Article
Biochemistry & Molecular Biology
Miriama Simunkova, Zuzana Barbierikova, Klaudia Jomova, Lenka Hudecova, Peter Lauro, Saleh H. Alwasel, Ibrahim Alhazza, Christopher J. Rhodes, Marian Valko
Summary: Kaempferol, a flavonoid found in tea, vegetables, and fruits, has demonstrated efficacy in treating various cancers and leukemia. Studies have focused on its prooxidant properties and activation of pro-apoptotic pathways. Research investigated the antioxidant vs. prooxidant properties of flavonoids in the presence of redox-active copper (II) ions during the Fenton reaction. The results suggest that Cu-kaempferol complexes may provide anticancer activity and suggest potential therapeutic use in oxidative stress-related medical conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Jianbo Fu, Qingxia Yang, Yongchao Luo, Song Zhang, Jing Tang, Ying Zhang, Hongning Zhang, Hanxiang Xu, Feng Zhu
Summary: Label-free quantification (LFQ) is an important technique in proteomics due to its broad proteome coverage and enhanced reproducibility. In this study, an R package EVALFQ was developed to evaluate and discover well-performing LFQ chains from over 3000 options.
BRIEFINGS IN BIOINFORMATICS
(2022)
Article
Biochemical Research Methods
Jianbo Fu, Qingxia Yang, Yongchao Luo, Song Zhang, Jing Tang, Ying Zhang, Hongning Zhang, Hanxiang Xu, Feng Zhu
Summary: The label-free quantification (LFQ) is a powerful technique in proteomics due to its broad proteome coverage, great dynamic ranges, and enhanced analytical reproducibility. However, determining the well-performing LFQ chain is challenging due to the dependence on data and the diverse possibilities. In this study, a unique R package EVALFQ was developed for performance evaluation of >3000 LFQ chains, utilizing multiple criteria, spiking proteins, and comprehensive assessment. This package is expected to attract broad interests in the field of proteomic quantification.
BRIEFINGS IN BIOINFORMATICS
(2023)
Article
Multidisciplinary Sciences
Julia P. Schessner, Vincent Albrecht, Alexandra K. Davies, Pavel Sinitcyn, Georg H. H. Borner
Summary: The Dynamic Organellar Maps (DOMs) approach combines cell fractionation and shotgun-proteomics to analyze protein subcellular localization. By using data-independent acquisition (DIA) mass spectrometry, DIA-DOMs achieve deeper coverage and improve precision and reproducibility compared to previous methods. A software tool called DOM-ABC is introduced for analyzing profiling data. DIA-DOMs are applied to identify subcellular localization changes in HeLa cells, revealing cycles of Golgi proteins through endosomes. This workflow offers a superior label-free spatial proteomics approach for systematic phenotype discovery.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Shuang Zhang, Behafarid Ghalandari, Aiting Wang, Sijie Li, Youming Chen, Qingwen Wang, Lai Jiang, Xianting Ding
Summary: Mass spectrometry has become a mainstream technique for label-free proteomics, but the proteomic coverage for trace samples is limited by adsorption loss during repeated elution. In this study, superparamagnetic composite nanoparticles functionalized with molecular glues were used to enrich proteins in trace human biofluid, and a streamlined workflow method was proposed to achieve unbiased protein capture, digestion, and elution. This method greatly simplified sample preparation steps, minimized adsorption loss, and improved protein coverage for label-free proteomics with trace samples.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Mahci Al Bashera, Ashik Mosaddik, Gaber El-Saber Batiha, Mohammed Alqarni, Md. Ashraful Islam, George D. Zouganelis, Athanasios Alexiou, Ronok Zahan
Summary: The study evaluated the anti-inflammatory and antioxidant effects of extracts from Oldenlandia corymbosa L., with CDCME showing the most prominent effects in preventing inflammation. This validates the traditional use of the plant for pain relief.
APPLIED SCIENCES-BASEL
(2021)
Article
Agriculture, Multidisciplinary
Soniya Amini, Abbas Hassani, Abolfazl Alirezalu, Ramin Maleki
Summary: The study revealed significant variations in TFC, antioxidant activity, TCC, and beta-carotene content among different mullein species. Verbascum saccatum and V. songaricum were found to be particularly rich in total flavonoids and exhibited the highest antioxidant capacity. The results provide valuable information for breeding strategies and selecting the best species with high phenolic compound content for producing natural antioxidants for medical and pharmaceutical use.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Chemistry, Analytical
Jake T. T. Kline, Michael W. W. Belford, Jingjing J. Huang, Joseph B. B. Greer, David Bergen, Ryan T. T. Fellers, Sylvester M. M. Greer, David M. M. Horn, Vlad Zabrouskov, Romain Huguet, Cornelia L. L. Boeser, Kenneth R. R. Durbin, Luca Fornelli
Summary: Label-free quantification of intact proteoforms in the 0-30kDa mass range is typically limited by sample complexity. In this study, gas-phase fractionation (GPF) via field asymmetric ion mobility spectrometry (FAIMS) was applied to increase the number of identified and quantified proteoforms in Escherichia coli. The use of multiple FAIMS compensation voltages in the same experiment improved speed without sacrificing quantification accuracy, resulting in enhanced label-free quantification.
ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Ri-Si Wang, Pan-Hao Dong, Xi-Xiang Shuai, Ming-Shun Chen
Summary: This study evaluated the nutritional composition and antioxidant activity of different cultivars of black mulberry fruits, and found that Qiong46 (M-2), Yuebanguo (M-4), and Heizhenzhu (M-10) were recommended for commercial cultivation.
Review
Chemistry, Applied
Nan Shen, Tongfei Wang, Quan Gan, Sian Liu, Li Wang, Biao Jin
Summary: Flavonoids are natural polyphenol substances abundant in various plant-based foods. They have significant biological activities in both plants and humans, such as antioxidant, antimicrobial, and anti-inflammatory effects. However, research on their distribution, biosynthesis, and health benefits remains limited. Future opportunities in flavonoid research include biotechnology, therapeutic phytoproducts, and exploring dietary flavonoid bioavailability.
Article
Biochemistry & Molecular Biology
Lu Yang, Jiuyang Zhao, Shaoli Fan, Jinfa Liao, Yicun Chen, Yangdong Wang
Summary: Mulberry leaves collected after frost have superior medicinal properties. This study conducted metabolic profiling analyses on two types of mulberry leaves harvested at different times and identified over 100 compounds. The results showed that frost had different effects on the accumulation of metabolites in Morus nigra L. and Morus alba L., and picking time also influenced the levels of DNJ alkaloids and flavonoids in mulberry leaves.
Article
Plant Sciences
Milena Polumackanycz, Marek Wesolowski, Agnieszka Viapiana
Summary: By comparing the leaves of white and black mulberry, it was found that black mulberry leaves have higher levels of flavonoids and phenolic acids, while white mulberry leaves contain higher levels of various phenolic compounds in water extracts, with gallic acid being the highest. There is a strong relationship between antioxidant activity and total phenolics and flavonoids, indicating the crucial role of phenolic compounds as antioxidant agents in white and black mulberry leaves.
PLANT FOODS FOR HUMAN NUTRITION
(2021)
Review
Biochemistry & Molecular Biology
Umme Tamanna Ferdous, Zetty Norhana Balia Yusof
Summary: Flavonoids, important plant chemicals, are found not only in terrestrial plants but also in microalgae and seaweeds, offering potential for novel anticancer compounds with unique chemical structures and bioactivities. Flavonoids act as antioxidants and have the ability to induce apoptosis and autophagy in cancer cells. However, research on the anticancer properties of flavonoids is mainly focused on terrestrial plants.
Article
Biochemistry & Molecular Biology
Neha Kukreti, Havagiray R. Chitme, Vinay K. Varshney, Basel A. Abdel-Wahab, Masood Medleri Khateeb, Mohammed Shafiuddin Habeeb
Summary: This study examined the antioxidant activities of Skimmia anquetilia using in vitro and in vivo models. The plant was found to contain multiple important active constituents with strong antioxidant, hepatoprotective, and nephroprotective activities. Further research on specific active constituents is recommended.
Article
Chemistry, Multidisciplinary
Thy Truong, Kei G. I. Webber, S. Madisyn Johnston, Hannah Boekweg, Caleb M. Lindgren, Yiran Liang, Alissia Nydegger, Xiaofeng Xie, Tsz-Ming Tsang, D. A. Dasun N. Jayatunge, Joshua L. Andersen, Samuel H. Payne, Ryan T. Kelly
Summary: We developed a new method called wide window acquisition (WWA) that combines efficient sample preparation and ultra-low-flow liquid chromatography to quantify >3,000 proteins from single cells in rapid label-free analyses. WWA increased the number of identified proteins by about 40% compared to standard data-dependent acquisition. Utilizing this platform, we compared protein expression in HeLa cells with and without the essential autophagy gene atg9a, and found significant changes in 268 proteins related to innate immunity, vesicle trafficking, and protein degradation.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)