Directed evolution of cytochrome P450 enzymes for biocatalysis: exploiting the catalytic versatility of enzymes with relaxed substrate specificity

Improved Cyclopropanation Activity of Histidine-Ligated Cytochrome P450 Enables the Enantioselective Formal Synthesis of Levomilnacipran

(2014) Z. Jane Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Enantioselective Imidation of Sulfides via Enzyme-Catalyzed Intermolecular Nitrogen-Atom Transfer

(2014) Christopher C. Farwell et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Enantioselective Intramolecular CH Amination Catalyzed by Engineered Cytochrome P450 Enzymes In Vitro and In Vivo

(2013) John A. McIntosh et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Light-driven biocatalysis with cytochrome P450 peroxygenases

(2013) Marco Girhard et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

On the development of new biocatalytic processes for practical pharmaceutical synthesis

(2013) Gjalt W Huisman et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

A serine-substituted P450 catalyzes highly efficient carbene transfer to olefins in vivo

(2013) Pedro S Coelho et al.   Nature Chemical Biology

Origins of P450 diversity

(2013) H. Sezutsu et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Cytochrome P450-catalyzed insertion of carbenoids into N–H bonds

(2013) Z. Jane Wang et al.   Chemical Science

Biotechnological Production of Caffeic Acid by Bacterial Cytochrome P450 CYP199A2

(2012) Toshiki Furuya et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Interaction of human cytochrome P4503A4 with ritonavir analogs

(2012) Irina F. Sevrioukova et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

The monooxygenase, peroxidase, and peroxygenase properties of cytochrome P450

(2012) Eugene G. Hrycay et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Conformational Adaptation of Human Cytochrome P450 2B6 and Rabbit Cytochrome P450 2B4 Revealed upon Binding Multiple Amlodipine Molecules

(2012) Manish B. Shah et al.   BIOCHEMISTRY

Production of human cytochrome P450 2D6 drug metabolites with recombinant microbes - a comparative study

(2012) Martina Geier et al.   Biotechnology Journal

Pyran Formation by an Atypical CYP-Mediated Four-Electron Oxygenation-Cyclization Cascade in an Engineered Aureothin Pathway

(2012) Martin Richter et al.   CHEMBIOCHEM

Many Pathways in Laboratory Evolution Can Lead to Improved Enzymes: How to Escape from Local Minima

(2012) Yosephine Gumulya et al.   CHEMBIOCHEM

Directed Evolution Reveals Requisite Sequence Elements in the Functional Expression of P450 2F1 in Escherichia coli

(2012) James B. Y. H. Behrendorff et al.   CHEMICAL RESEARCH IN TOXICOLOGY

Predicting Drug Metabolism by Cytochrome P450 2C9: Comparison with the 2D6 and 3A4 Isoforms

(2012) Patrik Rydberg et al.   ChemMedChem

Crystal Structure of Human Cytochrome P450 2D6 with Prinomastat Bound

(2012) An Wang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

2D SMARTCyp Reactivity-Based Site of Metabolism Prediction for Major Drug-Metabolizing Cytochrome P450 Enzymes

(2012) Ruifeng Liu et al.   Journal of Chemical Information and Modeling

Controlled Oxidation of Remote sp3 C–H Bonds in Artemisinin via P450 Catalysts with Fine-Tuned Regio- and Stereoselectivity

(2012) Kaidong Zhang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Caffeic Acid, A Versatile Pharmacophore: An Overview

(2012) M. Touaibia et al.   MINI-REVIEWS IN MEDICINAL CHEMISTRY

Olefin Cyclopropanation via Carbene Transfer Catalyzed by Engineered Cytochrome P450 Enzymes

(2012) P. S. Coelho et al.   SCIENCE

Network Context and Selection in the Evolution to Enzyme Specificity

(2012) H. Nam et al.   SCIENCE

Terminal Olefin (1-Alkene) Biosynthesis by a Novel P450 Fatty Acid Decarboxylase fromJeotgalicoccusSpecies

(2011) Mathew A. Rude et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Structural Analysis of Mammalian Cytochrome P450 2B4 Covalently Bound to the Mechanism-Based Inactivatortert-Butylphenylacetylene: Insight into Partial Enzymatic Activity

(2011) Sean C. Gay et al.   BIOCHEMISTRY

The Moderately Efficient Enzyme: Evolutionary and Physicochemical Trends Shaping Enzyme Parameters

(2011) Arren Bar-Even et al.   BIOCHEMISTRY

P450BM3(CYP102A1): connecting the dots

(2011) Christopher J. C. Whitehouse et al.   CHEMICAL SOCIETY REVIEWS

An Expanded, Unified Substrate Recognition Site Map for Mammalian Cytochrome P450s: Analysis of Molecular Interactions Between 15 Mammalian CYP450 Isoforms and 868 Substrates

(2011) Alexander Zawaira et al.   CURRENT DRUG METABOLISM

Cytochrome P450: taming a wild type enzyme

(2011) Sang Taek Jung et al.   CURRENT OPINION IN BIOTECHNOLOGY

Efficient Screening of Cytochrome P450 BM3 Mutants for Their Metabolic Activity and Diversity toward a Wide Set of Drug-Like Molecules in Chemical Space

(2011) J. Reinen et al.   DRUG METABOLISM AND DISPOSITION

Structural and Mechanistic Insights into the Interaction of Cytochrome P4503A4 with Bromoergocryptine, a Type I Ligand

(2011) Irina F. Sevrioukova et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

IDSite: An Accurate Approach to Predict P450-Mediated Drug Metabolism

(2011) Jianing Li et al.   Journal of Chemical Theory and Computation

Optimization of Combinatorial Mutagenesis

(2011) Andrew S. Parker et al.   JOURNAL OF COMPUTATIONAL BIOLOGY

P450 Fingerprinting Method for Rapid Discovery of Terpene Hydroxylating P450 Catalysts with Diversified Regioselectivity

(2011) Kaidong Zhang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Structural Fine-Tuning of a Multifunctional Cytochrome P450 Monooxygenase

(2011) Georg Zocher et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Facile production of minor metabolites for drug development using a CYP3A shuffled library

(2011) D.J.B. Hunter et al.   METABOLIC ENGINEERING

Ligand-Based Site of Metabolism Prediction for Cytochrome P450 2D6

(2011) Patrik Rydberg et al.   ACS Medicinal Chemistry Letters

Characterization of the versatile monooxygenase CYP109B1 from Bacillus subtilis

(2010) Marco Girhard et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Simultaneous measurement of CYP1A2 activity, regioselectivity, and coupling: Implications for environmental sensitivity of enzyme–substrate binding

(2010) Matthew J. Traylor et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Recombinant human cytochrome P450 monooxygenases for drug metabolite synthesis

(2010) Kirsten Schroer et al.   BIOTECHNOLOGY AND BIOENGINEERING

Inverting the enantioselectivity of P450pyr monooxygenase by directed evolution

(2010) Weng Lin Tang et al.   CHEMICAL COMMUNICATIONS

Screening of a minimal enriched P450 BM3 mutant library for hydroxylation of cyclic and acyclic alkanes

(2010) Evelyne Weber et al.   CHEMICAL COMMUNICATIONS

Status of protein engineering for biocatalysts: how to design an industrially useful biocatalyst

(2010) Andreas S Bommarius et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Engineering Bacterial Cytochrome P450 (P450) BM3 into a Prototype with Human P450 Enzyme Activity Using Indigo Formation

(2010) S. H. Park et al.   DRUG METABOLISM AND DISPOSITION

Plasticity of Cytochrome P450 2B4 as Investigated by Hydrogen-Deuterium Exchange Mass Spectrometry and X-ray Crystallography

(2010) P. Ross Wilderman et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural Evidence for Enhancement of Sequential Vitamin D3Hydroxylation Activities by Directed Evolution of Cytochrome P450 Vitamin D3Hydroxylase

(2010) Yoshiaki Yasutake et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Cytochrome P450 Compound I: Capture, Characterization, and C-H Bond Activation Kinetics

(2010) J. Rittle et al.   SCIENCE

Computational Design of an Enzyme Catalyst for a Stereoselective Bimolecular Diels-Alder Reaction

(2010) J. B. Siegel et al.   SCIENCE

Application of cytochrome P450 BM3 mutants as biocatalysts for the profiling of estrogen receptor binding metabolites of the mycotoxin zearalenone

(2010) Jelle Reinen et al.   XENOBIOTICA

A Diversified Library of Bacterial and Fungal Bifunctional Cytochrome P450 Enzymes for Drug Metabolite Synthesis

(2009) Roland Weis et al.   ADVANCED SYNTHESIS & CATALYSIS

Purification, characterization, and directed evolution study of a vitamin D 3 hydroxylase from Pseudonocardia autotrophica

(2009) Yoshikazu Fujii et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Crystal Structures of Cytochrome P450 2B4 in Complex with the Inhibitor 1-Biphenyl-4-methyl-1H-imidazole: Ligand-Induced Structural Response through α-Helical Repositioning,

(2009) Sean C. Gay et al.   BIOCHEMISTRY

Directed Evolution of the Actinomycete Cytochrome P450 MoxA (CYP105) for Enhanced Activity

(2009) Hiroki KABUMOTO et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Rational Design of a Minimal and Highly Enriched CYP102A1 Mutant Library with Improved Regio-, Stereo- and Chemoselectivity

(2009) Alexander Seifert et al.   CHEMBIOCHEM

Structure-based Drug Metabolism Predictions for Drug Design

(2009) Hao Sun et al.   Chemical Biology & Drug Design

A Panel of Cytochrome P450 BM3 Variants to Produce Drug Metabolites and Diversify Lead Compounds

(2009) Andrew M. Sawayama et al.   CHEMISTRY-A EUROPEAN JOURNAL

Algorithms for Joint Optimization of Stability and Diversity in Planning Combinatorial Libraries of Chimeric Proteins

(2009) Wei Zheng et al.   JOURNAL OF COMPUTATIONAL BIOLOGY

Mechanistic aspects of CYP74 allene oxide synthases and related cytochrome P450 enzymes

(2009) Alan R. Brash   PHYTOCHEMISTRY

Chemoenzymatic elaboration of monosaccharides using engineered cytochrome P450BM3 demethylases

(2009) J. C. Lewis et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single Mutations Change CYP2F3 From a Dehydrogenase of 3-Methylindole to an Oxygenase†

(2008) Jaya S. Kartha et al.   BIOCHEMISTRY

The activity of human CYP2D6 in low water organic solvents

(2008) Jin Zhao et al.   BIOTECHNOLOGY AND BIOENGINEERING

Evolved CYP102A1 (P450BM3) variants oxidise a range of non-natural substrates and offer new selectivity options

(2008) Christopher J. C. Whitehouse et al.   CHEMICAL COMMUNICATIONS

Molecular Characterization of CYP2B6 Substrates

(2008) Sean Ekins et al.   CURRENT DRUG METABOLISM

Identification of amino acid residues involved in 4-chloroindole 3-hydroxylation by cytochrome P450 2A6 using screening of random libraries

(2008) Zhi-Gang Zhang et al.   JOURNAL OF BIOTECHNOLOGY

Improvement of Cyclophosphamide Activation by CYP2B6 Mutants: From in Silico to ex Vivo

(2008) T.-A. Nguyen et al.   MOLECULAR PHARMACOLOGY

Kemp elimination catalysts by computational enzyme design

(2008) Daniela Röthlisberger et al.   NATURE

Chemo-enzymatic fluorination of unactivated organic compounds

(2008) Andrea Rentmeister et al.   Nature Chemical Biology

Development of a New Predictive Model for Interactions with Human Cytochrome P450 2A6 Using Pharmacophore Ensemble/Support Vector Machine (PhE/SVM) Approach

(2008) Max K. Leong et al.   PHARMACEUTICAL RESEARCH

De Novo Computational Design of Retro-Aldol Enzymes

(2008) L. Jiang et al.   SCIENCE