Article
Chemistry, Analytical
Zachary Klamer, Brian Haab
Summary: MotifFinder is a novel software algorithm for glycan-array analysis that accurately interprets data from diverse datasets, enabling the identification of binding constants for distinct motifs and providing broader views of lectin specificity. This advancement promises to enhance studies on protein-glycan interactions.
ANALYTICAL CHEMISTRY
(2021)
Review
Cell Biology
Franziska Krautter, Asif J. Iqbal
Summary: The recruitment of leukocytes is a complex process involving interactions between proteins expressed by the endothelium and circulating leukocytes. Recent research has expanded our understanding of these interactions, identifying novel lectins and regulatory pathways. Targeting glycans and glycan-binding proteins could offer new therapeutic strategies for modulating leukocyte recruitment and transmigration during inflammation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Qingyang Shi, Jiahuan Chen, Xiaodong Zou, Xiaochun Tang
Summary: Cholesterol homeostasis is important in understanding various diseases, and this review focuses on cholesterol synthesis, regulation, trafficking, and the role of lipid transfer proteins at organelle contact sites.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Asif J. Iqbal, Franziska Krautter, Isobel A. Blacksell, Rachael D. Wright, Shani N. Austin-Williams, Mathieu-Benoit Voisin, Mohammed T. Hussain, Hannah L. Law, Toshiro Niki, Mitsuomi Hirashima, Michele Bombardieri, Costantino Pitzalis, Alok Tiwari, Gerard B. Nash, Lucy Norling, Dianne Cooper
Summary: This study investigates the role of immunomodulatory lectin Galectin-9 (Gal-9) in neutrophil recruitment. The results show that Gal-9 is upregulated in inflamed blood vessels and released by activated endothelial cells. Gal-9 binding induces neutrophil activation and can enhance their interaction with endothelial cells. Both soluble and immobilized Gal-9 function as adhesion molecules and can capture neutrophils. These findings provide insights into the importance of Gal-9 in leukocyte recruitment during inflammation.
Article
Chemistry, Multidisciplinary
Luminita Claudia Miclea, Mona Mihailescu, Nicolae Tarba, Ana-Maria Brezoiu, Ana Maria Sandu, Raul-Augustin Mitran, Daniela Berger, Cristian Matei, Mihaela Georgeta Moisescu, Tudor Savopol
Summary: This study investigates the intracellular distribution of folate-conjugated mesoporous silica nanoparticles and demonstrates that the conjugation of folate increases the efficiency of nanoparticle uptake and promotes their preferential localization near the nucleus.
Article
Chemistry, Multidisciplinary
Xiaocui Zheng, Yiran Li, Tongxiao Cui, Jing Yang, Xiangfeng Meng, Haiqi Wang, Liusheng Chen, Jian He, Nan Chen, Liying Meng, Lin Ding, Ran Xie
Summary: Executing precise glycan editing is crucial for understanding glycan-related biological processes and improving disease therapy. This study introduces a versatile and traceless glycan modification method that allows customization of targeted protein glycoforms through chemical and photoregulation. The method minimizes interference and enables precise temporal control of editing events.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Letter
Medicine, General & Internal
Theoharis C. Theoharides, Irene Tsilioni
Summary: The article points out that mast cells, as a unique tissue immune cell, can orchestrate the immune response by secreting proinflammatory molecules. It highlights the degranulation of mast cells in the lungs of deceased patients with severe Covid-19, as well as the elevated levels of mast cell-derived proteolytic enzymes in patients' serum.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Microbiology
Huntly M. Morrison, Julia Craft, Rafael Rivera-Lugo, Jeffery R. Johnson, Guillaume R. Golovkine, Samantha L. Bell, Claire E. Dodd, Erik Van Dis, Wandy L. Beatty, Shally R. Margolis, Teresa Repasy, Isaac Shaker, Angus Y. Lee, Russell E. Vance, Sarah A. Stanley, Robert O. Watson, Nevan J. Krogan, Daniel A. Portnoy, Bennett H. Penn, Jeffery S. Cox
Summary: Intracellular bacterial pathogens rely on damaging host cell membranes for nutrient access, but this also triggers immune responses. Galectin-3, -8, and -9 recognize membrane damage and activate autophagy. However, their role in vivo during bacterial infection remains unclear. In this study, mice lacking Gal-3, -8, and -9 showed no defects in acute infection resistance but had impaired autophagy and modest susceptibility to chronic infection.
Review
Biochemistry & Molecular Biology
Abigail E. Reeves, Mia L. Huang
Summary: Glycosylation is a common modification that plays important roles in protein function, but studying the interactions and consequences of glycan-binding protein (GBP) recognition can be challenging. Proximity labeling, a recent experimental technique, has emerged as a powerful strategy to overcome these challenges by tagging interacting molecules in cells for subsequent identification. In this article, we discuss the applications of proximity labeling in the study of different GBP classes over the past five years.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2023)
Article
Immunology
Sofia Gargani, Niki Lourou, Christina Arapatzi, Dimitris Tzanos, Marania Saridaki, Esmeralda Dushku, Margarita Chatzimike, Nikolaos D. Sidiropoulos, Margarita Andreadou, Vasileios Ntafis, Pantelis Hatzis, Vassiliki Kostourou, Dimitris L. Kontoyiannis
Summary: The RNA-binding protein hnRNPD/AUF1 does not act as a general anti-inflammatory factor in innate immune cells, but rather plays more specialized roles in regulating specific innate immune cell transitions to support tissue infiltration and remodeling processes.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biology
Sumnima Singh, Patricia Bastos-Amador, Jessica Ann Thompson, Mauro Truglio, Bahtiyar Yilmaz, Silvia Cardoso, Daniel Sobral, Miguel P. Soares
Summary: This study shows that deletion of the Ggta1 gene can shape the composition of gut microbiota via an Ig-dependent mechanism, where antibodies play a role in reducing microbiota pathogenicity. This suggests that loss of GGTA1 function in ancestral primates might have been influenced by microbiota interactions.
Article
Biochemistry & Molecular Biology
Ji Young Hyun, Sanggil Kim, Chang-Hee Lee, Hyun Soo Lee, Injae Shin
Summary: This study presents a general method for efficient preparation of homogeneous glycoproteins using genetic code expansion and chemoselective ligation techniques. The glycoproteins produced through this method can recognize mammalian cell-surface lectins and enter cells, showing potential for biomedical applications.
ACS CHEMICAL BIOLOGY
(2021)
Article
Microbiology
Marisa Dilucca, Saray Ramos, Kateryna Shkarina, Jose Carlos Santos, Petr Broz
Summary: Inflammasomes are cytosolic signaling complexes activated by pathogen-derived ligands or endogenous danger signals, leading to cell death and cytokine release. Interferons play a regulatory role in inflammasome activation through GBPs and can confer protection against pathogens by inducing pyroptosis via caspase-4.
Article
Microbiology
Antonia R. Bass, Marisa S. Egan, Jasmine Alexander-Floyd, Natasha Lopes Fischer, Jessica Doerner, Sunny Shin
Summary: The inflammasome is critical for the host defense against intracellular bacterial pathogens. IFN and GBP1 play important roles in the inflammasome response to Legionella pneumophila infection in human macrophages, enhancing cytokine release and facilitating bacterial access to the host cell cytosol.
Article
Multidisciplinary Sciences
Wen Cui, Elisabeth Braun, Wei Wang, Jinhong Tang, Yanyan Zheng, Benjamin Slater, Na Li, Cheng Chen, Qingxiang Liu, Bin Wang, Xiu Li, Yinkai Duan, Yunjie Xiao, Ruijiao Ti, Dominik Hotter, Xiaoyun Ji, Lei Zhang, Jun Cui, Yong Xiong, Daniel Sauter, Zefang Wang, Frank Kirchhoff, Haitao Yang
Summary: This study presents crystal structures of Guanylate-binding proteins and reveals a GTP-induced dimerization mode that is likely conserved among all GBPs, providing insights into the molecular determinants of their antiviral function.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Kurt Drickamer, Maureen E. Taylor
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2015)
Article
Biochemistry & Molecular Biology
Sabine A. F. Jegouzo, Hadar Feinberg, Tabassum Dungarwalla, Kurt Drickamer, William I. Weis, Maureen E. Taylor
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Article
Biochemistry & Molecular Biology
Neela D. S. Rambaruth, Sabine A. F. Jegouzo, Hayley Marlor, Maureen E. Taylor, Kurt Drickamer
Article
Biochemistry & Molecular Biology
Hadar Feinberg, Neela D. S. Rambaruth, Sabine A. F. Jegouzo, Kristian M. Jacobsen, Rasmus Djurhuus, Thomas B. Poulsen, William I. Weis, Maureen E. Taylor, Kurt Drickamer
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Biochemistry & Molecular Biology
Alia dos Santos, Andreas Hadjivasiliou, Felipe Ossa, Novandy K. Lim, Aylin Turgut, Maureen E. Taylor, Kurt Drickamer
Article
Biochemistry & Molecular Biology
Hadar Feinberg, Sabine A. F. Jegouzo, Maximus J. Rex, Kurt Drickamer, William I. Weis, Maureen E. Taylor
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Maureen E. Taylor, Kurt Drickamer
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Sabine A. F. Jegouzo, Adrian Quintero-Martinez, Xiangyu Ouyang, Alia dos Santos, Maureen E. Taylor, Kurt Drickamer
Article
Biochemistry & Molecular Biology
Sabine A. F. Jegouzo, Edward C. Harding, Oliver Acton, Maximus J. Rex, Andrew J. Fadden, Maureen E. Taylor, Kurt Drickamer
Article
Biochemistry & Molecular Biology
Hadar Feinberg, Sabine A. F. Jegouzo, Thomas J. W. Rowntree, Yue Guan, Matthew A. Brash, Maureen E. Taylor, William I. Weis, Kurt Drickamer
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Biochemistry & Molecular Biology
Hadar Feinberg, Sabine A. F. Jegouzo, Yi Lasanajak, David F. Smith, Kurt Drickamer, William Weis, Maureen E. Taylor
Summary: The human mannose receptor expressed on macrophages and hepatic endothelial cells plays an important role in reducing tissue damage by scavenging released lysosomal enzymes, glycopeptide fragments of collagen, and pathogenic microorganisms. By binding to mannose, fucose, or N-acetylglucosamine (GlcNAc) residues on these targets, the receptor interacts with sugars in a Ca2+-dependent manner through its C-type carbohydrate-recognition domain 4 (CRD4), which has been investigated through glycan array screening and multiple crystal structures. The interactions with monosaccharides and oligosaccharide substructures present in these ligands provide insight into the mechanisms of binding and potential classes of ligands for the mannose receptor.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Maureen E. Taylor, Tom Snelling, David F. Smith, Kurt Drickamer
Article
Biochemistry & Molecular Biology
Jong-won Kim, James Budzak, Yu Liu, Sabine A. F. Jegouzo, Kurt Drickamer, Maureen E. Taylor
Meeting Abstract
Biochemistry & Molecular Biology
Maureen Taylor, Hadar Feinberg, Thomas Rowntree, Shawn Tan, William Weis, Kurt Drickamer