Gold-coated nanoporous polycarbonate track-etched solid platform for the rapid detection of mesothelin

Gold nanolayercoated polycarbonate track-etched membrane (PCTE)-based microfluidic electrochemical biosensor grown on a highly interactive sandwich-like nanolayer has been designed. The gold nanolayer behaves like a sandwich further underwent thiol modification, EDC/NHS chemistry endowed excellent interaction with the immobilized biomolecule. The gold-coated surface of the biosensor has been functionalized with the anti-mesothelin antibody for the selective detection of the corresponding antigen. Specific antigen-antibody interaction has been recorded via ionic current vs. applied voltage (keeping the value in the range of 0.1-1.5V). The biosensor exhibit adequate changes in ionic current upon the application of different concentrations (100ngmL(-1) to 10agmL(-1)) of mesothelin antigen and present excellent sensitivity, such as 0.011agmL(-1)cm(-1) (for 50-nm pore), 0.027agmL(-1)cm(-1) (for 80-nm pore), and 0.017agmL(-1)cm(-1) (for 100-nm pore). The antigen concentration was kept variable (six concentrations) against fixed antibody concentration.