Article
Biochemistry & Molecular Biology
Enrico C. Torre, Mesude Bicer, Graeme S. Cottrell, Darius Widera, Francesco Tamagnini
Summary: Adipose-derived mesenchymal stromal cells (ASCs) have the potential to differentiate into various cell types and exhibit spontaneous Ca2+ oscillations. The effects of differentiation treatments on ASCs' Ca2+ dynamics, in terms of oscillation fraction and frequency, were examined in this study. Both adipogenic and osteogenic differentiation resulted in reduced Ca2+ dynamics, with adipogenic differentiation showing a more pronounced effect. Changes in Ca2+ oscillations were observed as early as 7 days in vitro (DIV). Further research is needed to understand the molecular and mechanistic correlates of these changes for the development of interventions in regenerative medicine.
Article
Biochemistry & Molecular Biology
Jiang Liu, Xin-Tong Yao, Xiao-Lei Feng, Xi-Zhuang Bai
Summary: In recent years, there has been increasing attention on bone loss related diseases, such as osteoporosis and osteonecrosis of the femoral head, which exhibit symptoms of insufficient bone mass. Mesenchymal stem cells (MSCs) hold potential as a new solution for bone disease, as they can be induced to differentiate into osteoblasts. This study investigates the mechanism by which BMP2 drives MSCs to differentiate into osteoblasts through ACKR3/p38/MAPK signaling. The findings suggest that ACKR3 could be a novel therapeutic target for bone-associated diseases and bone-tissue engineering.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Cell Biology
Bo Jia, Jun Chen, Qin Wang, Xiang Sun, Jiusong Han, Fernando Guastaldi, Shijian Xiang, Qingsong Ye, Yan He
Summary: The study reveals that SIRT6 physically interacts with DNMT1 protein, deacetylating and destabilizing it, leading to the activation of NOTCH1 and NOTCH2, which promotes the osteogenic differentiation of ADSCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Baojun Sun, Yajie Sun, Shuwei Han, Ruitong Zhang, Xiujuan Wang, Chunxia Meng, Tuo Ji, Chunhui Sun, Na Ren, Shaohua Ge, Hong Liu, Yang Yu, Jingang Wang
Summary: Traditional bone defect treatments face limitations due to insufficient supply of autologous bone, immune rejection of allogeneic bone grafts, and high medical costs. To overcome these limitations, bone tissue engineering with electroactive scaffolds has emerged as a promising option. However, current electroactive scaffolds are either single-function and not suitable for scientific research and clinical application, or complex and expensive to prepare. This study proposes a new tissue engineering strategy to optimize the electrical properties and biocompatibility of carbon-based materials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Tianyu Chen, Dexuan Xiao, Yanjing Li, Sirong Shi, Xiao Yang, Shuanglin Peng, Bin Guo, Xiaoxiao Cai
Summary: Osteoporosis has a severe impact on the health of elderly individuals, but autologous stem-cell transplantation can help improve bone density and fracture healing abilities. However, osteoporosis can negatively affect the osteogenesis and proliferation abilities of autologous adipose-derived stem cells. A study found that tetrahedral framework nucleic acid can effectively promote the recovery of osteogenic and proliferative potential in osteoporotic ASCs and enhance their bone formation and proliferation.
CHINESE CHEMICAL LETTERS
(2022)
Article
Engineering, Biomedical
Sujee Jeyapalina, Elaine Hillas, James Peter Beck, Jayant Agarwal, Jill Shea
Summary: This study aimed to investigate the potential benefits of FA and FHA scaffolds in conjunction with ADSCs for bone regeneration. The results showed that FA and FHA surfaces could drive ADSCs towards osteogenic differentiation earlier, potentially improving bone regeneration following injury.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Daowen Luo, Shuanglin Peng, Qing Li, Pengcheng Rao, Gang Tao, Lang Wang, Jingang Xiao
Summary: This study found that the expression of METTL3 decreased in adipose-derived stem cells (ASCs) from osteoporotic rats (OP-ASCs), leading to a decrease in their osteogenic ability. Overexpression of METTL3 could restore the osteogenic capacity of OP-ASCs, providing a new target for treating osteoporotic bone defects.
JOURNAL OF GENE MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Bing Sun, Rongmei Qu, Tingyu Fan, Yuchao Yang, Xin Jiang, Asmat Ullah Khan, Zhitao Zhou, Jingliao Zhang, Kuanhai Wei, Jun Ouyang, Jingxing Dai
Summary: This study suggests that the actin polymerization state may promote osteogenic differentiation of hASCs by regulating the protein expression of focal adhesion-associated proteins in a concentration-dependent manner. The findings provide valuable information for exploring the mechanism of osteogenic differentiation in hASCs.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Bin Ren, Oliver B. Betz, Daniel Seitz, Christian Thirion, Michael Salomon, Volkmar Jansson, Peter E. Mueller, Volker M. Betz
Summary: This study evaluated a novel growth factor delivery system, consisting of adenoviral BMP-2 transduced fascia tissue, for its osteogenic effects on hADSCs seeded on an innovative biomimetic spongiosa-like scaffold. The results showed that delivery of BMP-2 by overexpressing fascia provided a continuous and uniform level of BMP-2 over a period of 30 days, leading to a strong osteogenic response of hADSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Wentian Sun, Yidan Song, Kai Xia, Liyuan Yu, Xinqi Huang, Zhihe Zhao, Jun Liu
Summary: A transcriptome-wide m(6)A methylome was built during the osteogenic differentiation of human adipose-derived stem cells (hASCs), revealing the influence of m(6)A on various signaling pathways and hub genes related to bone regeneration. Further epigenetic studies based on these findings could enhance the understanding of the mechanisms underlying hASCs osteogenic differentiation.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Orthopedics
Parisa Kangari, Leila Roshangar, Aida Iraji, Tahereh Talaei-Khozani, Mahboobeh Razmkhah
Summary: Shilajit promotes osteogenic differentiation of human adipose-derived mesenchymal stem cells in both 3D and 2D cultures. The combination of Shilajit and Alg hydrogel initiates osteogenic differentiation in a short period of time.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Saideh Hemati, Ashrafalsadat Hatamian-Zarmi, Raheleh Halabian, Mohsen Ghiasi, Ali Salimi
Summary: The research indicates that SPG can effectively promote osteogenic differentiation of hADMSCs, enhance osteogenic markers and mineralization, and upregulate the expression of bone-specific genes.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Engineering, Environmental
Zhuo Liang, Li Yang, Yonggang Lv
Summary: A novel gene delivery vector was developed using a layer-by-layer self-assembly method with PEI and Exos derived from MSCs, which exhibited high transfection efficiency and low cytotoxicity to MSCs. Additionally, a hypoxia-specific expression plasmid combining hypoxia promoter RTP801 and miR877 sponge significantly increased BMP2 expression in hypoxic conditions and promoted neoangiogenesis, providing a potential method to enhance bone repair in tissue engineering.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Cell Biology
Pengcheng Rao, Fangzhi Lou, Daowen Luo, Chenglong Huang, Kui Huang, Zhihao Yao, Jingang Xiao
Summary: The study revealed the crucial roles of autophagy and Notch signaling pathway in the osteogenic differentiation of adipose-derived stem cells (ASCs) in type 2 diabetic osteoporosis (Dop) mice. Downregulated autophagy suppressed Notch signaling, leading to reduced osteogenic capacity, which can be rescued by Torin1 through activation of autophagy.
CELLULAR SIGNALLING
(2021)
Article
Cell & Tissue Engineering
Daniella Da Silva, Anine Crous, Heidi Abrahamse
Summary: Osteoporosis is a common degenerative bone condition, and stem cell regenerative therapy and photobiomodulation have shown promise in treating it. This study found that a combination of photobiomodulation and differentiation inducers stimulated the early osteogenic differentiation of stem cells. However, further research is needed to establish the effectiveness of this novel wavelength combination.
REGENERATIVE THERAPY
(2023)