Article
Biochemistry & Molecular Biology
Mengyi Wei, Jindun Zhang, Jia Liu, Chaoyue Zhao, Shuo Cao, Xiaojie Yan, Baojiang Wu, Siqin Bao
Summary: Parthenogenetic embryos have been studied extensively for their potential in generating various stem cell lines with distinct characteristics compared to traditional pluripotent stem cells. By manipulating the culture medium components, researchers were able to demonstrate the conversion from primed state to advanced state stem cells, showing enhanced developmental potential.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Kenta Nishitani, Koji Hayakawa, Satoshi Tanaka
Summary: The role of EGF signaling in mouse trophoblast differentiation was investigated using mouse trophoblast stem cells (mTSCs) in an in vitro culture system. EGF stimulation at the early stage of differentiation repressed mTSC differentiation into spongiotrophoblast cells (SpT) through the EGF-Egfr-MAPK/ERK-Mash2 axis. Inhibition of the MAPK/ERK pathway alleviated the EGF-mediated repression of SpT differentiation. These findings provide insights into the molecular mechanisms underlying trophoblast cell fate decisions.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Kenta Nishitani, Koji Hayakawa, Masayuki Minatomoto, Keisuke Tanaka, Hidehiko Ogawa, Hirotatsu Kojima, Satoshi Tanaka
Summary: This study investigated the molecular mechanisms of trophoblast cell differentiation using mouse trophoblast stem cells. The researchers identified N-oleoyldopamine (OLDA) as a compound that promotes the differentiation of trophoblast cells into parietal trophoblast giant cells and represses their differentiation into spongiotrophoblast cells. They also discovered that OLDA regulates the expression of Fosl1, a key regulatory factor in trophoblast cell differentiation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Genetics & Heredity
Xuhui Kong, Xin Chen, Songbang Ou, Wenjun Wang, Ruiqi Li
Summary: This study established triploid human trophoblast stem cell lines derived from tripronuclear embryos, providing a useful in vitro model for studying human placental biology and disease.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)
Article
Cell & Tissue Engineering
Bryony V. V. Natale, Ramie Kotadia, Katarina Gustin, Anirudha Harihara, Sarah Min, Michael J. J. Kreisman, Kellie M. M. Breen, David R. C. Natale
Summary: This study identified the roles of different extracellular matrix (ECM) components in the maintenance and differentiation of trophoblast stem (TS) cells. Laminin promotes the expression of markers associated with undifferentiated TS cells, fibronectin promotes the expression of markers associated with syncytiotrophoblast layer II cells, and collagen IV promotes the expression of markers associated with differentiated trophoblast.
STEM CELLS AND DEVELOPMENT
(2023)
Article
Multidisciplinary Sciences
Yu Jin Jang, Mijeong Kim, Bum-Kyu Lee, Jonghwan Kim
Summary: The authors developed a simple procedure to convert human primed pluripotent stem cells into trophoblast stem-like cells using bone morphogenetic protein 4, providing an important opportunity to study human placenta development and pathology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Wei Yu, V. Praveen Chakravarthi, Shaon Borosha, Iman Dilower, Eun Bee Lee, Anamika Ratri, Rebekah R. Starks, Patrick E. Fields, Michael W. Wolfe, M. Omar Faruque, Geetu Tuteja, M. A. Karim Rumi
Summary: The study reveals that trophoblast-specific Satb1 expression is regulated by long-range chromatin looping mediated by an enhancer that interacts with ELF5 and SATB proteins, contributing to the maintenance of cell state.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Noura N. Ballasy, Elizabeth A. Bering, Caroline Kokorudz, Bethany N. Radford, Xiang Zhao, Wendy Dean, Myriam Hemberger
Summary: Histone citrullination, mediated by PADIs, plays a critical epigenetic role in safeguarding the stem cell state of mouse trophoblast stem cells (TSCs) by modulating the DNA methylation landscape to restrict precocious trophoblast differentiation.
Article
Cell & Tissue Engineering
Zhuoran Yu, Sihan Zhou, Minli Liu, Lijun Chen, Xue Zhang, Xu Yang, Hongdi Cui, Chaoqian Jiang, Yanjiao Lv, Tingsheng Yan, Qingran Kong, Zhonghua Liu
Summary: This study successfully derived and maintained stable porcine pluripotent stem cells (pLCDMs) using the LCDM culture system. Transcriptome analysis revealed the unique molecular characteristics and similarities/differences with human and mouse EPSCs. Furthermore, two small molecules in the culture system were found to play a crucial role in promoting trophoblast lineage differentiation potential of pLCDMs.
Article
Biochemistry & Molecular Biology
Shadi Tarazi, Alejandro Aguilera-Castrejon, Carine Joubran, Nadir Ghanem, Shand Ashouokhi, Francesco Roncato, Emilie Wildschutz, Montaser Haddad, Bernardo Oldak, Elidet Gomez-Cesar, Nir Livnat, Sergey Viukov, Dmitry Lokshtanov, Segev Naveh-Tassa, Max Rose, Suhair Hanna, Calanit Raanan, Ori Brenner, Merav Kedmi, Hadas Keren-Shaul, Tsvee Lapidot, Itay Maza, Noa Novershtern, Jacob H. Flannel
Summary: Researchers have successfully generated synthetic whole embryo models with both embryonic and extraembryonic compartments using manipulated naive pluripotent stem cells. These models can complete embryonic development, develop organ progenitors, and exhibit complex structures similar to mouse embryos. This discovery highlights the potential of naive pluripotent cells.
Article
Biochemistry & Molecular Biology
Xiaopeng Hu, Hu Wang, Geng G. Tian, Changliang Hou, Bo Xu, Xinyan Zhao, Yongqiang Zhao, Qian Fang, Xinyue Li, Lin He, Xuejin Chen, Shangang Li, Ji Wu
Summary: This study demonstrates that sperm can be produced from FGSCs without a Y chromosome. This suggests a strategy for dairy cattle breeding to produce only female offspring with a high-quality genetic background.
CELL AND BIOSCIENCE
(2022)
Article
Obstetrics & Gynecology
Mary McKnight, Sherina Lawrence, Philip Xie, Zev Rosenwaks, Gianpiero Palermo
Summary: De novo male gametes capable of inducing full preimplantation blastocyst development can be obtained using a novel three-dimensional (3D) culture system. Mouse embryonic stem cells (mESCs) were differentiated into male germ-like cells in a 3D environment that simulates the seminiferous tubule. However, the functional gametes generated still had some differences compared to mature spermatozoa.
FERTILITY AND STERILITY
(2023)
Article
Biochemical Research Methods
Shao Xu, Sidong Wang, Timothy Theodore Ka Ki Tam, Pengtao Liu, Degong Ruan
Summary: This article presents a detailed protocol for differentiating human expanded potential stem cells (hEPSCs) into human trophoblast stem cells (TSCs) and establishing TSC lines. The hEPSC-derived TSC lines can be continuously passaged and are capable of further differentiation into syncytiotrophoblasts and extravillous trophoblasts. The hEPSC-TSC system provides a valuable cell source for studying human trophoblast development in pregnancy.
Article
Developmental Biology
Yutong Chen, Dylan Siriwardena, Christopher Penfold, Adam Pavlinek, Thorsten E. Boroviak
Summary: This study provides a single-cell atlas of human trophoblast development and performs single-cell profiling of trophoblast stem cells. The study reveals the transcriptional networks of trophoblast lineages and identifies the role of MAPK signaling in cell differentiation. In addition, the study identifies key factors regulating self-renewal of trophoblast stem cells.
Article
Cell Biology
Weisheng Zheng, Liping Wang, Wenteng He, Xinjie Hu, Qianshu Zhu, Liang Gu, Cizhong Jiang
Summary: Haploid embryonic stem cells (haESCs) have similar transcriptome profiles to naive pluripotent stem cells and exhibit a similar chromatin structure to embryonic stem cells (ESCs). However, haESCs lack a nucleosome depletion region found in ESCs and have different chromatin states in enhancers related to the regulation of the cell cycle. These findings suggest that haESCs have unique characteristics and mechanisms compared to differentiated cell types.
CELL PROLIFERATION
(2023)
Article
Cell Biology
Rui Fu, Dawei Yu, Jilong Ren, Chongyang Li, Jing Wang, Guihai Feng, Xuepeng Wang, Haifeng Wan, Tianda Li, Libin Wang, Ying Zhang, Tang Hai, Wei Li, Qi Zhou
Article
Cell Biology
Jing Li, Chenxin Wang, Guihai Feng, Linlin Zhang, Guilai Chen, Hao Sun, Jiaqiang Wang, Ying Zhang, Qi Zhou, Wei Li
CELL PROLIFERATION
(2020)
Article
Immunology
Rui Fu, Minghui Fang, Kai Xu, Jilong Ren, Jun Zou, Long Su, Xinxin Chen, PeiPei An, Dawei Yu, Meina Ka, Tang Hai, Ziyi Li, Wei Li, Yongguang Yang, Qi Zhou, Zheng Hu
Article
Biochemistry & Molecular Biology
Bin Qu, Shijing Wu, Guanyi Jiao, Xuan Zou, Zhikun Li, Lu Guo, Xuehan Sun, Cheng Huang, Zixi Sun, Ying Zhang, Hui Li, Qi Zhou, Ruifang Sui, Wei Li
Letter
Cell Biology
Junjie Mao, Kai Xu, Jiabao Han, Guihai Feng, Ying Zhang, Wei Li
Letter
Cell Biology
Lu Guo, Xuehan Sun, Xinge Wang, Chen Liang, Haiping Jiang, Qingqin Gao, Moyu Dai, Bin Qu, Sen Fang, Yihuan Mao, Yangcan Chen, Guihai Feng, Qi Gu, Ruiqi Rachel Wang, Qi Zhou, Wei Li
Article
Cell Biology
Haifeng Wan, Rui Fu, Man Tong, Yukai Wang, Libin Wang, Siqi Wang, Ying Zhang, Wei Li, Xiu-Jie Wang, Guihai Feng
Summary: The study revealed the impact of feeder cell residues on the transcriptomic data analysis of hPSCs, providing solutions to minimize the effects.
CELL PROLIFERATION
(2022)
Article
Biochemistry & Molecular Biology
Jilong Ren, Dawei Yu, Jing Wang, Kai Xu, Yanan Xu, Renren Sun, Peipei An, Chongyang Li, Guihai Feng, Ying Zhang, Xiangpeng Dai, Hongye Zhao, Zhengzhu Wang, Zhiqiang Han, Haibo Zhu, Yuchun Ding, Xiaoyan You, Xueqin Liu, Meng Wu, Lin Luo, Ziyi Li, Yong-Guang Yang, Zheng Hu, Hong-Jiang Wei, Liangpeng Ge, Tang Hai, Wei Li
Summary: We successfully generated pig models with severe immunodeficiency that could construct human liver tissues.
CELL AND BIOSCIENCE
(2022)
Article
Cell Biology
Jiabao Han, Liyu Zhu, Jingwen Zhang, Lu Guo, Xuehan Sun, Cheng Huang, Kai Xu, Ying Zhang, Wei Li, Qi Zhou
Summary: Our study proposes a new engineering strategy for AAV capsids and generates liver-tropic AAV variants with enhanced transduction efficiency and reduced sensitivity to neutralizing antibodies. These variants may expand the gene delivery toolbox and improve the success of AAV gene therapy.
CELL PROLIFERATION
(2022)
Article
Multidisciplinary Sciences
Li-Bin Wang, Zhi-Kun Li, Le-Yun Wang, Kai Xu, Tian-Tian Ji, Yi-Huan Mao, Si-Nan Ma, Tao Liu, Cheng-Fang Tu, Qian Zhao, Xu-Ning Fan, Chao Liu, Li-Ying Wang, You-Jia Shu, Ning Yang, Qi Zhou, Wei Li
Summary: This study reports successful programmed chromosome ligation in mice, leading to the creation of new karyotypes. By fusing chromosomes 1 and 2, as well as chromosomes 4 and 5, using haploid embryonic stem cells and gene editing, the researchers were able to generate new chromosome combinations. The fused chromosome pairs had minimal impact on chromatin conformation and stem cell differentiation. However, the fused chromosomes 1 and 2 resulted in mitotic arrest, polyploidization, and embryonic lethality, while the smaller fused chromosome composed of chromosomes 4 and 5 could be passed on to homozygous offspring.
Letter
Cell Biology
Jing Wang, Jilong Ren, Qingwei Wang, Chongyang Li, Zhiqiang Han, Tianzhi Chen, Ke Sun, Guihai Feng, Ying Zhang, Jianyong Han, Qi Zhou, Wei Li, Dawei Yu, Tang Hai
CELL PROLIFERATION
(2023)
Article
Cell Biology
Ying Wang, Ning Yang, Wen Sun, Chen Zhao, Xiaoxuan Hu, Shan Lu, Shiwei Cao, Nannan Wang, Tang Hai, Guihai Feng, Chenrui An, Haoyi Wang
Summary: Human extended pluripotent stem cells (hEPSCs), derived from primed human embryonic stem cells (hESCs), have the potential to differentiate into both embryonic and extraembryonic lineages and form human blastoids. We characterized the X chromosome status of hEPSCs and found that it influenced their ability to differentiate. This provides important information for the future application of hEPSCs.
CELL PROLIFERATION
(2023)
Article
Biochemistry & Molecular Biology
Sunyang Ying, Pengcheng Li, Jiaqiang Wang, Kaiqiong Chen, Yu Zou, Moyu Dai, Kai Xu, Guihai Feng, Changjian Zhang, Haiping Jiang, Wei Li, Ying Zhang, Qi Zhou
Summary: tsRNAs, as products of the stress response, play a vital role in stress response and injury regulation. This study demonstrates the potential of tsRNAs in alleviating liver injury by utilizing the loss of NSun2 as a tsRNAs-generating model. It is shown that tsRNAs can repress global protein synthesis and ameliorate liver injury through the weakened association between TSR1 and pre-40S ribosome.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
