate dehydrogenase with NADPH regeneration

(2016) Minglong Shao et al.   GREEN CHEMISTRY

Enhanced intracellular soluble production of 3-ketosteroid-Δ1-dehydrogenase fromMycobacterium neoauruminEscherichia coliand its application in the androst-1,4-diene-3,17-dione production

(2016) Minglong Shao et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

A mutant form of 3-ketosteroid-Δ1-dehydrogenase gives altered androst-1,4-diene-3, 17-dione/androst-4-ene-3,17-dione molar ratios in steroid biotransformations by Mycobacterium neoaurum ST-095

(2016) Minglong Shao et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Enhanced Production of Androst-1,4-Diene-3,17-Dione by Mycobacterium neoaurum JC-12 Using Three-Stage Fermentation Strategy

(2015) Minglong Shao et al.   PLoS One

Bioconversion of cholesterol to 4-cholesten-3-one by recombinantBacillus subtilisexpressingchoMgene encoding cholesterol oxidase fromMycobacterium neoaurumJC-12

(2014) Minglong Shao et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Characterization and engineering of 3-ketosteroid-△1-dehydrogenase and 3-ketosteroid-9α-hydroxylase in Mycobacterium neoaurum ATCC 25795 to produce 9α-hydroxy-4-androstene-3,17-dione through the catabolism of sterols

(2014) Kang Yao et al.   METABOLIC ENGINEERING

The rebalanced pathway significantly enhances acetoin production by disruption of acetoin reductase gene and moderate-expression of a new water-forming NADH oxidase in Bacillus subtilis

(2014) Xian Zhang et al.   METABOLIC ENGINEERING

Efficient Whole-Cell Biocatalyst for Acetoin Production with NAD+ Regeneration System through Homologous Co-Expression of 2,3-Butanediol Dehydrogenase and NADH Oxidase in Engineered Bacillus subtilis

(2014) Teng Bao et al.   PLoS One

Bioconversion of 4-androstene-3,17-dione to androst-1,4-diene-3,17-dione by recombinant Bacillus subtilis expressing ksdd gene encoding 3-ketosteroid-Δ1-dehydrogenase from Mycobacterium neoaurum JC-12

(2013) Wenqing Zhang et al.   JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY

Biotransformation of cholesterol to 1,4-androstadiene-3,17-dione (ADD) by Nocardia species

(2012) Preeti Sharma et al.   ANNALS OF MICROBIOLOGY

Microbial steroid transformations: current state and prospects

(2012) Marina V. Donova et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Characterization and application of fusidane antibiotic biosynethsis enzyme 3-ketosteroid-∆1-dehydrogenase in steroid transformation

(2012) Miao-Miao Chen et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Identification and engineering of cholesterol oxidases involved in the initial step of sterols catabolism in Mycobacterium neoaurum

(2012) Kang Yao et al.   METABOLIC ENGINEERING

Effects of hydroxypropyl-β-cyclodextrin on cell growth, activity, and integrity of steroid-transforming Arthrobacter simplex and Mycobacterium sp.

(2011) Yanbing Shen et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Multiplicity of 3-Ketosteroid-9 -Hydroxylase Enzymes in Rhodococcus rhodochrous DSM43269 for Specific Degradation of Different Classes of Steroids

(2011) M. Petrusma et al.   JOURNAL OF BACTERIOLOGY

Activity of 3-Ketosteroid 9α-Hydroxylase (KshAB) Indicates Cholesterol Side Chain and Ring Degradation Occur Simultaneously inMycobacterium tuberculosis

(2011) Jenna K. Capyk et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Cholesterol catabolism as a therapeutic target in Mycobacterium tuberculosis

(2011) Hugues Ouellet et al.   TRENDS IN MICROBIOLOGY

Inactivation and Augmentation of the Primary 3-Ketosteroid- 1- Dehydrogenase in Mycobacterium neoaurum NwIB-01: Biotransformation of Soybean Phytosterols to 4-Androstene- 3,17-Dione or 1,4-Androstadiene-3,17-Dione

(2010) W. Wei et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

A New Steroid-Transforming Strain of Mycobacterium neoaurum and Cloning of 3-Ketosteroid 9α-Hydroxylase in NwIB-01

(2010) Wei Wei et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Cholesterol biotransformation to androsta-1,4-diene-3,17-dione by growing cells of Chryseobacterium gleum

(2010) Priti N. Chaudhari et al.   BIOTECHNOLOGY LETTERS

Bioconversion of soysterols to androstenedione by Mycobacterium fortuitum subsp. fortuitum NCIM 5239, a mutant derived from total sterol degrader strain

(2010) Vrushali Gulla et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Mycobacterium tuberculosis CYP125A1, a steroid C27 monooxygenase that detoxifies intracellularly generated cholest-4-en-3-one

(2010) Hugues Ouellet et al.   MOLECULAR MICROBIOLOGY

Efficient biotransformation of cholesterol to androsta-1,4-diene-3,17-dione by a newly isolated actinomycete Gordonia neofelifaecis

(2010) Yuchang Liu et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Improvement of Steroid Biotransformation with Hydroxypropyl-β-Cyclodextrin Induced Complexation

(2009) Liting Zhang et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Mycobacterial Cytochrome P450 125 (Cyp125) Catalyzes the Terminal Hydroxylation of C27 Steroids

(2009) Jenna K. Capyk et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Androstenedione production by biotransformation of phytosterols

(2008) Alok Malaviya et al.   BIORESOURCE TECHNOLOGY