Article
Biochemistry & Molecular Biology
Bin-Bin Yang, Shi-Yuan Miao, Yu-Jie Lu, Sui-Sui Wang, Zheng-Yan Wang, Ya-Ru Zhao
Summary: In this study, the roles of Methoprene-tolerant (Met) and Kruppel-homolog 1 (Kr-h1) in the reproduction of Liposcelis entomophila were investigated. Knockdown of Met and Kr-h1 resulted in decreased vitellogenesis and ovarian development, leading to a decline in fecundity. Moreover, the expression of other hormone factors, such as FoxO, was also affected. These findings highlight the importance of JH signaling in female reproduction of psocids and suggest that RNAi-mediated knockdown of Met or Kr-h1 could be a potential pest control strategy.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jimena Leyria, Ian Orchard, Angela B. Lange
Summary: In adult females of several insect species, juvenile hormones (JHs) play a role as gonadotrophic hormones, regulating egg production and successful hatching through the JHSB(3) signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Entomology
Hui Han, ZhaoYang Feng, ShiPeng Han, Jie Chen, Da Wang, YunZhuan He
Summary: This study identified and analyzed the Met and Kr-h1 genes in Harmonia axyridis and found that they play important roles in female reproduction. Knockdown of these genes significantly reduced yolk protein deposition and fecundity. The findings provide a basis for the application or control of H. axyridis.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shuang Guo, Qing-Wen Wu, Zhong Tian, Li Zhu, Kirst King-Jones, Fen Zhu, Xiao-Ping Wang, Wen Liu
Summary: This study revealed that JH-Methoprene-tolerant signaling regulates reproductive plasticity in female cabbage beetles, with Kr-h1 playing a key role in this process. When Kr-h1 function is disrupted, reproductive development is blocked and diapause-like traits are induced. Kr-h1 acts both as an activator and repressor, depending on the downstream genes involved in reproduction or diapause pathways.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
H. -Y. Ma, Y. -Y. Li, L. Li, Y. Tan, B. -P. Pang
Summary: The study discovered that topical application of the JH analogue methoprene induced the expression of GdMet in 3-day-old and 5-day-old adults of Galeruca daurica. Silencing GdMet through RNAi inhibited the expression of JHBP, JHE, Kr-h1, and Vg, while promoting FAS2 expression, leading to enhanced lipid accumulation and fat body development, ultimately inducing the adults into diapause ahead of schedule.
INSECT MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Edmundo Gassias, Annick Maria, Philippe Couzi, Elodie Demondion, Nicolas Durand, Francoise Bozzolan, Paleo Aguilar, Stephane Debernard
Summary: This study investigates the effects of juvenile hormone on the development and secretory activity of the accessory glands in Agrotis ipsilon, revealing the crucial role of the JH-Met-Kr-h1 signaling pathway in this process.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Yu-Pu Jing, Xinpeng Wen, Lunjie Li, Shanjing Zhang, Ci Zhang, Shutang Zhou
Summary: Using the migratory locust as a model system, the study found that juvenile hormone promotes phosphorylation of Vitellogenin receptor, which is essential for Vitellogenin uptake and recycling. This phosphorylation regulation is evolutionarily conserved in other insects as well.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Entomology
Xue Pan, Yanfang Pei, Cuici Zhang, Yaling Huang, Ling Chen, Liqiong Wei, Chuanren Li, Xiaolin Dong, Xiang Chen
Summary: This study reveals the role of the insulin signaling pathway in the reproductive process of Spodoptera litura. Knockdown of the SlInR gene impairs ovarian development and decreases fecundity. In addition, insulin signaling is involved in regulating various physiological functions in insects, including energy metabolism.
Review
Physiology
Xiaoshuai Zhang, Sheng Li, Suning Liu
Summary: JH, produced by the endocrine gland corpus allatum, plays a critical role in regulating developmental and physiological processes in insects. The fruit fly Drosophila melanogaster is a key model for studying JH.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biology
Atul Pandey, Guy Bloch
Summary: Dominance hierarchies are common in animals, but little is known about how genes influence dominance rank, especially among female insects. This study identified the gene Kr-h1 as playing a key role in regulating dominance behavior in social bees.
Article
Biochemistry & Molecular Biology
Yan-Xue Li, Di Wang, Wen-Li Zhao, Jun-Ying Zhang, Xin-Le Kang, Yan-Li Li, Xiao-Fan Zhao
Summary: This study reveals that phosphorylation of Met1 protein at Thr393 by JH is crucial for promoting its binding to JHRE to regulate Kr-h1 transcription, and plays a key role in preventing Met1-Met1 association.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Youhei Suzuki, Takahiro Shiotsuki, Akiya Jouraku, Ken Miura, Chieka Minakuchi
Summary: In thrips metamorphosis, the adult specifier gene E93 starts to increase in expression at the end of the larval stage or in the propupal stage, coinciding with decreased levels of Kruppel homolog 1 and broad genes. Exogenous treatment with a JH mimic can prevent pupal-adult transition and repress the expression of E93. Comparing the expression profiles of key genes among different insect species suggests similarities between thrips' propupal and pupal stages with holometabolous species.
Article
Biochemistry & Molecular Biology
Michiel Holtof, Joachim Van Lommel, Marijke Gijbels, Elfie Dekempeneer, Bart Nicolai, Jozef Vanden Broeck, Elisabeth Marchal
Summary: Africa and Asia are currently facing the worst desert locust plague in decades, with favorable environmental conditions due to exceptionally high rainfall. Research shows that components of the juvenile hormone signaling pathway play a crucial role in male locust reproductive physiology, indicating their potential as molecular targets for pest control.
Article
Entomology
Zhi-Ming Yang, Tian-Yu Lu, Yong Wu, Na Yu, Guang-Ming Xu, Qian-Qian Han, Ze-Wen Liu
Summary: The study revealed the presence of two VgRs, VgR-1 and VgR-2, in the pond wolf spider, with VgR-1 playing a crucial role in oviposition by having higher expression levels in ovaries compared to VgR-2. Vg-1 and Vg-2 were identified as potential ligands of VgR-1, and the expression of VgRs and Vgs were regulated by juvenile hormones, indicating their essential role in vitellogenesis in spiders.
Review
Behavioral Sciences
Paleo Aguilar, Thomas Bourgeois, Annick Maria, Philippe Couzi, Elodie Demondion, Francoise Bozzolan, Edmundo Gassias, Evan Force, Stephane Debernard
Summary: In insects, juvenile hormone (JH) plays a critical role in the development of male reproductive behavior. The expression of JH receptors and JH-inducible transcription factor within the primary olfactory centers correlates with the behavioral responsiveness to sex pheromone. JH acts through the Met-Kr-h1 signaling pathway to promote the processing of pheromone information and the display of sexual behavior in synchronization with fertility.
HORMONES AND BEHAVIOR
(2023)
Article
Entomology
Qiao Gao, Bei Li, Bing-Xin Wei, Wen Liu, Pan Wang, Jia-Lu Wang, Xing-Miao Zhou, Xiao-Ping Wang
Summary: This study revealed that photoperiod regulates male IRS development in H. axyridis through a conserved Met-dependent JH signaling pathway. The results indicate the importance of JH in IRS development and how Met knockdown can affect this process in male ladybeetles.
Article
Agronomy
Qiao Gao, Bei Li, Zhong Tian, Arnold De Loof, Jia-Lu Wang, Xiao-Ping Wang, Wen Liu
Summary: Our study reveals that in the Asian lady beetle Harmonia axyridis, the absence of juvenile hormone (JH) triggers reproductive diapause, as indicated by JH measurements and the knockdown of the intracellular JH receptor methoprene-tolerant (Met). Furthermore, we demonstrate that JH biosynthesis, rather than the degradation pathway, determines the reduction of JH titer in diapausing females.
PEST MANAGEMENT SCIENCE
(2022)
Article
Environmental Sciences
Wanqiang Wang, Wenzheng Tang, Xiaoping Wang, Wen Liu, Fen Zhu
Summary: The study found that regulating the expression levels of CPA1, GST2, and CYTb-c1 genes through RNA interference can reduce the accumulation of Pb in the larvae of houseflies. These proteins play crucial roles in Pb transport and detoxification in M. domestica larvae.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Agronomy
Zhong Tian, Shuang Guo, Fen Zhu, Wen Liu, Xiao-Ping Wang
Summary: This study showed that disrupting COPII complex assembly can effectively inhibit the growth and development of Colaphellus bowringi adults, highlighting the potential of targeting the COPII complex as an RNAi-based approach for pest control in cruciferous vegetables. Silencing the COPII assembly genes not only suppressed ovarian growth and yolk deposition but also affected feeding behavior and increased mortality. These effects were associated with the down-regulation of insulin/mTOR-associated nutritional pathways and repression of juvenile hormone and ecdysone signaling pathways.
PEST MANAGEMENT SCIENCE
(2022)
Article
Microbiology
Zhendong Huang, Li Zhu, Jia Lv, Zhanxu Pu, Lipin Zhang, Guoqing Chen, Xiurong Hu, Zhenyu Zhang, Hongyu Zhang
Summary: This study investigated the influence of different food sources on the gut microbiota diversity and biological parameters of the multicolored Asian lady beetle. The results showed that different diets can affect the gut microbiota composition and diversity, as well as the biological parameters of the lady beetle.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Cell Biology
Zijie Huang, Zhong Tian, Yulian Zhao, Fen Zhu, Wen Liu, Xiaoping Wang
Summary: This study investigated the role of the MAPK signaling pathway in ovarian growth and oviposition in the cabbage beetle and found that ERK and P38 pathways are important for female survival and reproduction. Additionally, ERK pathway possibly regulates ecdysone biosynthesis and P38 pathway may be involved in germline stem cell development and differentiation.
Editorial Material
Physiology
Julie A. Reynolds, Wen Liu, Kunihiro Shiomi
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lian Chen, Zhong Tian, Jin Hu, Xiao-Yun Wang, Man-Qun Wang, Wen Lu, Xiao-Ping Wang, Xia-Lin Zheng
Summary: This study focuses on the role of sex pheromone-binding proteins (PBPs) in Lepidopteran insects. Two novel PBPs, PflaPBP1 and PflaPBP2, were identified in Phauda flammans, a diurnal moth species. PflaPBP1 has a stronger affinity for female sex pheromones, while PflaPBP2 only binds to a specific sex pheromone component. These findings provide insights into the molecular mechanism of sex pheromone perception and may contribute to pest control strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jia-Xu Li, Zhong Tian, Xing-Feng Liu, Bei Li, Hao-Min An, Colin S. Brent, Jia-Lu Wang, Xiao-Ping Wang, Wen Liu
Summary: Many animals exhibit plasticity of body colour in response to environmental changes, and carotenoids significantly contribute to this flexibility. However, the molecular mechanisms of carotenoid expression regulation by environmental cues are still largely unknown. This study used the ladybird Harmonia axyridis as a model and found that the differential accumulation of carotenoids resulted in a redder elytra coloration in females under long-day conditions compared to those under short-day conditions. The researchers identified the juvenile hormone (JH) receptor-mediated canonical pathway as the regulator of carotenoid deposition through exogenous hormone application and gene knockdown experiments. Furthermore, they characterized the SCRB10 gene as the carotenoid transporter responding to JH signaling and regulating the elytra coloration plasticity.
Article
Cell Biology
Sakhawat Shah, Karam Khamis Elgizawy, Chun-Mei Shi, Hucheng Yao, Wen-Han Yan, Yu Li, Xiao-Ping Wang, Gang Wu, Feng-Lian Yang
Summary: This study found that Diallyl trisulfide (DAT), a component of garlic essential oil, can decrease the male fertility of Sitotroga cerealella. The results showed that DAT significantly decreased the dimorphic sperm number and caused abnormal mitochondrial structures of the sperm. It also reduced lipid homeostasis, ATP contents, total sperm motility, and other sperm parameters. This study reported, for the first time, that DAT impairs energy metabolism, inhibits dimorphic spermatogenesis, and decreases sperm motility, leading to adult-male infertility.
Article
Cell Biology
Sakhawat Shah, Karam Khamis Elgizawy, Meng-Ya Wu, Hucheng Yao, Wen-Han Yan, Yu Li, Xiao-Ping Wang, Gang Wu, Feng-Lian Yang
Summary: Essential oils from plants, particularly garlic essential oil, containing Diallyl trisulfide (DAT) have been found to have negative effects on insects, specifically impacting their sperm function and fertility. The study demonstrates that DAT impairs the testis proteasome, leading to male infertility characterized by oligoasthenoteratospermia in Sitotroga cerealella.
Article
Agronomy
Fan Yang, Pan Wang, Min Zheng, Xiao-Yu Hou, Li-Lin Zhou, Yong Wang, Sheng-Yun Si, Xiao-Ping Wang, Jason W. Chapman, Yu-Meng Wang, Gao Hu
Summary: The research on Plutella xylostella in central China reveals that temperature fluctuations affect the migratory propensity and reproduction of the insects. The study sheds light on the physiological and behavioral factors underlying mass migration in P. xylostella, showing that exposure to increased temperature increases their migration propensity at the cost of reproductive output.
PEST MANAGEMENT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Sang Hyuck Lee, Mi Ae Kim, Young Chang Sohn
Summary: This study characterized the CCAP signaling system in Pacific abalone and revealed its relationship with the calcium/PKC and cAMP/PKA signal transduction pathways. It provides new insights into the evolutionary origins of the NPS and OT/VP signaling systems in protostomes.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Hanna Scheuffele, Erica V. Todd, John A. Donald, Timothy D. Clark
Summary: Global warming is causing an increase in extreme weather events, affecting ecosystems and the behavior of ectotherms. More research is needed to understand the impact of diurnal thermal variability and improve climate change predictions.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Jaakko Haverinen, Ahmed Badr, Markus Eskelinen, Matti Vornanen
Summary: The acclimatization of certain ectothermic vertebrates to winter conditions involves reduced energy consumption, achieved through decreased movement activity, depression of cellular functions, or switching to anaerobic energy production. This study on crucian carp showed that winter-acclimatized fish exhibited lower metabolic rates and a shift to anaerobic energy production during anoxia. Winter dormancy in crucian carp is achieved through active reduction of energy consumption, a slowed metabolic rate, and a direct response to oxygen absence.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Maria Cruz Sueiro, Cynthia A. Awruch, Gustavo M. Somoza, Walter S. Svagelj, Maria G. Palacios
Summary: According to life-history theory, there might be different immunological strategies between reproductive and non-reproductive periods due to limited resources. Our study on two sympatric marine fish species, rockfish and sandperch, found that reproductive individuals showed lower immune function, but higher levels of natural antibodies in rockfish females, compared to their non-reproductive counterparts. On the other hand, reproductively active sandperch males showed lower levels of natural antibodies and higher neutrophil to lymphocyte ratio and spleen index compared to non-reproductive males. The study highlights the species-specific patterns of immunity and the potential influence of resource limitation and abiotic factors on immune trade-offs in fish.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
James B. Cummins, Dane A. Crossley II
Summary: This study focuses on cardiovascular development in altricial bird species, which has been less studied compared to precocial species. The researchers investigated the cholinergic and adrenergic receptor mediated control of arterial blood pressure and heart rate in the neotropic cormorant. The findings suggest that both cholinergic and adrenergic tone play a role in cardiovascular regulation in embryonic birds.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Ru Xia, Hong-Kang Liu, Xi-Feng Liu, Xin Deng, Chuan-Jie Qin, Yuan-Fa He, Shi-Mei Lin, Yong-Jun Chen
Summary: This study cloned the genes encoding glucokinase and glucose-6-phosphatase catalytic subunit in largemouth bass, revealing their distribution and transcriptional regulation. The results showed that the functions of G6pc and Gck in LMB were highly conserved in evolution, and the interconversion of glucose and G6P in the liver was well regulated at the transcript level under high starch diet, but a futile cycle was induced after a glucose load.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)