Application and optimization of CRISPR–Cas9-mediated genome engineering in axolotl (Ambystoma mexicanum)
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Title
Application and optimization of CRISPR–Cas9-mediated genome engineering in axolotl (Ambystoma mexicanum)
Authors
Keywords
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Journal
Nature Protocols
Volume 13, Issue 12, Pages 2908-2943
Publisher
Springer Nature America, Inc
Online
2018-11-13
DOI
10.1038/s41596-018-0071-0
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Note: Only part of the references are listed.- A Survey of Validation Strategies for CRISPR-Cas9 Editing
- (2018) Monica F. Sentmanat et al. Scientific Reports
- The axolotl genome and the evolution of key tissue formation regulators
- (2018) Sergej Nowoshilow et al. NATURE
- Evolved Cas9 variants with broad PAM compatibility and high DNA specificity
- (2018) Johnny H. Hu et al. NATURE
- Enhanced CRISPR/Cas9-mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA
- (2017) Xiquan Liang et al. JOURNAL OF BIOTECHNOLOGY
- Genome editing using CRISPR/Cas9-based knock-in approaches in zebrafish
- (2017) Shahad Albadri et al. METHODS
- Efficient gene knockin in axolotl and its use to test the role of satellite cells in limb regeneration
- (2017) Ji-Feng Fei et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Reading and editing the Pleurodeles waltl genome reveals novel features of tetrapod regeneration
- (2017) Ahmed Elewa et al. Nature Communications
- A Tissue-Mapped Axolotl De Novo Transcriptome Enables Identification of Limb Regeneration Factors
- (2017) Donald M. Bryant et al. Cell Reports
- Efficient identification of CRISPR/Cas9-induced insertions/deletions by direct germline screening in zebrafish
- (2016) Isabel Brocal et al. BMC GENOMICS
- A simplified method for identifying early CRISPR-induced indels in zebrafish embryos using High Resolution Melting analysis
- (2016) Éric Samarut et al. BMC GENOMICS
- Maximizing mutagenesis with solubilized CRISPR-Cas9 ribonucleoprotein complexes
- (2016) Alexa Burger et al. DEVELOPMENT
- Rapid and efficient analysis of gene function using CRISPR-Cas9 in Xenopus tropicalis founders
- (2016) Mitsuki Shigeta et al. GENES TO CELLS
- Delivery of Cas9 Protein into Mouse Zygotes through a Series of Electroporation Dramatically Increases the Efficiency of Model Creation
- (2016) Wenbo Wang et al. Journal of Genetics and Genomics
- The Neisseria meningitidis CRISPR-Cas9 System Enables Specific Genome Editing in Mammalian Cells
- (2016) Ciaran M Lee et al. MOLECULAR THERAPY
- Streptococcus thermophilus CRISPR-Cas9 Systems Enable Specific Editing of the Human Genome
- (2016) Maximilian Müller et al. MOLECULAR THERAPY
- Analyzing CRISPR genome-editing experiments with CRISPResso
- (2016) Luca Pinello et al. NATURE BIOTECHNOLOGY
- Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair
- (2016) Xiangjun He et al. NUCLEIC ACIDS RESEARCH
- Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes
- (2016) Sergei Svitashev et al. Nature Communications
- Homology Directed Knockin of Point Mutations in the Zebrafish tardbp and fus Genes in ALS Using the CRISPR/Cas9 System
- (2016) Gary Alan Barclay Armstrong et al. PLoS One
- Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
- (2015) Bernd Zetsche et al. CELL
- Intron targeting-mediated and endogenous gene integrity-maintaining knockin in zebrafish using the CRISPR/Cas9 system
- (2015) Jia Li et al. CELL RESEARCH
- High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes
- (2015) A. Paix et al. GENETICS
- Engineered CRISPR-Cas9 nucleases with altered PAM specificities
- (2015) Benjamin P. Kleinstiver et al. NATURE
- In vivo genome editing using Staphylococcus aureus Cas9
- (2015) F. Ann Ran et al. NATURE
- Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition
- (2015) Benjamin P Kleinstiver et al. NATURE BIOTECHNOLOGY
- Comparison of T7E1 and Surveyor Mismatch Cleavage Assays to Detect Mutations Triggered by Engineered Nucleases
- (2015) Léna Vouillot et al. G3-Genes Genomes Genetics
- Efficient Multiple Genome Modifications Induced by the crRNAs, tracrRNA and Cas9 Protein Complex in Zebrafish
- (2015) Hirohito Kotani et al. PLoS One
- Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish
- (2015) Yu Hisano et al. Scientific Reports
- Development and Applications of CRISPR-Cas9 for Genome Engineering
- (2014) Patrick D. Hsu et al. CELL
- Highly efficient targeted mutagenesis in axolotl using Cas9 RNA-guided nuclease
- (2014) G. P. Flowers et al. DEVELOPMENT
- Precise and efficient genome editing in zebrafish using the CRISPR/Cas9 system
- (2014) U. Irion et al. DEVELOPMENT
- A CRISPR view of development
- (2014) Melissa M. Harrison et al. GENES & DEVELOPMENT
- Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins
- (2014) S. Kim et al. GENOME RESEARCH
- Improving CRISPR-Cas nuclease specificity using truncated guide RNAs
- (2014) Yanfang Fu et al. NATURE BIOTECHNOLOGY
- CRISPR-Cas systems for editing, regulating and targeting genomes
- (2014) Jeffry D Sander et al. NATURE BIOTECHNOLOGY
- Optimized axolotl (Ambystoma mexicanum) husbandry, breeding, metamorphosis, transgenesis and tamoxifen-mediated recombination
- (2014) Shahryar Khattak et al. Nature Protocols
- Efficient Mutagenesis by Cas9 Protein-Mediated Oligonucleotide Insertion and Large-Scale Assessment of Single-Guide RNAs
- (2014) James A. Gagnon et al. PLoS One
- The new frontier of genome engineering with CRISPR-Cas9
- (2014) J. A. Doudna et al. SCIENCE
- RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein
- (2014) Jeong-Soo Lee et al. G3-Genes Genomes Genetics
- CRISPR-Mediated Genomic Deletion of Sox2 in the Axolotl Shows a Requirement in Spinal Cord Neural Stem Cell Amplification during Tail Regeneration
- (2014) Ji-Feng Fei et al. Stem Cell Reports
- One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering
- (2013) Haoyi Wang et al. CELL
- Fundamental Differences in Dedifferentiation and Stem Cell Recruitment during Skeletal Muscle Regeneration in Two Salamander Species
- (2013) Tatiana Sandoval-Guzmán et al. Cell Stem Cell
- Efficient identification of TALEN-mediated genome modifications using heteroduplex mobility assays
- (2013) Satoshi Ota et al. GENES TO CELLS
- Simple and efficient CRISPR/Cas9-mediated targeted mutagenesis inXenopus tropicalis
- (2013) Takuya Nakayama et al. GENESIS
- Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9–sgRNA Ribonucleoproteins
- (2013) Seung Woo Cho et al. GENETICS
- Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases
- (2013) Y. H. Sung et al. GENOME RESEARCH
- Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair
- (2013) T. O. Auer et al. GENOME RESEARCH
- High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
- (2013) Yanfang Fu et al. NATURE BIOTECHNOLOGY
- Cas9 as a versatile tool for engineering biology
- (2013) Prashant Mali et al. NATURE METHODS
- Single-stranded DNA library preparation for the sequencing of ancient or damaged DNA
- (2013) Marie-Theres Gansauge et al. Nature Protocols
- RNA-Guided Human Genome Engineering via Cas9
- (2013) P. Mali et al. SCIENCE
- Multiplex Genome Engineering Using CRISPR/Cas Systems
- (2013) L. Cong et al. SCIENCE
- ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
- (2013) Thomas Gaj et al. TRENDS IN BIOTECHNOLOGY
- Comparative RNA-seq Analysis in the Unsequenced Axolotl: The Oncogene Burst Highlights Early Gene Expression in the Blastema
- (2013) Ron Stewart et al. PLoS Computational Biology
- Germline Transgenic Methods for Tracking Cells and Testing Gene Function during Regeneration in the Axolotl
- (2013) Shahryar Khattak et al. Stem Cell Reports
- TALENs: a widely applicable technology for targeted genome editing
- (2012) J. Keith Joung et al. NATURE REVIEWS MOLECULAR CELL BIOLOGY
- Inducible genetic system for the axolotl
- (2012) J. L. Whited et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity
- (2012) M. Jinek et al. SCIENCE
- Simple Methods for Generating and Detecting Locus-Specific Mutations Induced with TALENs in the Zebrafish Genome
- (2012) Timothy J. Dahlem et al. PLoS Genetics
- Gene expression profile of the regeneration epithelium during axolotl limb regeneration
- (2011) Leah J. Campbell et al. DEVELOPMENTAL DYNAMICS
- Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega
- (2011) F. Sievers et al. Molecular Systems Biology
- Double indexing overcomes inaccuracies in multiplex sequencing on the Illumina platform
- (2011) Martin Kircher et al. NUCLEIC ACIDS RESEARCH
- High Cleavage Efficiency of a 2A Peptide Derived from Porcine Teschovirus-1 in Human Cell Lines, Zebrafish and Mice
- (2011) Jin Hee Kim et al. PLoS One
- Regulation of Sox2 in the pre-placodal cephalic ectoderm and central nervous system by enhancer N-4
- (2010) Yuka Saigou et al. DEVELOPMENT GROWTH & DIFFERENTIATION
- Improving Bioscience Research Reporting: The ARRIVE Guidelines for Reporting Animal Research
- (2010) Carol Kilkenny et al. PLOS BIOLOGY
- Enzymatic assembly of DNA molecules up to several hundred kilobases
- (2009) Daniel G Gibson et al. NATURE METHODS
- Alternative translation start sites and hidden coding potential of eukaryotic mRNAs
- (2008) Alex V. Kochetov BIOESSAYS
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