Article
Biochemistry & Molecular Biology
Lin Chen, Feiyan Zhou, Ye Chen, Yongqi Fan, Kangkang Zhang, Qing Liu, Wei Tu, Fujing Jiang, Guangcun Li, Hongbo Zhao, Botao Song
Summary: Freezing severely affects potato production. Understanding the pathways and metabolites that regulate potato's freezing tolerance is beneficial for cultivation and breeding purposes. In this study, it was found that Solanum acaule is more freezing tolerant compared to S. tuberosum. Through RNA-seq and metabolomics analysis, it was discovered that S. acaule activated different genes and enriched certain pathways, such as the hormone signaling pathway involving salicylic acid. Further analysis showed that the content of salicylic acid increased in S. acaule after exposure to cold conditions, leading to improved freezing tolerance. Additionally, the application of salicylic acid to potato enhanced freezing tolerance and increased the expression of HSFC1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Horticulture
Fei Ding, Chuang Wang, Ning Xu, Meiling Wang, Shuoxin Zhang
Summary: The study demonstrates that JA regulates Put biosynthesis by acting on ADC in tomato plants under cold stress, and the process partly relies on MYC2 as a regulator. JA-induced accumulation of Put mitigates cold-induced oxidative stress in plants.
SCIENTIA HORTICULTURAE
(2021)
Article
Horticulture
Xiangli Sun, Zebin Yuan, Bo Wang, Liping Zheng, Jianzhong Tan
Summary: This study demonstrated that exogenous putrescine (Put) supplementation can enhance the synthesis of endogenous arginine (Arg) and polyamines (PAs), while reducing the decomposition rate of PAs in Anthurium andraeanum seedlings under chilling stress. On the other hand, supplementation of D-arginine (D-Arg) and/or difluoromethylornithine (DFMO) inhibited the synthesis of endogenous Arg and its conversion to Put, accelerating the decomposition of PAs. Transcriptional analysis confirmed the differential expression of genes involved in these pathways, supporting the observed physiological effects.
SCIENTIA HORTICULTURAE
(2021)
Article
Plant Sciences
Bailin Liu, Lingshuang Kong, Yu Zhang, Yuncheng Liao
Summary: Potatoes are highly susceptible to elevated temperatures, which can lead to reduced photosynthetic capacity and membrane stability. Short and prolonged heat stress resulted in significant changes in gene expression and metabolite levels related to photosynthesis, heat response, and amino acid biosynthesis, suggesting their potential role in heat tolerance of potatoes. Additionally, heat-induced gene expression in Arabidopsis thaliana and potato leaves overlapped, and certain heat-induced genes in Nicotiana benthamiana increased heat tolerance. This study provides new insights into the molecular mechanisms underlying the potato's response to heat stress.
Article
Horticulture
Jianke Dong, Jingwen Li, Gaofeng Deng, Cheng Chen, Shenglin Jing, Botao Song, Xingkui Cai
Summary: In this study, four frost tolerance loci were identified through field experiments and genetic mapping. Additionally, a genetic linkage map was constructed for chromosome 02, and two loci were found to be associated with frost resistance. These results suggest that the genetic control of frost resistance in potato plants varies under different low temperature conditions.
SCIENTIA HORTICULTURAE
(2023)
Article
Horticulture
Qiulan He, Xi Wu, Ying Liu, Wei Zhang, Jing Liu, Shahzad Munir, Meirong Hai
Summary: In the era of climate change, the rise in temperature can adversely impact the potato growth, yield and even threaten food security. This study provides experimental evidence on the heat tolerance mechanisms in potato plants. The hormone melatonin (MT) has been found to improve potato tolerance to high temperatures, and this study investigates the physiological and molecular mechanisms by introducing exogenous MT to potato seedlings under high-temperature stress. The results show that MT enhances photosynthesis and antioxidant capacity, alleviating the damage caused by high temperature.
SCIENTIA HORTICULTURAE
(2023)
Review
Plant Sciences
Milan Kumar Lal, Rahul Kumar Tiwari, Awadhesh Kumar, Abhijit Dey, Ravinder Kumar, Dharmendra Kumar, Arvind Jaiswal, Sushil Sudhakar Changan, Pinky Raigond, Som Dutt, Satish Kumar Luthra, Sayanti Mandal, Madan Pal Singh, Vijay Paul, Brajesh Singh
Summary: In the face of climate change-induced heat and drought stress, the physiological, biochemical, and molecular responses of potatoes have undergone changes that ultimately affect tuber production and yield. Therefore, it is necessary to comprehensively screen germplasm and apply appropriate methods and strategies to cultivate potato cultivars that can thrive in drought-affected and heat-prone areas.
Article
Biochemistry & Molecular Biology
Alba Alvarez-Morezuelas, Leire Barandalla, Enrique Ritter, Jose Ignacio Ruiz de Galarreta
Summary: In this study, the gene expression differences between control and water-stressed potato plants were analyzed using RNAseq. The variety Agria showed a better stress response compared to Zorba, with more differentially expressed genes (DEGs) detected. The most highly expressed gene under drought conditions was Abscisic acid and environmental stress-inducible protein TAS14-like. Several metabolic pathways and cellular components were found to be affected by water stress, providing valuable information for breeding more tolerant potato varieties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Sophie M. Brouwer, Maja Brus-Szkalej, Ganapathi V. Saripella, Dong Liang, Erland Liljeroth, Laura J. Grenville-Briggs
Summary: Potato early blight, caused by the necrotrophic fungus Alternaria solani, can lead to yield losses of up to 50% if left uncontrolled. Current control strategies using chemical fungicides are not sustainable due to rapid development of fungicide resistance. Lack of understanding of potato defences and resistance mechanisms hinders the development of more sustainable control methods.
Article
Food Science & Technology
Yuhui Liu, Yuanming Li, Zhen Liu, Lei Wang, Zhenzhen Bi, Chao Sun, Panfeng Yao, Junlian Zhang, Jiangping Bai, Yuting Zeng
Summary: Potato is a widely grown crop that can adapt to various geographies and climates. Pigmented potato tubers contain large amounts of flavonoids, which have functional roles and act as antioxidants in the human diet. The effect of altitude on the biosynthesis and accumulation of flavonoids in potato tubers is poorly understood.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Plant Sciences
Jie Song, Hao Wu, Feng He, Jing Qu, Yue Wang, Chunlong Li, Ji-Hong Liu
Summary: This study demonstrates that CBF1 in sweet orange directly regulates the expression of ADC gene and modulates Put synthesis, leading to enhanced cold tolerance. This research sheds light on the transcriptional regulation of Put accumulation and provides insights into the CBF-mediated cold stress response.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Pan Zhao, Lu Liu, Jingjing Cao, Zhiqin Wang, Yonglong Zhao, Naiqin Zhong
Summary: This study revealed that tryptophan can induce resistance against potato common scab by regulating metabolic processes, stimulus response, and biological regulation. The expression patterns of candidate genes involved in salicylic acid and jasmonic acid/ethylene pathways were also analyzed, showing that tryptophan can induce systemic acquired resistance and induced systemic resistance pathways to enhance potato's resistance against common scab.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Tingzhi Huang, Xuesong Zhang, Qianchao Wang, Yirui Guo, Heng Xie, Li Li, Ping Zhang, Junna Liu, Peng Qin
Summary: This study investigates the growth response mechanism of quinoa seedlings 30 days after fertilizer application by extensive metabolome and transcriptome analyses. The results indicate that potassium plays an important role in the growth and development of quinoa, affecting key pathways such as photosynthesis and arginine biosynthesis.
Article
Biotechnology & Applied Microbiology
Shih-Chen Yang, Wan-Wen Ting, I-Son Ng
Summary: The demand for bio-based materials to replace traditional plastics is growing. In this study, a time-efficient method was developed to convert L-arginine into putrescine using whole cell bioconversion. It was found that the key enzymes from different combinations of plasmids can effectively collaborate, achieving a high conversion rate.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Plant Sciences
Farooq Abdul Sattar, Bahget Talat Hamooh, Gordon Wellman, Md Arfan Ali, Saad Hussain Shah, Yasir Anwar, Magdi Ali Ahmed Mousa
Summary: The study screened two potato cultivars, BARI-401 and Spunta, for their tolerance to simulated salinity and drought through in vitro LiCl and mannitol exposure. Results showed that Spunta was able to better maintain shoot length and had higher antioxidant capacity compared to BARI-401 under high LiCl and mannitol concentrations, indicating its greater tolerance to salinity and drought stress. Further field experiments are needed to confirm these findings.
Article
Genetics & Heredity
Kun Li, Ruhao Chen, Zheng Tu, Xianzhou Nie, Botao Song, Changzheng He, Conghua Xie, Bihua Nie
Summary: In this study, a comprehensive investigation on the StDnaJs family was conducted, including their classification, phylogenetic relationships, chromosomal locations, and gene duplication events. The global expression patterns of the members in the 3 HSP families during PVY infection were analyzed. Several genes were found to be significantly up- or downregulated, and further experiments demonstrated their potential roles in the potato-PVY compatible interaction.
Article
Plant Sciences
Ruhao Chen, Zhen Tu, Changzheng He, Xianzhou Nie, Kun Li, Sitian Fei, Botao Song, Bihua Nie, Conghua Xie
Summary: Plant viruses depend on multiple host factors for translation, replication, and movement during infection. The EXA1 gene has been identified as a susceptibility gene for potexvirus, lolavirus, and bacterial and oomycete pathogens. Knockdown of StEXA1 in potato confers resistance to potato virus Y (PVY) in a strain-specific manner. StEXA1 interacts with PVY's HC-Pro protein through a member of eIF4Es and facilitates PVY accumulation through the stress granule-dependent RNA regulatory pathway.
HORTICULTURE RESEARCH
(2022)
Article
Plant Sciences
Shengxuan Liu, Tengfei Liu, Enshuang Wang, Yunxia Cheng, Tiantian Liu, Guogang Chen, Minrui Guo, Botao Song
Summary: In this study, a comprehensive proteome map of potato chloroplast was established using proteomics, revealing key proteins and phosphorylation sites. These results provide a theoretical basis for further research on photosynthesis and starch metabolism.
Article
Biochemistry & Molecular Biology
Enshuang Wang, Tengfei Liu, Xiaomeng Sun, Shenglin Jing, Tingting Zhou, Tiantian Liu, Botao Song
Summary: In this study, the StSP6A protein interaction network in potato was investigated using yeast two-hybrid (Y2H) screening, resulting in the identification of 77 interacting proteins. Three interacting proteins, including Flowering Promoting Factor 1.1 (StFPF1.1), No Flowering in Short Days 1 and 2 (StNFL1 and StNFL2), were further analyzed for expression patterns. The identification of these interacting proteins provides a foundation for future functional studies of StSP6A.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Lin Chen, Hongbo Zhao, Ye Chen, Fujing Jiang, Feiyan Zhou, Qing Liu, Yongqi Fan, Tiantian Liu, Wei Tu, Dirk Walther, Botao Song
Summary: This study identified the key gene SaCBL1-like that confers freezing tolerance in potato and revealed its functional mechanism. This is of great significance for potato breeding in cold climates.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Cell Biology
Gege Li, Jingjing Shao, Yuwen Wang, Tengfei Liu, Yuhao Tong, Shelley Jansky, Conghua Xie, Botao Song, Xingkui Cai
Summary: In this study, the Ry(chc) gene was successfully cloned and demonstrated to confer extreme resistance to Potato virus Y (PVY). The genetic events occurring during the evolution of the Ry(chc) locus were investigated, and a functional marker was developed for marker-assisted selection in breeding programs.
Review
Plant Sciences
Gaurav Moghe, Lars H. Kruse, Maike Petersen, Federico Scossa, Alisdair R. Fernie, Emmanuel Gaquerel, John C. D'Auria
Summary: The ability of plants to chemically modify specialized metabolites is why the plant kingdom has diverse compounds. The addition of an acyl moiety to produce esters and amides is an important chemical modification. Phylogenomics analyses have shown that acyl transfer reactions on small molecules synthesized by plants are performed by enzymes from a few gene families. This review focuses on one of these families, the BAHD acyltransferases, describing their biochemistry, evolutionary origins, and implications in chemical ecology. The BAHD family has critical roles in modifying various specialized metabolites, as confirmed by metabolomic studies and sequencing of plant species. Future research on BAHDs includes their application in synthetic biology and metabolic engineering.
ANNUAL REVIEW OF PLANT BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Lin Chen, Feiyan Zhou, Ye Chen, Yongqi Fan, Kangkang Zhang, Qing Liu, Wei Tu, Fujing Jiang, Guangcun Li, Hongbo Zhao, Botao Song
Summary: Freezing severely affects potato production. Understanding the pathways and metabolites that regulate potato's freezing tolerance is beneficial for cultivation and breeding purposes. In this study, it was found that Solanum acaule is more freezing tolerant compared to S. tuberosum. Through RNA-seq and metabolomics analysis, it was discovered that S. acaule activated different genes and enriched certain pathways, such as the hormone signaling pathway involving salicylic acid. Further analysis showed that the content of salicylic acid increased in S. acaule after exposure to cold conditions, leading to improved freezing tolerance. Additionally, the application of salicylic acid to potato enhanced freezing tolerance and increased the expression of HSFC1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Enshuang Wang, Tingting Zhou, Shenglin Jing, Liepeng Dong, Xiaomeng Sun, Yujie Fan, Yunlong Shen, Tengfei Liu, Botao Song
Summary: Photoperiod plays a crucial role in controlling the formation of sexual or vegetative reproductive organs in potato. StPHYF silenced plants can overcome day-length limitations to tuberize through the activation of florigen StSP3D and other flowering-related genes, leading to an early flowering response. Grafting experiments have revealed the long-distance effect of StPHYF silencing on gene expression in the stolon, including the circadian clock components, flowering-associated MADSs, and tuberization-related regulatory genes. These findings provide a better understanding of the regulatory network of StPHYF in controlling flowering and tuberization during potato reproduction.
Article
Plant Sciences
Tengfei Liu, Md Abu Kawochar, Shengxuan Liu, Yunxia Cheng, Shahnewaz Begum, Enshuang Wang, Tingting Zhou, Tiantian Liu, Xingkui Cai, Botao Song
Summary: Transitory starch and vacuolar sugars are important metabolites in plant leaf cells. The tonoplast sugar transporters (TSTs), such as StTST3.1, play a critical role in sugar uptake and accumulation. In this study, suppressing StTST3.1 resulted in growth retardation, pale green leaves, and impaired photosynthetic performance in potato plants. StTST3.1 was found to be involved in transitory starch turnover and chlorophyll metabolism. These findings highlight the importance of StTST3.1 in normal potato plant growth.
Article
Cell Biology
Wei Tu, Jingcai Li, Jianke Dong, Jianghai Wu, Haibo Wang, Yingtao Zuo, Xingkui Cai, Botao Song
Summary: This study utilized a multi-parental population to perform genetic mapping and molecular marker development for frost tolerance in potatoes. Three candidate regions and six SNP markers associated with frost tolerance were identified. A molecular marker-assisted selection system was established, which provides practical advantages for expanding and improving frost-tolerant germplasm resources in potatoes.
Article
Plant Sciences
Tiantian Liu, Jin Wang, Lin Chen, Shengxuan Liu, Tengfei Liu, Liu Yu, Jingjing Guo, Ye Chen, Yiling Zhang, Botao Song
Summary: Potato production is hindered by cold temperatures, but the plant hormone ABA can improve freezing tolerance. However, the ABA-mediated signaling pathway for freezing tolerance is not well understood. In this study, cold acclimation of potato genotypes was enhanced with increased ABA levels. Exogenous application of ABA or its inhibitor NDGA resulted in enhanced or reduced freezing tolerance, respectively. The transcription factor ScAREB4 was found to be upregulated after cold and ABA treatments, and its overexpression promoted freezing tolerance by increasing trehalose content and antioxidant capacity. The results suggest that increased ABA content is related to freezing tolerance in potato, and ScAREB4 functions as a downstream transcription factor in the ABA signaling pathway.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Plant Sciences
Bingsen Wang, Yuqi Wang, Wenfeng He, Mengshu Huang, Liu Yu, Dong Cheng, Juan Du, Botao Song, Huilan Chen
Summary: Miraculin-like proteins (MLPs), belonging to the Kunitz trypsin inhibitor (KTI) family, have been found to play a role in defending plants against pathogens. In this study, a gene called StMLP1 was identified in potato, which showed increased expression during Ralstonia solanacearum infection. StMLP1 was characterized as an inducible promoter and specifically expressed in vascular bundles. Overexpression of StMLP1 enhanced resistance to R. solanacearum, while inhibiting its expression accelerated the infection. RNA-seq results further confirmed the involvement of StMLP1 in potato immunity.
Article
Plant Sciences
Thomas Naake, Feng Zhu, Saleh Alseekh, Federico Scossa, Leonardo Perez de Souza, Monica Borghi, Yariv Brotman, Tetsuya Mori, Ryo Nakabayashi, Takayuki Tohge, Alisdair R. Fernie
Summary: Plants synthesize specialized metabolites to interact with the environment. This study used genome-wide association studies and quantitative trait locus mapping to identify genetic loci controlling specialized metabolites in Arabidopsis seeds, including glucosinolates, flavonoids, and unknown sulfur-containing compounds.
Article
Plant Sciences
Bingsen Wang, Yuqi Wang, Wenfeng He, Mengshu Huang, Liu Yu, Dong Cheng, Juan Du, Botao Song, Huilan Chen
Summary: This study identified a gene StMLP1 in potato that belongs to the Kunitz trypsin inhibitor (KTI) family and has a role in defending against Ralstonia solanacearum (R. solanacearum) infection. The StMLP1 gene exhibits trypsin inhibitor activity and its overexpression enhances resistance to R. solanacearum, while inhibiting its expression accelerates infection. Furthermore, StMLP1 is involved in potato immunity.
