Article
Multidisciplinary Sciences
Guopu Chen, Fengyuan Wang, Min Nie, Hui Zhang, Han Zhang, Yuanjin Zhao
Summary: The newly developed roe-inspired microcapsules, providing sufficient nutrient supply and maintaining the functions of mesenchymal stem cells through core-shell structure, have the potential for clinical application in treating inflammatory bowel disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Alena Wulf, Rais Mendgaziev, Rawil Fakhrullin, Vladimir Vinokurov, Dmitry Volodkin, Anna S. Vikulina
Summary: One of the main challenges in modern tissue engineering is designing biocompatible scaffolds with finely tuned porous architecture and the ability to load bioactive molecules that guide cell growth and differentiation. This study proposes a strategy to design porous alginate scaffolds by leaching sacrificial vaterite CaCO3 microspheres packed in alginate, resulting in well-tuned architecture. The pore size and interconnectivity of the scaffold depend on the dimensions and packing of the CaCO3 spheres, as well as the concentration of alginate. By varying these parameters, it is possible to achieve almost complete pore interconnectivity or no interconnectivity at all. The mechanical properties of the scaffolds are suitable for cell seeding, with the potential for encapsulation of macromolecules in individual pores to create a multifunctional scaffold. Culturing different cell lines on these scaffolds showed promising results in terms of cell viability and adhesiveness.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Engineering, Biomedical
Zeyang Liu, Hongyong Zhang, Zhen Zhan, Haochen Nan, Nan Huang, Tao Xu, Xiaohua Gong, Chengzhi Hu
Summary: Internal gelation has been improved to prepare core-shell hydrogel microcapsules with high mechanical stability and cell survival rate. A coaxial flow-focusing microfluidic device was developed to facilitate the gelation process, resulting in well-defined and homogenous alginate hydrogel microstructures. High acid concentration in the oil solution during gelation allows for better crosslinking of the alginate while maintaining high cell viability.
Review
Materials Science, Biomaterials
Bruno M. Ladeira, Catarina A. Custodio, Joao F. Mano
Summary: Core-shell microcapsules have the potential to recreate the hierarchical structure of biological systems by independently modulating the composition of the core and shell layers. They can be tailored to recreate specific cell niches.
BIOMATERIALS SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Gabriella Teti, Eleonora Mazzotti, Valentina Gatta, Francesca Chiarini, Maria Laura Alfieri, Mirella Falconi
Summary: Osteoarthritis (OA) is a chronic degenerative disease characterized by the loss of articular cartilage. Senescent cells in mesenchymal stem/stromal cells (MSCs) isolated from OA joints have been shown to inhibit cartilage regeneration. However, the connection between cellular senescence in MSCs and OA progression is still debated. This study aimed to characterize and compare synovial fluid MSCs (sf-MSCs) from OA joints with healthy sf-MSCs, and investigate the influence of senescence on cartilage repair. The findings demonstrated the presence of senescent sf-MSCs in OA joints, impairing chondrogenic differentiation abilities and potentially impacting OA progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biophysics
Li Wang, Bo-Bo Zhang, Xiao-Yu Yang, Bao-Lian Su
Summary: Whole-cell biocatalysis shows great promise in chemical, pharmaceutical, and fuel industries due to its high enantioselectivity, environment friendly nature, and mild reaction conditions. However, low productivity and poor stability still hinder its wide applications. In this study, hybrid alginate@polydopamine@SiO2 microcapsules with controlled porosity were developed to encapsulate yeast cells for asymmetric biosynthesis. The hybrid microcapsules demonstrated enhanced biocatalytic productivity, stability, and reusability, making them a significant advancement in cell-encapsulating materials for biocatalytic asymmetric synthesis industry.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Food Science & Technology
Laura G. Gomez-Mascaraque, Vincent Chambon, Kata Trifkovic, Andre Brodkorb
Summary: This study investigates the feasibility of producing alginate microcapsules by directly extruding and dripping them onto bovine milk. Three types of milk (skimmed, whole, and enriched milk) were used as a calcium source for capsule formation. The study analyzed the impact of milk type and properties, as well as alginate type and concentration, on the formation of capsules. The results showed that milk properties, particularly viscosity, influenced the deformation of microcapsules more than when using a CaCl2 solution. More concentrated alginate solutions and G-rich alginate resulted in more spherical capsules. The study also observed a decrease in soluble calcium and total protein content in milk after microcapsule production, indicating interactions with the alginate network
FOOD STRUCTURE-NETHERLANDS
(2023)
Article
Engineering, Biomedical
Xi Liang, Li Xie, Qingyuan Zhang, Ge Wang, Siyuan Zhang, Mingyan Jiang, Ruitao Zhang, Ting Yang, Xingyu Hu, Ziyang Yang, Weidong Tian
Summary: This study successfully fabricated GelMA-alginate core-shell microcapsules using a novel preparation technique, and co-cultured human dental pulp stem cells (hDPSCs) and human umbilical vein endothelial cells (HUVECs) in them. In the microcapsules, the co-culture group showed higher cell proliferation rates and the formation of 3D capillary-like networks. Furthermore, the co-culture group exhibited enhanced osteo/odontogenic differentiation. In vivo experiments confirmed better microvessel formation and pulp-like tissue regeneration in the co-culture group.
ACTA BIOMATERIALIA
(2022)
Article
Biochemistry & Molecular Biology
Yuxiao Wang, Chen Tan, Seyed Mohammad Davachi, Peilong Li, Philip Davidowsky, Bing Yan
Summary: In this study, an encapsulation system was developed using polyelectrolyte complexes to protect hydrophilic compounds in food or pharmaceutical applications. The method achieved good encapsulation efficiency and retention efficiency under optimized conditions, providing a simple route to efficiently encapsulate a wide range of hydrophilic ingredients.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Engineering, Biomedical
Matthew Jorgensen, Ashley Gibbons, Kevin Sui, Rebecca Carpenter, Xulang Zhang, Yubing Xie
Summary: This study developed needle-in-needle devices for fabricating stable alginate hydrogel microtubes to address challenges in organoid generation. By using a reverse engineering approach to determine optimal processing parameters and establishing a mathematical model, the research showed that the fabrication of pre-made hydrogel microtubes is more convenient, and can effectively support cell culture and research.
Article
Biotechnology & Applied Microbiology
Yiqun Ma, Yuwang You, Lu Cao, Bing Liang, Bo Tian, Jian Dong, Hong Lin
Summary: A bilayer coating with double-crosslinked hydrogels containing bone morphogenic protein was successfully established, showing great bio-compatibility and osteogenesis. The optimal ratios between GelMA and alginate were found to enhance the osteogenic transformation of hBMSCs.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biophysics
Yonglin Gao, Yating Kang, Tong Wang, Chengbo Li, Shengbiao Shen, Chenglei Qu, Shizhou Gong, Ping Liu, Lintong Yang, Jingmin Liu, Bing Han, Chunmei Li
Summary: Stem cell transplantation has shown promise in improving wound healing outcomes, but poor survival and retention of the transplanted cells have limited its application. A new three-dimensional culture system called AMS-Col gel was developed, which improved the survival and release of human umbilical cord mesenchymal stem cells (hUCMSC) for promoting wound healing. The use of AMS-Col gel resulted in improved collagen deposition, re-epithelialization, and wound healing.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Mohammed Zayed, Steve Adair, Madhu Dhar
Summary: The study showed that synovial fluid can maintain the proliferation and viability of MSCs and significantly affect chondrocyte differentiation. Stimulation of MSCs with synovial fluid or IFN-gamma can enhance chondrogenesis and anti-inflammatory activity. This suggests that the joint environment is suitable for chondrogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Binhong Teng, Siqi Zhang, Jijia Pan, Ziqian Zeng, Yang Chen, Yu Hei, Xiaoming Fu, Qian Li, Ming Ma, Yi Sui, Shicheng Wei
Summary: Hydrogel scaffolds functionalized with RGD and HAV peptides showed enhanced proliferation and adhesion of human mesenchymal stem cells (hMSCs), leading to greater chondrogenic differentiation compared to single-peptide groups. The controlled release of kartogenin (KGN) in the hydrogel facilitated cell chondrogenesis, while longer culture time resulted in cell condensation and significant matrix deposition in the RGD peptide groups. Through mimicking the cell microenvironment, the hydrogel system sequentially promoted the proliferation, adhesion, condensation, and chondrogenic differentiation of hMSCs during neo-chondrogenesis.
ACTA BIOMATERIALIA
(2021)
Article
Biotechnology & Applied Microbiology
A. Hagen, H. Lehmann, S. Aurich, N. Bauer, M. Melzer, J. Moellerberndt, V. Patane, C. L. Schnabel, J. Burk
Summary: This study aimed to establish a scalable protocol for equine platelet lysate (ePL) production and to test its effectiveness in equine MSC culture. Platelet concentrates prepared using the buffy coat method showed increased platelet and reduced white blood cell content, and were used successfully as a supplement in MSC culture. Further research into the functionality of equine MSC with ePL as a culture medium should be conducted.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Veterinary Sciences
Joao P. H. Pfeifer, Vitor H. Santos, Gustavo Rosa, Jaqueline B. Souza, Marcos Jun Watanabe, Carlos E. Fonseca-Alves, Elenice Deffune, Ana L. G. Alves
PESQUISA VETERINARIA BRASILEIRA
(2019)
Article
Chemistry, Multidisciplinary
Marli L. Moraes, Lais R. Lima, Josy C. Vicentini-Oliveira, Aparecida Vitoria G. de Souza, Osvaldo N. Oliveira, Elenice Deffune, Sidney J. L. Ribeiro
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
(2019)
Article
Veterinary Sciences
Vitor Hugo Dos Samos, Joao Pedro Hubbe Pfeifer, Jaqueline Brandao De Souza, Fernanda De Castro Stievani, Carlos Alberto Hussni, Marjorie De Assis Golim, Elenice Deffune, Ana Liz Garcia Alves
RESEARCH IN VETERINARY SCIENCE
(2019)
Article
Veterinary Sciences
Gabriela N. Dantas, Bianca P. Santarosa, Fernando J. Benesi, Vitor Hugo Santos, Roberto C. Goncalves
PESQUISA VETERINARIA BRASILEIRA
(2019)
Article
Veterinary Sciences
Gabriela N. Dantas, Bianca P. Santarosa, Vitor H. Santos, Fernando J. Benesi, Roberto C. Goncalves
PESQUISA VETERINARIA BRASILEIRA
(2019)
Article
Oncology
Thaila Quatrini Correa, Kate Cristina Blanco, Jennifer Machado Soares, Natalia Mayumi Inada, Cristina Kurachi, Marjorie de Assis Golim, Elenice Deffune, Vanderlei Salvador Bagnato
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2019)
Article
Veterinary Sciences
Gustavo Rosa, Andre Massahiro Teramoto Krieck, Enrico Padula, Joao Pedro Hubbe Pfeifer, Jaqueline Brandao de Souza, Mariana Rossi, Fernanda Stievani, Elenice Deffune, Regina Takahira, Ana Liz Garcia Alves
RESEARCH IN VETERINARY SCIENCE
(2020)
Article
Engineering, Biomedical
Mariana Correa Rossi, Daniel Leal Bayerlein, Jaqueline de Souza Brandao, Joao Pedro Hubbe Pfeifer, Gustavo dos Santos Rosa, William de Melo Silva, Luis Gallego Martinez, Margarida Juri Saeki, Ana Liz Garcia Alves
Summary: The study demonstrates that titanium scaffolds with stabilizing elements Nb and Sn exhibit higher wettability and better cellular migration performance when magnesium is added as spacer. Furthermore, the conditioned medium with TNS/M shows enhanced osteogenic differentiation potential for bone marrow-derived stem cells. These findings suggest that the powder metallurgy processed scaffold materials have promising potential for biomedical applications.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2021)
Article
Environmental Sciences
Helga Caputo Nunes, Samara Costa Tavares, Heloisa Vicente Garcia, Maira Smaniotto Cucielo, Sergio Alexandre Alcantara dos Santos, Mirian Carolini Esgoti Aal, Marjorie Assis de Golim, Luis Antonio Justulin Jr, Amanda Oliveira Ribeiro, Elenice Deffune, Wellerson Rodrigo Scarano, Flavia Karina Delella
Summary: Chemical contamination is an important factor to consider in cell therapy strategy design. This study found that BPA and TCDD can significantly reduce cell viability and affect adipogenic differentiation and DNA damage.
ENVIRONMENTAL TOXICOLOGY
(2022)
Article
Materials Science, Paper & Wood
Amanda Maria Claro, Nayara Cavichiolli Do Amaral, Vitoria Maria Medalha Colturato, Nadia Andrade Aleixo, Robert Paiva, Sandra Andrea Cruz, Gustavo Claro Monteiro, Gustavo Senra Goncalves De Carvalho, Flavia Aparecida Resende Nogueira, Elenice Deffune, Monica Rosas da Costa Iemma, Hernane da Silva Barud
Summary: This study functionalized bacterial cellulose (BC) membranes to improve cell adhesion. The results showed that the MPTMS modification on BC surface was efficient regardless of the drying process. Cell adhesion to the BC surface depends on both the functional group in the matrix and the surface wettability.
Article
Surgery
Geruza Rezende Paiva, Fausto Viterbo, Elenice Deffune, Maria Aparecida Domingues Custodio
ACTA CIRURGICA BRASILEIRA
(2020)
Article
Medicine, General & Internal
Lais Priscila De Santis, Patricia Carvalho Garcia, Valeria Nogueira Dias Paes Secco, Rosana Rossi Ferreira, Elenice Deffune
EINSTEIN-SAO PAULO
(2019)
