Article
Biophysics
Wuzhen Qi, Lingyan Zheng, Sihan Wang, Fengchun Huang, Yuanjie Liu, Haiyang Jiang, Jianhan Lin
Summary: A microfluidic biosensor was developed for rapid, sensitive, and automatic detection of Salmonella in contaminated foods. The biosensor showed the ability to detect Salmonella Typhimurium with a detection limit of 14 CFU/mL in just 1 hour. The integration of mixing, separation, labelling, and detection on a single microfluidic chip demonstrated automatic operation, fast reaction, less reagent usage, and small size, making it promising for in-field detection of foodborne bacteria.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Engineering, Environmental
Soo Chung, Andrew Loh, Christian M. Jennings, Katelyn Sosnowski, Sung Yong Ha, Un Hyuk Yim, Jeong-Yeol Yoon
Summary: We developed a novel method to screen different types of oil using a wax-printed paper-based microfluidic platform. The oil samples flowed through micrometer-scale channels via capillary action, while their components were filtered and separated. The fluctuation in the capillary flow velocity profiles was utilized to classify oil types. A Raspberry Pi camera captured video clips, which were analyzed using a custom Python code to obtain the capillary flow velocity profiles. 106 velocity profiles were recorded from various oil samples, and principal component analysis, support vector machine, and linear discriminant analysis were used to classify the oil types with high accuracy.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Multidisciplinary Sciences
Abbas Jalili, Maryam Bagheri, Amir Shamloo, Amir Hossein Kazemipour Ashkezari
Summary: A rapid droplet-based photonic PCR system was developed using a gold nanofilm-based microfluidic chip and a plasmonic photothermal cycler, achieving fast thermal cycling and successful amplification of the AOX gene. The microfluidic chip demonstrated great performance in droplet-based PCR.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Gangwei Xu, Huaqing Si, Fengxiang Jing, Peng Sun, Dongping Wu
Summary: A self-priming microfluidic chip based on PDMS with cushion chambers was proposed for robust and easy dPCR. The chip uses negative pressure and cushion chambers to autonomously partition samples, showing excellent performance in absolute quantification of target genes.
Article
Biochemical Research Methods
Yuling Qin, Jason E. Kreutz, Thomas Schneider, Gloria S. Yen, Eleanor S. Shah, Li Wu, Daniel T. Chiu
Summary: This paper develops a reinforced PDMS mold for hot embossing of COP sheets and demonstrates its application in the fabrication of microfluidic chips. The method allows for rapid and cost-effective preparation of high precision and highly reproducible microscale features on the chips.
Article
Chemistry, Analytical
Zhiwei Qin, Xinran Xiang, Liang Xue, Weicheng Cai, Junshan Gao, Jiale Yang, Yanhui Liang, Linping Wang, Moutong Chen, Rui Pang, Ying Li, Jumei Zhang, Yongdan Hu, Qingping Wu
Summary: A rapid and cost-effective digital nucleic acid detection method based on a microfluidic chip was developed for norovirus detection. The method showed high sensitivity and robustness, demonstrating its potential as an alternative approach for early norovirus detection and epidemic prevention and control.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Youngseop Lee, Eun-Sil Yu, Hamin Na, Minhee Kang, Hee Jae Huh, Ki-Hun Jeong
Summary: The rapid and accurate molecular diagnostics are crucial in the face of pandemic diseases, and the nanoplasmonic PCR provides a new solution for rapid and quantitative molecular diagnostics at point-of-care level.
Article
Chemistry, Analytical
Phenix-Lan Quan, Maria Alvarez-Amador, Yuhe Jiang, Martin Sauzade, Eric Brouzes
Summary: This article reports the design of a thermoplastic partitioning device that enables efficient and reliable partitioning, operated by a simple micropipette at low pressure. The device provides a cost-effective and instrument-free partitioning platform for low-resource settings.
Article
Food Science & Technology
Xinran Xiang, Enjie Diao, Yuting Shang, Minghui Song, Yinglong He
Summary: With the rapid development of logistics, there is an urgent need to develop effective detection strategies to ensure food safety, as a growing number of pathogenic microorganisms spread worldwide using food as a carrier. This study developed a high-fidelity target-based microfluidic identification (HFTMI) strategy by combining novel markers identified by pan-genome analysis and a digital recombinase-aided amplification (RAA) detection method. The HFTMI system showed improved performance compared to qPCR, providing more reliable guidance for food safety testing.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biochemical Research Methods
Charlotte Parent, Patricia Laurent, Charles-Elie Goujon, Xavier Mermet, Armelle Keiser, Francois Boizot, Raymond Charles, Lucas Audebert, Yves Fouillet, Myriam Cubizolles
Summary: A microfluidic platform for the integration of multi-step biological assays has been developed. The platform is versatile and can be used for various biological assays, saving development time. It also features a fully automated protocol with good sensitivity and specificity.
Article
Biochemical Research Methods
Tengbao Xie, Ping Wang, Lei Wu, Bangyong Sun, Qiang Zhao, Gang Li
Summary: The study introduces a simple system for sample discretization, using water-soluble film and a syringe to rapidly partition samples, which can be applied in digital PCR and other fields such as single-cell analysis and diagnostics.
Article
Chemistry, Multidisciplinary
Chung-Hyun Lee, Woo-Hyuk Lee, Sung-Min Kim
Summary: An IoT-based fire detection system was developed to detect and prevent damage from forest fires at an early stage. The system uses a Raspberry Pi and a fisheye camera for real-time fire detection. Images are analyzed using the OpenCV library, and the location of the fire is estimated using polar coordinates. The system was tested in a mountainous area and showed promising results with a small positional error.
APPLIED SCIENCES-BASEL
(2023)
Article
Engineering, Chemical
Dominik M. Loy, Rafal Krzyszton, Ulrich Laechelt, Joachim O. Raedler, Ernst Wagner
Summary: Formulating nanoparticles for APIs with suboptimal pharmacokinetic properties requires precise control, with microfluidic platforms offering potential advantages. A low-budget modular prototype for microfluidic mixing is described, comprising control, feeding, formulation, and collection modules. The prototype demonstrates efficacy in formulating polyplexes from siRNA and oligomers at different stages of nanoparticle assembly.
Article
Biotechnology & Applied Microbiology
Kangning Wang, Bin Li, Yu Guo, Yanqi Wu, Yan Li, Wenming Wu
Summary: We have developed a low-cost digital PCR system with higher reagent universality compared to commercial systems. Our system can detect DNA that cannot be detected by commercial systems due to special reagent formulations, and calculate their concentration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Immunology
Di Wang, Xinya Jiao, Haijiang Jia, Shumei Cheng, Xi Jin, Youhua Wang, Yunhua Gao, Xiaofeng Su
Summary: This study developed a droplet digital PCR (ddPCR) assay for accurate and sensitive detection and quantification of Verticillium dahliae and V. longisporum, showing that ddPCR is more sensitive and accurate than qPCR for quantifying trace amounts of these pathogens in field samples.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Analytical
Xiong Ding, Kevin D. Clark, Marcelino Varona, Miranda N. Emaus, Jared L. Anderson
ANALYTICA CHIMICA ACTA
(2019)
Review
Chemistry, Analytical
Xiong Ding, Michael G. Mauk, Kun Yin, Karteek Kadimisetty, Changchun Liu
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Analytical
Xiong Ding, Guoping Wang, Ying Mu
ANALYTICA CHIMICA ACTA
(2019)
Article
Chemistry, Analytical
Kun Yin, Xiong Ding, Ziyue Li, Hui Zhao, Kumarasen Cooper, Changchun Liu
ANALYTICAL CHEMISTRY
(2020)
Article
Multidisciplinary Sciences
Xiong Ding, Kun Yin, Ziyue Li, Rajesh V. Lalla, Enrique Ballesteros, Maroun M. Sfeir, Changchun Liu
NATURE COMMUNICATIONS
(2020)
Article
Chemistry, Analytical
Wenshuai Wu, Fengtian Wu, Shan Zhang, Xiong Ding, Tao Zhang, Ying Yang, Ying Mu
Article
Biophysics
Zhiheng Xu, Kun Yin, Xiong Ding, Ziyue Li, Xuanhao Sun, Baikun Li, Rajesh V. Lalla, Robert Gross, Changchun Liu
Summary: A simple, disposable, and integrated electronic-tube cap (E-tube cap) for DNA detection at the point-of-care was developed and successfully demonstrated the presence of 100 copies of λDNA in saliva samples. The device showed fast, accurate, and cost-effective diagnostic capabilities, making it particularly suitable for point-of-care molecular diagnostics of infectious diseases.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biophysics
Xiong Ding, Kun Yin, Ziyue Li, Maroun M. Sfeir, Changchun Liu
Summary: The study introduces a digital warm-start CRISPR assay for sensitive quantitative detection of SARS-CoV-2 in clinical samples, overcoming undesired premature target amplification at room temperature. The method, targeting SARS-CoV-2's nucleoprotein gene, can detect down to 5 copies/ul SARS-CoV-2 RNA in the chip and has been clinically validated. Additionally, it can directly detect SARS-CoV-2 in heat-treated saliva samples without RNA extraction, making it a sensitive and reliable CRISPR assay for accurate SARS-CoV-2 detection.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biophysics
Ziyue Li, Xiong Ding, Kun Yin, Zhiheng Xu, Kumarasen Cooper, Changchun Liu
Summary: The developed EFE electrochemical CRISPR biosensor is a simple, versatile tool for detecting DNA targets in a homogeneous solution phase, achieving sensitive detection without the need for complicated immobilization processing. By leveraging electric field enhancement and diffusivity differences, the sensor provides a robust and sensitive method for nucleic acid-based molecular diagnostics.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Kun Yin, Xiong Ding, Zhiheng Xu, Ziyue Li, Xingyu Wang, Hui Zhao, Clifford Otis, Baikun Li, Changchun Liu
Summary: The study developed a sensitive, multiplexed, colorimetric detection method for pathogen detection in wastewater samples, allowing for early warning of disease outbreaks. This method integrated on-chip nucleic acid extraction, two-stage isothermal amplification, and colorimetric detection on a 3D printed microfluidic chip, enabling real-time analysis without complex equipment.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Yaru Wang, Lanxiang Zhang, Yuqing Shen, Evan Yi-Wen Yu, Xiong Ding
Summary: This article introduces a nested phosphorothioated (PS) hybrid primer-mediated isothermal amplification (NPSA) assay, which achieves specific and dye-based subattomolar nucleic acid detection at 37 degrees C using EvaGreen as a DNA-binding dye. The high efficiency of this assay relies on nested PS-modified hybrid primers, as well as the additives of urea and T4 Gene 32 Protein. To overcome the inhibitory effect of urea on reverse transcription, a one-tube two-stage recombinase-aided RT-NPSA (rRT-NPSA) method is established. The NPSA/rRT-NPSA assays show consistent results with PCR/RT-PCR methods for qualitatively detecting DNA/mRNA targets.
Article
Biochemical Research Methods
Kun Yin, Xiong Ding, Ziyue Li, Maroun M. Sfeir, Enrique Ballesteros, Changchun Liu
Summary: The study presents an autonomous lab-on-paper platform for multiplex gene diagnosis of SARS-CoV-2, capable of simultaneously detecting nucleoprotein (N) gene, spike (S) gene, and human housekeeping RNAse P gene. This platform shows great potential for rapid, sensitive, reliable, and multiple molecular diagnostics of COVID-19 and other infectious diseases in resource-limited settings.
Article
Multidisciplinary Sciences
Xiong Ding, Kun Yin, Ziyue Li, Vikram Pandian, Joan A. Smyth, Zeinab Helal, Changchun Liu
SCIENTIFIC REPORTS
(2020)
Article
Chemistry, Multidisciplinary
Xiong Ding, Kun Yin, Ju Chen, Kepeng Wang, Changchun Liu
CHEMICAL COMMUNICATIONS
(2019)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)