Article
Chemistry, Analytical
Pengfei Zhang, Rui Wang, Zijian Wan, Xinyu Zhou, Guangzhong Ma, Jayeeta Kolay, Jiapei Jiang, Shaopeng Wang
Summary: The article introduces a total internal reflection-based evanescent scattering microscopy technique that can detect single proteins without labeling and provides a high-precision measurement method for protein binding kinetics.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
T. Schoenfeldova, H. Okur, V. Vezocnik, I. Iacovache, C. Cao, M. Dal Peraro, P. Macek, B. Zuber, S. Roke
Summary: This study demonstrates an ultrasensitive method for direct detection of protein binding to liposome membranes using high throughput second harmonic scattering (SHS). The approach allows label-free and noninvasive probing of protein-membrane interactions at sub-picomolar concentrations in 3D systems.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Biochemical Research Methods
Xiaoyong Chen, Pin Xu, Wenwei Lin, Jin Jiang, Hang Qu, Xuehao Hu, Jinghua Sun, Yukun Cui
Summary: This study proposes a plasmonic titled fiber Bragg grating biosensor for the detection of breast cancer cells. Experimental results demonstrate that the biosensor has good detection performance, capable of detecting low concentrations of breast cancer cells in a short period of time. The sensor has a simple structure, easy manufacturing process, making it a promising choice for future cancer cell detection.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Chemistry, Analytical
Thakshila Liyanage, Meimei Lai, Gymama Slaughter
Summary: We have developed a novel label-free ultrasensitive tapered optical fiber plasmonic biosensor that can successfully detect a panel of microRNAs with good selectivity. The biosensor showed high refractive index sensitivity and achieved a limit of detection between 103 aM and 261 aM for the microRNA panel. It also demonstrated successful detection of clinically relevant concentrations of microRNAs in human serum with a LOD between 1.097 fM and 1.220 fM. This approach could potentially broaden the applications of biosensors for detecting and quantifying large arrays of microRNAs.
ANALYTICA CHIMICA ACTA
(2021)
Review
Chemistry, Multidisciplinary
Andrei V. Kabashin, Vasyl G. Kravets, Alexander N. Grigorenko
Summary: Label-free optical biosensing has great potential in biomedical diagnostics, environmental and food safety, and security. However, it lacks the sensitivity of traditional labelling methods. This article explores ways to overcome the sensitivity barriers and achieve superior sensitivity in label-free biosensing through novel structural architectures, artificial materials, and the use of optical phase as a sensing parameter.
CHEMICAL SOCIETY REVIEWS
(2023)
Article
Polymer Science
Yi-Jung Lu, Han-Yun Hsieh, Wen-Chang Kuo, Pei-Kuen Wei, Horn-Jiunn Sheen, Hidetoshi Tahara, Te-Wei Chiu, Yu-Jui Fan
Summary: The study proposed a multiplex detection system by integrating LSPR sensing array and microfluidic channels, proving successful in detecting different targets in parallel channels.
Article
Chemistry, Multidisciplinary
Yi-Nan Liu, Zhen-Ting Lv, Wen-Li Lv, Dong-Feng Liu, Xian-Wei Liu
Summary: We developed a label-free and noninvasive imaging method to measure electron transfer in microbial cells. By imaging the redox reaction of cytochromes in microbial cells, we obtained the electrochemical activity parameters of individual cells, revealing heterogeneities in electron transfer at the single-cell level.
Article
Nanoscience & Nanotechnology
Yuye Wang, Yurui Hu, Ruibin Xie, Qi Zeng, Yanhang Hong, Xi Chen, Pengcheng Zhang, Lin Zeng, Yi Zhang, Shuwen Zeng, Hui Yang
Summary: miRNAs are important biomarkers for the development and progression of various cancers. Detecting miRNAs is crucial for early-stage diagnostics and prognostics of multiple diseases. This study proposes a MXene-enhanced plasmonic biosensor for real-time and label-free detection of miRNA, with ultrasensitive detection limit and the ability to distinguish single-base mismatched miRNA. The biosensor can detect miRNA in complex media without the need for extra labels or amplification tags, holding great potential in biological applications and clinical diagnostics.
Article
Chemistry, Analytical
Li Luo, Jiewei Yang, Zhi Li, Hua Xu, Lei Guo, Lili Wang, Yuxia Wang, Longlong Luo, Jing Wang, Pingping Zhang, Ruifu Yang, Weijun Kang, Jianwei Xie
Summary: A novel label-free immunosensor method was developed in this study for differentiation and quantification of ricin and abrin, showing rapid and accurate measurement in various complex matrices. The results demonstrate the potential practical application value of this method.
Review
Chemistry, Analytical
Pengcheng Wang, Hao Sun, Wei Yang, Yimin Fang
Summary: This paper reviews the application of label-free optical methods for the rapid detection of pathogenic bacteria. These methods have advantages of rapidity, simplicity, and relatively low-cost, and can overcome the limitations of traditional detection techniques, promoting precise therapy initiation.
Article
Materials Science, Multidisciplinary
Victoria O. Shipunova, Maxim P. Nikitin, Mariia M. Belova, Sergey M. Deyev
Summary: The study shows that multiparametric surface plasmon resonance is an effective label-free method for investigating the dynamic binding process between nanoparticles and cells, where both the angle and intensity of the peak in the full angular spectrum can be utilized.
MATERIALS TODAY COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Lucia Sarcina, Giuseppe Felice Mangiatordi, Fabrizio Torricelli, Paolo Bollella, Zahra Gounani, Ronald Osterbacka, Eleonora Macchia, Luisa Torsi
Summary: The study developed a reliable and label-free detection method for HIV-1 p24 protein using multi-parameter SPR. Different antibody immobilization techniques on the surface led to varying coverage and binding signals. The chemically modified system showed improved sensitivity and selectivity in detecting HIV-1 p24.
Article
Food Science & Technology
Stefan Jaric, Aabha Bajaj, Vladimir Vukic, Ivana Gadjanski, Ibrahim Abdulhalim, Ivan Bobrinetskiy
Summary: In this study, a new method for label-free detection of cyanotoxin molecules using a graphene-modified surface plasmon resonance (SPR) aptasensor is reported. Molecular dynamic simulation reveals the strongest binding sites between the aptamer and cylindrospermopsin (CYN) are located between C18-C26 pairs. The SPR sensor was modified using the wet transfer method of CVD monolayer graphene. For the first time, graphene functionalized with an aptamer was used as a bioreceptor in conjunction with SPR for the detection of CYN, showing noticeable changes in the optical signal in response to concentrations below the maximum tolerable level of 1 μg/L and high specificity.
Article
Chemistry, Multidisciplinary
Elena Scurti, Joao Pedro Martins, Christian Celia, Paola Palumbo, Francesca Lombardi, Dalila Iannotta, Luisa Di Marzio, Helder A. Santos, Tapani Viitala
Summary: In this study, the interactions between bare niosomes and chitosan-coated niosomes (chitosomes) and intestinal cells were investigated using surface plasmon resonance (SPR)-based cell sensing. The results showed that chitosomes interacted more efficiently with HT29-MTX, Caco-2, and Caco-2/HT29-MTX cocultured cells compared to bare niosomes. SPR analysis, confocal microscopy, and flow cytometry studies demonstrated that this label-free and real-time method is useful for characterizing the interactions between niosomes and in vitro cell models.
Article
Chemistry, Multidisciplinary
Pengfei Zhang, Xinyu Zhou, Rui Wang, Jiapei Jiang, Zijian Wan, Shaopeng Wang
Summary: Cell adhesion is crucial for cell functions, and plasmonic scattering microscopy (PSM) can directly image single focal adhesions with sub-micrometer spatial resolution. PSM offers high signal-to-noise ratio and enables super-resolution lateral localization and precise tracking of vertical distances for analyzing free-energy profiles. PSM provides insights into focal adhesion activities and serves as a promising tool for investigating cell/surface interactions and tissue adhesive materials screening.
Article
Chemistry, Medicinal
Kevin P. Cusack, Ying Wang, Michael Z. Hoemann, Jasmina Marjanovic, Roland G. Heym, Anil Vasudevan
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2015)
Article
Biochemistry & Molecular Biology
Franziska Theresia Edelmann, Andreas Schlundt, Roland Gerhard Heym, Andreas Jenner, Annika Niedner-Boblenz, Muhammad Ibrahim Syed, Jean-Christophe Paillart, Ralf Stehle, Robert Janowski, Michael Sattler, Ralf-Peter Jansen, Dierk Niessing
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Franziska Theresia Edelmann, Andreas Schlundt, Roland Gerhard Heym, Andreas Jenner, Annika Niedner-Boblenz, Muhammad Ibrahim Syed, Jean-Christophe Paillart, Ralf Stehle, Robert Janowski, Michael Sattler, Ralf-Peter Jansen, Dierk Niessing
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2017)
Review
Biochemistry & Molecular Biology
Roland Gerhard Heym, Dierk Niessing
CELLULAR AND MOLECULAR LIFE SCIENCES
(2012)
Article
Biochemistry & Molecular Biology
Marisa Mueller, Roland Gerhard Heym, Andreas Mayer, Katharina Kramer, Maria Schmid, Patrick Cramer, Henning Urlaub, Ralf-Peter Jansen, Dierk Niessing
Article
Neurosciences
Kerstin Schlegel, Khader Awwad, Roland G. Heym, David Holzinger, Annika Doell, Stefan Barghorn, Thomas R. Jahn, Corinna Klein, Yulia Mordashova, Michael Schulz, Laura Gasparini
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2019)
Article
Neurosciences
Marco J. Russo, Christina D. Orru, Luis Concha-Marambio, Simone Giaisi, Bradley R. Groveman, Carly M. Farris, Bret Holguin, Andrew G. Hughson, David-Erick Lafontant, Chelsea Caspell-Garcia, Christopher S. Coffey, Jennifer Mollon, Samantha J. Hutten, Kalpana Merchant, Roland G. Heym, Claudio Soto, Byron Caughey, Un Jung Kang
Summary: Alpha Synuclein seed amplification assays (Alpha Syn-SAAs) are considered promising diagnostic tools for Parkinson's disease and related synucleinopathies, showing high diagnostic accuracy in multiple patient cohorts. This study compared results from three independent laboratories and found high reproducibility and diagnostic performance of the assay.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Stephanie Unterreitmeier, Angelika Fuchs, Teresa Schaeffler, Roland G. Heym, Dmitrij Frishman, Dieter Langosch
JOURNAL OF MOLECULAR BIOLOGY
(2007)
Article
Biochemistry & Molecular Biology
Trang Van Tran, Hoa Nguyen, Luyen Vu, ChangWoo Lee
Summary: Glutaredoxin 3 (Grx3) is a redox protein that maintains structural integrity and glutathione (GSH) binding capabilities across different temperatures. This study investigates the roles of specific bonds in Grx3's structure and function, and how psychrophilic Grx3 variants adapt to cold environments. The highly conserved Arg51-Asp69 salt bridge and Gln56-His63 hydrogen bond are crucial for stabilizing the structure and catalytic activity of Grx3. Psychrophilic variants of Grx3 have adapted to cold environments by reducing GSH binding and increasing structural flexibility.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Amanda Lais de Souza Coto, Arthur Alexandre Pereira, Sabrina Dorta Oliveira, Milene Nobrega de Oliveira Moritz, Arthur Moraes Franco da Rocha, Paulo Roberto Dores-Silva, Noeli Soares Melo da Silva, Ana Rita de Araujo Nogueira, Lisandra Marques Gava, Thiago Vagas Seraphim, Julio Cesar Borges
Summary: J-domain proteins form a large molecular chaperone family involved in proteostasis processes, with hDjC20 playing a vital role in mitochondria and being heavily influenced by the presence of Zn+2.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Meiling Zhang, Jiaxiang Zhang, Yan Liang, Shicheng Tian, Shuyang Xie, Tong Zhou, Qin Wang
Summary: This study determined the crystal structures of RGLG2 VWA domain in Arabidopsis thaliana, revealing that Ca2+ ions act as regulators and affect the conformational change of RGLG2-VWA domain.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Alexandra Bork, Sander H. J. Smits, Lutz Schmitt
Summary: This study reveals the structure and calcium ion binding properties of CBL1 protein, and proposes a binding model of CBL1 for Ca2+. Additionally, it provides preliminary insights into the formation of the dimer interface of CBL1.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Evgeniia V. Leisi, Andrey V. Moiseenko, Sofia S. Kudryavtseva, Denis V. Pozdyshev, Vladimir I. Muronetz, Lidia P. Kurochkina
Summary: The pathogenesis of prion diseases involves the transformation of prion protein into an insoluble form. This study found that two phage chaperonins can promote the fibrillation of prion protein in an ATP-dependent manner, resulting in the formation of less toxic large clusters. These fibrils differ in morphology and properties from those formed spontaneously in acidic pH with denaturants.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Gaurab Chowdhury, Saroj Biswas, Yuthika Dholey, Puja Panja, Sumit Das, Subrata Adak
Summary: Magnesium is an important divalent cation for regulating enzyme activity. The binding of Mg2+ through the PAS domain inhibits phosphoglycerate kinase (PGK) activity in LmPAS-PGK at neutral pH, but PGK activity is derepressed at acidic pH. Mutation studies revealed that the Asp-4 residue is crucial for Mg2+ binding at neutral pH.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Shima Ghaedizadeh, Majid Zeinali, Bahareh Dabirmanesh, Behnam Rasekh, Khosrow Khajeh, Ali Mohammad Banaei-Moghaddam
Summary: Implementing hyperthermostable carbonic anhydrases into CO2 capture and storage technologies can increase the rate of CO2 absorption from industrial flue gases. This study successfully improved the thermostability of a known hyperthermostable carbonic anhydrase through rational engineering of a single-point mutation.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Khaled A. Elnahriry, Dorothy C. C. Wai, Lauren M. Ashwood, Muhammad Umair Naseem, Tibor G. Szanto, Shaodong Guo, Gyorgy Panyi, Peter J. Prentis, Raymond S. Norton
Summary: Sea anemone venom contains a peptide called Tst2, which shows sequence similarity to peptides that interact with various ion channels. Recombinant Tst2 was successfully produced and its structure and function were studied. The results showed that Tst2 is an inhibitor of the TRPV1 channel.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)