Article
Multidisciplinary Sciences
Jay B. Lusk, Ellora Hui Zhen Chua, Prameet Kaur, Isabelle Chiao Han Sung, Wen Kin Lim, Vanessa Yuk Man Lam, Nathan Harmston, Nicholas S. Tolwinski
Summary: Proper embryonic development requires directional axes for patterning cells into embryonic structures. In Drosophila, the expression of transcription factors and the Toll and TGF beta signaling pathways play crucial roles in determining the anterior to posterior and dorsal to ventral patterning of the early embryo. In this study, we identified a novel loss of function mutation in the Raf kinase gene, which leads to the loss of ventral cell fates and reveals a novel role for Raf in embryonic patterning independent of the MAPK cascade.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Fangyu Song, Wenshuo Zhang, Xiaohui Li, Xiaoqing Chen, Xuejun Yuan, Mingjin Jiang, Yunhe Zhao, Qingxin Liu, Zizhang Zhou
Summary: The main cause of high mortality in cancer patients is tumor metastasis, and exploring the underlying mechanism of tumor metastasis is important for clinical treatments. This study identifies the transcription factor Apt/FSBP as a suppressor for tumor metastasis, providing it as a potential drug target for tumor treatment.
Article
Oncology
Oihane Erice, Shruthi Narayanan, Iker Feliu, Rodrigo Entrialgo-Cadierno, Antonia Malinova, Caterina Vicentini, Elizabeth Guruceaga, Pietro Del fi No, Marija Trajkovic-Arsic, Haritz Moreno, Karmele Valencia, Ester Blanco, Irati Macaya, Daniel Ohlund, Purvesh Khatri, Fernando Lecanda, Aldo Scarpa, Jens T. Siveke, Vincenzo Corbo, Mariano Ponz-Sarvise, Silve Vicent
Summary: In this study, the expression of LAMC2 in PDAC and its regulated network were investigated, with the aim of identifying potential therapies. It was found that LAMC2 was consistently upregulated in PDAC and associated with tumor grade and survival. The LAMC2-regulated network contained downstream effectors shared by the KRAS signaling pathway, and the inhibition of LAMC2 or AXL showed a synergistic antiproliferative effect in combination with MEK1/2 inhibitors in PDAC models.
CLINICAL CANCER RESEARCH
(2023)
Article
Biochemical Research Methods
Sandy Zhang, Tianhong Ouyang, Bjorn M. Reinhard
Summary: The multivalent presentation of EGF ligand on nanoparticles enhances EGF-induced ROS formation and EGFR phosphorylation in breast cancer cells.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Hematology
Imane Boukhatem, Samuel Fleury, Melanie Welman, Jessica Le Blanc, Chantal Thys, Kathleen Freson, Myron G. Best, Thomas Wurdinger, Bruce G. Allen, Marie Lordkipanidze
Summary: Research has shown that circulating BDNF levels appear to regulate platelet activation, aggregation, and secretion through activation of a truncated TrkB receptor and downstream kinase-dependent signaling. BDNF not only plays a role in neurons but also has important functions in platelets.
Article
Biochemistry & Molecular Biology
Tasuku Nishino, Takaya Abe, Mari Kaneko, Masanao Yokohira, Keiko Yamakawa, Katsumi Imaida, Hiroaki Konishi
Summary: This study found that the GAREM1 mutant has dominant-negative properties and cannot promote cell activation and proliferation, leading to idiopathic short stature in patients. In addition, mice deficient in GAREM1 also have a lighter weight. The study results suggest that GAREM1 is required for normal growth and maintaining average body size.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biology
Nil Vanli, Jinghao Sheng, Shuping Li, Zhengping Xu, Guo-Fu Hu
Summary: RNASE4 serves as a biomarker for prostate cancer and can predict aggressiveness of the disease and enhance diagnosis accuracy. It also promotes tumor cell proliferation and angiogenesis.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Fatih M. Uckun, Sanjive Qazi
Summary: The present study investigated the clinical significance of RNA sequencing-based ERBB2/HER2 expression level in malignant plasma cells from MM patients. Higher levels of ERBB2 mRNA were found in malignant plasma cells of MM patients, which had adverse impacts on patients' survival outcomes. This study suggests the potential of using ERBB2-targeting therapeutics to overcome cancer drug resistance in high-risk and relapsed/refractory MM.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Anna Gemza, Cinzia Barresi, Jakob Proemer, Jasmin Hatami, Margarita Lazaridis, Ruth Herbst
Summary: In the activation pathway of MuSK, only MuSK internalization is increased by Agrin stimulation, while Lrp4 remains unaffected, and MuSK kinase activity is insufficient to induce MuSK internalization. In addition, inhibition of Dynamin has no effect on MuSK internalization and signaling.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Review
Pharmacology & Pharmacy
Jazlyn P. Borges, Katrina Mekhail, Gregory D. Fairn, Costin N. Antonescu, Benjamin E. Steinberg
Summary: Chronic pain is a major public health issue that is often resistant to conventional analgesics. Recent studies have implicated the epidermal growth factor receptor (EGFR) signaling pathway in chronic pain, suggesting potential therapeutic targets for this devastating condition.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kyoko Nakajima, Shota Araki, Masahiro Kawahara
Summary: In this study, minimal synthetic receptors were engineered by incorporating multiple tyrosine motifs to reconstitute signaling properties. These receptors, activated by synthetic small-molecule ligands, were able to recruit and activate up to four signaling molecules of interest, offering potential for analyzing and controlling cell fate.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Xiaopeng Li, Jingjie Zhang, Hongyong Shi, Bo Li, Jia Li
Summary: Receptor-like kinases (RLKs) are a group of plant-specific transmembrane proteins that can convert extracellular signals into intracellular responses by altering gene expression profiles. Recent studies have found that many RLKs can physically interact with membrane-localized transport proteins, allowing for rapid responses in specific tissues or cells.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Review
Oncology
Mary Luz Uribe, Ilaria Marrocco, Yosef Yarden
Summary: Growth factors like epidermal growth factor (EGF) can promote cell division and migration, but cancer hijacks these mechanisms for tumor growth and metastasis. Over-production and mutations of EGF and EGFR are common in various cancers, making them popular targets for anti-cancer therapies. The activation of EGFR triggers a series of molecular switches controlling gene expression, with implications for drug resistance and metastasis.
Article
Cell Biology
Ching-Chia Cheng, Chih-Feng Lin, Yong-Chong Lin, Tai-Horng Young, Pei-Jen Lou
Summary: The study modified gene expression of ACs by transfection of the GnT-V-overexpression plasmid, leading to increased proliferation and stemness properties of ACs. High levels of GnT-V were positively correlated with ALDH1A3 expression by increasing phosphorylation of cell surface receptors and activating downstream signaling transduction. This indicates that GnT-V plays a significant role in cell immortalization through the EGFR/ERK/ALDH1A3 signaling pathway in the ACs model.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Oncology
Qing Chang, Huiping Qiang, Jialin Qian, Yuqiong Lei, Jiahuan Lu, Hui Feng, Yiming Zhao, Baohui Han, Yanwei Zhang, Tianqing Chu
Summary: For advanced Chinese female lung SCC patients with EGFR positive mutations, targeted therapy could confer longer progression-free survival (PFS) and overall survival (OS) than chemotherapy, but the survival was similar with patients who were negative EGFR mutations.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yehoshua Enuka, Mattia Lauriola, Morris E. Feldman, Aldema Sas-Chen, Igor Ulitsky, Yosef Yarden
NUCLEIC ACIDS RESEARCH
(2016)
Article
Medicine, Research & Experimental
Aldema Sas-Chen, Miriam R. Aure, Limor Leibovich, Silvia Carvalho, Yehoshua Enuka, Cindy Koerner, Maria Polycarpou-Schwarz, Sara Lavi, Nava Nevo, Yuri Kuznetsov, Justin Yuan, Francisco Azuaje, Igor Ulitsky, Sven Diederichs, Stefan Wiemann, Zohar Yakhini, Vessela N. Kristensen, Anne-Lise Borresen-Dale, Yosef Yarden
EMBO MOLECULAR MEDICINE
(2016)
Article
Multidisciplinary Sciences
Modi Safra, Aldema Sas-Chen, Roni Nir, Roni Winkler, Aharon Nachshon, Dan Bar-Yaacov, Matthias Erlacher, Walter Rossmanith, Noam Stern-Ginossar, Schraga Schwartz
Article
Biochemistry & Molecular Biology
Yehoshua Enuka, Morris E. Feldman, Animesh Chowdhury, Swati Srivastava, Moshit Lindzen, Aldema Sas-Chen, Renaud Massart, David Cheishvili, Matthew J. Suderman, Yoav Zaltsman, Chiara A. Mazza, Kirti Shukla, Cindy Koerner, Noa Furth, Mattia Lauriola, Moshe Oren, Stefan Wiemann, Moshe Szyf, Yosef Yarden
NUCLEIC ACIDS RESEARCH
(2017)
Article
Biochemistry & Molecular Biology
Lee Roth, Swati Srivastava, Moshit Lindzen, Aldema Sas-Chen, Michal Sheffer, Mattia Lauriola, Yehoshua Enuka, Ashish Noronha, Maicol Mancini, Sara Lavi, Gabi Tarcic, Gur Pines, Nava Nevo, Ori Heyman, Tamar Ziv, Oscar M. Rueda, Davide Gnocchi, Eli Pikarski, Arie Admon, Carlos Caldas, Yosef Yarden
Article
Biochemistry & Molecular Biology
Lee Roth, Swati Srivastava, Moshit Lindzen, Aldema Sas-Chen, Michal Sheffer, Mattia Lauriola, Yehoshua Enuka, Ashish Noronha, Maicol Mancini, Sara Lavi, Gabi Tarcic, Gur Pines, Nava Nevo, Ori Heyman, Tamar Ziv, Oscar M. Rueda, Davide Gnocchi, Eli Pikarski, Arie Admon, Carlos Caldas, Yosef Yarden
Review
Biochemistry & Molecular Biology
Giovanni Blandino, Francesco Fazi, Sara Donzelli, Merav Kedmi, Aldema Sas-Chen, Paola Muti, Sabrina Strano, Yosef Yarden
Article
Chemistry, Multidisciplinary
Justin M. Thomas, Chloe A. Briney, Kellie D. Nance, Jeffrey E. Lopez, Abigail L. Thorpe, Stephen D. Fox, Marie-Line Bortolin-Cavaille, Aldema Sas-Chen, Daniel Arango, Shalini Oberdoerffer, Jerome Cavaille, Thorkell Andresson, Jordan L. Meier
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2018)
Article
Biochemical Research Methods
Aldema Sas-Chen, Schraga Schwartz
Article
Multidisciplinary Sciences
Aldema Sas-Chen, Justin M. Thomas, Donna Matzov, Masato Taoka, Kellie D. Nance, Ronit Nir, Keri M. Bryson, Ran Shachar, Geraldy L. S. Liman, Brett W. Burkhart, Supuni Thalalla Gamage, Yuko Nobe, Chloe A. Briney, Michaella J. Levy, Ryan T. Fuchs, G. Brett Robb, Jesse Hartmann, Sunny Sharma, Qishan Lin, Laurence Florens, Michael P. Washburn, Toshiaki Isobe, Thomas J. Santangelo, Moran Shalev-Benami, Jordan L. Meier, Schraga Schwartz
Article
Biotechnology & Applied Microbiology
Oguzhan Begik, Morghan C. Lucas, Leszek P. Pryszcz, Jose Miguel Ramirez, Rebeca Medina, Ivan Milenkovic, Sonia Cruciani, Huanle Liu, Helaine Graziele Santos Vieira, Aldema Sas-Chen, John S. Mattick, Schraga Schwartz, Eva Maria Novoa
Summary: This study demonstrated the potential of nanopore RNA sequencing for detecting and identifying various RNA modifications, including pseudouridine and 2'-O-methylation. By focusing on pseudouridine modification sites, the researchers revealed the distribution and dynamics of these modifications in cellular RNAs. The development of a software tool to estimate modification stoichiometries at each site provides a valuable tool for further research on RNA modifications.
NATURE BIOTECHNOLOGY
(2021)
Article
Biochemical Research Methods
Supuni Thalalla Gamage, Aldema Sas-Chen, Schraga Schwartz, Jordan L. Meier
Summary: The N4-acetylcytidine RNA modification is conserved in all domains of life. The authors present a detailed protocol for quantitative nucleotide resolution mapping of this modification, introducing N4-acetylcytidine sequencing (ac4C-seq) as a new method for accurate identification of specific ac4C residues and their level of modification. This protocol can be completed in approximately 4 days for transcriptome-wide sequencing.
Article
Biochemistry & Molecular Biology
Ronit Nir, Thomas Philipp Hoernes, Hiromi Muramatsu, Klaus Faserl, Katalin Kariko, Matthias David Erlacher, Aldema Sas-Chen, Schraga Schwartz
Summary: RNA can be modified post-transcriptionally with various modifications, including pseudouridine (psi), which has been found in mRNA molecules. This study investigates the possibility of snoRNA-guided pseudouridylation of mRNA targets and provides insights into the factors affecting the efficiency of this modification. The study also explores the potential therapeutic application of pseudouridylation in suppressing translational termination.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Aldema Sas-Chen, Swati Srivastava, Yosef Yarden
BIOCHEMICAL SOCIETY TRANSACTIONS
(2017)