Article
Cell Biology
Qi Wen Teo, Yiquan Wang, Huibin Lv, Timothy J. C. Tan, Ruipeng Lei, Kevin J. Mao, Nicholas C. Wu
Summary: This study reveals the importance of light-chain and CDR H3 sequences for the HA stem specificity of IGHV1-69 antibodies, providing molecular insights into broadly neutralizing antibody responses to influenza virus and important implications for universal influenza vaccine development.
Article
Biochemistry & Molecular Biology
Zuzana Rosenbergova, Kristina Kantorova, Martin Simkovic, Albert Breier, Martin Rebros
Summary: The study successfully upscaled the production of Myrosinase to a 3 L laboratory fermenter, optimizing fermentation conditions resulted in a threefold increase in Myrosinase productivity and 1.5-fold increase in dry cell weight.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Food Science & Technology
Muhammad Ijaz Ahmad, Shahzad Farooq, Yasmin Alhamoud, Chunbao Li, Hui Zhang
Summary: This paper focuses on the structure and production stages of LegH preparation as well as its safety and food applications. Although there is still limited research on LegH, it has great potential in developing meat analogs with enhanced flavor and color.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Jungsoon Lee, Zhuyun Liu, Wen-Hsiang Chen, Junfei Wei, Rakhi Kundu, Rakesh Adhikari, Joanne Altieri Rivera, Portia M. Gillespie, Ulrich Strych, Bin Zhan, Peter J. Hotez, Maria Elena Bottazzi
Summary: The study presented the development and scale-up optimization of production and purification process for RBD219-N1C1 protein antigen against SARS-CoV-2, with the selected process yielding high purity and recovery rate of the product. Different chromatographic purification methods were compared, and the best process was chosen based on analytical studies. An optimized production process was proposed, which can generate high-quality RBD219-N1C1 protein antigen at a low cost and scalable level.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Neil C. Dalvie, Sergio A. Rodriguez-Aponte, Brittany L. Hartwell, Lisa H. Tostanoski, Andrew M. Biedermann, Laura E. Crowell, Kawaljit Kaur, Ozan S. Kumru, Lauren Carter, Jingyou Yu, Aiquan Chang, Katherine McMahan, Thomas Courant, Celia Lebas, Ashley A. Lemnios, Kristen A. Rodrigues, Murillo Silva, Ryan S. Johnston, Christopher A. Naranjo, Mary Kate Tracey, Joseph R. Brady, Charles A. Whittaker, Dongsoo Yun, Natalie Brunette, Jing Yang Wang, Carl Walkey, Brooke Fiala, Swagata Kar, Maciel Porto, Megan Lok, Hanne Andersen, Mark G. Lewis, Kerry R. Love, Danielle L. Camp, Judith Maxwell Silverman, Harry Kleanthous, Sangeeta B. Joshi, David B. Volkin, Patrice M. Dubois, Nicolas Collin, Neil P. King, Dan H. Barouch, Darrell J. Irvine, J. Christopher Love
Summary: Subunit vaccines based on recombinant proteins are crucial tools for controlling COVID-19 due to their low manufacturing cost, scalability, and proven safety and efficacy. Engineered RBD variants exhibit high manufacturability and immunogenicity, showing enhanced immune responses after a single dose and cross-reactivity to new variants of concern.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Immunology
Christopher L. D. McMillan, Paul R. Young, Daniel Watterson, Keith J. Chappell
Summary: Current influenza virus vaccines mainly induce antibodies against the highly variable head domain of the hemagglutinin protein, but these antibodies are often strain-specific, resulting in limited cross-protection. Therefore, the annual update of vaccine formulations to counteract the challenge of influenza virus evolution is crucial.
Article
Biotechnology & Applied Microbiology
Korbinian Sinzinger, Sebastian Bieringer, Doris Schieder, Herbert Riepl, Volker Sieber
Summary: This study explores the feasibility of using a cyanobacterial strain as a potential feedstock for a biorefinery concept. The results show that the cyanobacterial biomass has a high protein content and the potential to produce multiple high-value products. The fermentation experiment using the hydrolysate suggests that the cyanobacterial biomass can be used as an effective medium supplement for enhancing enzyme expression. These findings provide a basis for further development of biorefinery concepts.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Biotechnology & Applied Microbiology
Neil C. Dalvie, Joseph R. Brady, Laura E. Crowell, Mary Kate Tracey, Andrew M. Biedermann, Kawaljit Kaur, John M. Hickey, D. Lee Kristensen, Alexandra D. Bonnyman, Sergio A. Rodriguez-Aponte, Charles A. Whittaker, Marina Bok, Celina Vega, Tarit K. Mukhopadhyay, Sangeeta B. Joshi, David B. Volkin, Viviana Parreno, Kerry R. Love, J. Christopher Love
Summary: This study demonstrates a holistic approach for molecular design of recombinant protein antigens, improving manufacturability and reducing product variants while maintaining original antigenicity and immunogenicity. The engineered NRRV antigens enabled simultaneous production and purification of all three serotypes in a single process, showcasing the potential to lower cost and volumetric requirements in manufacturing vaccines based on recombinant protein subunits.
MICROBIAL CELL FACTORIES
(2021)
Article
Immunology
Jessica A. Flynn, Teresa Weber, Pedro J. Cejas, Kara S. Cox, Sinoeun Touch, Lauren A. Austin, Yangsi Ou, Michael P. Citron, Bin Luo, Marian E. Gindy, Kapil Bahl, Giuseppe Ciaramella, Amy S. Espeseth, Lan Zhang
Summary: mRNA vaccines encoding conserved influenza antigens, such as HA stem and NP, induce broadly reactive humoral and cell-mediated immune responses in mice and nonhuman primates, providing protection against homologous and heterologous influenza infection.
Article
Virology
Lee Sherry, Keith Grehan, Jessica J. Swanson, Mohammad W. Bahar, Claudine Porta, Elizabeth E. Fry, David Stuart, David J. Rowlands, Nicola J. Stonehouse
Summary: The production of wild poliovirus (PV) virus-like particles (VLPs) with native conformation using Pichia pastoris shows potential as a next-generation vaccine candidate for polio. These VLPs provide improved antigen ratio and thermostability compared to wild PV-3, highlighting their potential for polio eradication.
Article
Biochemistry & Molecular Biology
Katherine Houser, Grace L. Chen, Cristina Carter, Michelle C. Crank, Thuy A. Nguyen, Maria Claudia Burgos Florez, Nina M. Berkowitz, Floreliz Mendoza, Cynthia Starr Hendel, Ingelise J. Gordon, Emily E. Coates, Sandra Vazquez, Judy Stein, Christopher L. Case, Heather Lawlor, Kevin Carlton, Martin R. Gaudinski, Larisa Strom, Amelia R. Hofstetter, C. Jason Liang, Sandeep Narpala, Christian Hatcher, Rebecca A. Gillespie, Adrian Creanga, Masaru Kanekiyo, Julie E. Raab, Sarah F. Andrews, Yi Zhang, Eun Sung Yang, Lingshu Wang, Kwanyee Leung, Wing-Pui Kong, Alec W. Freyn, Raffael Nachbagauer, Peter Palese, Robert T. Bailer, Adrian B. McDermott, Richard A. Koup, Jason G. Gall, Frank Arnold, John R. Mascola, Barney S. Graham, Julie E. Ledgerwood
Summary: This study evaluated the clinical trial results of a novel ferritin nanoparticle influenza vaccine platform called H2HA-Ferritin. The trial showed that the vaccine is safe and well tolerated, and it can elicit neutralizing antibody responses in both individuals who have not been exposed to the H2 subtype and those who have been exposed. Additionally, the vaccine also induced immune responses against multiple influenza subtypes. This vaccine technology has the potential for applications in pandemic preparedness and universal influenza vaccine development.
Article
Biotechnology & Applied Microbiology
Carly M. Bliss, Alec W. Freyn, Tom G. Caniels, Victor H. Leyva-Grado, Raffael Nachbagauer, Weina Sun, Gene S. Tan, Virginia L. Gillespie, Meagan McMahon, Florian Krammer, Adrian V. S. Hill, Peter Palese, Lynda Coughlan
Summary: Conventional influenza vaccines fail to provide broad protection against diverse influenza A viruses. However, this study presents a non-replicating adenoviral vaccine that can elicit broad and cross-reactive protection against multiple strains of influenza A in mice.
Review
Immunology
Rina Fajri Nuwarda, Abdulsalam Abdullah Alharbi, Veysel Kayser
Summary: Influenza is a major public health concern, and vaccines are an effective method for prevention and control. New vaccine platforms and advancements in vaccine manufacturing processes are being explored to improve effectiveness and stability.
Article
Agricultural Engineering
Hongzhen Luo, Tairan Zhou, Jin Cao, Lei Gao, Shijie Wang, Zheng Gui, Yongjiang Shi, Fang Xie, Rongling Yang
Summary: Glycerol organosolv pretreatment (GOP) is an efficient method for deconstructing lignocellulose to produce fermentable sugars. In this study, the liquid fraction containing glycerol after GOP was used for recycled pretreatment of corn stover (CS) for four cycles. The enzymatic yield of glucose after recycled pretreatment was significantly increased compared to untreated CS. The residual glycerol was also utilized as a carbon source for the cultivation of Pichia pastoris, which produced a high titer of human lysozyme. Additionally, the pretreated CS was mixed with cassava and used as a fermentable substrate for butanol production by Clostridium acetobutylicum ATCC 824, resulting in a high butanol production yield. The integration of glycerol utilization and butanol production provided an efficient strategy for biorefinery.
BIORESOURCE TECHNOLOGY
(2023)
Article
Engineering, Chemical
Yanan Sheng, Jiangxue Wei, Zhengjun Li, Zhiguo Su, Guanghui Ma, Songping Zhang
Summary: In this study, ferritin nanoparticles were used as a carrier for influenza vaccine by chemically conjugating M2e and HA antigens on the outer surface. The dual-antigen vaccine candidate showed high levels of antigen-specific antibodies in immunized mice and provided effective protection against both homologous and heterologous influenza virus strains.
Article
Chemistry, Medicinal
Peng-Cheng Wang, Din-Chi Chiu, Jia-Tsrong Jan, Wen-I Huang, Yin-Chen Tseng, Ting-Ting Li, Ting-Jen Cheng, Keng-Chang Tsai, Jim-Min Fang
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2018)
Article
Chemistry, Medicinal
Jui-Yin Yu, Hsiu-Jung Cheng, Huei-Ru Wu, Wei-Shen Wu, Jui-Wen Lu, Ting-Jen Cheng, Ying-Ta Wu, Jim-Min Fang
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2018)
Article
Chemistry, Medicinal
Bei-Tao Hong, Yih-Shyun E. Cheng, Ting-Jen Cheng, Jim-Min Fang
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Jing Li, Damao Wang, Xiaohui Xing, Ting-Jen Rachel Cheng, Pi-Hui Liang, Vincent Bulone, Jeong Hill Park, Yves S. Y. Hsieh
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2019)
Article
Chemistry, Medicinal
Meng-Ruo Huang, Yuan-Ling Hsu, Tzu-Chen Lin, Ting-Jen Cheng, Ling-Wei Li, Yu-Wei Tseng, Yu-Shu Chou, Jyung-Hurng Liu, Szu-Hua Pan, Jim -Min Fang, Chi -Huey Wong
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2019)
Article
Chemistry, Medicinal
Ming-Shiu Lin, Tse-Ming Hong, Ting-Hung Chou, Shuenn-Chen Yang, Wei-Chia Chung, Chia-Wei Weng, Mei-Ling Tsai, Ting-Jen Rachel Cheng, Jeremy J. W. Chen, Te-Chang Lee, Chi-Huey Wong, Rong-Jie Chein, Pan-Chyr Yang
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2019)
Article
Evolutionary Biology
Woei-Fuh Wang, Mei-Yeh Jade Lu, Ting-Jen Rachel Cheng, Yi-Ching Tang, Yu-Chuan Teng, Teh-Yang Hwa, Yi-Hua Chen, Meng-Yun Li, Mei-Hua Wu, Pei-Chun Chuang, Ruwen Jou, Chi-Huey Wong, Wen-Hsiung Li
GENOME BIOLOGY AND EVOLUTION
(2020)
Article
Chemistry, Medicinal
Jennifer L. Woodring, Shao-Hung Lu, Larissa Krasnova, Shih-Chi Wang, Jhih-Bin Chen, Chiu-Chun Chou, Yi-Chou Huang, Ting-Jen Rachel Cheng, Ying-Ta Wu, Yu-Hou Chen, Jim-Min Fang, Ming-Daw Tsai, Chi-Huey Wong
JOURNAL OF MEDICINAL CHEMISTRY
(2020)
Article
Chemistry, Medicinal
Chia-Yi Lin, Hsin-Yi Wu, Yuan-Ling Hsu, Ting-Jen Rachel Cheng, Jyung-Hurng Liu, Rou-Jie Huang, Tzu-Hung Hsiao, Chia-Jen Wang, Pei-Fang Hung, Albert Lan, Szu-Hua Pan, Rong-Jie Chein, Chi-Huey Wong, Pan-Chyr Yang
JOURNAL OF MEDICINAL CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Li-Ting Chiu, Narayana Murthy Sabbavarapu, Wei-Chen Lin, Chiao-Yuan Fan, Chih-Chung Wu, Ting-Jen Rachel Cheng, Chi-Huey Wong, Shang-Cheng Hung
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Multidisciplinary Sciences
Hsin-Yu Liao, Shih-Chi Wang, Yi-An Ko, Kuo- Lin, Che Ma, Ting-Jen Rachel Cheng, Chi-Huey Wong
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Multidisciplinary Sciences
Jia-Tsrong Jan, Ting-Jen Rachel Cheng, Yu-Pu Juang, Hsiu-Hua Ma, Ying-Ta Wu, Wen-Bin Yang, Cheng-Wei Cheng, Xiaorui Chen, Ting-Hung Chou, Jiun-Jie Shie, Wei-Chieh Cheng, Rong-Jie Chein, Shi-Shan Mao, Pi-Hui Liang, Che Ma, Shang-Cheng Hung, Chi-Huey Wong
Summary: The COVID-19 pandemic has resulted in over 50 million confirmed cases and more than 1 million deaths globally as of November 2020. Currently, there are no effective antivirals approved by the FDA, posing a major challenge in vaccine development. Alternative treatments are urgently needed.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Jing Li, Shih-Yi Hsiung, Mu-Rong Kao, Xiaohui Xing, Shu-Chieh Chang, Damao Wang, Pei-Yun Hsieh, Pi -Hui Liang, Zaibiao Zhu, Ting -Jen Rachel Cheng, Jiun-Jie Shie, Jing-Ping Liou, D. Wade Abbott, Sung Won Kwon, Yves S. Y. Hsieh
Summary: This study investigated the cell wall structures of Polygonatum odoratum in different plant parts. It was found that the rhizome contains a higher proportion of heteromannans compared to the leaf and stem. The pectic polysaccharides in the rhizome were also structurally more diverse. The 2-linked Araf was specific to the rhizome, indicating a more complex cell wall structure. Additionally, a high proportion of HMs was found in the rhizome cell walls, which induced a specific immune response in mice macrophage cells.
CARBOHYDRATE RESEARCH
(2022)
Article
Cell Biology
Ming-Han Chen, Ming-Ting Huang, Wen-Kuang Yu, Shinn-Shing Lee, Jia-Horng Wang, Ting-Jen R. Cheng, Michael R. Bowman, Shie-Liang Hsieh
JOURNAL OF BIOMEDICAL SCIENCE
(2019)
Article
Virology
Naiqing Xu, Xinen Tang, Xin Wang, Miao Cai, Xiaowen Liu, Xiaolong Lu, Shunlin Hu, Min Gu, Jiao Hu, Ruyi Gao, Kaituo Liu, Yu Chen, Xiufan Liu, Xiaoquan Wang
Summary: This study found that the H9N2 subtype avian influenza virus has a high airborne transmissibility, while the H7N9 virus does not. The Hemagglutinin protein of the H9N2 virus was found to play a key role in replication, stability, and airborne transmission.
Article
Virology
Samar S. Ewies, Sabry M. Tamam, Ahmed S. Abdel-Moneim, Sherin R. Rouby
Summary: Contagious ecthyma (CE) is a highly contagious viral disease of sheep and goats worldwide. The study provided a clinical description of CE and screened for genetic variation in the B2L gene. Infected sheep exhibited anorexia and oral lesions, while inoculated chicken embryos showed pock lesions. The B2L gene was successfully amplified and found to be highly conserved.
Article
Virology
Yigal Farnoushi, Dan Heller, Avishai Lublin
Summary: In recent years, new variants of avian reovirus (ARV) have caused a variety of symptoms in chickens worldwide, including viral arthritis/tenosynovitis. This study analyzed emerging ARV variants in Israel and found significant genetic diversity. Most ARV isolates in Israel belonged to genotypic cluster 5 (GC5). The study suggests that Israel has not experienced the emergence of new ARV variants since the introduction of the live vaccine (ISR-7585), but ongoing monitoring is needed due to the continuous emergence of ARV variants.
Article
Virology
Shigeru Tajima, Michiyo Kataoka, Yuki Takamatsu, Hideki Ebihara, Chang-Kweng Lim
Summary: Yokose virus (YOKV), a bat-associated flavivirus, was found to replicate at a slower rate in mosquito cells compared to other mosquito-borne flaviviruses. Specific nucleotide mutations in the virus were identified to enhance its proliferation ability in mosquito cells.
Article
Virology
Alejandra Borjabad, Baojun Dong, Wei Chao, David J. Volsky, Mary Jane Potash
Summary: This study investigated HIV brain disease using a mouse model, and found that poly I:C can reverse associated cognitive impairment and reduce virus burden. The results also revealed transcriptional changes related to neuronal function and innate immune responses.
Article
Virology
Ching-Hung Lin, Feng-Cheng Hsieh, Meilin Wang, Chieh Hsu, Hsuan-Wei Hsu, Chun-Chun Yang, Cheng-Yao Yang, Hung-Yi Wu
Summary: This study demonstrates that the synthesis of coronavirus subgenomic mRNA is not solely determined by the sequence homology between the leader TRS and TRS-B, but also by the disassociation of the coronavirus polymerase from the viral genome. This finding provides a new insight into the transcription mechanism of coronaviruses.
Article
Virology
Nicholas S. Kron, Benjamin W. Neuman, Sathish Kumar, Patricia L. Blackwelder, Dayana Vidal, Delphina Z. Walker-Phelan, Patrick D. I. Gibbs, Lynne A. Fieber, Michael C. Schmale
Summary: Two recent studies documented the genome of a novel virus in marine animals, finding that the virus is widespread in apparently healthy animals but not highly expressed in neurons. The studies also identified viral replication factories and high levels of defective genomes in chronically infected animals.
Article
Virology
Andrew M. Ramey, Laura C. Scott, Christina A. Ahlstrom, Evan J. Buck, Alison R. Williams, Mia Kim Torchetti, David E. Stallknecht, Rebecca L. Poulson
Summary: We successfully detected and characterized highly pathogenic avian influenza viruses in hunter-harvested wild waterfowl samples from western Alaska. Genomic analysis revealed three independent viral introductions into Alaska. Our findings demonstrate the utility and potential limitations of using molecular processing approaches directly on original swab samples for viral research and monitoring.
Article
Virology
Ting Gong, Dongdong Wu, Yongzhi Feng, Xing Liu, Qi Gao, Xiaoyu Zheng, Zebu Song, Heng Wang, Guihong Zhang, Lang Gong
Summary: This study discovered that quercetin can inhibit PEDV replication both in vivo and in vitro, and alleviate the clinical symptoms and intestinal injury caused by the virus. This provides a new direction for the development of PED antiviral drugs.
Article
Virology
Min Zhu, Hao Zeng, Jianqiao He, Yaohui Zhu, Pingping Wang, Jianing Guo, Jinfan Guo, Huabo Zhou, Yifeng Qin, Kang Ouyang, Zuzhang Wei, Weijian Huang, Ying Chen
Summary: The reassortment between avian H9N2 and Eurasian avian-like (EA) H1N1 viruses may have potentially changed from avian-to-mammals adaptation. This study found that the introduction of EA H1N1 internal genes into H9N2 virus restored the replication capability and resulted in extreme virulence in some cases. This raises new concerns for public health due to the possible coexistence of H9N2 and EA H1N1 viruses in dogs.
