Article
Plant Sciences
Yuqin Zhang, Yuanyuan Hu, Zhiqin Wang, Xiaodi Lin, Zihui Li, Yafang Ren, Jie Zhao
Summary: This study investigates the biological functions of AtTIM22-2 in Arabidopsis. Loss of AtTIM22-2 function leads to defects in embryo and endosperm development, resulting in seed abortion. AtTIM22-2 interacts with AtTIM23-2, AtB14.7, and AT5G27395, participating in mitochondrial biogenesis and maintaining mitochondrial functions and ROS homeostasis.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Multidisciplinary Sciences
Uwe Schulte, Fabian den Brave, Alexander Haupt, Arushi Gupta, Jiyao Song, Catrin S. S. Mueller, Jeannine Engelke, Swadha Mishra, Christoph Martensson, Lars Ellenrieder, Chantal Priesnitz, Sebastian P. P. Straub, Kim Nguyen Doan, Bogusz Kulawiak, Wolfgang Bildl, Heike Rampelt, Nils Wiedemann, Nikolaus Pfanner, Bernd Fakler, Thomas Becker
Summary: This study presents a quantitative mapping of mitochondrial protein assemblies using high-resolution complexome profiling. The results reveal the complex organization of mitochondrial protein assemblies involved in various cellular processes. It also identifies quality-control factors and elucidates a constitutive pathway for preprotein removal.
Article
Biochemistry & Molecular Biology
Yunxiao Dou, Yan Tan
Summary: Amyloid-beta (A beta) peptide is accumulated in mitochondria and plays a central role in Alzheimer's disease (AD) development. Exposure to aggregated A beta can damage mitochondria and dysregulate mitophagy. This study directly changed the A beta content in mitochondria and found that increased mitochondrial A beta enhances mitophagy levels. Overexpression of PreP can reverse mitochondrial A beta-induced mitophagy by regulating reactive oxygen species (ROS) and the mitochondrial membrane potential. These findings provide novel insight into the role of mitochondria-specific A beta in AD pathophysiology.
Article
Biochemistry & Molecular Biology
Mengjiao Zhong, Chen Sun, Bing Zhou
Summary: Artemisinin, derived from the medicinal plant Artemisia annua L., is used as an antimalarial drug. It has been found to inhibit insect feeding behavior and growth, but it is unclear if these effects are independent. This study demonstrated that artemisinin repels larval feeding in fruit flies, but this feeding inhibition does not fully explain its toxicity on larval growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Hua Gong, Qiangzhe Zhang, Anvita Komarla, Shuyan Wang, Yaou Duan, Zhidong Zhou, Fang Chen, Ronnie H. Fang, Sheng Xu, Weiwei Gao, Liangfang Zhang
Summary: Natural cell membranes from various sources have been used to coat nanomaterials for functionalization, but intracellular membranes from eukaryotic organelles remain unexplored. This study successfully coats the outer mitochondrial membrane onto nanoparticles and FETs, showing potential for protecting cells from cell death and detecting specific molecules. The results suggest that intracellular membranes can be used as coating materials for novel biointerfacing applications.
Article
Medicine, Research & Experimental
Xiaomin Liao, Xianxian Ruan, Xianbin Wu, Zhejun Deng, Shanyu Qin, Haixing Jiang
Summary: This study aimed to explore the association between Timm13 and liver fibrosis. Gene expression profiling and network analysis revealed that Timm13 is a hub gene in the protein-protein interaction network. The mRNA levels of Timm13 were found to decrease in fibrotic liver tissue. Silencing Timm13 significantly reduced the expression of fibrotic and apoptosis-related genes.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Ulfat Mohd Hanif Sayyed, Radhakrishnan Mahalakshmi
Summary: The article discusses the unique properties of the human mitochondrial outer membrane and the importance of the TOM complex. It highlights the recent research progress and the potential impact on alleviating human diseases.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Victor V. Lemeshko
Summary: Recently reported kinase-linked mild depolarization of mitochondria may be a crucial component of the mitochondrial anti-aging program. However, the current measurement methods for mitochondrial inner membrane potential ignore the possibility of outer membrane potential generation. The experimental data reported in this study may reflect changes in the positive outer membrane potential generated by VDAC-kinase complexes.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2022)
Review
Biochemistry & Molecular Biology
Jakob D. Busch, Laura F. Fielden, Nikolaus Pfanner, Nils Wiedemann
Summary: Biogenesis of mitochondria involves the import of numerous precursor proteins across the mitochondrial membranes. Different import pathways exist, ranging from presequence-directed pathway to pathways using internal or carboxy-terminal targeting signals. Recent studies have revealed the structural organization of membrane-embedded preprotein translocases and their dynamic interactions with other cellular machineries. These insights provide valuable knowledge about the mechanisms of mitochondrial protein import.
Article
Biology
Saskia Roedl, Fabian den Brave, Markus Raeschle, Buesra Kizmaz, Svenja Lenhard, Carina Groh, Hanna Becker, Jannik Zimmermann, Bruce Morgan, Elke Richling, Thomas Becker, Johannes M. Herrmann
Summary: Mitochondria undergo proteomic changes during transitions between different metabolic states, with cytosolic ubiquitin conjugase Ubc8 playing a crucial role in the remodeling process when cells transition from respiratory to fermentative conditions. Ubc8 promotes the assembly of the outer membrane translocase of mitochondria (TOM) and increases the levels of its cytosol-exposed receptor subunit Tom22. Ubc8 deficiency results in compromised protein import into mitochondria and reduced steady-state levels of mitochondrial proteins.
LIFE SCIENCE ALLIANCE
(2023)
Article
Biology
Hong Qi, Yu-Song Yin, Zhi-Yong Yin, Xiang Li, Jian-Wei Shuai
Summary: Recent studies have found a close relationship between apoptosis and inflammation, but the mechanism linking them through mitochondrial membrane permeabilization is still unclear. In this study, a mathematical model consisting of four functional modules was constructed. Bifurcation analysis revealed bistability caused by Bcl-2 family member interaction, and time series showed a 30-minute time difference between Cyt c and mtDNA release, consistent with previous research. The model predicted that Bax aggregation kinetics determines whether cells undergo apoptosis or inflammation, and altering the inhibitory effect of caspase 3 on IFN-β production allows both apoptosis and inflammation to occur concurrently. This work provides a theoretical framework for exploring the mechanism of mitochondrial membrane permeabilization in controlling cell fate.
JOURNAL OF THEORETICAL BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Johannes M. Herrmann, Yury S. Bykov
Summary: Mitochondrial proteins are synthesized in the cytosol and targeted to different compartments within the mitochondria through specific targeting signals in their sequence. The mitochondrial import system is highly similar in yeast, mammalian cells, and even less closely related eukaryotes such as plants and trypanosomes.
Retraction
Oncology
Shivani Ponnala, Chandramu Chetty, Krishna Kumar Veeravalli, Dzung H. Dinh, Jeffrey D. Klopfenstein, Jasti S. Rao
Summary: The study demonstrates that silencing MMP-9 in combination with uPAR/cathepsin B can switch the metabolic pathway of glioma cells from glycolysis to oxidative phosphorylation, leading to mitochondrial outer membrane permeabilization and potential therapeutic benefits.
INTERNATIONAL JOURNAL OF ONCOLOGY
(2021)
Article
Biology
Layla Drwesh, Benjamin Heim, Max Graf, Linda Kehr, Lea Hansen-Palmus, Mirita Franz-Wachtel, Boris Macek, Hubert Kalbacher, Johannes Buchner, Doron Rapaport
Summary: This study reconstituted the early cytosolic steps of signal-anchored (SA) protein biogenesis and identified molecular (co)chaperones, including Hsp70, Hsp90, and Hsp40 family co-chaperones, that interact with newly synthesized SA proteins. These interactions are mediated by the hydrophobic transmembrane segments of the SA proteins. The study also demonstrated the inhibitory effect of interfering with these interactions on SA protein biogenesis and successfully reconstituted the transfer of peptides from Hsp70 chaperone to the mitochondrial Tom70 receptor in vitro.
Article
Multidisciplinary Sciences
Jordan M. Johnson, Alek D. Peterlin, Enrique Balderas, Elahu G. Sustarsic, J. Alan Maschek, Marisa J. Lang, Alejandro Jara-Ramos, Vanja Panic, Jeffrey T. Morgan, Claudio J. Villanueva, Alejandro Sanchez, Jared Rutter, Irfan J. Lodhi, James E. Cox, Kelsey H. Fisher-Wellman, Dipayan Chaudhuri, Zachary Gerhart-Hines, Katsuhiko Funai
Summary: Phosphatidylethanolamine (PE) modulates the proton conductance of UCP1, regulating thermogenesis in brown adipose tissue (BAT).