Review
Pharmacology & Pharmacy
Abigail Herrera-Ruiz, Benjamin Betancourt Tovar, Ruben Gutierrez Garcia, Maria Fernanda Leal Tamez, Narsimha Mamidi
Summary: GelMA-based composites have wide-ranging applications in biomedical field due to their exceptional properties, but their potential in bone tissue engineering is still limited. Further research and development are needed to design more efficient composites for bone regeneration, although challenges remain.
Article
Biochemistry & Molecular Biology
Ece Guler, Yaren Ezgi Baripoglu, Hussain Alenezi, Ayca Arikan, Ravana Babazade, Semra Unal, Gokhan Duruksu, Fawzan S. Alfares, Yusufhan Yazir, Faik Nuzhet Oktar, Oguzhan Gunduz, Mohan Edirisinghe, Muhammet Emin Cam
Summary: DKMF, loaded with Vitamin D3, Vitamin K2, and Mg, demonstrated osteoinductive effects by activating the Wnt/beta-catenin signaling pathway and promoting the growth and function of osteoblast cells, making it a promising treatment approach in bone tissue engineering.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Medicine, Research & Experimental
Bin Lv, Li Lu, Liangcong Hu, Peng Cheng, Yiqiang Hu, Xudong Xie, Guandong Dai, Bobin Mi, Xin Liu, Guohui Liu
Summary: Increasing data reveals the involvement of methacrylated gelatin in various physiologic processes for therapeutic interventions. Gelatin methacryloyl (GelMA) hydrogel, with its good biocompatibility, low cost, and photo-cross-linking structure, is an attractive bioink for cell survivability and monitoring. GelMA has extensive biomedical applications and recent advances focus on integrating bioactive nanomaterials to improve its properties. GelMA shows promise for musculoskeletal disorders and has been investigated for applications in osteoarthritis, intervertebral disc degeneration, bone regeneration, and tendon disorders.
Article
Biochemistry & Molecular Biology
Zhongxing Cai, Ziyi Guo, Chaohui Yang, Fei Wang, Peibiao Zhang, Yu Wang, Min Guo, Zongliang Wang, Jing Huang, Long Zhang
Summary: By hydroxylation, silylation, and glutamylation processes, we designed and synthesized polymer PBLG-modified GdPO4·H2O nanobunches to achieve smart biomedical micro/nanomaterials that interact with biomolecules, improve osteogenic/chondrogenic differentiation, exhibit better dispersion in bone implants, and maximize functionality.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Nanoscience & Nanotechnology
Lijia Cheng, Shoma K. Suresh, Hongyan He, Ritu Singh Rajput, Qiyang Feng, Saravanan Ramesh, Yuzhuang Wang, Sasirekha Krishnan, Serge Ostrovidov, Gulden Camci-Unal, Murugan Ramalingam
Summary: Recent advancements in 3D printing technology show great potential in fabricating scaffolds and implants for biomedical applications, particularly in bone repair and regeneration. The integration of different living cells in 3D constructs made from conventional biomaterials can create artificial bone grafts capable of regenerating damaged tissues. Various conventional and nanomaterials have been utilized in the production of 3D-printed scaffolds for bone tissue engineering applications.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Cell Biology
Katarzyna Klimek, Krzysztof Palka, Wieslaw Truszkiewicz, Timothy E. L. Douglas, Aleksandra Nurzynska, Grazyna Ginalska
Summary: This study aimed to evaluate the potential application of curdlan/whey protein isolate/hydroxyapatite biomaterials in bone tissue engineering and investigate the influence of whey protein isolate concentration on their properties. The results showed that the biomaterials exhibited some desirable characteristics for bone scaffolds, but their porosity and mechanical properties need to be improved.
Review
Pharmacology & Pharmacy
Xirui Jing, Zekang Xiong, Zian Lin, Tingfang Sun
Summary: Recent research and application of nanomaterials such as graphene, carbon nanotubes, and metal-organic frameworks in tissue engineering have gained popularity. Black phosphorus (BP), a two-dimensional sheet of BP crystals, was isolated in 2014 and has since emerged as a promising nanomaterial. BP possesses advantages such as light responsiveness, electrical conductivity, degradability, and good biocompatibility, making it a potential candidate for biomedical applications. Additionally, BP is composed of phosphorus, a key component in bone tissue with good biocompatibility and osteogenic repair ability. This review describes the structure and properties of BP, as well as its current applications in bone tissue engineering, to facilitate future research and utilization of BP.
Article
Engineering, Biomedical
Gun-Woo Oh, Van-Tinh Nguyen, Seong-Yeong Heo, Seok-Chun Ko, Chang Su Kim, Won Sun Park, Il-Whan Choi, Won-Kyo Jung
Summary: This study developed a novel method using PCL 3D scaffolds combined with fish collagen and abalone intestine gastro-intestinal digests, showing higher alkaline phosphatase activity and mineralization in PCL/AIGIDs/Col scaffolds. In vivo experiments demonstrated strong osteoinduction capability of the PCL/AIGIDs/Col scaffold in rabbit tibia defect models, suggesting promising potential for tissue implants and bone regeneration.
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
(2021)
Review
Biotechnology & Applied Microbiology
Duraipandy Natarajan, Zhitong Ye, Liping Wang, Linhu Ge, Janak Lal Pathak
Summary: The challenges in bone graft and prosthetic implant designing for clinical application are being addressed with the revolutionary advances of nanotechnology, particularly in the use of rare earth (RE) materials with unique properties for tissue engineering. Researchers have developed smart nano-biomaterials using RE materials for bone tissue engineering and implantology applications, exploring their molecular mechanisms and potential for novel strategies. The biomedical applications of micro or nano-scale RE materials have shown promise in providing cost-effective solutions in bone tissue engineering.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Article
Agricultural Engineering
Chotiwit Sriwong, Suwimon Boonrungsiman, Prakit Sukyai
Summary: Biocomposite cellulose-based scaffolds combined with hydroxyapatite were successfully developed from sugarcane bagasse for tissue engineering. The scaffolds showed appropriate porosity and low degradation rate. Cell viability, proliferation, and differentiation experiments demonstrated that the cellulose combined HA scaffolds promoted the growth and differentiation of bone cells. The results indicated that sugarcane bagasse-derived cellulose is an ideal material for bone scaffolding applications.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Biochemistry & Molecular Biology
Enrique Garcia-Recio, Victor J. Costela-Ruiz, Lucia Melguizo-Rodriguez, Javier Ramos-Torrecillas, Olga Garcia-Martinez, Concepcion Ruiz, Elvira de Luna-Bertos
Summary: This study investigates the impact of BPA on functional parameters of human osteoblasts and finds that BPA inhibits cell growth, alkaline phosphatase activity, and mineralization. In addition, BPA reduces the expression of osteogenic genes and increases the expression of CD54 and CD80 antigens. These findings suggest that BPA adversely affects osteoblast differentiation/maturation, proliferation, and functional capacity, potentially posing a risk to bone health.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Settimio Pacelli, Aparna R. Chakravarti, Saman Modaresi, Siddharth Subham, Kyley Burkey, Cecilia Kurlbaum, Madeline Fang, Christopher A. Neal, Adam J. Mellott, Aishik Chakraborty, Arghya Paul
Summary: The study utilized polydopamine (pDA) treatment as a binding platform to create a versatile gelatin-alginate (Gel-Alg) hydrogel, effectively controlling stem cell adhesion and osteogenic differentiation, demonstrating the potential application value in tissue engineering.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Engineering, Biomedical
Yamei Xu, Yao Yang, Ziyi Hua, Shuang Li, Zhenyu Yang, Qianzi Liu, Gang Fu, Ping Ji, Qingqing Wu
Summary: The BMP2 antibody shows promise as a replacement for rhBMP2 in bone tissue engineering, promoting bone formation and suppressing osteoclastogenesis. Further research is needed to clarify the specific osteogenic mechanism and adverse reactions associated with the antibody.
Article
Materials Science, Multidisciplinary
Hussein M. El-Husseiny, Eman A. Mady, Walaa A. El-Dakroury, Moataz B. Zewail, Mina Noshy, Amr M. Abdelfatah, Ahmed S. Doghish
Summary: Bone tissue engineering plays a crucial role in repairing bone defects by combining biomolecules, biomimetic scaffolds, and cells to provide smart and complex platforms that enhance the limited self-healing capacity.
APPLIED MATERIALS TODAY
(2022)
Review
Cell Biology
Xiaoying Chen, Wei Xie, Ming Zhang, Yuhan Shi, Shaofen Xu, Haoyu Cheng, Lihong Wu, Janak L. Pathak, Zhichao Zheng
Summary: This review summarizes the regulatory role of non-coding RNAs in the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) and discusses their prospects in clinical bone tissue regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Marcus Lee, Sathya Kannan, Giridharan Muniraj, Vinicius Rosa, Wen Feng Lu, Jerry Y. H. Fuh, Gopu Sriram, Tong Cao
Summary: The role of angiogenesis in health and disease is gaining attention. Two-photon excitation fluorescence microscopy provides high-quality imaging for studying the molecular mechanisms of angiogenesis. Understanding angiogenesis is important for studying disease responses, tissue model development, and therapeutic strategies.
TISSUE ENGINEERING PART B-REVIEWS
(2022)
Review
Dentistry, Oral Surgery & Medicine
Vinicius Rosa, Gopu Sriram, Neville McDonald, Bruno Neves Cavalcanti
Summary: This review discusses various methods used in research and clinical practice for pulp regeneration, including laboratory-based methods, animal studies, and clinical translation. It highlights the advantages, disadvantages, and contributions of these methods to pulp regeneration.
INTERNATIONAL ENDODONTIC JOURNAL
(2022)
Article
Engineering, Biomedical
Craig Jun Kit Wong, Yee Kit Tai, Jasmine Lye Yee Yap, Charlene Hui Hua Fong, Larry Sai Weng Loo, Marek Kukumberg, Jurg Frohlich, Sitong Zhang, Jing Ze Li, Jiong-Wei Wang, Abdul Jalil Rufaihah, Alfredo Franco-Obregon
Summary: Pulsing electromagnetic fields (PEMFs) have been shown to promote myogenesis both in vitro and in vivo, with secretome activation playing a key role. Downwardly-directed magnetic fields elicit stronger secretome responses, particularly in extracellular vesicle secretion. Conditioned media from magnetically-stimulated myoblasts provide a survival advantage over fetal bovine serum.
Article
Dentistry, Oral Surgery & Medicine
Shijia Hu, Giridharan Muniraj, Apurva Mishra, Kanglun Hong, Jing Li Lum, Catherine Hsu Ling Hong, Vinicius Rosa, Gopu Sriram
Summary: The study characterizes the cytotoxicity potential of silver diamine fluoride (SDF) on dental pulp stem cells (DPSC) and gingival equivalents. The results show that SDF can penetrate dentin and induce death of pulp cells, while also disrupting gingival epithelial integrity and causing mucosal corrosion.
Article
Chemistry, Multidisciplinary
Vinicius Rosa, Shruti Vidhawan Agarwalla, Bing Liang Tan, Si Yi Choo, Yu Fan Sim, Freddy Yin Chiang Boey, Sudarshan Anantharaman, Mandeep Singh Duggal, Kai Soo Tan
Summary: The study evaluated the ability of the D-DART to reduce the spread of aerosols generated during dental treatment. The results showed that the D-DART effectively contained and prevented the spread of aerosols generated during deep scaling procedures.
APPLIED SCIENCES-BASEL
(2022)
Article
Orthopedics
Michael Tim-Yun Ong, Sai-Chuen Fu, Sze-Wing Mok, Alfredo Franco-Obregon, Stacy Lok-Sze Yam, Patrick Shu-Hang Yung
Summary: The study aimed to investigate the effect of resistance exercise on myokine levels and muscle atrophy status in ACLR patients with persistent quadriceps muscle atrophy. The results showed that quadriceps muscle atrophy was correlated with muscle weakness and quadriceps strength deficits. Serum brain-derived neurotrophic factor (BDNF) was up-regulated in response to resistance exercise in the non-atrophy group, but a negative fold change was detected in the atrophy group.
ASIA-PACIFIC JOURNAL OF SPORT MEDICINE ARTHROSCOPY REHABILITATION AND TECHNOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Mi-Ah Kim, Vinicius Rosa, Kyung-San Min
Summary: This study investigated the antibacterial efficacy and cytotoxicity of two graphene derivatives - graphene oxide (GO) and reduced graphene oxide (RGO) - against Enterococcus faecalis in biofilms formed on hydroxyapatite discs. GO and RGO had similar antibacterial effects on E. faecalis biofilms. SEM images showed the ability of GO and RGO to disrupt E. faecalis, while GO had significantly higher cytotoxicity than RGO. The results suggest that RGO has the potential to be used as a more effective disinfecting agent than GO.
DENTAL MATERIALS JOURNAL
(2023)
Article
Biochemical Research Methods
Toan V. Phan, Yamin Oo, Khurshid Ahmed, Teerapat Rodboon, Vinicius Rosa, Supansa Yodmuang, Joao N. Ferreira
Summary: Hyposalivation and severe dry mouth syndrome are common complications in HNC patients after radiation therapy. Conventional treatment with sialogogues is limited due to the destruction of the salivary gland's secretory parenchyma and reduced regenerative potential. Adipose mesenchymal stem cells (AdMSC) show promise in addressing this issue.
Article
Dentistry, Oral Surgery & Medicine
Arief Cahyanto, Marcos Vinicius Surmani Martins, Otavio Bianchi, Deepa Perumbodathu Sudhakaran, Nikolaos Sililkas, Sergio Graniero Echeverrigaray, Vinicius Rosa
Summary: This study investigated the effects of graphene oxide (GO) on the reliability and lifetime of PMMA. The results showed that GO improved the fatigue resistance and predicted lifetime of PMMA, but had no significant effect on its Weibull parameters.
Article
Dentistry, Oral Surgery & Medicine
A. Cahyanto, P. Rath, T. X. Teo, S. S. Tong, R. Malhotra, B. N. Cavalcanti, L. Z. Lim, K. S. Min, D. Ho, W. F. Lu, V. Rosa
Summary: This study used Taguchi's methods and genetic algorithms to optimize the properties of calcium silicate cements, achieving personalized treatment for patients' specific needs. By simultaneously analyzing multiple input factors, the study successfully designed cement formulations with predicted properties, which were experimentally validated. The study also demonstrated the bioactivity of the cement in dental pulp stem cells.
JOURNAL OF DENTAL RESEARCH
(2023)
Review
Biotechnology & Applied Microbiology
Alfredo Franco-Obregon, Yee Kit Tai, Kwan Yu Wu, Jan Nikolas Iversen, Craig Jun Kit Wong
Summary: Muscle function is influenced by muscular mitochondrial status, which is a response to physical activity and improves energy production. Different muscle performance is due to variation in contractile-protein isoforms and mitochondrial-energy substrate utilization. Glycolytic muscles generate immediate energy from carbohydrates outside the mitochondria, while oxidative muscles produce energy aerobically from fatty acids. The oxidative muscle phenotype is correlated with metabolic balance and longevity. The activation of the muscle mitochondrial pool through magnetic fields can mimic the oxidative muscle phenotype. Further research on the physiological consequences of magnetic-field therapy is necessary.
BIOENGINEERING-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Jurg Traber, Thomas Wild, Joerg Marotz, Martin C. Berli, Alfredo Franco-Obregon
Summary: This study analyzed the effects of concurrent optical and magnetic stimulation (COMS) therapy on wound healing-related parameters using hyperspectral imaging. The results showed that COMS therapy can improve edema and perfusion, increase tissue oxygenation, and decrease water content, thus providing a positive short-term effect on chronic wounds.
BIOENGINEERING-BASEL
(2023)
Article
Cell Biology
Sharanya Venugobal, Yee Kit Tai, Jorming Goh, Sean Teh, Craig Wong, Ivan Goh, Andrea B. Maier, Brian K. Kennedy, Alfredo Franco-Obregon
Summary: Brief weekly exposure to low energy pulsed electromagnetic fields (PEMFs) has been shown to improve human muscle mitochondrial bioenergetics and attenuate systemic lipotoxicity following anterior cruciate ligament surgical reconstruction. In a study of 101 participants, significant improvements in mobility and body composition were observed after 12 weeks of PEMF intervention, particularly in older participants. Perception of pain was also significantly reduced. These findings suggest that PEMF therapy may have potential clinical benefits for age-associated mobility and metabolic disruptions.
Article
Dentistry, Oral Surgery & Medicine
Shiyao Liu, Ahmed El-Angbawi, Ruidong Ji, Vinicius Rosa, Nick Silikas
Summary: The objective of this study was to investigate the effects of adding graphene to orthodontic fixed retainer adhesives on colour and optical transmission. The results showed that adding graphene significantly darkened the adhesive colour and reduced translucency, but had no adverse effect on degree of conversion. The optical transmission of the adhesive decreases with increasing graphene concentration.
EUROPEAN JOURNAL OF ORAL SCIENCES
(2023)
Review
Engineering, Biomedical
Harish K. Handral, Vaishali P. Natu, Tong Cao, Jerry Y. H. Fuh, Gopu Sriram, Wen F. Lu
BIO-DESIGN AND MANUFACTURING
(2022)