Article
Environmental Sciences
Ricardo Alves Bernardo, Jean Carlos Pereira Sousa, Matheus Gallimberti, Fernando Barbosa Junior, Boniek Gontijo Vaz, Andrea Rodrigues Chaves
Summary: Concerns over human health risks due to the use of bisphenol A in thermal papers have led to its replacement by bisphenol S. Analyzing bisphenols typically involves laborious and time-consuming steps, but a new paper spray ionization mass spectrometry (PSI-MS) method offers a faster and efficient alternative. The study found that bisphenol S concentrations in BPA-free thermal papers ranged from 1.36 to 6.77 mu g g(-1), while BPA concentrations in all samples of thermal paper ranged from 6.56 to 16.4 mu g g(-1).
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Youngju Kal, Sangwon Cha
Summary: Paper spray ionization (PSI) is an extractive ambient ionization technique for mass spectrometry, which can achieve the separation and detection of lipid mixtures. The study found that differential transport of lipid classes occurs during PSI, depending on the polarity of the spraying solvent and the method of application.
Article
Chemistry, Multidisciplinary
Thi Minh Hoa Nguyen, Woo-Young Song, Tae-Young Kim
Summary: This study systematically evaluated the factors affecting the ionization efficiency of paper spray ionization (PSI) and classified the observed paper spray plume into three modes. The rim-jet mode exhibited the highest ionization efficiency among the three modes. The charge density of the droplets generated by paper spray, influenced by the electric field and solvent supply rate, was found to be the main parameter determining the spray mode.
FRONTIERS IN CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Sung Jun An, Youngjoo Kal, Minjoo Jeong, Sumin Kang, Byeongho Kwak, Hyunsoo Kim, Shihyeon Ryu, Sangwon Cha
Summary: In this study, droplet PSI MS utilizing Teslin(R) as a substrate was developed for protein analysis, demonstrating superior sensitivity compared to conventional PSI MS. The method also allowed real-time observation of protein alterations.
Article
Biochemistry & Molecular Biology
Casey A. Chamberlain, Marguerite Hatch, Timothy J. Garrett
Summary: This study introduces a novel application of PSI-MS for the analysis of bacterial EVs for the first time. The isolation and purification of EVs from bacterial culture supernatant using EV-specific affinity chromatography, characterization through nanoparticle tracking analysis, and identification of EV-derived metabolites, lipids, and peptides through PSI-MS represent a pioneering approach in the field of MS-based EV analysis.
Article
Chemistry, Applied
Fabio Mazzotti, Lucia Bartella, Ines Rosita Talarico, Anna Napoli, Leonardo Di Donna
Summary: A fast and accurate methodology has been developed for quantifying the most abundant flavanone glycosides in citrus beverages using paper spray mass spectrometry. The experimental results demonstrate the method's high specificity and sensitivity, making it suitable for practical applications. The comparison of real sample analysis with conventional chromatographic methods confirmed the robustness of the proposed approach.
Article
Chemistry, Analytical
Ping Guo, Ke Min, Wei Luo, Si Huang, Zihui Yang, Ming Ma, Shubin Liu, Zhengfa Fang, Bo Chen, Han Zuilhof
Summary: Ambient ionization of glycans can be achieved efficiently by spraying from a paper surface impregnated with alkali metal salt. This method allows for the formation of abundant alkali metal adduct ions from monosaccharides, oligosaccharides, and ring glycans, resulting in simple and clean mass spectra. The approach is specific for glycans and has been successfully applied in the determination of glycans and glycoconjugates in various biological matrices.
Article
Chemistry, Multidisciplinary
Wangmin Hu, Tianyi Li, Yulei Yang, Shanshan Jia, Mei Zhang
Summary: This study presents a derivative-free and ion mobility-free paper spray tandem mass spectrometry method for rapid analysis of mono- and disaccharides. By calculating the R-value, the species and conformation of simple saccharides can be easily differentiated. The method has potential applications in clinical sample analysis.
CHINESE CHEMICAL LETTERS
(2022)
Article
Biochemical Research Methods
Orobola E. Olajide, Yuyan Yi, Jingyi Zheng, Ahmed M. Hamid
Summary: Determining bacterial identity at the strain level using LC-IM-MS/MS is effective in distinguishing E. coli strains, with prediction rates of 96.1% and 100% for negative and positive ion information, respectively. The PS-IM-MS/MS technique, a faster method, achieved prediction rates of 62.5% and 73.5% in negative and positive ion modes, respectively. The fusion of negative and positive ion data increased the classification rates to 80.5%.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Biochemistry & Molecular Biology
Dongning Song, Song Yuan, Caiyu Zhang, Lin Luan, Yang Liu, Qingsheng Zhang
Summary: Estrogens found in personal care products have negative effects on consumers. The conventional methods for detecting these compounds, such as HPLC and LC-MS, require time-consuming sample pretreatment and analysis. However, the paper-spray ionization mass spectrometry (PSI-MS) method developed in this study allows for quick and efficient determination of estradiol, estriol, and ethinyloestradiol in cosmetics. This method, which involves chemical derivatization, showed excellent linearity, high recovery rates, and significantly improved limits of detection compared to non-derivative PSI-MS techniques.
Article
Chemistry, Analytical
Vincen Wu, Jocelyn Tillner, Emrys Jones, James S. McKenzie, Dipa Gurung, Anna Mroz, Liam Poynter, Daniel Simon, Cristina Grau, Xavier Altafaj, Marc-Emmanuel Dumas, Ian Gilmore, Josephine Bunch, Zoltan Takats
Summary: This study examines the suitability of desorption electro-flow focusing ionization (DEFFI) for mass spectrometry imaging (MSI) of biological tissue, comparing its performance to the traditional desorption electrospray ionization (DESI). The flow focusing mechanism in DESI offers advantages such as a rotationally symmetric electrospray jet, higher intensity, controllable parameters, and better portability. The optimized parameters for DEFFI result in similar images to DESI but with better sensitivity.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Joao Victor Coelho Pimenta, Rodinei Augusti, Adao Aparecido Sabino
Summary: PS-MS is used to investigate the on-surface oxidation of alcohols catalyzed by ruthenium salts, revealing the crucial roles of RuCl3 and TBHP in the process. UV radiation and heating can accelerate the oxidation reaction, offering the possibility of using PS-MS to study organic catalytic reactions and detect unstable intermediates.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Biochemical Research Methods
Chaozi Liu, Yuze Li, Caiqiao Xiong, Zongxiu Nie
Summary: The capillary-paper spray ion source allows for sample separation and enables rapid and sensitive analysis of biofluids. By absorbing proteins and macromolecules, it reduces matrix effects and improves the detection of small molecules. Compared to traditional methods, the CPS method shows improved spray stability, prolonged analysis duration, and enhanced signal intensities, indicating its potential for clinical use.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Physics, Atomic, Molecular & Chemical
Joao Victor Coelho Pimenta, Adao Aparecido Sabino, Rodinei Augusti
Summary: The study investigates the on-surface Povarov reaction using paper spray ionization mass spectrometry and explores the reactivity of different reactants. The results demonstrate that paper spray ionization mass spectrometry can be widely employed to investigate similar on-surface organic reactions.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2022)
Article
Chemistry, Applied
Qiuyu Lin, Jiannan Sun, Yuanchao Wang, Meiying Ye, Heyong Cheng
Summary: A highly sensitive and high-throughput method for aldehyde analysis in food has been developed, which does not require sample pretreatment. This method allows direct quantification of four aldehydes in various food matrices and offers the advantages of simplicity and high efficiency.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Endocrinology & Metabolism
A. L. O'Kell, T. J. Garrett, C. Wasserfall, M. A. Atkinson
Article
Chemistry, Analytical
Vanessa Y. Rubio, Joy G. Cagmat, Gary P. Wang, Richard A. Yost, Timothy J. Garrett
ANALYTICAL CHEMISTRY
(2020)
Article
Biochemical Research Methods
Frederico G. Pinto, Iqbal Mahmud, Taylor A. Harmon, Vanessa Y. Rubio, Timothy J. Garrett
JOURNAL OF PROTEOME RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Laura Patton, Nan Li, Timothy J. Garrett, J. Lauren Ruoss, Jordan T. Russell, Diomel de la Cruz, Catalina Bazacliu, Richard A. Polin, Eric W. Triplett, Josef Neu
Article
Biochemistry & Molecular Biology
Casey A. Chamberlain, Marguerite Hatch, Timothy J. Garrett
Review
Biochemistry & Molecular Biology
Ayon Bhattacharya, Rani Ashouri, Madison Fangman, Alexandra Mazur, Timothy Garett, Sylvain Dore
Summary: Soluble receptors, formed through cleavage from membrane-bound counterparts, play key roles in inflammation, oxidative stress, and stroke management. Their unique structures enable them to modulate receptor signaling and exhibit dual functions in cell protection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Casey A. Chamberlain, Marguerite Hatch, Timothy J. Garrett
Article
Endocrinology & Metabolism
Shawn A. Christensen, E'lysse A. Santana, Hans T. Alborn, Anna K. Block, Casey A. Chamberlain
Summary: This study investigated the molecular responses of maize to abiotic, biotic, and combinatorial stress using targeted and untargeted metabolomics. Results showed differential metabolite expression between heat stress, C. heterostrophus infection, and their controls, indicating that abiotic stress can predispose crops to more severe disease symptoms. The study highlights the importance of investigating defense chemistry in plants under combinatorial stress.
Article
Biochemistry & Molecular Biology
Monica F. Torrez Lamberti, Evon DeBose-Scarlett, Timothy Garret, Leslie Ann Parker, Josef Neu, Graciela L. Lorca
Article
Biochemistry & Molecular Biology
Casey A. Chamberlain, Marguerite Hatch, Timothy J. Garrett
Summary: This study introduces a novel application of PSI-MS for the analysis of bacterial EVs for the first time. The isolation and purification of EVs from bacterial culture supernatant using EV-specific affinity chromatography, characterization through nanoparticle tracking analysis, and identification of EV-derived metabolites, lipids, and peptides through PSI-MS represent a pioneering approach in the field of MS-based EV analysis.
Article
Biochemistry & Molecular Biology
Xinsong Du, Juan J. Aristizabal-Henao, Timothy J. Garrett, Mathias Brochhausen, William R. Hogan, Dominick J. Lemas
Summary: Clinical metabolomics is a novel approach for biomarker discovery, but ensuring reproducibility in clinical research using metabolomics data is challenging. Existing checklists may not be sufficient to guarantee reproducible metabolomics data processing. This paper introduces an eight-item checklist to guide researchers in making computational steps reproducible for clinical metabolomics studies.
Article
Cell Biology
Madelyn M. Jackstadt, Casey A. Chamberlain, Steven R. Doonan, Leah P. Shriver, Gary J. Patti
Summary: A multidimensional metabolomics platform was validated in adult zebrafish for evaluating the mode of action of a drug and its potential off-target effects.
DISEASE MODELS & MECHANISMS
(2022)
Article
Nutrition & Dietetics
Dominick J. Lemas, Xinsong Du, Bethany Dado-Senn, Ke Xu, Amanda Dobrowolski, Marina Magalhaes, Juan J. Aristizabal-Henao, Bridget E. Young, Magda Francois, Lindsay A. Thompson, Leslie A. Parker, Josef Neu, Jimena Laporta, Biswapriya B. Misra, Ismael Wane, Samih Samaan, Timothy J. Garrett
Summary: This study aims to identify unique metabolites that may impact infant health outcomes by analyzing the metabolome of human and bovine milk at 2 weeks of life. The results showed that more than 80% of the milk metabolome is shared between human and bovine milk during early lactation. These findings highlight untargeted metabolomics as a potential strategy to identify unique and shared metabolites in bovine and human milk that may relate to and impact infant health outcomes.
Article
Medical Laboratory Technology
Hoda Safari Yazd, Vanessa Y. Rubio, Casey A. Chamberlain, Richard A. Yost, Timothy J. Garrett
Summary: This study aimed to elucidate the human biochemistry in X chromosomal deletion disorders through metabolomic and lipidomic profiling, showing perturbations in metabolic pathways and lipids, impacting neurotransmitter generation and brain function. The findings suggest that rare X chromosomal deletion disorders are not only limited to alterations in local neuronal functions, but are also metabolic diseases.
JOURNAL OF MASS SPECTROMETRY AND ADVANCES IN THE CLINICAL LAB
(2021)