Article
Biochemistry & Molecular Biology
Anirban Dasgupta, Payel Mondal, Sambit Dalui, Chandrima Das, Siddhartha Roy
Summary: The plant homeodomain (PHD) finger of UBR7 is essential for its association with E2 UbcH6 and consequent ubiquitin transfer to histone H2B. The mechanism of ubiquitin transfer by UBR7 differs markedly from that of RNF20.
Article
Biochemistry & Molecular Biology
Zhiheng Deng, Huasong Ai, Maoshen Sun, Zebin Tong, Yunxiang Du, Qian Qu, Liying Zhang, Ziyu Xu, Shixian Tao, Qiang Shi, Jia-Bin Li, Man Pan, Lei Liu
Summary: This study elucidates the mechanism of H2B monoubiquitylation and highlights the critical role of nucleosomal DNA in mediating E3 ligase recognition.
Article
Pharmacology & Pharmacy
Shu-Ze Zheng, Xiao-Wen Zhang, Xiao-Min Song, Zhuo Yang, Lu Yao, Peng-Fei Tu, Ke-Wu Zeng
Summary: This study discovered a natural small-molecule compound ECL that inhibits microglia-mediated neuroinflammation by editing the monoubiquitination of histone H2B. ECL modifies the previously undisclosed K46 site in H2B and recruits the E3 ubiquitin ligase to promote H2Bub1 at K120, leading to disruption of AP-1 binding to proinflammatory genes in microglia.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Nathan J. VanDusen, Julianna Y. Lee, Weiliang Gu, Catalina E. Butler, Isha Sethi, Yanjiang Zheng, Justin S. King, Pingzhu Zhou, Shengbao Suo, Yuxuan Guo, Qing Ma, Guo-Cheng Yuan, William William Pu
Summary: The study utilized in vivo somatic Cas9 mutagenesis in mice to conduct a forward genetic screen for regulators of cardiomyocyte maturation, identifying transcriptional regulators such as RNF20 and RNF40. Mechanistic studies showed that these regulators control dynamic gene expression changes essential for cardiomyocyte maturation.
NATURE COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Junjiang Fu, Li Liao, Kyathegowdanadoddi Srinivasa Balaji, Chunli Wei, Jaehoon Kim, Jiangzhou Peng
Summary: RNF40 is an interesting new gene that plays a crucial role in cancer development, progression, and metastasis through its protein ubiquitylation activity, highlighting its important epigenetic function.
Editorial Material
Biochemistry & Molecular Biology
Joanna R. Morris
Summary: New findings show that BARD1, a partner of BRCA1, plays a crucial role in positioning the E2 ubiquitin-conjugating enzyme and promoting DNA damage-induced chromatin recruitment, enhancing the function of BRCA1.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2021)
Editorial Material
Plant Sciences
Yusheng Zhao, Zhiyong Liu
Summary: Zhang et al. (2023) demonstrated through association analysis that the wheat E3 ubiquitin ligase TaAIRP2-1B positively regulates spike length by involving an upstream regulator, TaERF3, and a downstream target, TaHIPP3. This study provides a deeper understanding of spike architecture regulation and offers an alternative molecular module for designing wheat with increased yield potential.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Review
Virology
Chunfu Zheng, Yan-Dong Tang
Summary: MARCH proteins play critical roles in viral infections by directly antagonizing different steps of the viral life cycle and promoting viral infection.
Article
Biochemistry & Molecular Biology
Shouping Wang, Kun Cao, Yuting Liao, Wei Zhang, Jihua Zheng, Xiaocui Li, Mengting Huang, Yonghao Zhong, Xiao Hu, De Chen, Yanjie Wang
Summary: CDCA2 is identified as a prognostic biomarker for HCC patients, with its amplification closely associated with HCC patient recurrence and survival. CDCA2 promotes HCC cell growth and inhibits apoptosis, while protecting HCC cells against oxidative damage through the BRCA1-NRF2 cascade.
Article
Agronomy
Daijing Zhang, Xiaoxu Zhang, Wu Xu, Tingting Hu, Jianhui Ma, Youfu Zhang, Jian Hou, Chenyang Hao, Xueyong Zhang, Tian Li
Summary: This study identified TaGW2L as a novel positive regulator of heading date in wheat. TaGW2L exhibited similar functions to TaGW2 and its overexpression led to an earlier heading date in wheat. This study sheds light on the role of TaGW2L in wheat heading date and provides potential applications for crop improvement.
Article
Biochemistry & Molecular Biology
Ya-Yun Chen, Xiang-Hong Ran, Run-Ze Ni, Dan Mu
Summary: TRIM28 is identified as a negative regulator of the RLR signaling pathway by targeting MAVS for proteasome-mediated degradation, which inhibits the production of type I interferons and proinflammatory cytokines induced by MAVS.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Plant Sciences
Jae Ho Kim, Irfan Ullah Khan, Moon Seok Kim, Yong Weon Seo
Summary: The wheat histone H2B monoubiquitination enzyme TaHUB2 interacts with H2B and exhibits ubiquitination activities, leading to decreased levels of H2Bub1 after vernalization. TaHUB2 transcription levels decrease with increased vernalization periods, and the protein is rapidly degraded in cell-free extracts within 3-6 hours after vernalization. This research provides new insights into the molecular characterization of wheat RING-type E3 ligase and its potential roles.
JOURNAL OF PLANT INTERACTIONS
(2021)
Review
Plant Sciences
Guoliang Han, Ziqi Qiao, Yuxia Li, Zongran Yang, Chengfeng Wang, Yuanyuan Zhang, Lili Liu, Baoshan Wang
Summary: RING zinc finger proteins play important roles in plant growth, development, and the response to abiotic stresses. They function as E3 ubiquitin ligases by modifying and degrading stress-related proteins in the cytoplasm and nucleus. Under abiotic stress, they may act as sensors or ABA receptors on the plasma membrane, or as transcription factors in the nucleus to regulate downstream gene expression.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Adrian B. Mehrtash, Mark Hochstrasser
Summary: Endoplasmic reticulum-associated degradation (ERAD) is a protein quality control pathway that removes misfolded proteins from the ER. Overexpression of ERAD factors inhibits the degradation of specific substrates. This study reveals a potential retrotranslocation mechanism independent of ERAD E3 complexes.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Gabriel Fenteany, Gaurav Sharma, Paras Gaur, Attila Borics, Edit Weber, Erno Kiss, Lajos Haracska
Summary: This study discovered a series of xanthenes that inhibit PCNA ubiquitination and disrupt the Rad6-ubiquitin thioester formation and Rad6-Rad18 interaction. These compounds can serve as first-in-class probes for studying Rad6 function and the association of Rad6 and Rad18, and potentially be therapeutic agents in cancer progression inhibition.
Article
Medicine, Research & Experimental
Nahal Haddadi, Glena Travis, Najah T. Nassif, Ann M. Simpson, Deborah J. Marsh
COLD SPRING HARBOR PERSPECTIVES IN MEDICINE
(2020)
Article
Biochemistry & Molecular Biology
Alexander J. Cole, Kristie-Ann Dickson, Christopher Liddle, Clare Stirzaker, Jaynish S. Shah, Roderick Clifton-Bligh, Deborah J. Marsh
Summary: The cancer-associated chromatin landscape is altered in response to therapeutic DNA damage, influencing gene expression and cell fate. Following treatment, there is a reduction in global levels of H2Bub1 and an increase in p53 levels. However, H2Bub1 is enriched downstream of specific genes, including key cancer-related genes, regulating gene expression and cellular response to therapeutic DNA damage.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Oncology
Deborah J. Marsh, Yue Ma, Kristie-Ann Dickson
Article
Biochemistry & Molecular Biology
Kristie-Ann Dickson, Tao Xie, Christian Evenhuis, Yue Ma, Deborah J. Marsh
Summary: PARP inhibitors have shown efficacy in treating tumors with BRCA gene defects, improving survival outcomes. Variability exists between different PARP inhibitors in terms of chemical structure, toxicity, and cell survival, with acquired resistance being a concerning issue that needs further exploration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Christine Bolitho, Michelle Moscova, Robert C. Baxter, Deborah J. Marsh
Summary: AREG plays a significant role in EOC, promoting migration and proliferation of EOC cells through activation of PI3-K signaling, independent of MAPK signaling. Both autocrine loop of AREG and the activity of endogenous AREG impact EOC cell migration and proliferation.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Christine Yee, Kristie-Ann Dickson, Mohammed N. Muntasir, Yue Ma, Deborah J. Marsh
Summary: Ovarian cancer has the highest mortality rate among gynecological malignancies. Different histotypes of the cancer respond differently to treatments, with chemoresistance being a key factor in incurable disease. While PARP inhibitors and bevacizumab are currently used in clinical settings, further research is needed to predict patient responses to these drugs effectively.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Dongli Liu, Gunnar F. Kaufmann, James B. Breitmeyer, Kristie-Ann Dickson, Deborah J. Marsh, Caroline E. Ford
Summary: This study investigated the potential of Zilovertamab, a ROR1-targeting therapy, in treating high-grade serous ovarian cancer and endometrial cancer. The results showed that Zilovertamab alone could inhibit tumor cell proliferation, but its combination with commonly used chemotherapy drugs did not enhance the anti-proliferative activity.
Review
Oncology
Tao Xie, Kristie-Ann Dickson, Christine Yee, Yue Ma, Caroline E. Ford, Nikola A. Bowden, Deborah J. Marsh
Summary: Synthetic lethality approaches involving the combination of events have shown significant advances in the treatment of women with ovarian cancer who have defects in the HRR pathway. PARP inhibitors have been approved for the clinical management of ovarian cancer, but challenges remain in overcoming acquired resistance, extending benefits to more patients, combining with other drugs, and developing new therapies.
Review
Oncology
Glena Travis, Eileen M. Mcgowan, Ann M. Simpson, Deborah J. Marsh, Najah T. Nassif
Summary: PTENP1, the processed pseudogene of PTEN, acts as a competitive endogenous RNA to alter the abundance of PTEN by competing with specific miRNAs for binding. Disruption of the PTENP1-miRNA-PTEN axis may lead to cancer but also provide novel therapeutic opportunities.
Article
Materials Science, Biomaterials
Nidhi Joshi, Dongli Liu, Kristie-Ann Dickson, Deborah J. Marsh, Caroline E. Ford, Martina H. Stenzel
Summary: This study investigated the delivery of ROR2 siRNA into HGSOC cells using nanoparticles and found that ROR2 siRNA conjugated PICs nanoparticles significantly inhibited migration and invasion ability. This suggests that ROR2 targeting therapy using biocompatible nanoparticles shows promise in treating HGSOC, including platinum resistant forms.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)