Article
Chemistry, Multidisciplinary
Jing-Lu Lv, Bing Wang, Xiao-Jing Liao, Shu-Wei Ren, Jun-Tao Cao, Yan-Ming Liu
Summary: A chemical-chemical redox cycling amplification strategy was introduced into a photocathodic immunosensing system to design a novel self-powered photochemical system for protein analysis.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Jianying Zhang, Nana Qin, Mingjing Wang, Xu Hun
Summary: A low-noise double-redox cycling signal amplification photoelectrochemical strategy based on Mo2C-graphyne-AuNPs modified electrode was reported for high sensitivity detection of A beta(1-40) oligomers. This technology demonstrated a promising sensing platform for detecting bioactive substances at ultralow levels.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biophysics
Jun-Tao Cao, Jing-Lu Lv, Xiao-Jing Liao, Shu-Hui Ma, Yan-Ming Liu
Summary: An ingenious visible light-induced membraneless self-powered PEC biosensing platform was developed in this study, which showed excellent performance with a wide linear range and low detection limit. The proposed system incorporated a redox cycling strategy for ultrasensitive split-type PEC bioanalysis, providing an effective approach for clinical diagnosis.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Xiao-Jing Liao, Hui-Jin Xiao, Jun-Tao Cao, Shu-Wei Ren, Yan-Ming Liu
Summary: The split-type PEC immunosensor developed in this study utilizes the cosensitized structure of Ag2S/ZnO nanocomposites as photoelectrode and the photogenerated hole-induced chemical redox cycling amplification strategy, demonstrating high sensitivity for cardiac troponin I (cTnI) detection. By integrating immunoreaction on 96-well plates with chemical redox cycling amplification on the photoelectrode, the immunosensor achieves a detection limit of 3.0 x 10(-15) g mL(-1) and a detection range of 1.0 x 10(-14) g mL(-1) to 1.0 x 10(-9) g mL(-1) for cTnI, showing potential for sensitive biosensing and bioanalysis.
Article
Chemistry, Analytical
Peng Qu, Cheng-Jie Li, Jin Hu, Ge Gao, Peng Lin, Wei-Wei Zhao
Summary: Organicphotoelectrochemical transistor (OPECT) biosensing is a promising approach for biomolecular sensing. This work demonstrates the direct enzymatic biocatalytic precipitation (BCP) modulation on a flower-like Bi(2)S(3) photosensitive gate, achieving high-efficiency OPECT operation with high transconductance (g (m)). The developed OPECT aptasensor shows good analysis performance for prostate-specific antigen (PSA).
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Yuecan Zhao, Junzhu Xiang, Hao Cheng, Xiaojuan Liu, Feng Li
Summary: The development of a novel flexible photoelectrochemical biosensor for miRNA determination showed promising potential for ultrasensitive disease diagnosis. The biosensor exhibited excellent analytical performance, selectivity, stability, flexibility, and practical applicability, making it a valuable tool for bioassays and early disease detection.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Tatjana Safarik, Aleksandar Karajic, Stephane Reculusa, Philip N. Bartlett, Nicolas Mano, Alexander Kuhn
Summary: This study reports the electrochemical elaboration and characterization of a 3D coaxial macroporous twin-electrode, which is obtained by a low-cost bottom-up approach using nanoengineered highly organized porous material. The resulting electrode architecture allows for signal amplification in electroanalytical measurements and has potential applications in various (bio)electroanalytical fields.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Yu Wang, Yuan Peng, Huanying Zhou, Zhixian Gao
Summary: The study reveals that the CRISPR-Cas14a system, similar to other members of the Cas family, not only provides high-sensitivity detection and gene editing ability but also has potential applications in DNA detection through fluorescence. This paper describes the development of a universal CRISPR-Cas14a-responsive ultrasensitive upconversion PEC sensor, which combines electrical and fluorescence signals for improved stability and sensitivity. The sensor has been successfully applied for the detection of small-molecule toxin T2 and protein kinase PTK7, showcasing its potential in ultrasensitive and cost-effective universal sensors for detection and diagnosis.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Kentaro Ito, Kumi Y. Inoue, Kosuke Ino, Hitoshi Shiku
Summary: In this study, a high-sensitivity dual immunoassay using Lumulus amebocyte lysate (LAL) and blood coagulation cascade reactions with redox cycling in a nanoscale-gap electrode was developed. The dual immunoassay showed high sensitivity for detecting two types of analytes, and the signal amplification by the cascade reactions and the use of redox cycling in a nanoscale gap contributed to the high sensitivity. The cross reaction between the LAL and coagulation cascade reactions was minimized by bisecting the beads after the immunocomplex formation. The dual immunoassay demonstrated its potential for clinical diagnosis requiring the detection of multiple analytes.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Stanislav Trashin, Francisco Morales-Yanez, Saranya Thiruvottriyur Shanmugam, Linda Paredis, Erik N. Carrion, Idalia Sariego, Serge Muyldermans, Katja Polman, Sergiu M. Gorun, Karolien De Wael
Summary: A new method for detecting the antigen marker of human toxocariasis is proposed, utilizing a rapid sandwich immunoassay sensor that achieves high sensitivity to pg/mL levels through a redox cycle involving photocatalytic oxidation and electrochemical reduction steps.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Feng Jiang, Shanghua Liu, Hui Dong, Xuelin Zhang, Shujun Wang, Yueyuan Li, Yueyun Li, Hongyou Cui, Qin Wei
Summary: In this study, a self-powered photoelectrochemical immunosensor was developed for sensitive detection of cardiac troponin. The sensor utilized a sulfur indium copper hollow nanocage as a photocathode and a hollow nanometer sphere heterojunction as a photoanode. By taking advantage of the coulomb force and internal electric field effect, the photoelectric response of the sensor was significantly improved. Additionally, the designed hollow materials with energy conversion function showed enhanced photon capture rate and more active sites for redox reactions, further enhancing the overall photoelectric response. This research provides insights into the design of nanomaterials with superior photoelectric activity and opens up possibilities for high sensitivity detection of other disease markers.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biophysics
Haiyang Wang, Xinyu Wan, Xin Wang, Meijin Li, Dianping Tang
Summary: In this study, a liposome-amplification photoelectrochemical (PEC) immunoassay was developed using ultrathin mesoporous bismuth chloride oxide nanosheets (BiOCl MSCN) for the highly selective and sensitive detection of carcinoembryonic antigen (CEA).
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Danli Hu, Haiyan Cui, Xinyang Wang, Fang Luo, Bin Qiu, Weicheng Cai, Hui Huang, Jian Wang, Zhenyu Lin
Summary: An innovative photo-electrochemical aptasensor based on Au/GaN photoelectrode has been developed for cancer biomarker detection. The sensor combines the characteristics of Au promoting charge transfer and aptamer high selectivity, showing high sensitivity and selectivity in detecting AFP concentration ranging from 1.0-150 ng/mL.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yonghuan Su, Lixia Su, Jiao Ran, Huafei Yi, Bingqian Liu
Summary: A photoelectrochemical (PEC) sensor was developed with a TiO2/CdIn2S4 co-sensitive structure and a g-C3N4-WO3 heterojunction as the photoanode to form a self-powered system. The photogenerated hole-induced biological redox cycle is used as a signal amplification strategy for Hg2+ detection. The presence of Hg2+ disrupts the biological cycle, leading to a decrease in photocurrent, enabling the detection of Hg2+.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Jun-Tao Cao, Jing-Lu Lv, Xiao-Jing Liao, Shu-Hui Ma, Yan-Ming Liu
Summary: This study validated the feasibility of the chemical-chemical redox cycling amplification strategy on a Z-scheme heterostructure photoelectrode, demonstrating its applicability in PEC immunoassay with a very low detection limit and wide linear range.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Tianyang Zhang, Siyuan Yu, Bing Wang, Yitong Xu, Xiaomei Shi, Weiwei Zhao, Dechen Jiang, Hongyuan Chen, Jingjuan Xu
Summary: The ideal single-cell viscometer has not been achieved, and existing methods have limitations. This study introduces a high spatiotemporal iontronic single-cell viscometer that can accurately monitor changes in cell viscosity.
Article
Chemistry, Multidisciplinary
Xian Zhang, Dan Luo, You-Wei Zheng, Xiao-Qiong Li, Juan Song, Wei-Wei Zhao, Hong-Yuan Chen, Jing-Juan Xu
Summary: The use of functional DNA nanostructures as carriers to transport proteins through solid-state nanopores has shown significant progress in single-protein-molecule detection. In this study, a 6.8 nm DNA tetrahedron was used as a carrier to successfully detect acetylcholinesterase with high specificity, low detection limit, and superior signal-to-noise ratio through an ultrasmall nanopipet. The practical applicability of this method was validated using serum samples from patients with Alzheimer's disease.
Article
Chemistry, Multidisciplinary
Xiao-Mei Shi, Yi-Tong Xu, Bing-Yu Zhou, Bing Wang, Si-Yuan Yu, Wei-Wei Zhao, Dechen Jiang, Hong-Yuan Chen, Jing-Juan Xu
Summary: Single-cell protein therapeutics has the potential to enhance our understanding of how a specific protein treats cells individually instead of averaging across populations. A double-barrel nanopipette was used in this study to address the challenge. The results showed that delivering protein DJ-1 into cells can upregulate antioxidant protein and protect neural cells from oxidative stress. This nanotool provides a perspective for future single-cell therapies involving different therapeutic modalities.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Nanoscience & Nanotechnology
Zhengjian Li, Mingzhi Wang, Yanyan Jia, Ruian Du, Tan Li, Yanping Zheng, Mingshu Chen, Yongcai Qiu, Keyou Yan, Wei-Wei Zhao, Pei Wang, Geoffrey I. N. Waterhouse, Sheng Dai, Yun Zhao, Guangxu Chen
Summary: Metal-oxide interfaces on Cu-based catalysts are crucial for the low-temperature water-gas shift reaction (LT-WGSR). However, developing catalysts with active and robust Cu-metal oxide interfaces under LT-WGSR conditions is challenging. In this study, a Cu@CeO2 catalyst with abundant CeO2/Cu2O/Cu interfaces was developed and showed significantly higher LT-WGSR activity compared to a pristine Cu catalyst. The Cu+/Cu0 interfaces were identified as the active sites, while the adjacent CeO2 nanoparticles played a key role in stabilizing the Cu+/Cu0 interfaces. This study contributes to the development of improved Cu-based catalysts for the LT-WGSR.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Qiqi Wu, Ruian Du, Peng Wang, Geoffrey I. N. Waterhouse, Jia Li, Yongcai Qiu, Keyou Yan, Yun Zhao, Wei-Wei Zhao, Hsin-Jung Tsai, Meng-Cheng Chen, Sung-Fu Hung, Xue Wang, Guangxu Chen
Summary: By thermally reducing Cu2O nanocubes under a CO atmosphere, a Cu2O-Cu nanocube hybrid catalyst (Cu2O(CO)) with a high density of Cu0/Cu+ interfaces, abundant nanograin boundaries with Cu(100) facets, and Cu[n(100)x(110)] step sites is successfully prepared. The Cu2O(CO) electrocatalyst exhibits a high C2+ Faradaic efficiency of 77.4% (56.6% for ethylene) during CO2 reduction under industrial current density of 500 mA/cm2, indicating the enhanced selectivity and productivity of C2+ products through surface and interface engineering.
Article
Chemistry, Multidisciplinary
Ge Gao, Jia-Hao Chen, Ming-Jian Jing, Jin Hu, Qin Xu, Cheng-Shuang Wang, Hong Zhou, Peng Lin, Guangxu Chen, Wei-Wei Zhao
Summary: This study addresses the challenge of achieving maximum transconductance (g(m)) at zero gate bias (V-G) in organic electrochemical transistors. By utilizing unique light-matter interplay in the newly emerged technique of organic photoelectrochemical transistor (OPECT), a dual-ligand photosensitive metal-organic framework (DL-PS-MOFs)/TiO2 nanorods (NRs) gated poly(ethylene dioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) OPECT is developed, demonstrating enhanced performance with maximum g(m) at zero V-G. The device is further biologically interfaced with a miRNA-triggered growth of DNA spheres for sensitive miRNA-21 detection.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Analytical
Feng-Zao Chen, Xiao-Xue Fu, Xiao-Jie Yu, Yu-Hang Qiu, Shi-Bin Ren, Yi-Chao Wang, De-Man Han, Wei-Wei Zhao
Summary: This study presents a biosensor based on MOF-on-MOF-derived semiconductor heterojunctions, which relies on the bioinduced AgI transition on ZnO@In2O3@AgI. The photocurrent switches from cathodic to anodic due to the generation of ZnO@In2O3@In2S3@Ag2S heterojunctions by bioproduced H2S. The biosensor shows good selectivity and sensitivity for detecting NSE with a low detection limit of 0.58 pg/mL.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jia-Hao Chen, Cheng-Shuang Wang, Zheng Li, Jin Hu, Si-Yuan Yu, Yi-Tong Xu, Peng Lin, Wei-Wei Zhao
Summary: In this study, a poly(1,4-diethynylbenzene) (pDEB)/NiO enhancement-mode organic photoelectrochemical transistor (OPECT) was developed for highly sensitive bioelectronic detection. The developed biosensor showed a linear range of 70 fg mL-1-10 ng mL-1 and a low detection limit of 28.5 fg mL-1.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Peng Qu, Cheng-Jie Li, Jin Hu, Ge Gao, Peng Lin, Wei-Wei Zhao
Summary: Organicphotoelectrochemical transistor (OPECT) biosensing is a promising approach for biomolecular sensing. This work demonstrates the direct enzymatic biocatalytic precipitation (BCP) modulation on a flower-like Bi(2)S(3) photosensitive gate, achieving high-efficiency OPECT operation with high transconductance (g (m)). The developed OPECT aptasensor shows good analysis performance for prostate-specific antigen (PSA).
ANALYTICAL CHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Mengmeng Gu, Hong Wang, Yuan-Cheng Zhu, Xiuming Wu, Ling Zhang, Yuming Dong, Wei-Wei Zhao, Guang-Li Wang
Summary: This study discloses a new mechanism of introducing surface oxygen vacancies (V(O)) in bismuth trioxide (Bi2O3) nanocrystals, which enables superior photostability and anti-interference capability in cathodic photoelectrochemical (PEC) bioanalysis. The surface V(O) can act as a separation center for charge carriers, facilitating the generation of cathodic photocurrent that is linearly correlated with the concentrations of targets, glucose-6-phosphate (G-6-P) and G6PD. This research not only provides a method to introduce surface V(O), but also enriches the toolbox of cathodic PEC bioassays and stimulates further interest in surface effect engineering in other fields.
ACS APPLIED NANO MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Yi-Li Liu, Si-Yuan Yu, Ruibing An, Yinxing Miao, Dechen Jiang, Deju Ye, Jing-Juan Xu, Wei-Wei Zhao
Summary: Biological channels can modulate ion transport behaviors rapidly and continuously in response to external stimuli, playing important roles in physiological and pathological processes in cells. Researchers have developed a bionic nanochannel by combining a cationic silicon-substituted rhodamine with a glass nanopipette to mimic biological channels for transmembrane single-cell quantification. The bionic nanochannel shows a fast and reversible response to glutathione (GSH), leading to a distinct and reversible switch in ionic current rectification. This tool enables in situ quantification of endogenous GSH in single cells and may be adapted for other endogenous biomolecules.
Article
Chemistry, Analytical
Xiaodi Wu, Zheng Li, Jin Hu, Shumin Wang, Yichao Wang, Peng Lin, Hong Zhou, Wei-Wei Zhao
Summary: Scientists have been seeking a solution for developing an organic photoelectrochemical transistor (OPECT) biosensor with a removed background, but this challenge remains. However, by tailoring a unique heterogeneous light antenna as the functional gating module and its cascade interaction with a proper channel, we have successfully addressed this issue. This achievement resulted in an OPECT with minimal background and the highest current gain of 19,000.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Yu-Yue Zhu, Tian-Tong Jiang, Ge Gao, Shi-Liang Wang, Xing-Wu Jiang, Yi-Tong Xu, Xiao-Fan Zhai, Hong Zhou, Wei-Wei Zhao
Summary: In this study, a DNA intercalation-enabled OPECT was developed for miRNA detection, which showed a high gain and low detection limit. The device generated anodic photocurrent through intercalation of [Ru(bpy)2dppz]2+ within the miRNA-initiated HCR-derived duplex DNA, resulting in target-dependent modulated channel current for quantitative miRNA-21 analysis. This study highlights the potential of DNA intercalation in organic electronics for innovative bioelectronics and biosensing.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Yu-Ting Huang, Ke-Xin Xu, Xing-Shi Liu, Zheng Li, Jin Hu, Ling Zhang, Yuan-Cheng Zhu, Wei-Wei Zhao, Hong-Yuan Chen, Jing-Juan Xu
Summary: This work combines chemical amplification with electronic amplification in the organic photoelectrochemical transistor (OPECT), achieving dual-amplified bioanalysis and sensitive detection of human immunoglobulin G. The study is significant for OPECT bioanalysis.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
You-Wei Zheng, Si-Yuan Yu, Zheng Li, Yi-Tong Xu, Wei-Wei Zhao, Dechen Jiang, Hong-Yuan Chen, Jing-Juan Xu
Summary: This work proposes and verifies the concept of single-cell miR therapy, and demonstrates high-precision miR-21 targeted therapy using a nanopipette. The experimental results show that the therapeutic effects can be achieved through direct intracellular drug administration and selective response of a PEC sensing interface. This work provides important insights into high-precision clinical miR therapy in personalized medicine.
