Article
Multidisciplinary Sciences
Shizhen Shen, Zhangjin Shen, Conghui Wang, Xiaodong Wu, Lingfang Wang, Lele Ye, Songfa Zhang, Xiaodong Cheng
Summary: In this study, a method for extracting extracellular vesicles (EVs) from epithelial ovarian cancer (EOC) tissues was optimized to investigate the effect of cryopreservation on the phenotypes, contents, and biological functions of the extracted EVs. The results showed that cryopreservation affected the yield and distribution of EVs, but not their morphology or particle mass ratio. The storage of tissues at -80°C increased both small and large EV yields, while the storage of lysates at -80°C decreased large EV yield and increased small EV yield. The cryopreserved EOC tissue retained a high percentage of proteins and miRNAs, and the extracted EVs could promote angiogenesis and invasive migration ability in human endothelial cells. This suggests that cryopreserved EOC tissue has clinical value for studying tissue-derived EVs, especially those related to EV proteins.
Review
Biochemistry & Molecular Biology
Tamara Camino, Nerea Lago-Baameiro, Maria Pardo
Summary: Extracellular vesicles (EVs) are nanosized lipid bilayer spheres that have emerged as new metabolic players in energy homeostasis and intercellular communication. They carry bioactive molecules and serve as a source of biomarkers and drug-delivery vehicles. The research on the role of EVs in metabolism, obesity, and associated pathologies is rapidly increasing. This review focuses on adipose tissue shed EVs, their characterization, size profile, and molecular cargo content, and also discusses the deleterious effects of EVs released by obese adipose tissue at both local and distant levels.
Review
Biochemistry & Molecular Biology
Ines Dinis Aires, Teresa Ribeiro-Rodrigues, Raquel Boia, Magda Ferreira-Rodrigues, Henrique Girao, Antonio Francisco Ambrosio, Ana Raquel Santiago
Summary: Microglial cells are important for maintaining neuronal homeostasis in the central nervous system, while chronic neuroinflammation plays a role in neurodegenerative diseases. Extracellular vesicles, such as apoptotic bodies, microvesicles, and exosomes, are involved in intercellular communication and may regulate microglial cell function. Understanding the role of microglial extracellular vesicles could provide insights into the mechanisms underlying neurodegeneration.
Review
Biochemistry & Molecular Biology
Katarzyna Horodecka, Markus Duchler
Summary: The establishment of CRISPR/Cas9 technology has revolutionized gene editing and therapeutic interventions, but challenges remain in effectively delivering components to living organisms. Utilizing extracellular vesicles as delivery devices may help overcome limitations and improve the clinical applications of this technology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Immunology
Chang Tian, Ke Wang, Min Zhao, Shan Cong, Xin Di, Ranwei Li
Summary: Sepsis, a life-threatening condition caused by a dysregulated host response to infection, is a major cause of mortality worldwide. Early diagnosis and effective treatment of sepsis remain challenging due to its rapid progression and heterogeneity among individuals. This review highlights the role of extracellular vesicles (EVs) in sepsis, including their impact on pathological processes, organ dysfunction, and potential applications such as early diagnosis, dynamic monitoring, precision therapies, and vaccine platforms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Marc Liebana-Jordan, Bruno Brotons, Juan Manuel Falcon-Perez, Esperanza Gonzalez
Summary: Extracellular vesicles play a crucial role in intercellular communication, especially in fungi, by maintaining cell structure, defending against the environment, and potentially impacting host interactions and disease outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Chemistry, Multidisciplinary
Fei Teng, Martin Fussenegger
Summary: Extracellular vesicles (EVs) are nano-sized secreted vesicles containing various biomolecules, which have low immunogenicity and can functionally modify recipient cells by transferring bioactive constituents. Therefore, they are considered as an excellent candidate for the next generation drug delivery system.
Review
Chemistry, Multidisciplinary
Yujia Hao, Haoyue Song, Zilan Zhou, Xiaohang Chen, Huifei Li, Yuan Zhang, Jie Wang, Xiuyun Ren, Xing Wang
Summary: Extracellular vesicles (EVs) are important vehicles for intercellular communication, with different physiological and pathological effects, and can be used as biological therapeutic agents. In some cases, the release of EVs can exacerbate disease progression, while inhibition may delay disease development.
JOURNAL OF CONTROLLED RELEASE
(2021)
Review
Cell Biology
Sarah Beck, Bernhard Hochreiter, Johannes A. Schmid
Summary: Extracellular vesicles (EVs) play crucial roles in intercellular communication by transferring nucleotides and proteins, and are associated with diseases such as cancer, inflammation, and thrombosis. They have the potential to serve as biomarkers for diagnosis and as vehicles for therapeutics in the future.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Amit Chatterjee, Ruchira Singh
Summary: Extracellular vesicles (EVs) are secreted membrane vesicles that come in various sizes, including exosomes (-30-200 nm) and microvesicles (MVs) that are around 100-1,000 nm in size. These EVs have been found to play a crucial role in autocrine, paracrine, and endocrine signaling, and are associated with a range of human disorders, including retinal degenerative diseases such as age related macular degeneration (AMD) and diabetic retinopathy (DR). In this review, the authors provide an overview of the current understanding of the role of EVs in retinal physiology and specifically focus on how disease-related changes in EVs can impact specific retinal diseases. Additionally, they discuss the potential of EVs in diagnostic and therapeutic strategies for targeting retinal diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Bryan John Abel Magoling, Anthony Yan-Tang Wu, Yen-Ju Chen, Wendy Wan-Ting Wong, Steven Ting-Yu Chuo, Hsi-Chien Huang, Yun-Chieh Sung, Hsin Tzu Hsieh, Poya Huang, Kang-Zhang Lee, Kuan-Wei Huang, Ruey-Hwa Chen, Yunching Chen, Charles Pin-Kuang Lai
Summary: Extracellular vesicles (EVs), including small EVs (sEVs, exosomes) and medium to large EVs (bEVs, microvesicles), play important roles in intercellular communication in breast cancer. This study identifies distinct properties and functions of bEVs and sEVs in breast cancer, showing that bEVs are released in a higher ratio in aggressive triple-negative breast cancer subtype. Both bEVs and sEVs promote tumor growth in a murine model, and they share common tumor-progression-associated EV surface membrane proteins. Depletion of these proteins reduces the protumorigenic potential of EVs. This study highlights the significance of bEVs in breast cancer and suggests their potential diagnostic and therapeutic applications.
ADVANCED MATERIALS
(2023)
Review
Cell Biology
Zhenzhen Mo, Jia Yang Alex Cheong, Lirong Xiang, Minh T. N. Le, Andrew Grimson, Daniel Xin Zhang
Summary: Metastasis, the dissemination of primary tumor cells, is a major cause of cancer-related deaths. Metastatic organotropism, the specific distribution pattern of distant metastasis, is influenced by various factors. Extracellular vesicles (EVs) play a crucial role in organ-specific metastasis, carrying bioactive cargo that can modulate the pre-metastatic niche and influencing diagnostic and prognostic values. Counteracting EV functions could be a novel therapeutic strategy to target metastasis. More research is needed to understand the mechanisms of EVs in organ-specific metastasis for clinical translation.
CELL PROLIFERATION
(2021)
Review
Biochemical Research Methods
William Phillips, Eduard Willms, Andrew F. Hill
Summary: Extracellular vesicles (EVs) are membrane-enclosed nanoparticles released by cells, playing a role in intercellular communication and various physiological and pathological processes. Current size-based isolation methods are not optimal, and proteomic analysis may offer alternative approaches for EV purification.
Review
Biochemistry & Molecular Biology
Hiu Yee Kwan, Minting Chen, Keyang Xu, Baisen Chen
Summary: This article critically reviews the impact of obesity on the exosomal cargo contents and the biogenesis of adipocyte-derived exosomes. It also discusses the differences in exosomal contents in different adipose depots, as well as the impact of obesity on exosomes derived from stromal vascular fraction components. The review also touches upon the development and potential application of exosome-based therapy for obesity and its comorbidities.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Oncology
Akbar Lulu Marzan, Sarah Elizabeth Stewart
Summary: Pancreatic cancer is a highly lethal cancer with a 5-year survival rate of less than 10%, largely due to factors such as inadequate diagnostics, rapid metastasis, and chemoresistance. Research on extracellular vesicles in pancreatic cancer progression and chemoresistance is crucial for identifying new treatment options. Accumulating evidence suggests that extracellular vesicles may serve as biomarkers for early detection and as potential therapeutic targets or drug delivery tools for the treatment of pancreatic cancer.
Article
Gastroenterology & Hepatology
Ferenc Emil Mozes, Jenny A. Lee, Emmanuel Anandraj Selvaraj, Arjun Narayan Ajmer Jayaswal, Michael Trauner, Jerome Boursier, Celine Fournier, Katharina Staufer, Rudolf E. Stauber, Elisabetta Bugianesi, Ramy Younes, Silvia Gaia, Monica Lupsor-Platon, Salvatore Petta, Toshihide Shima, Takeshi Okanoue, Sanjiv Mahadeva, Wah-Kheong Chan, Peter J. Eddowes, Gideon M. Hirschfield, Philip Noel Newsome, Vincent Wai-Sun Wong, Victor de Ledinghen, Jiangao Fan, Feng Shen, Jeremy F. Cobbold, Yoshio Sumida, Akira Okajima, Joern M. Schattenberg, Christian Labenz, Won Kim, Myoung Seok Lee, Johannes Wiegand, Thomas Karlas, Yusuf Yilmaz, Guruprasad Padur Aithal, Naaventhan Palaniyappan, Christophe Cassinotto, Sandeep Aggarwal, Harshit Garg, Geraldine J. Ooi, Atsushi Nakajima, Masato Yoneda, Marianne Ziol, Nathalie Barget, Andreas Geier, Theresa Tuthill, M. Julia Brosnan, Quentin Mark Anstee, Stefan Neubauer, Stephen A. Harrison, Patrick M. Bossuyt, Michael Pavlides
Summary: Liver biopsy is still necessary for fibrosis staging in patients with non-alcoholic fatty liver disease. This study evaluated the diagnostic performance of liver stiffness measurement, FIB-4 Index, and NAFLD Fibrosis Score, and showed that sequential combinations of markers can reduce the need for liver biopsies.
Article
Gastroenterology & Hepatology
Grazia Pennisi, Marco Enea, Manuel Romero-Gomez, Mauro Vigano, Elisabetta Bugianesi, Vincent W. -s Wong, Anna Ludovica Fracanzani, Giada Sebastiani, Jerome Boursier, Annalisa Berzigotti, Mohammed Eslam, Javier Ampuero, Amine Benmassaoud, Claudia La Mantia, Yuly P. Mendoza, Jacob George, Antonio Craxi, Calogero Camma', Victor de Ledinghen, Salvatore Petta
Summary: The occurrence of extrahepatic events (EHE) in patients with non-alcoholic fatty liver disease (NAFLD) is relevant and increases according to the severity of liver fibrosis, while the risk of liver-related events (LRE) is low in patients with mild fibrosis.
ALIMENTARY PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Gastroenterology & Hepatology
Jerome Boursier, Hannes Hagstrom, Mattias Ekstedt, Clemence Moreau, Martin Bonacci, Sandrine Cure, Javier Ampuero, Patrik Nasr, Lilian Tallab, Clemence M. Canivet, Stergios Kechagias, Yolanda Sanchez, Eloise Dincuff, Ana Lucena, Marine Roux, Jeremie Riou, Aldo Trylesinski, Manuel Romero-Gomez
Summary: FIB4 and VCTE demonstrate good accuracy in predicting liver-related events in NAFLD patients, with VCTE showing similar prognostic accuracy as liver biopsy. FIB4 and VCTE are independent predictors of liver-related events in the entire study cohort.
JOURNAL OF HEPATOLOGY
(2022)
Review
Nutrition & Dietetics
Laurent-Emmanuel Monfoulet, Maria Carmen Martinez
Summary: Extracellular vesicles, including nanovesicles derived from the endosome system, are gaining interest due to their potential role in cell-to-cell communication and biomarker use. Studies suggest that poor-quality diets may increase levels of circulating large EVs, but this can be countered by healthy foods and micronutrients. However, more research is needed to understand the content and biological functions of diet-induced EVs.
Review
Nutrition & Dietetics
Sayani Mukherjee, Silje Skrede, Edward Milbank, Ramaroson Andriantsitohaina, Miguel Lopez, Johan Ferno
Summary: Antipsychotic drugs play a crucial role in the treatment of schizophrenia and other psychoses. However, many of these drugs have side effects such as increased appetite, weight gain, and increased risk for diabetes and cardiovascular disease. Understanding the molecular mechanisms behind drug-induced hyperphagia is important for the development of new drugs and advancing our knowledge of appetite regulation.
FRONTIERS IN NUTRITION
(2022)
Article
Obstetrics & Gynecology
Pascal Froment, Ingrid Plotton, Cecilia Giulivi, Stephane Fabre, Rita Khoueiry, Nizar Mourad, Sandrine Horman, Christelle Rame, Charlene Rouillon, Jeremy Grandhaye, Yves Bigot, Claire Chevaleyre, Remy Le Guevel, Patricia Mallegol, Ramaroson Andriantsitohaina, Fabrice Guerif, Jerome Tamburini, Benoit Viollet, Marc Foretz, Joelle Dupont
Summary: The lack of alpha 1AMPK in granulosa cells impacts various biological processes and leads to a hyperandrogenic response.
HUMAN REPRODUCTION
(2022)
Article
Pharmacology & Pharmacy
Abir Salek, Mouna Selmi, Mahassen Barboura, M. Carmen Martinez, Leila Chekir-Ghedira, Ramaroson Andriantsitohaina
Summary: This study utilized small extracellular vesicles (sEVs) to enhance the stability and efficacy of berberine hydrochloride (BRB). The results showed that sEVs loaded with BRB had greater inhibitory effects on proliferation, migration, and capillary-like formation in tumor cells and endothelial cells compared to free BRB. Therefore, sEV-loaded BRB may be considered as an innovative delivery method to prevent tumorigenesis and angiogenesis.
Article
Endocrinology & Metabolism
Edward Milbank, Nathalia Dragano, Xavi Vidal-Gomez, Veronica Rivas-Limeres, Pablo Garrido-Gil, Mireille Wertheimer, Sylvain Recoquillon, Maria P. Pata, Jose Luis Labandeira-Garcia, Carlos Dieguez, Ruben Nogueiras, M. Carmen Martinez, Ramaroson Andriantsitohaina, Miguel Lopez
Summary: The study found that using a specific type of extracellular vesicles loaded with a defective AMPK alpha 1 gene can alleviate symptoms such as obesity and metabolic disorders in mice.
METABOLISM-CLINICAL AND EXPERIMENTAL
(2023)
Article
Cell Biology
Lionel Fizanne, Alexandre Villard, Nadia Benabbou, Sylvain Recoquillon, Raffaella Soleti, Erwan Delage, Mireille Wertheimer, Xavier Vidal-Gomez, Thibauld Oullier, Samuel Chaffron, M. Carmen Martinez, Michel Neunlist, Jerome Boursier, Ramaroson Andriantsitohaina
Summary: The study investigates the role of fEVs and cEVs in liver diseases such as NAFLD and NASH. NASH-fEVs were found to have deleterious effects, increasing intestinal permeability and reducing expression of tight junction proteins through the involvement of nmMLCK and lipopolysaccharide pathways.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Gastroenterology & Hepatology
Jenny Lee, Max Westphal, Yasaman Vali, Jerome Boursier, Salvatorre Petta, Rachel Ostroff, Leigh Alexander, Yu Chen, Celine Fournier, Andreas Geier, Sven Francque, Kristy Wonders, Dina Tiniakos, Pierre Bedossa, Mike Allison, Georgios Papatheodoridis, Helena Cortez-Pinto, Raluca Pais, Jean-Francois Dufour, Diana Julie Leeming, Stephen Harrison, Jeremy Cobbold, Adriaan G. Holleboom, Hannele Yki-Jarvinen, Javier Crespo, Mattias Ekstedt, Guruprasad P. Aithal, Elisabetta Bugianesi, Manuel Romero-Gomez, Richard Torstenson, Morten Karsdal, Carla Yunis, Joern M. Schattenberg, Detlef Schuppan, Vlad Ratziu, Clifford Brass, Kevin Duffin, Koos Zwinderman, Michael Pavlides, Quentin M. Anstee, Patrick M. Bossuyt, LITMUS Investigators
Summary: Detection of NASH and at-risk NASH can be improved by constructing independent machine learning models for each component, using only clinical predictors. Adding biomarkers only improved the accuracy of fibrosis.
Article
Multidisciplinary Sciences
Jerome Boursier, Marine Roux, Charlotte Costentin, Julien Chaigneau, Celine Fournier-Poizat, Aldo Trylesinski, Clemence M. Canivet, Sophie Michalak, Brigitte Le Bail, Valerie Paradis, Pierre Bedossa, Natalie Sturm, Victor de Ledinghen, Philip. N. Newsome
Summary: The practical rules for the early non-invasive diagnosis of cirrhosis in NAFLD are not well defined. Here, the authors develop and validate two diagnostic tools: a stepwise stratification algorithm including a cirrhosis group, and a risk prediction chart providing a personalized assessment of the individual probability of cirrhosis.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Matthieu Ferragu, Luisa Vergori, Vincent Le Corre, Sarah Bellal, Maria del Carmen Martinez, Pierre Bigot
Summary: lEVs derived from kidney cancer patients may promote tumor growth and cancer progression, as demonstrated in a mouse model.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Endocrinology & Metabolism
Edward Milbank, Nathalia R. V. Dragano, Ismael Gonzalez-Garcia, Marcos Rios Garcia, Veronica Rivas-Limeres, Liliana Perdomo, Gregory Hilairet, Francisco Ruiz-Pino, Patricia Mallegol, Donald A. Morgan, Ramon Iglesias-Rey, Cristina Contreras, Luisa Vergori, Juan Cunarro, Begona Porteiro, Aleix Gavalda-Navarro, Rebecca Oelkrug, Anxo Vidal, Juan Roa, Tomas Sobrino, Francesc Villarroya, Carlos Dieguez, Ruben Nogueiras, Cristina Garcia-Caceres, Manuel Tena-Sempere, Jens Mittag, M. Carmen Martinez, Kamal Rahmouni, Ramaroson Andriantsitohaina, Miguel Lopez
Summary: Milbank et al. demonstrate that targeted delivery of AMPK alpha 1 in SF1 neurons of the VMH using small extracellular vesicles can lead to weight loss through increased brown adipose tissue thermogenesis, independent of feeding.
Article
Endocrinology & Metabolism
Liujun Chen, Lisha Li, Donghong Cui, Yiheng Huang, Haibin Tong, Haleh Zabihi, Shuxia Wang, Yadan Qi, Ted Lakowski, Lin Leng, Suixin Liu, Hong Wu, Lawrence H. Young, Richard Bucala, Dake Qi
Summary: Attenuation of adipose hormone sensitive lipase (HSL) may impair lipolysis and exacerbate obesity. Cytokine macrophage migration inhibitory factor (MIF) plays a role in regulating adipose HSL and adipocyte hypertrophy. Both intracellular and extracellular MIF have opposing effects on HSL, but extracellular action predominates to downregulate HSL and exacerbate obesity development during high-fat diet (HFD).
MOLECULAR METABOLISM
(2024)
Article
Endocrinology & Metabolism
Mengyang Tang, Yi Zhang, Rong Zhang, Yuemei Zhang, Jiangfei Zheng, Daixi Wang, Xinyu Wang, Jing Yan, Cheng Hu
Summary: This study aimed to explore the role of GPSM1 in POMC neurons and the underlying mechanisms in metabolic homeostasis. Through various molecular, biochemical, immunofluorescent, immunohistochemical analyses, and cell culture studies, the study revealed the pathophysiological role of GPSM1 in POMC neurons and its regulation of POMC neuron activity.
MOLECULAR METABOLISM
(2024)
