Article
Biochemistry & Molecular Biology
Bertille Montibus, Jil Cercy, Tristan Bouschet, Amandine Charras, Stephanie Maupetit-Mehouas, David Nury, Celine Gonthier-Gueret, Sabine Chauveau, Nicolas Allegre, Caroline Chariau, Charles C. Hong, Isabelle Vaillant, C. Joana Marques, Franck Court, Philippe Arnaud
Summary: The induction of Kdm6b expression upon neural commitment of mouse embryonic stem cells is linked to rearrangement between two 3D configurations defined by the promoter contact with different regions in the Kdm6b locus. This rearrangement is associated with changes in 5-hydroxymethylcytosine (5hmC) levels and requires the functional ten-eleven-translocation (TET) 3 protein. This reveals an unexpected interplay between the 5-hmC and H3K27me3 pathways during neural lineage commitment in mammals.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Gastroenterology & Hepatology
Hannah M. Kolev, Avital Swisa, Elisabetta Manduchi, Yemin Lan, Rachel R. Stine, Giuseppe Testa, Klaus H. Kaestner
Summary: This study investigated the role of H3K27me3 demethylation in the intestine and found that the demethylases KDM6A/B are required for the full transcriptomic and epigenomic landscape of the intestinal epithelium and the expression of key Paneth cell genes.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2023)
Article
Cell Biology
Mohammed Razeeth Shait Mohammed, Mazin Zamzami, Hani Choudhry, Firoz Ahmed, Bushra Ateeq, Mohammad Imran Khan
Summary: This study discovered that H3K27 histone demethylases, KDM6A/B, are highly expressed during ECM detachment. Inhibition of KDM6A/B activity reduces sphere formation capacity and increases apoptosis. KDM6B maintains stemness by transcriptionally regulating the expression of stemness genes and plays a regulatory role in maintaining hypoxia in detached cancer cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Xiao-jiao-yang Li, Fei Zhou, Ya-jing Li, Xiao-yong Xue, Jiao-rong Qu, Gui-fang Fan, Jia Liu, Rong Sun, Jian-zhi Wu, Qi Zheng, Run-ping Liu
Summary: Liver fibrosis is a wound-healing process characterized by excess formation of extracellular matrix (ECM) from activated hepatic stellate cells (HSCs). Previous studies show that EZH2 and long non-coding RNA H19 are highly correlated with fibrogenesis. In the current study, the mechanisms underlying this correlation were investigated, and it was found that EZH2 was upregulated and correlated with H19 and fibrotic markers expression in liver fibrosis models. The administration of EZH2 inhibitor 3-DZNeP showed protective effects in these models.
ACTA PHARMACOLOGICA SINICA
(2023)
Article
Oncology
[Anonymous]
Summary: Immunosuppressive myeloid cells, characterized by high expression of the epigenetic enzyme KDM6B, are associated with resistance to anti-PD1 therapy in patients with glioblastoma. However, deleting or inhibiting KDM6B reprograms these myeloid cells into an immunostimulatory phenotype, thus overcoming resistance to anti-PD1 therapy in preclinical models of glioblastoma.
Article
Cell Biology
Zhi Cao, Xiaolei Shi, Feng Tian, Yu Fang, Jason Boyang Wu, Stefan Mrdenovic, Xinwen Nian, Jin Ji, Huan Xu, Chen Kong, Yalong Xu, Xi Chen, Yuhua Huang, Xuedong Wei, Yongwei Yu, Bo Yang, Leland W. K. Chung, Fubo Wang
Summary: The study revealed that KDM6B is overexpressed in prostate cancer, with elevated expression associated with high Gleason Score, low serum PSA level, and shorter recurrence-free survival. KDM6B promotes proliferation, migration, invasion, and cell cycle progression, while suppressing apoptosis in prostate cancer cells. The KDM6B inhibitor GSK-J4 can effectively suppress the biological functions of KDM6B in prostate cancer cells.
CELL DEATH & DISEASE
(2021)
Article
Dentistry, Oral Surgery & Medicine
Hao Jiang, Peizeng Jia
Summary: The study revealed that miR-153-3p inhibits PDLSC osteogenesis by targeting KDM6A and suppressing the transcription of ALP, Runx2, and OPN, providing potential hope for the application of PDLSCs in periodontal therapy.
JOURNAL OF PERIODONTAL RESEARCH
(2021)
Article
Cell & Tissue Engineering
Dorota Lubanska, Ingrid Qemo, Megan Byrne, Kaitlyn N. Matthews, Bre-Anne Fifield, Jillian Brown, Elizabeth Fidalgo da Silva, Lisa A. Porter
Summary: Fully differentiated cells can be reprogrammed through ectopic expression of key transcription factors to create induced pluripotent stem cells, which have great potential in disease modeling and regenerative medicine. However, this process has limitations due to the upregulation of antiproliferative genes, resulting in low efficiency. Our data reveals a unique cell cycle mechanism where mouse fibroblasts are able to repress cyclin dependent kinase inhibitors through the activation of the epigenetic regulator EZH2 by a cyclin-like protein SPY1, thus increasing reprogramming efficiency.
Article
Developmental Biology
Junghyun Kim, Yogendra Bordiya, Yanpeng Xi, Bo Zhao, Dong-Hwan Kim, Youngjae Pyo, Wei Zong, William A. Ricci, Sibum Sung
Summary: Changes in ambient temperature greatly affect developmental programs in many species. Plants adapt to high ambient growth temperature through the involvement of VIL1, which leads to genome-wide accumulation of H3K27me3. VIL1 is necessary for thermo-morphogenesis and loss of function results in hypo-responsiveness to warm ambient temperature.
Article
Plant Sciences
Beibei Zheng, Jingjing Liu, Anqi Gao, Xiaomei Chen, Lingling Gao, Liao Liao, Binwen Luo, Collins Otieno Ogutu, Yuepeng Han
Summary: In this study, the authors investigated the DNA methylation and H3K27me3 modification during the leaf-to-callus transition in peach. They found that DNA hypomethylation and H3K27me3 demethylation occurred during in vitro culture of leaf explants, which was accompanied by callus development. The DNA methylation inhibitor 5-azacytidine increased callus development, while the H3K27me3 demethylase inhibitor GSK-J4 reduced callus development. Overall, this study provides new insights into the epigenetic mechanisms regulating callus development.
HORTICULTURE RESEARCH
(2022)
Article
Cell Biology
Qiting He, Jie Shi, Wei Liu, Wei Zhao, Zihao Wang, Kaiwen Liu, Dawang Zhao, Shaoyi Wang, Yongyuan Guo, Lei Cheng, Yuan Gao
Summary: This study found that epigenetic mechanisms play an important role in the migration of mesenchymal stem cells and that H3K27me3 is at least partially involved in the migration of mesenchymal stem cells induced by TGF-beta 1.
CELL DEATH DISCOVERY
(2022)
Article
Biochemistry & Molecular Biology
Jyotirmaya Behera, Jessica Ison, Hitesh Rai, Neetu Tyagi
Summary: The study demonstrates that allyl sulfide (AS) can promote differentiation and mineralization of bone marrow-derived mesenchymal stem cells (BMMSCs) and increase bone density by inhibiting mitochondrial oxidative damage. Furthermore, AS restores mitochondrial redox homeostasis, inhibits mtDNA release, and enhances osteogenesis and bone density through an epigenetic mechanism. The findings suggest that AS could be a potential drug target for aging-associated osteoporosis treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Cell & Tissue Engineering
Miao Zhang, Xin Zhao, Xiao Feng, Xiao Hu, Xuan Zhao, Wange Lu, Xinyi Lu
Summary: This study reveals the distinct roles of HIRA complex members in controlling retrotransposons and cell fate conversion in embryonic stem cells.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Plant Sciences
Lauriane Simon, Aline V. Probst
Summary: Controlled transcription of genes is crucial for cell differentiation and development. This process involves multiple layers of regulation, including nucleosome composition, chromatin folding, and chromatin interactions in the nucleus. Recent technological advances have provided insight into these mechanisms and dynamic changes during stress response and development.
Article
Cell Biology
Sheila Q. Xie, Bryony J. Leeke, Chad Whilding, Ryan T. Wagner, Ferran Garcia-Llagostera, YiXuan Low, Paul Chammas, Nathan T-F Cheung, Dirk Dormann, Michael T. McManus, Michelle Percharde
Summary: Remarkably, our study reveals a close link between rRNA synthesis, nucleolar maturation, and gene repression during early development.
GENES & DEVELOPMENT
(2022)
Article
Endocrinology & Metabolism
Lei Yang, Xuefei Liu, Lishuang Song, Guanghua Su, Anqi Di, Chunling Bai, Zhuying Wei, Guangpeng Li
JOURNAL OF PINEAL RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Lei Yang, Xuefei Liu, Lishuang Song, Anqi Di, Guanghua Su, Chunling Bai, Zhuying Wei, Guangpeng Li
Article
Reproductive Biology
Guanghua Su, Shanshan Wu, Meiling Wu, Lina Wang, Lei Yang, Mengxin Du, Xiaoyu Zhao, Xiaohu Su, Xuefei Liu, Chunling Bai, Zhuying Wei, Lei Cheng, Guangpeng Li
Summary: The study showed that melatonin has a significant impact on the entire process of sperm cryopreservation, oocyte maturation, and embryonic development. Melatonin can improve sperm viability, membrane integrity, reduce ROS levels, increase oocyte maturation rate, and enhance total cell numbers of IVF blastocysts. Melatonin treatment also influences the expression levels of key regulatory genes associated with embryo genome activation.
Article
Biochemistry & Molecular Biology
Guanghua Su, Lina Wang, Guangqi Gao, Shanshan Wu, Lei Yang, Meiling Wu, Xuefei Liu, Miaomiao Yang, Zhuying Wei, Chunling Bai, Guangpeng Li
Summary: In interspecies somatic cell nuclear transfer (iSCNT) embryos, a developmental block in the 8-cell stage was identified, characterized by lower expression of ribosomal subunit genes and abnormal nucleolin structure. Modulating the expression of nucleolin protein (C23) improved blastocyst development in both SCNT and iSCNT embryos, suggesting that abnormal C23 and ribosomal subunit gene expression leads to developmental block and ZGA failure in iSCNT embryos.
Article
Agriculture, Dairy & Animal Science
Di Wu, Mingjuan Gu, Zhuying Wei, Chunling Bai, Guanghua Su, Xuefei Liu, Yuefang Zhao, Lei Yang, Guangpeng Li
Summary: MSTN knockout regulates liver metabolism and promotes bile acid metabolism. This is achieved through enhanced bile acid synthesis, which in turn increases lean muscle mass.
Article
Cell Biology
Mingjuan Gu, Xinyu Zhou, Lin Zhu, Yajie Gao, Li Gao, Chunling Bai, Lei Yang, Guangpeng Li
Summary: The study found that Myostatin mutation had no effect on cattle cardiac muscle histologically, but increased glycolysis in biochemical assays. Phosphorylation of PFK was potentiated through the PDE5A-cGMP-PKG pathway, promoting glycolysis in the heart.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Lin Zhu, Xueqiao Wang, Zhuying Wei, Miaomiao Yang, Xinyu Zhou, Jiaru Lei, Chunling Bai, Guanghua Su, Xuefei Liu, Lei Yang, Guangpeng Li
Summary: This study investigated the effect of reducing myostatin (MSTN) expression on muscle antioxidant capacity. The results showed that reducing MSTN expression enhances the antioxidant capacity of muscles by regulating the activity of AMPK and G6PD enzyme.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Microbiology
Xinyu Zhou, Mingjuan Gu, Lin Zhu, Di Wu, Miaomiao Yang, Yajie Gao, Xueqiao Wang, Chunling Bai, Zhuying Wei, Lei Yang, Guangpeng Li
Summary: Myostatin gene-edited cattle showed similar microbial diversity and dominant bacteria in the four stomach compartments compared to wild-type cattle, but with significant differences in microbiota composition. Metabolomics analysis revealed differences in certain metabolites between the two types of cattle in the rumen, reticulum, and abomasum, with differences in metabolic pathways. Spearman correlation network analysis demonstrated a significant correlation between microflora composition and metabolic pathways.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Lin Zhu, Chunling Bai, Xueqiao Wang, Zhuying Wei, Mingjuan Gu, Xinyu Zhou, Guanghua Su, Xuefei Liu, Lei Yang, Guangpeng Li
Summary: The knockdown of MSTN accelerates the pentose phosphate pathway, thereby enhancing the antioxidant capacity of erythrocytes. These findings provide important insights into the role of MSTN in erythrocyte antioxidant regulation after exhaustive exercise.
Article
Biochemistry & Molecular Biology
Xueqiao Wang, Lin Zhu, Zhuying Wei, Mingjuan Gu, Miaomiao Yang, Xinyu Zhou, Chunling Bai, Guanghua Su, Xuefei Liu, Lei Yang, Guangpeng Li
Summary: This study investigated the effects of exogenous fat-1 transgene on energy metabolism and its relationship with DNA methylation. The results showed that exogenous fat-1 transgene significantly increased n-3 PUFA content, while decreasing n-6 PUFA content and the n-6: n-3 PUFA ratio. Furthermore, the increase in exogenous fat-1 transgene reduced ATP synthesis and the activity and expression of key rate-limiting enzymes in glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation. DNA methylation sequencing indicated that the inhibition of gene expression may be due to altered DNA methylation. Thus, altering the balance of n-6/n-3 PUFA could regulate DNA methylation, which in turn affects mitochondrial energy metabolism.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biology
Yuefang Zhao, Lei Yang, Guanghua Su, Zhuying Wei, Xuefei Liu, Lishuang Song, Chao Hai, Di Wu, Zhenting Hao, Yunxi Wu, Li Zhang, Chunling Bai, Guangpeng Li
Summary: In this study, the myostatin gene (MSTN) in Chinese Yellow Cattle was knocked out using CRISPR/Cas9 technology, resulting in improved growth traits and normal fertility in the gene-edited cattle. These findings suggest that gene-edited Chinese Yellow Cattle can be used for beef production and breeding.
Article
Biochemistry & Molecular Biology
Shanshan Wu, Xiaoyu Zhao, Meiling Wu, Lei Yang, Xuefei Liu, Danyi Li, Han Xu, Yuefang Zhao, Xiaohu Su, Zhuying Wei, Chunling Bai, Guanghua Su, Guangpeng Li
Summary: In this study, using inter-species somatic cell nuclear transfer (iSCNT) as a model, the researchers found that ovine-bovine cloned embryos were developmentally blocked at the 8-cell stage and exhibited abnormal mitochondrial function and gene expression. Further investigation revealed that the overexpression and knockdown of the mitochondrial fusion gene Mfn1 significantly affected mitochondrial fusion, as well as embryonic development and gene expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xueqiao Wang, Zhuying Wei, Mingjuan Gu, Lin Zhu, Chao Hai, Anqi Di, Di Wu, Chunling Bai, Guanghua Su, Xuefei Liu, Lei Yang, Guangpeng Li
Summary: Myostatin (MSTN) is a negative regulator of skeletal muscle growth and plays an important role in regulating mitochondrial energy metabolism in mice. Loss of MSTN inhibits oxidative phosphorylation and impairs ATP production in skeletal muscles. This inhibition may be mediated through the TGF-beta-Smad2/3 pathway. These findings demonstrate that MSTN is a crucial regulator of mitochondrial energy homeostasis in mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mingjuan Gu, Song Wang, Anqi Di, Di Wu, Chao Hai, Xuefei Liu, Chunling Bai, Guanghua Su, Lei Yang, Guangpeng Li
Summary: In this study, the metabolic and transcriptional profiling in esophageal smooth muscles of MSTN-KO Chinese Luxi Yellow cattle were investigated using metabolome and transcriptome analyses. Significantly differentially expressed genes and metabolites were identified, with enrichment in multiple signaling pathways and metabolic pathways. These findings provide important references for further understanding the effect of MSTN knockout on smooth muscle.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Chao Hai, Chunling Bai, Lei Yang, Zhuying Wei, Hong Wang, Haoran Ma, Haibing Ma, Yuefang Zhao, Guanghua Su, Guangpeng Li
Summary: The utilization of MSTN gene-edited Luxi bulls in breeding can improve the growth and performance of hybrid cattle, providing potential benefits for both farmers and consumers.