Article
Plant Sciences
Raheleh Karimi-Ashtiyani, Veit Schubert, Andreas Houben
Summary: Our study shows that only the rye-derived centromere part in the wheat-rye 1BL/1RS translocation chromosome incorporates wheat CENH3, supporting the idea that one centromere part undergoes inactivation in a translocated chromosome to maintain chromosome stability.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Forestry
Zhibo Wang, Zihang He, Xin Xu, Xinxin Shi, Xiaoyu Ji, Yucheng Wang
Summary: The study identified multiple salt tolerance genes in Tamarix hispida through yeast expression system and high-throughput sequencing, demonstrating their reliability in enhancing plant salt tolerance. The research also observed higher expression levels of identified genes in roots under salt stress, with a significant proportion belonging to 'response to stimulus' category. The findings suggest that processes such as protein translation, osmotic adjustment, antioxidant defense, and maintenance of cellular functions play crucial roles in salt tolerance mechanisms.
Article
Microbiology
Zhengfeng Wang, Jing Liu, James F. F. White, Chunjie Li
Summary: This study suggests that Epichloe bromicola derived from wild barley has the potential to increase salt tolerance in cultivated barley. The inoculation of E. bromicola can enhance the growth and salt tolerance of barley, with effects varying under different salt stress conditions. These findings provide new insights into the mechanism of barley-fungal endophyte association and raise interesting questions about the role of E. bromicola in fungus-enhanced tolerance to salt stress in this symbiosis.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Huang Yiwen, Dai Xuran, Liu Hongwei, Yu Shuo, Mai Chunyan, Yu Liqiang, Yu Guangjun, Yang Li, Zhou Yang, Li Hongjie, Zhang Hongjun
Summary: The study evaluated the effects of allelic variation and haplotypes in genes conferring PHS resistance in winter wheat cultivars by testing PHS resistance in 326 commercial cultivars over three growing seasons. The results identified useful alleles and haplotypes for improving PHS resistance and highlighted the potential of introgressing specific alleles to enhance resistance. This research provides valuable information for breeding programs aiming at enhancing PHS resistance in winter wheat cultivars.
Article
Agronomy
Iwona Jaskulska, Dariusz Jaskulski
Summary: The study showed that the strip-till one-pass technology can improve yields and quality in winter wheat and spring barley cultivation compared to traditional tillage methods, especially performing better under conditions of insufficient rainfall.
Article
Plant Sciences
Wenqiang Wang, Yang Yang, Yanmei Deng, Zhigang Wang, Yuchao Yuan, Shenlin Yang, Juan Qi, Jiajie Wu, Daolin Fu, Wei Wang, Qunqun Hao
Summary: The study found that barley transgenic lines overexpressing ICS exhibit enhanced salt stress tolerance, attributed to improved Na+/K+ ratio, Na+ sequestration, and antioxidative competence under salt stress.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Review
Agriculture, Multidisciplinary
Zihan Li, Fan Zhong, Jianrong Guo, Zhuo Chen, Jie Song, Yi Zhang
Summary: Salinity is a major abiotic stress that affects crop yield and food supply in saline soil areas. Wheat, being the main crop in these areas, has developed various strategies to enhance salt tolerance through conventional breeding approaches and new techniques such as gene identification, gene stacking, and utilization of wild relatives.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Environmental Sciences
Katharina Harfenmeister, Sibylle Itzerott, Cornelia Weltzien, Daniel Spengler
Summary: Monitoring the phenological development of winter wheat and winter barley using remote sensing features such as backscatter, polarimetric parameters, and NDVI shows sensitivity to specific stages. The approach demonstrates transferability across test sites and years, with differences mainly attributed to meteorological variations.
Article
Plant Sciences
Zhengyuan Xu, Qiufang Shen, Guoping Zhang
Summary: This study compared the growth and physiological traits of sea barley, wheat, and barley under waterlogging stress and found that sea barley exhibited the strongest waterlogging tolerance. Sea barley showed constitutive and induced aerenchyma, while wheat and barley did not.
PLANT GROWTH REGULATION
(2022)
Article
Genetics & Heredity
Paula Silva, Byron Evers, Alexandria Kieffaber, Xu Wang, Richard Brown, Liangliang Gao, Allan Fritz, Jared Crain, Jesse Poland
Summary: This study evaluated the use of high-throughput phenotyping to improve the assessment of barley yellow dwarf, identified genomic regions associated with resistance, and assessed the ability of genomic selection models to predict resistance. The results showed that insecticide treatment reduced the severity of barley yellow dwarf, and identified a translocated segment carrying the resistance gene Bdv2 through association mapping. A potentially novel genomic region for resistance was also mapped. The study demonstrated the potential of high-throughput phenotyping and genomic selection models for improving the assessment of barley yellow dwarf and identifying new sources of resistance.
G3-GENES GENOMES GENETICS
(2022)
Article
Plant Sciences
Xiaoyan Quan, Xiaoli Liang, Hongmei Li, Chunjuan Xie, Wenxing He, Yuxiang Qin
Summary: Salinity is a major constraint to wheat production globally. Research using a diverse range of wheat genotypes identified 12 salt-tolerant genotypes, which showed increased accumulation of osmotic substances, efficient use of soluble sugars, lower Na+/K+ ratio, and a more efficient antioxidative system under salt stress. These genotypes were able to maintain stable osmotic potential, ion balance, and provide more resources for root growth, suggesting potential for developing new salt-tolerant wheat cultivars and understanding genetic mechanisms of salt tolerance in wheat.
Article
Plant Sciences
Ying Jiang, Haiwen Zhang, Yang Li, Congcong Chang, Yunxiao Wang, Hao Feng, Ruifen Li
Summary: This study identified a novel salt stress-responsive mechanism in the halophytic plant Hordeum brevisubulatum, shedding light on the adaptive mechanisms of salt-tolerant plants.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Magdalena Wojcik-Jagla, Agata Daszkowska-Golec, Anna Fiust, Przemyslaw Kopec, Marcin Rapacz
Summary: The study on the mechanism of winter barley de-acclimation identified a lower number of transcripts changing their accumulation levels in opposite directions during acclimation and de-acclimation, with more genes showing expression changes in de-acclimation-susceptible accessions, especially related to stress response genes. Mid-winter de-acclimation seems to be perceived as an opportunity for regeneration, but remaining in the cold-acclimated state is competitive. The response to mid-winter de-acclimation is more extensive in de-acclimation-susceptible cultivars, suggesting reduced response to rising temperatures is crucial for de-acclimation tolerance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Multidisciplinary
Fuyong Wu, Wanqing Luo, Jiao Li, Wenjing Xing, Lihui Lyu, Jing Yang, Ruifang Liu, Zhaoyong Shi
Summary: Based on a pot trial, this study found that arbuscular mycorrhizal fungi (AMF) have an influence on grain selenium (Se) concentration in winter wheat. Rhizophagus intraradices (Ri) significantly enhanced Se accumulation under selenite treatment, and inoculation of AMF increased Se content under selenite and selenate treatments.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Plant Sciences
Yingying Lu, Wieland Fricke
Summary: The aim of this study was to quantify the contribution of apoplastic bypass flow to water and salt uptake across the root cylinder of wheat and barley during day and night. The results showed that the contribution of bypass flow to root water uptake increased in response to salt stress and during the night, reaching up to 4.4%. Bypass flow also made up 2%-12% of the net delivery of Na+ and Cl- ions to the shoot.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Agronomy
Kitti Szoke-Pazsi, Edina Turkosi, Eva Szakacs
Summary: The study aimed to create a detailed karyotype of the perennial Secale cereanum cultivar 'Kriszta', particularly focusing on the long arms, in order to identify translocated chromosomes. Results revealed that the chromosome complement of 'Kriszta' is not a simple combination of parental rye species, and that a specific pair-wise combination of DNA sequences can identify its long arms. Future usability of the identified wheat-'Kriszta' translocated chromosomes is also discussed.
CEREAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Laszlo Ivanizs, Ilaria Marcotuli, Marianna Rakszegi, Balazs Kalapos, Kitti Szoke-Pazsi, Andras Farkas, Edina Turkosi, Eszter Gaal, Klaudia Kruppa, Peter Kovacs, Eva Darko, Eva Szakacs, Mahmoud Said, Petr Capal, Jaroslav Dolezel, Agata Gadaleta, Istvan Molnar
Summary: This study examined the genetic variability in grain beta-glucan, pentosan, and protein content in Aegilops biuncialis and identified 34 QTLs associated with these traits. Mapping the markers to chromosome assemblies identified the role of genes on certain chromosomes in the formation of grain beta-glucan content. The QTLs and genes identified in this study are potential targets for improving the health-promoting properties of wheat-derived foods.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jana Zwyrtkova, Nicolas Blavet, Alzbeta Dolezalova, Petr Capal, Mahmoud Said, Istvan Molnar, Jan Vrana, Jaroslav Dolezel, Eva Hribova
Summary: In this study, the complex genome of Crested wheatgrass was analyzed using flow sorting and DNA sequencing. Genes related to plant disease resistance and stress tolerance were identified, and a new DNA repeat element was discovered. Additionally, SSR markers were identified for future breeding purposes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Guotai Yu, Oadi Matny, Nicolas Champouret, Burkhard Steuernagel, Matthew J. Moscou, Inmaculada Hernandez-Pinzon, Phon Green, Sadiye Hayta, Mark Smedley, Wendy Harwood, Ngonidzashe Kangara, Yajuan Yue, Catherine Gardener, Mark J. Banfield, Pablo D. Olivera, Cole Welchin, Jamie Simmons, Eitan Millet, Anna Minz-Dub, Moshe Ronen, Raz Avni, Amir Sharon, Mehran Patpour, Annemarie F. Justesen, Murukarthick Jayakodi, Axel Himmelbach, Nils Stein, Shuangye Wu, Jesse Poland, Jennifer Ens, Curtis Pozniak, Miroslava Karafiatova, Istvan Molnar, Jaroslav Dolezel, Eric R. Ward, T. Lynne Reuber, Jonathan D. G. Jones, Martin Mascher, Brian J. Steffenson, Brande B. H. Wulff
Summary: In this study, the authors assembled the genome of the wild diploid wheat relative Aegilops sharonensis and successfully cloned the stem rust resistance gene Sr62. The results show that Sr62 can be transferred to common wheat and provides high levels of resistance. This has significant implications for improving the disease resistance of wheat.
NATURE COMMUNICATIONS
(2022)
Article
Plant Sciences
Edina Tuerkoesi, Laszlo Ivanizs, Andras Farkas, Eszter Gaal, Klaudia Kruppa, Peter Kovacs, Eva Szakacs, Kitti Szoke-Pazsi, Mahmoud Said, Petr Capal, Simon Griffiths, Jaroslav Dolezel, Istvan Molnar
Summary: To improve wheat using genetic diversity in wild relatives, recombination between wheat and alien chromosomes is necessary. However, this recombination is suppressed by the Ph1 gene on wheat chromosome 5B. The ph1b mutation has been used to induce recombination in wheat x alien hybrids, but the original ph1b mutation in Chinese Spring has poor agronomic performance. To overcome this, alien introgression lines were backcrossed with adapted wheat genotypes. In this study, the ph1b mutation was transferred from Chinese Spring mutants to the winter wheat line Mv9kr1, resulting in improved plant and spike morphology. The Mv9kr1ph1b genotype allowed for wheat-alien chromosome pairing in hybrids, making it a valuable resource for alien introgression breeding in hexaploid wheat.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Genetics & Heredity
Solmaz Najafi, Mehmet Ulker, Erol Oral, Ruveyde Tuncturk, Murat Tuncturk, R. Z. Sayyed, Kahkashan Perveen, Peter Poczai, Andras Cseh
Summary: This study evaluated cytogenetic analyses in different Aegilops species using nuclear DNA quantification. The results showed significant differences in DNA content among diploid and tetraploid species in Turkey and Iran, but not in hexaploid species. Intraspecific genome size variation was low, while interspecific variation was significant. The flow cytometry technique allowed for easy and accurate differentiation of Aegilops species.
Article
Genetics & Heredity
Diana Makai, Andras Cseh, Adel Sepsi, Szabolcs Makai
Summary: This study proposes a method to represent Hi-C datasets using pseudo-structures in three-dimensional space. Although these structures are not directly observed, they show overall similarities to known genome structures and have potential for further expansion.
Article
Genetics & Heredity
Guotai Yu, Oadi Matny, Spyridon Gourdoupis, Naganand Rayapuram, Fatimah R. Aljedaani, Yan L. Wang, Thorsten Nuernberger, Ryan Johnson, Emma E. Crean, Isabel M. -L. Saur, Catherine Gardener, Yajuan Yue, Ngonidzashe Kangara, Burkhard Steuernagel, Sadiye Hayta, Mark Smedley, Wendy Harwood, Mehran Patpour, Shuangye Wu, Jesse Poland, Jonathan D. G. Jones, T. Lynne Reuber, Moshe Ronen, Amir Sharon, Matthew N. Rouse, Steven Xu, Katerina Holusova, Jan Bartos, Istvan Molnar, Miroslava Karafiatova, Heribert Hirt, Ikram Blilou, Lukasz Jaremko, Jaroslav Dolezel, Brian J. Steffenson, Brande B. H. Wulff
Summary: To protect bread wheat from pests and diseases, breeders have introduced over 200 resistance genes, nearly doubling the number of designated resistance genes in its gene pool. One of these genes, Sr43, which was derived from the wild grass Thinopyrum elongatum, encodes a protein kinase fused to two unknown function domains. Transgenic expression of Sr43 in wheat provides high levels of resistance against various isolates of stem rust, indicating its potential in resistance breeding and engineering.
Article
Genetics & Heredity
Yajun Wang, Michael Abrouk, Spyridon Gourdoupis, Dal-Hoe Koo, Miroslava Karafiatova, Istvan Molnar, Katerina Holusova, Jaroslav Dolezel, Naveenkumar Athiyannan, Emile Cavalet-Giorsa, Lukasz Jaremko, Jesse Poland, Simon G. Krattinger
Summary: To enrich crop germplasm with disease-resistance genes, we successfully cloned the leaf rust resistance gene Lr9 through mutagenesis and transcriptome sequencing, and found that Lr9 encodes an unusual tandem kinase fusion protein. By utilizing long-read sequencing, we identified the Lr9 translocation of approximately 28.4-Mb and the translocation breakpoint in a wheat Lr9 introgression line and the putative Ae. umbellulata Lr9 donor. We also discovered a homologous gene Lr58 with the same coding sequence as Lr9, which was reportedly introgressed from Aegilops triuncialis, indicating a common translocation event. Our work sheds light on the emerging role of kinase fusion proteins in wheat disease resistance, expanding the repertoire of disease-resistance genes for breeding.
Article
Plant Sciences
Adam Horvath, Tibor Kiss, Zita Berki, Adam D. Horvath, Krisztina Balla, Andras Cseh, Otto Veisz, Ildiko Karsai
Summary: The dynamics of plant development are important for both ecological adaptation and achieving genetically determined yield potentials in different environments. Global climate change makes it urgent to understand the genetic factors influencing plant development, as it can disrupt locally adapted developmental patterns. This study used genetic analysis and field experiments to identify genetic determinants of plant development and their impact on yield formation. It found that the photoperiod response gene PPD-D1 played a major role, explaining a significant portion of phenotypic variation. Additionally, several minor developmental loci were identified, each explaining a small portion of the variance but collectively contributing to a substantial amount. The study also found that the combination of PPD-D1 insensitivity or sensitivity with different alleles in minor developmental loci resulted in distinct plant developmental patterns and affected yield-related traits. These findings have implications for ecological adaptation.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemical Research Methods
Diana Makai, Edit Mihok, David Polgari, Andras Cseh, Andrea Lenyko-Thegze, Adel Sepsi, Laszlo Sagi
Summary: A quick and reliable method was developed for preparing plant cell nuclei suitable for combined immunolabelling and fluorescence in situ hybridisation methods. The method involves mechanical removal of the cell wall and cytoplasm, and in-solution cell handling throughout the fixation and preparation steps. It is compatible with immunolabelling, FISH, and GISH as well as their simultaneous combinations.
Article
Cell Biology
Jaroslav Dolezel, Petr Urbis, Mahmoud Said, Sergio Lucretti, Istvan Molnar
Summary: Flow cytogenetics is a high-throughput technique used for classifying and studying large populations of chromosomes. The most common application is the isolation of different chromosomes, which allows for targeted, simplified, and more economical genomic analyses. The uses of flow cytogenetics have expanded with advances in molecular biology and genomics techniques, including the development of DNA markers, construction of chromosome-specific DNA libraries, physical mapping, draft genome sequencing, and gene cloning.
Article
Agronomy
Caiyun Liu, Wei Guo, Yang Wang, Bisheng Fu, Jaroslav Dolezel, Ying Liu, Wenling Zhai, Mahmoud Said, Istvan Molnar, Katerina Holusova, Ruiqi Zhang, Jizhong Wu
Summary: This study identified a new wheat-Dasypyrum villosum translocation line that exhibits stable resistance to wheat sharp eyespot. The introgression of this translocation line into wheat cultivars significantly enhanced their resistance. Through sequencing and marker development, the translocated chromosome can be traced and detected. These findings provide novel germplasm and technological tools for wheat breeding.
Article
Food Science & Technology
Gyongyver Gell, Ildiko Karsai, Zita Berki, Adam Horvath, Christakis George Florides, Zsofia Birinyi, Dalma Nagy-Reder, Balazs Varga, Andras Cseh, Ferenc Bekes, Otto Veisz
Summary: This study aims to investigate the variation of oat protein composition and how the protein compositional parameters change due to irrigation during the grain-filling period, as well as to select safe oat varieties for gluten-free applications.
CURRENT RESEARCH IN FOOD SCIENCE
(2022)