Article
Multidisciplinary Sciences
Yu Jin Jang, Mijeong Kim, Bum-Kyu Lee, Jonghwan Kim
Summary: The authors developed a simple procedure to convert human primed pluripotent stem cells into trophoblast stem-like cells using bone morphogenetic protein 4, providing an important opportunity to study human placenta development and pathology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Bing-Chun Liu, Fang-Yuan Liu, Xin-Yue Gao, Yang-Lin Chen, Qiao-Qiao Meng, Yong-Li Song, Xi-He Li, Si-Qin Bao
Summary: The study reveals that Wnt signaling plays a crucial role in driving human pluripotent stem cells towards neural stem cells differentiation and maintaining their self-renewal. CLNSCs exhibit spinal cord properties and high proliferative capacity, providing a new cell model for investigating neurogenesis mechanisms and potential applications in regenerative medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Zhuoran Yu, Sihan Zhou, Minli Liu, Lijun Chen, Xue Zhang, Xu Yang, Hongdi Cui, Chaoqian Jiang, Yanjiao Lv, Tingsheng Yan, Qingran Kong, Zhonghua Liu
Summary: This study successfully derived and maintained stable porcine pluripotent stem cells (pLCDMs) using the LCDM culture system. Transcriptome analysis revealed the unique molecular characteristics and similarities/differences with human and mouse EPSCs. Furthermore, two small molecules in the culture system were found to play a crucial role in promoting trophoblast lineage differentiation potential of pLCDMs.
Article
Cell Biology
Glyn Nigel Stacey, Jie Hao
Summary: In recent years, significant progress has been made in China in establishing hPSCs for the manufacture of cell therapies. The National Stem Cell Resource Center and its partner organizations have over 600 hPSC lines formally recorded, with 47 of them also registered for European Commission funded research projects. This national and international coordination aims to promote the translation of Chinese hPSC-based products into clinical use according to established standards.
CELL PROLIFERATION
(2022)
Article
Cell Biology
Taiki Satoh, Marcelo A. S. Toledo, Janik Boehnke, Kathrin Olschok, Niclas Flosdorf, Katrin Goetz, Caroline Kuestermann, Stephanie Sontag, Kristin Sere, Steffen Koschmieder, Tim H. Bruemmendorf, Nicolas Chatain, Yoh-ichi Tagawa, Martin Zenke
Summary: Dendritic cells (DC) are professional antigen-presenting cells that can be divided into different subsets, with the DC3 subset having proinflammatory properties. Human iPS cell-derived DC3 exhibit similar characteristics to blood DC3 in vitro. The JAK2 V617F mutation enhances DC3 production and biases towards erythrocytes and megakaryocytes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Shin-Jeong Lee, Cholomi Jung, Jee Eun Oh, Sangsung Kim, Sangho Lee, Ji Yoon Lee, Young-sup Yoon
Summary: Red blood cell (RBC) transfusion is a lifesaving procedure for anemia and hemoglobin disorders, but faces challenges due to the shortage of blood supply and risks of infection and incompatibility. The use of human pluripotent stem cells (hPSCs), including human induced pluripotent stem cells (hiPSCs), holds promise for generating RBCs. This review discusses the concepts and mechanisms of erythropoiesis, methodologies for differentiating hPSCs into erythrocytes, and the limitations and future directions of using hiPSC-derived erythrocytes in clinical applications.
Article
Biotechnology & Applied Microbiology
Yunlong Tao, Xueyan Li, Qiping Dong, Linghai Kong, Andrew J. Petersen, Yuanwei Yan, Ke Xu, Seth Zima, Yanru Li, Danielle K. Schmidt, Melvin Ayala, Sakthikumar Mathivanan, Andre M. M. Sousa, Qiang Chang, Su-Chun Zhang
Summary: Researchers have successfully generated central norepinephrine (NE) neurons from stem cells, providing a tool for studying their roles in psychiatric and neurodegenerative diseases.
NATURE BIOTECHNOLOGY
(2023)
Article
Cell Biology
Kwaku Dad Abu-Bonsrah, Donald F. Newgreen, Mirella Dottori
Summary: This study successfully differentiated human embryonic stem cells into thyroid C cell-like cells, mimicking the process of medullary thyroid carcinoma in vitro, providing a valuable platform for drug screening.
Review
Medicine, General & Internal
Chang Liu, Xing Feng, Guoping Li, Priyanka Gokulnath, Junjie Xiao
Summary: Human pluripotent stem cell (hPSC) technology provides abundant opportunities for disease modeling. Cardiomyocytes derived from hPSCs are efficient tools for studying cardiac diseases, drug screening, and pathological mechanisms. This review discusses the advantages and limitations of the 2D hPSC-cardiomyocyte system and introduces recent developments in 3D culture platforms derived from hPSCs.
Article
Cell Biology
Guohui Bian, Yanzheng Gu, Changlu Xu, Wenyu Yang, Xu Pan, Yijin Chen, Mowen Lai, Ya Zhou, Yong Dong, Bin Mao, Qiongxiu Zhou, Bo Chen, Tatsutoshi Nakathata, Lihong Shi, Min Wu, Yonggang Zhang, Feng Ma
Summary: Research has shown that there are two types of mast cells in humans, one expressing tryptase and chymase found in the skin and connective tissues, and another type primarily expressing tryptase found in the mucosa. Through an efficient coculture system, a cell population of CD34(+)c-kit(+) cells was found to stimulate the production of MC(TC)s, which exhibit strong histamine release and immune response functions. This early developing tissue-type MC(TC)s may play a significant role in tumor immunity, with the release of IL-17 suggesting a central role in this immune response.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2021)
Article
Oncology
Kenichi Makino, Mark D. Long, Ryutaro Kajihara, Satoko Matsueda, Takaaki Oba, Kazunori Kanehira, Song Liu, Fumito Ito
Summary: This study demonstrates the critical role of Notch signaling in regulating the developmental pathway of human cDCs, providing insights into the future development of personalized treatment with unlimited numbers of autologous cDCs from human iPSCs.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Biochemistry & Molecular Biology
Goekhan Yuecel, Xiaobo Zhou, Linda Terkatz, Angela Wendel, Julius Reinhardt, Ibrahim El-Battrawy, Katherine Sattler, Lukas Cyganek, Jochen Utikal, Harald Langer, Ruediger Scharf, Daniel Duerschmied, Ibrahim Akin
Summary: PAMPs play a role in the pathogenesis of septic cardiomyopathy through TLR-mediated immune response. hiPSC-CMs provide a useful tool for studying PAMP-induced changes in cardiomyocytes. Higher doses of flagellin increase the expression levels of inflammation-associated cytokines and downstream signaling molecules, as well as the expression and fluorescence proportion of TLR5 in hiPSC-CMs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Youkun Bi, Xinlong Qiao, Qun Liu, Shaole Song, Keqi Zhu, Xun Qiu, Xiang Zhang, Ce Jia, Huiwen Wang, Zhiguang Yang, Ying Zhang, Guangju Ji
Summary: This study identified the protein and miRNA profiles of exosomes derived from hESCs, hiPSCs, and hUC-MSCs. Bioinformatics analysis and experimental validation revealed the diverse biological functions of these exosomes and their potential applications in different fields. These findings are important for optimizing exosome selection in preclinical and clinical trials.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Biochemistry & Molecular Biology
Rowan M. Karvas, Laurent David, Thorold W. Theunissen
Summary: Trophoblasts play critical roles in blastocyst implantation and maternal-fetal communication during pregnancy. However, our understanding of human trophoblast biology is limited due to the scarcity of first-trimester placental tissue and the inadequacy of animal models. Recent advancements in deriving human trophoblast stem cells from pluripotent and somatic cell sources provide exciting opportunities to study the molecular mechanisms of placental development and pregnancy-related diseases.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biology
Wai Hoe Ng, Elizabeth K. Johnston, Jun Jie Tan, Jacqueline M. Bliley, Adam W. Feinberg, Donna B. Stolz, Ming Sun, Piyumi Wijesekara, Finn Hawkins, Darrell N. Kotton, Xi Ren
Summary: The interaction between the developing heart and lung is crucial for their proper formation. Researchers have developed a new human model using pluripotent stem cells to investigate this relationship, creating microtissues containing both heart and lung cells. The presence of developing heart cells was found to accelerate lung maturation in the microtissues, and the heart and lung tissues were observed to automatically separate from each other during later stages.
Article
Cell & Tissue Engineering
Mathew G. Angelos, Juan E. Abrahante, Robert H. Blum, Dan S. Kaufman
Article
Cell & Tissue Engineering
Ye Li, David L. Hermanson, Branden S. Moriarity, Dan S. Kaufman
Review
Hematology
Mathew G. Angelos, Dan S. Kaufman
CURRENT OPINION IN HEMATOLOGY
(2018)
Review
Immunology
Michelle L. Saetersmoen, Quirin Hammer, Bahram Valamehr, Dan S. Kaufman, Karl-Johan Malmberg
SEMINARS IN IMMUNOPATHOLOGY
(2019)
Review
Clinical Neurology
Anastasie M. Dunn-Pirio, Benjamin M. Heyman, Dan S. Kaufman, Revere P. Kinkel
CURRENT TREATMENT OPTIONS IN NEUROLOGY
(2019)
Article
Hematology
Huang Zhu, Robert H. Blum, Ryan Bjordahl, Svetlana Gaidarova, Paul Rogers, Tom Tong Lee, Ramzey Abujarour, Gregory B. Bonello, Jianming Wu, Pei-Fang Tsai, Jeffrey S. Miller, Bruce Walcheck, Bahram Valamehr, Dan S. Kaufman
Article
Cell & Tissue Engineering
Fahad Kidwai, Byron W. H. Mui, Deepika Arora, Kulsum Iqbal, Madison Hockaday, Luis Fernandez de Castro Diaz, Natasha Cherman, Daniel Martin, Vamsee D. Myneni, Moaz Ahmad, Katarzyna Futrega, Sania Ali, Randall K. Merling, Dan S. Kaufman, Janice Lee, Pamela G. Robey
Article
Cell & Tissue Engineering
Huang Zhu, Robert H. Blum, Davide Bernareggi, Eivind Heggernes Ask, Zhengming Wu, Hanna Julie Hoel, Zhipeng Meng, Chengsheng Wu, Kun-Liang Guan, Karl-Johan Malmberg, Dan S. Kaufman
Article
Immunology
Benjamin H. Goldenson, Huang Zhu, YunZu Michele Wang, Naveen Heragu, Davide Bernareggi, Alessa Ruiz-Cisneros, Andres Bahena, Eivind Heggernes Ask, Hanna Julie Hoel, Karl-Johan Malmberg, Dan S. Kaufman
FRONTIERS IN IMMUNOLOGY
(2020)
Review
Biochemistry & Molecular Biology
William C. Hahn, Joel S. Bader, Theodore P. Braun, Andrea Califano, Paul A. Clemons, Brian J. Druker, Andrew J. Ewald, Haian Fu, Subhashini Jagu, Christopher J. Kemp, William Kim, Calvin J. Kuo, Michael McManus, Gordon B. Mills, Xiulei Mo, Nidhi Sahni, Stuart L. Schreiber, Jessica A. Talamas, Pablo Tamayo, Jeffrey W. Tyner, Bridget K. Wagner, William A. Weiss, Daniela S. Gerhard
Summary: Characterization of cancer genomes has provided insight into somatically altered genes across tumors, but the function of most cancer alleles remains mysterious and does not impact therapy for most patients. Approaches to understand the function and circuitry of cancer genes provide complementary approaches to elucidate both oncogene and non-oncogene dependencies. Emerging work indicates that therapeutic targets engendered by non-oncogene dependencies are more diverse than recurrently mutated genes, providing new opportunities for clinical translation.
Editorial Material
Cell & Tissue Engineering
Benjamin H. Goldenson, Dan S. Kaufman
Summary: The study demonstrates that expression of modified CD16a and IL-15 receptor combined with knockout of CD38 can enhance NK cell-mediated activity against leukemia and multiple myeloma.
Article
Allergy
Lori Broderick, Gwendolyn M. Clay, Robert H. Blum, Yang Liu, Rachael McVicar, Fabio Papes, Laela M. Booshehri, Ian G. Cowell, Caroline A. Austin, Christopher D. Putnam, Dan S. Kaufman
Summary: The study examines the impact of Hoffman syndrome-associated mutations in TOP2B on the development and function of NK cells. Using a knockin murine model and patient-derived iPSCs, the researchers investigated NK-cell development in mouse bone marrow and spleen, and assessed the immunophenotype, gene expression, and cytotoxic activity of NK cells in both murine and human models.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Cell & Tissue Engineering
Somayeh Pouyanfard, Nairika Meshgin, Luisjesus S. Cruz, Karin Diggle, Hamidreza Hashemi, Timothy Pham, Manuel Fierro, Pablo Tamayo, Andrea Fanjul, Tatiana Kisseleva, Dan S. Kaufman
Summary: The study shows that human iPSC-derived macrophages can effectively reduce gene expression and pathological markers of liver fibrosis, offering a promising cell therapy approach to ameliorate fibrosis.
Article
Cell Biology
Frank Cichocki, Ryan Bjordahl, Svetlana Gaidarova, Sajid Mahmood, Ramzey Abujarour, Hongbo Wang, Katie Tuininga, Martin Felices, Zachary B. Davis, Laura Bendzick, Raedun Clarke, Laurel Stokely, Paul Rogers, Moyar Ge, Megan Robinson, Betsy Rezner, David L. Robbins, Tom T. Lee, Dan S. Kaufman, Bruce R. Blazar, Bahram Valamehr, Jeffrey S. Miller
SCIENCE TRANSLATIONAL MEDICINE
(2020)
