Article
Biotechnology & Applied Microbiology
Yuwen Zhao, Kiarra Richardson, Rui Yang, Zoe Bousraou, Yoo Kyoung Lee, Samantha Fasciano, Shue Wang
Summary: Osteoporosis is a common bone and metabolic disease characterized by bone density loss and microstructural degeneration. This study investigates the regulatory role of Notch signaling during osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hMSCs) exposed to physiologically relevant fluid shear stress using a locked nucleic acid (LNA)/DNA nanobiosensor. The results provide evidence that shear stress regulates osteogenic differentiation through Notch signaling.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Pengcheng Rao, Fangzhi Lou, Daowen Luo, Chenglong Huang, Kui Huang, Zhihao Yao, Jingang Xiao
Summary: The study revealed the crucial roles of autophagy and Notch signaling pathway in the osteogenic differentiation of adipose-derived stem cells (ASCs) in type 2 diabetic osteoporosis (Dop) mice. Downregulated autophagy suppressed Notch signaling, leading to reduced osteogenic capacity, which can be rescued by Torin1 through activation of autophagy.
CELLULAR SIGNALLING
(2021)
Article
Biochemistry & Molecular Biology
Aleksandra Kostina, Arseniy Lobov, Daria Semenova, Artem Kiselev, Polina Klausen, Anna Malashicheva
Summary: Despite significant progress in bone tissue regeneration, the early mechanisms of osteogenic differentiation remain poorly understood. Various cells can undergo osteogenic transformation, with Notch signaling playing a key role in this process. Research demonstrates differences in the ability of different cell types to undergo Notch-dependent osteogenic differentiation.
Article
Veterinary Sciences
Uffaq Shabir, Irfan Ahmad Bhat, Bilal Ahmad Pir, Mukesh Kumar Bharti, Sriti Pandey, Sai Kumar Gutulla, Mihir Sarkar, Yasotha Thirupathi, Vikash Chandra, Arvind Sonewane, Taru Sharma Gutulla
Summary: This study investigates the roles of bone morphogenetic protein (BMP) and Notch signaling pathways in the differentiation of canine bone marrow-derived MSCs by silencing specific genes. The results show that Runx2 plays an essential role in directing MSCs towards osteogenic lineage, while Smad-mediated BMP signaling induces osteoblast-specific gene expression and the Notch pathway negatively regulates osteogenic differentiation of canine MSCs.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Enrico C. Torre, Mesude Bicer, Graeme S. Cottrell, Darius Widera, Francesco Tamagnini
Summary: Adipose-derived mesenchymal stromal cells (ASCs) have the potential to differentiate into various cell types and exhibit spontaneous Ca2+ oscillations. The effects of differentiation treatments on ASCs' Ca2+ dynamics, in terms of oscillation fraction and frequency, were examined in this study. Both adipogenic and osteogenic differentiation resulted in reduced Ca2+ dynamics, with adipogenic differentiation showing a more pronounced effect. Changes in Ca2+ oscillations were observed as early as 7 days in vitro (DIV). Further research is needed to understand the molecular and mechanistic correlates of these changes for the development of interventions in regenerative medicine.
Review
Biochemistry & Molecular Biology
Chiara Mazziotta, Carmen Lanzillotti, Maria Rosa Iaquinta, Francesca Taraballi, Elena Torreggiani, John Charles Rotondo, Lucia Oton-Gonzalez, Elisa Mazzoni, Francesca Frontini, Ilaria Bononi, Monica De Mattei, Mauro Tognon, Fernanda Martini
Summary: MSCs are important source for bone regeneration and play a crucial role in osteogenic differentiation. MiRNAs regulate gene expression in osteogenic differentiation of MSCs and are involved in controlling important signaling pathways such as TGF-beta/BMP and Wnt/beta-catenin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yukun Mao, Na Ni, Linjuan Huang, Jiaming Fan, Hao Wang, Fang He, Qing Liu, Deyao Shi, Kai Fu, Mikhail Pakvasa, William Wagstaff, Andrew Blake Tucker, Connie Chen, Russell R. Reid, Rex C. Haydon, Sherwin H. Ho, Michael J. Lee, Tong-Chuan He, Jian Yang, Le Shen, Lin Cai, Hue H. Luu
Summary: The study shows that BMP9 can induce osteogenic and adipogenic differentiation of MSCs by upregulating the expression of AGO proteins. Silencing individual AGO expression leads to a decrease in osteogenic marker activity, while silencing all four AGO genes significantly diminishes BMP9-induced differentiation and bone formation of MSCs. AGO proteins and small RNA biogenesis pathway play an essential role in mediating BMP9-induced osteogenic differentiation of MSCs.
Article
Materials Science, Multidisciplinary
Jong Bo Park, Ji Yeon Ahn, Woo Sub Yang, Seong Chae Park, Young Jin Jung, Myungook Lee, Byung Hee Hong, Jeong Mook Lim
Summary: This study investigated the effect of nanoscale spacing by stacked graphene film on osteogenic differentiation of human adipose-derived mesenchymal stromal cells. The results showed that osteogenic differentiation was promoted as the stacking layer increased, with the most significant effects observed in the three layers. The study demonstrated that multilayer-stacked graphene created nanoscale spaces promote calcium deposit and cytoskeletal integrity in hADSC-related, in vitro-osteogenesis.
Article
Biochemistry & Molecular Biology
Baojun Sun, Yajie Sun, Shuwei Han, Ruitong Zhang, Xiujuan Wang, Chunxia Meng, Tuo Ji, Chunhui Sun, Na Ren, Shaohua Ge, Hong Liu, Yang Yu, Jingang Wang
Summary: Traditional bone defect treatments face limitations due to insufficient supply of autologous bone, immune rejection of allogeneic bone grafts, and high medical costs. To overcome these limitations, bone tissue engineering with electroactive scaffolds has emerged as a promising option. However, current electroactive scaffolds are either single-function and not suitable for scientific research and clinical application, or complex and expensive to prepare. This study proposes a new tissue engineering strategy to optimize the electrical properties and biocompatibility of carbon-based materials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Shiva Shariati, Ehsan Seyedjafari, Fatemeh Sadat Mahdavi, Amirhosein Maali, Elaheh Ferdosi-Shahandashti
Summary: In this study, NiFe2O4/ZnO-coated PLLA scaffold was used to evaluate the osteogenic differentiation capability of hAMSCs. The results showed that the NiFe2O4/ZnO-coated PLLA scaffold could enhance the adhesion, proliferation, and differentiation of hAMSCs, as well as promote the expression of osteogenic markers.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Orthopedics
Yu-hu Zhou, Qiang Xie
Summary: The study showed that total glycosides from Eucommia ulmoides seed (TGEUS) can enhance osteogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs) and promote bone formation in ovariectomy-induced bone loss rats. Treatment with TGEUS resulted in significantly increased trabecular bone volume and thickness in rats, along with elevated expression of osteogenic markers.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2021)
Article
Orthopedics
Parisa Kangari, Leila Roshangar, Aida Iraji, Tahereh Talaei-Khozani, Mahboobeh Razmkhah
Summary: Shilajit promotes osteogenic differentiation of human adipose-derived mesenchymal stem cells in both 3D and 2D cultures. The combination of Shilajit and Alg hydrogel initiates osteogenic differentiation in a short period of time.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Ying Xing, Mi-Shuai Zhang, Jian-Hui Xiao, Ru-Ming Liu
Summary: This study found that galangin can promote the osteogenic differentiation of human amniotic mesenchymal stem cells (hAMSCs) through the JAK2/STAT3 signaling pathway.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Yuwen Zhao, Rui Yang, Zoe Bousraou, Kiarra Richardson, Shue Wang
Summary: This study established a LNA/DNA nanobiosensor for gene expression analysis in single hMSCs during osteogenic differentiation. The results showed that Notch1-Dll4 signaling plays an important role in regulating hMSCs osteogenic differentiation. Additionally, Dll4 was identified as a molecular signature of differentiated hMSCs during osteogenic induction, and the role of Notch1-Dll4 signaling in a 3D spheroid model was elucidated.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Saideh Hemati, Ashrafalsadat Hatamian-Zarmi, Raheleh Halabian, Mohsen Ghiasi, Ali Salimi
Summary: The research indicates that SPG can effectively promote osteogenic differentiation of hADMSCs, enhance osteogenic markers and mineralization, and upregulate the expression of bone-specific genes.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Medicine, Research & Experimental
Xianghuai Zheng, Bo Jia, Xi Lin, Jiusong Han, Xiaoling Qiu, Hongxing Chu, Xiang Sun, Weitao Hu, Jie Pan, Jun Chen, Jianjiang Zhao
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2016)
Article
Dentistry, Oral Surgery & Medicine
Jianjiang Zhao, Zhiping Wang, Jiusong Han, Xiaoling Qiu, Jie Pan, Jun Chen
CLINICAL ORAL INVESTIGATIONS
(2014)
Article
Biotechnology & Applied Microbiology
Jianjiang Zhao, Xiaoling Qiu, Zhiping Wang, Jie Pan, Jun Chen, Jiusong Han
ONCOTARGETS AND THERAPY
(2013)
Article
Oncology
Bo Jia, Junfeng Dao, Jiusong Han, Zhijie Huang, Xiang Sun, Xianghuai Zheng, Shijian Xiang, Huixi Zhou, Shuguang Liu
Summary: LINC00958 expression is increased in TSCC tissues, with high expression associated with shorter overall survival. Knockdown of LINC00958 significantly reduces the growth rate of TSCC cells, acting as a ceRNA by competitively sponging miR-211-5p. CENPK, a direct target gene of miR-211-5p, promotes cell proliferation in TSCC.
CANCER CELL INTERNATIONAL
(2021)
Article
Biotechnology & Applied Microbiology
Xiang Sun, Junchuan Cao, Jiusong Han, Bo Jia, Jing Wang, Junxiang Lian, Jianwei Gao, Shuguang Liu, Hui Xiao
Summary: The study investigated the role of lncRNA RP11-815M8.1 in the osteogenic differentiation of human bone marrow mesenchymal stem cells. Results showed that the expression of RP11-815M8.1 increased with osteogenic differentiation time and overexpression of RP11-815M8.1 promoted osteogenic differentiation while knockdown inhibited it.
BIOMED RESEARCH INTERNATIONAL
(2021)