Article
Pharmacology & Pharmacy
Huan Yang, Wanda van der Stel, Randy Lee, Caroline Bauch, Sam Bevan, Paul Walker, Bob van de Water, Erik H. J. Danen, Joost B. Beltman
Summary: Mitochondria are the main bioenergetic organelles of cells and exposure to chemicals targeting mitochondria generally results in toxicity. Different mitochondrial respiration inhibitors have varying effects on mitochondrial membrane potential, and mathematical modeling can help understand these dynamics. Additionally, incorporating pharmacokinetic decay into the model can improve the fit for certain compounds, showing a complex interplay between pharmacokinetics and MMP dynamics within mitochondria.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Environmental Sciences
Xiangping Tan, Yanxia Nie, Xiaomin Ma, Zhiming Guo, Yang Liu, Haixia Tian, Mallavarapu Megharaj, Weijun Shen, Wenxiang He
Summary: The study showed that high fertility soils had higher organic carbon, nutrient content, microbial activity, and enzyme kinetics parameters compared to low fertility soils. Soil chemical properties primarily influenced the values of V-max and K-m of enzymes, while the abundance of main microbial groups and fungi/bacteria ratio had a minor effect on them. Soil fertility and microbial communities interactively explained a portion of the variance in Km values.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Food Science & Technology
Chunyun Ma, Yan Zhang, Chuankai Yang, Yuhang Zhang, Miaorong Zhang, Jianguo Tang
Summary: Cetyl trimethyl ammonium bromide (CTAB)-activated lipase-mineral hybrid microflowers (CTAB-Lip-MHFs) showed good substrate affinity and stability for fatty acid production through hydrolysis of olive oil.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Cristina Algieri, Fabiana Trombetti, Alessandra Pagliarani, Micaela Fabbri, Salvatore Nesci
Summary: The study found that gadolinium has a strong inhibitory effect on both the mitochondrial Ca2+-activated F1FO-ATPase and the PTP, strengthening the link between the two. This provides new insights into the biological effects of Gd3+.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Olivier Lurette, Hala Guedouari, Jordan L. Morris, Rebeca Martin-Jimenez, Julie-Pier Robichaud, Genevieve Hamel-Cote, Mehtab Khan, Nicholas Dauphinee, Nicolas Pichaud, Julien Prudent, Etienne Hebert-Chatelain
Summary: The architecture of mitochondria is regulated by the tyrosine kinase Src, which affects mitochondrial morphology independently of mitochondrial size, cellular respiration, or ATP levels. This study highlights a novel function for Src in the control of mitochondrial dynamics.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Masami Nakazawa, Mutsuki Takahashi, Ryuta Hayashi, Yuki Matsubara, Yuichiro Kashiyama, Mitsuhiro Ueda, Hiroshi Inui, Tatsuji Sakamoto
Summary: This study revealed the crucial role of NNT in ATP production and wax ester formation in Euglena gracilis during anaerobic metabolism, with silencing of NNT genes leading to significant decreases in these processes. Additionally, a similar phenotype was observed when the gene encoding mitochondrial NADP(+)dependent malic enzyme was silenced.
Article
Multidisciplinary Sciences
Harpreet Shinhmar, Chris Hogg, Glen Jeffery
Summary: Aged mitochondrial function can be improved with long wavelength light exposure. This study investigated the impact of 670nm light on cytokine expression in blood serum and retina in mice. The results showed that the light exposure shifted patterns of cytokine expression, selectively increasing some interleukins and chemokines. The findings suggest that long wavelengths can have systemic effects and further analysis is needed to understand the implications of cytokine upregulation by light.
Article
Biochemistry & Molecular Biology
Iva Kladnicka, Miroslava Cedikova, Jan Jedlicka, Michaela Kohoutova, Ludek Muller, Iveta Plavinova, Michaela Kripnerova, Monika Bludovska, Jitka Kuncova, Dana Mullerova
Summary: This study evaluated the impact of exposure to p,p'-DDE on mitochondrial function in adipocytes, finding that low concentrations of p,p'-DDE increased basal mitochondrial respiration and ATP-linked oxygen consumption, while higher concentrations slowed down the normal increase in oxygen consumption during adipogenesis.
Article
Chemistry, Analytical
Charles Blanluet, Diego A. Huyke, Ashwin Ramachandran, Alexandre S. Avaro, Juan G. Santiago
Summary: This study demonstrates that endpoint-based assays in CRISPR-Cas12 detection are limited by experimental choices and work best for known target concentrations. It also reveals that SNP activation results in measurable changes in the kinetic parameters of Cas12 trans-cleavage. To overcome the limitations of endpoint-based assays, the researchers developed a new assay based on the quantification of Michaelis-Menten parameters.
ANALYTICAL CHEMISTRY
(2022)
Article
Energy & Fuels
Yong-Rui Pi, Mu -Tai Bao
Summary: Enzymes and characteristics of petroleum-degrading bacteria are crucial for crude oil biodegradation. The study reveals that Proteobacteria, Firmcutes, and other bacterial groups play a significant role in petroleum hydrocarbon degradation. Acinetobacter shows a strong correlation with the degradation of n-alkanes and PAHs. Additionally, functional categories related to carbohydrate and amino acid metabolism, xenobiotic biodegradation, and membrane transport are important in bacterial communities.
Article
Biochemistry & Molecular Biology
Yu Sun, Xiujuan Wei, Fang Fang, Yiping Shen, Haiyan Wei, Jiuwei Li, Xianglai Ye, Yongkun Zhan, Xiantao Ye, Xiaomin Liu, Wei Yang, Yuhua Li, Xiangju Geng, Xuelin Huang, Yiyan Ruan, Zailong Qin, Shang Yi, Jianxin Lyu, Hezhi Fang, Yongguo Yu
Summary: Mitochondrial diseases caused by variants in HPDL gene can result in impaired mitochondrial respiratory function and dysfunction of OXPHOS complex II. The study found that bi-allelic inactivations of HPDL lead to neurodegenerative disease characterized by pediatric-onset spastic movement phenotypes, expanding the understanding of mitochondrial neuropathy mechanisms.
JOURNAL OF GENETICS AND GENOMICS
(2021)
Article
Food Science & Technology
Dayoung Kim, Eun Ko, Moonsung Choi, Sooim Shin
Summary: The study determined the kinetic parameters of mitochondrial complexes I-IV, and analyzed the catalytic activity and binding affinity between substrates and enzymes. Results showed that the kinetic parameters varied among different complexes, reflecting the distinct characteristics of the enzymes.
APPLIED BIOLOGICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Aurelie Monie, Thomas Habersetzer, Lea Sureau, Annabelle David, Karine Clemens, Myriam Malet-Martino, Emile Perez, Sophie Franceschi, Stephance Balaysssac, Mathieu Delample
Summary: We investigated the use of rapeseed oil as the main oil in ice cream formulations by modifying its functionality with different lipases. The lipolysis process was assessed by 13C NMR, which showed that higher levels of free fatty acids resulted in faster crystallization and lower melting temperatures. These modifications had a significant impact on the overall hardness and flow of the ice cream during defrosting. The composition of low-molecular polar lipids within the oil played a key role in controlling the overall behavior of the ice cream products.
FOOD RESEARCH INTERNATIONAL
(2023)
Review
Biochemistry & Molecular Biology
Marina Barba-Aliaga, Paula Alepuz
Summary: The eukaryotic translation initiation factor 5A (eIF5A) plays a crucial role in translation and cellular processes. It has been associated with various diseases and proposed as an anti-aging factor. Recent research has revealed its important function in maintaining healthy mitochondria, although the precise mechanisms are still unclear. This review discusses the link between eIF5A and mitochondria, speculates on its role in regulating mitochondrial homeostasis, and highlights its potential as a target in diseases related to energy metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Soil Science
Kendalynn A. Morris, Andreas Richter, Mirco Migliavacca, Marion Schrumpf
Summary: This study investigated the impact of nitrogen and phosphorus fertilization on soil microbial growth, finding that soils with higher organic matter content may have higher microbial growth rates, and short-term nutrient imbalances may reduce microbial growth.
SOIL BIOLOGY & BIOCHEMISTRY
(2022)
Article
Biology
Martin Grosell, Rachael M. Heuer, N. C. Wu, Rebecca L. Cramp, Yadong Wang, Edward M. Mager, Ross G. Dwyer, Craig E. Franklin
JOURNAL OF EXPERIMENTAL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
A. Reynolds Kirby, Gina Galli, Janna Crossley, Lauren E. Sweet, Dane A. Crossley, Aaron P. Roberts
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2020)
Article
Physiology
Amanda Reynolds Kirby, Dane A. Crossley, Edward M. Mager
JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY
(2020)
Article
Environmental Sciences
Maria C. Cartolano, Igal Berenshtein, Rachael M. Heuer, Christina Pasparakis, Mitchell Rider, Neil Hammerschlag, Claire B. Paris, Martin Grosell, M. Danielle McDonald
ENVIRONMENTAL POLLUTION
(2020)
Article
Engineering, Environmental
Erik J. Folkerts, Rachael M. Heuer, Shannon Flynn, John D. Stieglitz, Daniel D. Benetti, Daniel S. Alessi, Greg G. Goss, Martin Grosell
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2020)
Article
Physiology
Zongli Yao, Kevin L. Schauer, Ilan M. Ruhr, Edward M. Mager, Rachael M. Heuer, Martin Grosell
Summary: The study demonstrates that exposure of toadfish to hypersaline seawater results in transient acidosis, which is compensated by increased branchial acid excretion, leading to full recovery within 48-96 hours. This compensation for intestinal base loss during hypersaline treatment appears to be through gill H(+) excretion rather than gill HCO(3)(-) uptake.
JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY
(2021)
Letter
Multidisciplinary Sciences
Philip L. Munday, Danielle L. Dixson, Megan J. Welch, Douglas P. Chivers, Paolo Domenici, Martin Grosell, Rachael M. Heuer, Geoffrey P. Jones, Mark I. McCormick, Mark Meekan, Goran E. Nilsson, Timothy Ravasi, Sue-Ann Watson
Article
Environmental Sciences
Lela S. Schlenker, Robin Faillettaz, John D. Stieglitz, Chi Hin Lam, Ronald H. Hoenig, Georgina K. Cox, Rachael M. Heuer, Christina Pasparakis, Daniel D. Benetti, Claire B. Paris, Martin Grosell
Summary: Identifying complex behaviors such as spawning and fine-scale activity in highly migratory fish species is challenging, but essential for fisheries management in a warming ocean. Using remotely transmitted acceleration data, researchers were able to predict spawning events and discovered drivers of high activity in mahi-mahi, showing that this information can be extracted from PSATs to study reproductive behavior and population connectivity in highly migratory fishes. This study highlights the necessity of unveiling fine-scale activity patterns to understand the ecology of highly mobile species.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Physiology
Nina G. Walker Hansen, Steffen S. Madsen, Melanie Brauckhoff, Rachael M. Heuer, Lela S. Schlenker, Morten B. Engelund, Martin Grosell
Summary: Gulf toadfish with aglomerular kidney can survive in various salinities including high Mg2+ environments. The study found that transcriptional regulation of renal Mg2+ transporters is influenced by a combination of elevated Mg2+ levels and salinity, rather than high Mg2+ levels alone.
JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY
(2021)
Article
Environmental Sciences
Rachael M. Heuer, John D. Stieglitz, Christina Pasparakis, Ian C. Enochs, Daniel D. Benetti, Martin Grosell
Summary: The study examined how temperature affects aerobic scope and swimming performance in mahi-mahi, finding that those acclimated to 28 degrees Celsius had the highest aerobic scope and critical swimming speed. Fish acclimated to 20 degrees Celsius experienced significantly decreased aerobic scope and critical swimming speed, while those at 32 degrees Celsius had increased mortality and decreased swimming performance. These results align with previously observed distribution patterns and have implications for climate change predictions and habitat utilization of wild mahi-mahi.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Jacelyn J. Shu, Rachael M. Heuer, Kelly D. Hannan, John D. Stieglitz, Daniel D. Benetti, Jodie L. Rummer, Martin Grosell, Colin J. Brauner
Summary: Teleost fishes have a unique O-2 transport system that may contribute to their success as a group. This study expanded the knowledge of this system to warm-water fish species, cobia and mahi-mahi, demonstrating the presence of a rapid, active red blood cell beta-NHE. Despite differences in phylogenetics and life history, there were few differences in the Hb-O-2 transport systems of these species, suggesting conservation of this physiological trait across diverse teleost taxa.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2022)
Article
Environmental Sciences
Christina Pasparakis, Yadong Wang, Rachael M. Heuer, Wenlong Zhang, John D. Stieglitz, Charles J. McGuigan, Daniel D. Benetti, Vernon P. Scholey, Daniel Margulies, Martin Grosell
Summary: Research has shown that pelagic fish embryos increase their specific gravity and lose buoyancy after exposure to ultraviolet radiation, a response that may be common to all fish with pelagic spawning strategies. Additionally, preliminary results suggest that early-life stage mahi-mahi may reduce further UV exposure by sensing external cues and modifying buoyancy.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Engineering, Environmental
Lela S. Schlenker, John D. Stieglitz, Justin B. Greer, Robin Faillettaz, Chi Hin Lam, Ronald H. Hoenig, Rachael M. Heuer, Charles J. McGuigan, Christina Pasparakis, Emma B. Esch, Gabrielle M. Menard, Alexandra L. Jaroszewski, Claire B. Paris, Daniel Schlenk, Daniel D. Benetti, Martin Grosell
Summary: This study investigates the impact of the 2010 Deepwater Horizon disaster on mahi-mahi. Laboratory experiments show that oil exposure affects the physiology and behavior of mahi-mahi, and this study provides evidence of long-term effects on wild populations. These findings offer valuable insights into the consequences of deep-sea oil spills.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Gabriela Maressa Machado Baesso, Amanda Vitoria Venancio, Livia Costa Varela Barca, Pepita Fernanda Peppi, Caique Aparecido Faria, Joao Pedro Cosmo Machado, Luiz David Solis Murgas, Alvaro Jose de Almeida Bicudo, Eduardo Mauricio Mendes de Lima, Andre Rodrigues da Cunha Barreto-Vianna
Summary: This study evaluated the effects of eugenol, menthol, and lidocaine as anesthetics on glucose levels and recovery times in zebrafish. The results showed that eugenol and menthol did not affect glucose levels, while lidocaine caused non-concentration-dependent hyperglycemia. Recovery times were similar for eugenol and menthol, but concentration-dependent for lidocaine. Eugenol and menthol were deemed safe and satisfactory for glucose analysis in zebrafish research, while lidocaine could introduce biases and safety concerns. Researchers should carefully consider the selection of anesthetics for reliable results in zebrafish assessments.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Xiaoxue Li, Tangsong Feng, Weiqun Lu
Summary: This study reveals that exposure to VPA induces ASD-like phenotypes and behaviors, including gene transcription changes, social interaction deficit, decreased aggression, increased anxiety behavior, and elevated cortisol levels. The aggression behavior assay is a better predictor of neurotoxicology behavior of VPA.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Garam An, Junho Park, Whasun Lim, Gwonhwa Song
Summary: In this study, we investigated the hepatotoxicity and mechanism of action of pyridaben using zebrafish and ZFL cells. Pyridaben caused liver size reduction and apoptosis, and these effects were associated with uncontrolled intracellular calcium flow and downregulation of the PI3K/Akt signaling cascade.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Md Alamgir Kabir, Md. Golam Rabbane, Marco R. Hernandez, Md Aftab Ali Shaikh, Mohammad Moniruzzaman, Xuexiu Chang
Summary: This study investigated the toxicity of high concentrations of cadmium (Cd) on intestinal immunity and microbial diversity in common carp. The results showed that high Cd concentration caused intestinal inflammation, immunosuppression, and changes in the gut microbiome, leading to detrimental effects on the intestinal homeostasis and health status of the fish.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Anuradha Batabyal, Veronica Rivi, Cristina Benatti, Johanna M. C. Blom, Fabio Tascedda, Ken Lukowiak
Summary: This study found that exposure to heat shock stressors and bacterial lipopolysaccharide can induce the Garcia effect in pond snails. Additionally, aspirin can prevent the formation of the Garcia effect. However, unexpectedly, exposure to crushed aspirin and heat shock stressors together leads to long-lasting feeding suppression and molecular changes in the snails' serotoninergic system and neuroplasticity genes.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Li-Yih Lin, Chieh-An Cheng, Sian-Tai Liu, Jiun-Lin Horng
Summary: This study reveals that ammonia exposure impairs ion regulation in zebrafish embryos by inducing oxidative stress, mitochondrial dysfunction, and cell death. The findings suggest that ammonia is toxic to aquatic animals and can lead to the death of embryos.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Antonio Durate Pagano, Natieli Machado Goncalves, William Borges Domingues, Tony Leandro Rezende da Silveira, Mateus Tavares Kutter, Antonio Sergio Varela Jr, Carine Dahl Corcini, Mariana Cavalcanti Nascimento, Luana Ferreira Viana dos Reis, Patricia Gomes Costa, Adalto Bianchini, Matheus Vieira Volcan, Mariana Harter Remia, Vinicius Farias Campos
Summary: This study analyzed the toxic effects of Roundup Transorb (R) on the endangered Neotropical annual killifish Austrolebias charrua by evaluating molecular and biochemical biomarkers. The findings showed that exposure to Roundup significantly increased the production of reactive oxygen species, lipid peroxidation, and DNA damage in the fish's blood cells. It also decreased membrane fluidity. Gene expression analysis revealed alterations in genes associated with oxidative stress and antioxidant defense. This study provides new insights into adaptive mechanisms of A. charrua in response to Roundup, and it has important implications for environmental monitoring and aquatic toxicology assessment.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Xiaochan Gao, Hongtao Ren, Yong Huang, Yimin Li, Jiaqi Shen
Summary: The influence of multi-walled carbon nanotubes (MWCNTs) on the toxicity of zinc oxide nanoparticles (ZnONPs) in the intestine of common carp was investigated. The study found that the concentration and concentration ratio of the mixture played a role in the toxicity of ZnONPs. The presence of MWCNTs significantly decreased intercellular zinc accumulation and altered the microbial community diversity and composition of the gut microbiota.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Guangchao Yang, Qian Yang, Trust Beta, Qin Liu, Zhenzhu Zhu, Fei Shen
Summary: The study found that black garlic MLDs have alleviative effects on ZEN-induced toxicity, and exert their effects through antioxidative, anti-apoptotic, and inhibitory mechanisms on ZEN absorption.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Tuul Sepp, Ciara Baines, Randel Kreitsberg, Joern Peter Scharsack, Pedro Nogueira, Thomas Lang, Jerome Fort, Elin Sild, John T. Clarke, Arvo Tuvikene, Richard Meitern
Summary: Environmental factors can cause cancer in both wild animals and humans. The use of polluted habitats provides opportunities to study cancer defense mechanisms, which can be influenced by genetic variation and natural selection. Flatfish species, specifically flounders, show higher resistance to pollution-induced cancer compared to dabs, with elevated pollutant metabolism and stronger tumor suppression mechanisms in their liver tissue. This suggests a potential hormetic upregulation of tumor suppression or stronger natural selection pressure for cancer resistance in flounders. Wild species offer valuable insights into understanding the nature and evolution of natural cancer defense mechanisms.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Regan A. Moss, Kaitlyn M. Murphy, Steven T. Gardner, Madison M. Watkins, John W. Finger Finger Jr, Meghan D. Kelley, Ruth M. Elsey, Daniel A. Warner, Mary T. Mendonca
Summary: The study explored the impact of environmentally relevant concentrations of E2 on the immune system and hormone levels of hatchling American alligators. Results showed that exposure to environmental estrogen can alter plasma hormone concentrations but have little to no impact on morphology and immune responses.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Ye Liang, Zihao Li, Jing Yuan, Yiwen Zhou, Meng Li, Haifeng Gu
Summary: This study investigated the toxicity mechanism of homo-YTX and NH3-N on the survival of abalone. The results showed that homo-YTX and NH3-N enhanced oxidative stress and lipid peroxidation reactions, inhibited energy supply, disrupted metabolic and immune physiological functions, and activated apoptosis in the gills of abalone.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)