Article
Infectious Diseases
Marta Palombo, Federica Bovo, Stefano Amadesi, Paolo Gaibani
Summary: Limited treatment options contribute to the emergence of antimicrobial resistance, and new therapeutic agents are needed for the treatment of certain pathogens. The combination of antibiotics is an effective strategy to combat multidrug-resistant infections.
Article
Pharmacology & Pharmacy
Joonhyeok Choi, Ahjin Jang, Young Kyung Yoon, Yangmee Kim
Summary: This study designed analogs of PapMA with Trp(18) showing the highest bacterial selectivity against CRAB and low cytotoxicity. PapMA-3 in combination with six selected antibiotics exhibited synergistic antibacterial activity against CRAB, providing a potential therapeutic approach against this pathogen.
Review
Infectious Diseases
Tommaso Lupia, Ilaria De Benedetto, Giacomo Stroffolini, Stefano Di Bella, Simone Mornese Pinna, Verena Zerbato, Barbara Rizzello, Roberta Bosio, Nour Shbaklo, Silvia Corcione, Francesco Giuseppe De Rosa
Summary: Temocillin has shown to be a suitable alternative for treating infections caused by ESBL and KPC-producing bacteria, with advantages such as favorable microbiota profile, low risk of C. difficile infections, and effectiveness against various resistant strains.
Article
Microbiology
Noor Zaidan, J. Patrik Hornak, David Reynoso
Summary: A successful case of treating XDR Acinetobacter baumannii pneumonia and septic shock with a novel antibiotic obtained via expanded access protocol was reported, suggesting that focused research and drug development efforts can mitigate the poor outcomes associated with these infections.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Suying Zhao, Xingyi Qiu, Yiting Tan, Min Xu, Xiaotong Xu, Yulai Ge, Huiling Cao, Min Li, Yuxin Lin, Zhan Zhang, Lei Li, Qian Wu
Summary: This study investigated the clonal lineages, antibiotic resistance profiles, and virulence factors of carbapenem-resistant strains isolated from hospitals. The majority of the isolates belonged to the ST11 type and were grouped into three clones based on PFGE patterns. The study also found that these drug-resistant isolates harbored various antibiotic resistance genes and virulence factors.
CURRENT MICROBIOLOGY
(2022)
Article
Immunology
Qin Wang, Guiyi Liao, Quan Xia, Chaoliang Ge, Handong Ding
Summary: This study investigated the efficacy and adverse events of tigecycline therapy in renal transplant patients with carbapenem-resistant gram-negative bacterial infections. The results showed that tigecycline therapy appeared to be well tolerated in these patients, but attention should be paid to adverse reactions and close monitoring of laboratory tests.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Immunology
Flora Cruz-Lopez, Adrian Martinez-Melendez, Rayo Morfin-Otero, Eduardo Rodriguez-Noriega, Hector J. Maldonado-Garza, Elvira Garza-Gonzalez
Summary: Infections caused by Gram-negative multi-drug resistant (MDR) bacterial species are difficult to treat due to widespread antibiotic resistance. New antibiotics and combinations with existing drugs have shown potential in treating these infections, but there are reports of resistance to these treatments.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Public, Environmental & Occupational Health
Hai-Hui Zhuang, Ying Chen, Qin Hu, Wen-Ming Long, Xiao-Li Wu, Qin Wang, Tian-Tian Xu, Qiang Qu, Yi-Ping Liu, Yi-Wen Xiao, Jian Qu
Summary: This study retrospectively evaluated the clinical and microbiological efficacy, patient mortality, and other associated factors of ceftazidime/avibactam (CAZ/AVI)-based regimens for carbapenem-resistant Gram-negative bacteria (CR-GNB). The results showed that CAZ/AVI had good clinical and microbiological efficacy in treating CR-GNB infection, but renal dysfunction and the adjusted dose of CAZ/AVI could affect efficacy. Rating: 8 out of 10.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2023)
Article
Immunology
Mira El Masri, Nisrine Haddad, Therese Saad, Nesrine A. Rizk, Ramia Zakhour, Souha S. Kanj, Rony M. Zeenny
Summary: This study evaluated the use of carbapenem before and after the implementation of an antimicrobial stewardship-led carbapenem-sparing strategy at a tertiary care center in Lebanon. The results showed an increase in the number of pharmacist interventions and their appropriateness in carbapenem therapy, while improvements in physician-prescribing patterns may require more awareness and education.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Min Hao, Yang Yang, Yan Guo, Shi Wu, Fupin Hu, Xiaohua Qin
Summary: Combination therapies with colistin sulfate are recommended for the treatment of carbapenem-resistant Gram-negative bacilli (CR-GNB) infections, as they have better clinical efficacy compared to colistin sulfate monotherapy.
Article
Microbiology
Hea-Jin Jung, Matthew T. Sorbara, Eric G. Pamer
Summary: This study shows that translocation and assembly module (TAM) mediates antibiotic and antimicrobial peptide resistance during host colonization and infection with highly antibiotic-resistant Klebsiella pneumoniae (CR-Kp). Loss of TAM impairs envelope biogenesis and increases the sensitivity of CR-Kp to large antimicrobials under stress conditions. Stress-induced sensitization enhances clearance of CR-Kp from the gut lumen and infection sites, offering a potential target for therapeutic agents to enhance permeability and effectiveness of existing and potential antibiotics.
Article
Infectious Diseases
Jian Qu, Ting-Ting Qi, Qiang Qu, Wen-Ming Long, Ying Chen, Yue Luo, Ying Wang
Summary: This study explores the efficacy and safety of Polymyxin B (PMB)-based regimens for treating carbapenem-resistant Gram-negative bacteria (CR-GNB) infections and analyzes factors influencing their effectiveness and mortality. The results show that PMB can be relatively safe and effective in treating CR-GNB infections in critically ill patients, but should be used according to guidelines for early administration, loading administration, and adequate administration.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Medicine, General & Internal
H. Tootla, J. Copelyn, A. Botha, A. J. Brink, B. Eley
Summary: Newer beta-lactam-inhibitor combination (BLIC) antibiotics are now available in South Africa for treating carbapenem-resistant infections, but challenges such as access to susceptibility testing, procurement process, cost, and decision-making complexity have been faced in using these lifesaving antibiotics.
SAMJ SOUTH AFRICAN MEDICAL JOURNAL
(2021)
Review
Medicine, General & Internal
Erlangga Yusuf, Hannelore I. Bax, Nelianne J. Verkaik, Mireille van Westreenen
Summary: Infections in the ICU are often caused by Gram-negative bacteria, which can become resistant to traditional antibiotics, limiting treatment options. In recent years, FDA has approved new antibiotics with predominant activities against Gram-negative bacteria, providing more choices for treatment in specific circumstances.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Infectious Diseases
Jose Raul Nieto-Saucedo, Luis Esau Lopez-Jacome, Rafael Franco-Cendejas, Claudia Adriana Colin-Castro, Melissa Hernandez-Duran, Luis Raul Rivera-Garay, Karina Senyase Zamarripa-Martinez, Juan Luis Mosqueda-Gomez
Summary: The prevalence of carbapenem-resistant Gram-negative bacilli (CR-GNB) and the frequency of carbapenemase-encoding genes were evaluated in a tertiary referral center in El Bajio, Mexico. Among 508 Gram-negative bacilli, 37 were carbapenem-resistant (7.3%), and only 15 (40.5%) of them carried carbapenemase-encoding genes.
Article
Immunology
Brian M. Forde, Haakon Bergh, Thom Cuddihy, Krispin Hajkowicz, Trish Hurst, E. Geoffrey Playford, Belinda C. Henderson, Naomi Runnegar, Julia Clark, Amy Jennison, Susan Moss, Anna Hume, Hugo Leroux, Scott A. Beatson, David L. Paterson, Patrick N. A. Harris
Summary: This study implemented a program of routine sequencing of multi-drug resistant organisms and used custom analysis pipeline to automate reporting and support infection control interventions. The results showed the identification of transmission events within three hospitals, highlighting the feasibility of routine WGS for MDR pathogens.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Yu Takahashi, Hideaki Wakita, Takuma Ishihara, Hideto Okazaki, Akihiro Ito, Mitsunaga Iwata, Shigeru Sonoda, Yohei Doi
Summary: Healthcare-associated COVID-19 among vulnerable patients leads to disproportionate morbidity and mortality. Early pharmacologic intervention may reduce negative sequelae and improve survival in such settings. This study aimed to describe outcome of patients with healthcare-associated COVID-19 who received early short-course remdesivir therapy.
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Hiroyuki Naruse, Yohei Doi, Mitsunaga Iwata, Kiyohito Ishikawa
Summary: During the COVID-19 pandemic, Japan's early return-to-work (RTW) program for healthcare workers (HCWs) who have been in close contact with COVID-19 cases was found to be a reasonable strategy. HCWs eligible for the program had received the third dose of a COVID-19 mRNA vaccine, tested negative for COVID-19 before each work shift, and were difficult to replace. Out of 256 HCWs identified as close contacts, 37 secondary cases were detected, and 141 HCWs (55%) participated in the early RTW program, with no reported infection clusters.
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Yohei Doi, Takuma Ishihara, Sumi Banno, Masahiko Ando, Masashi Kondo
Summary: This study investigated the use of favipiravir in COVID-19 patients between February 2020 and December 2021. The results showed that favipiravir was effective and well tolerated in treating COVID-19 patients.
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2023)
Article
Pathology
Jill Parkes-smith, Haakon Bergh, Patrick N. A. Harris
Summary: Staphylococcus aureus bacteraemia has high morbidity and mortality, and timely use of effective antibiotics is crucial. Molecular diagnostics can shorten the time from blood culture positivity to identification of MRSA or MSSA.
Review
Infectious Diseases
Elda Righi, Luigia Scudeller, Massimo Mirandola, Alessandro Visentin, Nico T. Mutters, Marco Meroi, Anna Schwabe, Anna Erbogasto, Gianluca Vantini, Elizabeth L. A. Cross, Maddalena Giannella, Xavier Guirao, Evelina Tacconelli
Summary: This study conducted a systematic review and meta-analysis and found that patients colonised with extended-spectrum cephalosporin-resistant Enterobacterales (ESCR-E) before surgery are more likely to develop postoperative infections. Preoperative screening for ESCR-E in adult inpatients may help reduce the incidence of surgical site infections.
INFECTIOUS DISEASES AND THERAPY
(2023)
Article
Microbiology
Clement Kin-Ming Tsui, Fatma Ben Abid, Khalil Al Ismail, Christi Lee McElheny, Muna Al Maslamani, Ali S. Omrani, Yohei Doi
Summary: The emergence of carbapenem-resistant, hypervirulent Klebsiella pneumoniae is a new threat to health care. We studied the molecular epidemiology of carbapenem-resistant Klebsiella pneumoniae isolates in Qatar using whole-genome sequence data. We also characterized the prevalence and genetic basis of hypervirulent phenotypes and established the virulence potential using a Galleria mellonella model.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Adam G. G. Stewart, Jason A. A. Roberts, Brian M. M. Forde, Haakon Bergh, Timothy J. J. Kidd, Hugh Wright, Patrick N. A. Harris
Summary: Elizabethkingia meningoseptica is a rare multidrug-resistant gram-negative bacterium that primarily causes infections in vulnerable hosts. It can cause severe sepsis and complicated infections, and there is limited data on suitable therapeutic options. This study presents a case of prolonged bloodstream and central nervous system infection due to E. meningoseptica, treated with dose-optimized combination antibiotic therapy, but showed evidence of microbiological and clinical failure.
MICROBIAL DRUG RESISTANCE
(2023)
Article
Microbiology
Gayatri Shankar Chilambi, Yu-Hao Wang, Nathan R. Wallace, Chetachukwu Obiwuma, Kirsten M. Evans, Yanhong Li, Menna-Allah W. Shalaby, Daniel P. Flaherty, Ryan K. Shields, Yohei Doi, Daria Van Tyne
Summary: This study identified a mutant strain of Enterococcus faecalis that is hypersusceptible to aminoglycoside antibiotics due to disruption of alpha-carbonic anhydrase (alpha-CA). Disruption of alpha-CA increased proton motive force and membrane permeability, leading to increased gentamicin uptake in E. faecalis. Additionally, an FDA-approved alpha-CA inhibitor sensitized various E. faecalis strains to gentamicin.
MICROBIOLOGY SPECTRUM
(2023)
Editorial Material
Infectious Diseases
Luke S. P. Moore, Maria Virginia Villegas, Eric Wenzler, Timothy M. Rawson, Rita O. Oladele, Yohei Doi, Anucha Apisarnthanarak
Summary: Despite variable utilization of rapid diagnostic tests (RDTs) in antimicrobial stewardship programs (ASPs) across different income countries, a value framework has been proposed to define the benefits of RDTs in ASPs, separate from per-patient benefits. Effective implementation is key to realizing the value of RDTs within ASPs, and actionable advice for choosing an RDT has been proposed by infectious disease experts from various countries. These experts also advocate for the inclusion of RDTs in the World Health Organization Model List of essential in vitro diagnostics and in the iterative development of national action plans.
INFECTIOUS DISEASES AND THERAPY
(2023)
Article
Infectious Diseases
Jinnethe Reyes, Lauren Komarow, Liang Chen, Lizhao Ge, Blake M. Hanson, Eric Cober, Erica Herc, Thamer Alenazi, Keith S. Kaye, Julia Garcia-Diaz, Lanjuan Li, Souha S. Kanj, Zhengyin Liu, Jose M. Onate, Robert A. Salata, Kalisvar Marimuthu, Hainv Gao, Zhiyong Zong, Sandra L. Valderrama-Beltran, Yunsong Yu, Paul Tambyah, Gregory Weston, Soraya Salcedo, Lillian M. Abbo, Qing Xie, Karen Ordonez, Minggui Wang, Martin E. Stryjewski, Jose M. Munita, David L. Paterson, Scott Evans, Carol Hill, Keri Baum, Robert A. Bonomo, Barry N. Kreiswirth, Maria Virginia Villegas, Robin Patel, Cesar A. Arias, Henry F. Chambers, Vance G. Fowler Jr, Yohei Doi, David van Duin, Michael J. Satlin
Summary: This study investigated the characteristics and outcomes of carbapenem-resistant Pseudomonas aeruginosa (CRPA) infections and the distribution and clinical significance of carbapenemases. The results showed that carbapenemase-producing CRPA infections were associated with increased 30-day mortality, and the prevalence of carbapenemase genes varied by region. These findings highlight the therapeutic challenges posed by these carbapenem-resistant organisms.
Article
Immunology
Michael J. Satlin, David van Duin, Pranita D. Tamma, Thomas P. Lodise, Daria Van Tyne, Keith A. Rodvold, Nadine Rouphael, Scott R. Evans, Vance G. Fowler, Toshimitsu Hamasaki, Robin Patel, Lauren Komarow, Keri Baum, Maria Souli, Nyssa Schwager, Robert A. Bonomo, Yohei Doi
Summary: Addressing antibacterial-resistant gram-negative infections is a priority of the Antibacterial Resistance Leadership Group. The focus of investigation has evolved from elucidating the epidemiology of these infections to improving patient care through clinical trials of optimizing pharmacokinetics and of novel therapies.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Medicine, General & Internal
Natalie Davidson, Felicity Edwards, Patrick N. A. Harris, Kevin B. Laupland
Summary: This study aimed to determine the incidence and risk factors for developing Vibrio species bloodstream infections and compare differences between different species. The study found an incidence rate of 1.2 cases/1 million person-years for Vibrio infections in Australia, with the highest incidence occurring in the summer months and in the tropical north. Increasing age, male sex, and multiple comorbidities were identified as risk factors. Vibrio vulnificus was the most frequently isolated pathogen and associated with the most severe disease. The overall case fatality rate was 19%.
INTERNAL MEDICINE JOURNAL
(2023)
Article
Medicine, General & Internal
Adam G. Stewart, Claire Heney, David L. Paterson, Patrick N. A. Harris, Felicity Edwards, Kevin B. Laupland
Summary: A 20-year study in Queensland, Australia, found that Scedosporium and Lomentospora species, which are environmental moulds, rarely cause bloodstream infection in immunocompromised hosts. Among 22 incident episodes of Scedosporium and Lomentospora species bloodstream infection, 18 were caused by Lomentospora prolificans, 3 by Scedosporium apiospermum complex, and 1 by a nonspeciated Scedosporium species. The overall mortality rate for these patients was high, with 81% dying during their index admission and all-cause mortality at 30, 90, and 365 days being 73%, 82%, and 91% respectively. In addition, all 20 patients with haematological malignancy died within 365 days of follow-up, with a median time to death of 9 days following the diagnosis of bloodstream infection.
INTERNAL MEDICINE JOURNAL
(2023)
Article
Infectious Diseases
Adam G. Stewart, Michelle J. Bauer, Dominika Butkiewicz, Alexandra Hinton, Andrew Henderson, Patrick N. A. Harris, David L. Paterson
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)