4.6 Article

Production and characterization of PHB from a novel isolate Comamonas sp from a dairy effluent sample and its application in cell culture

Journal

BIOCHEMICAL ENGINEERING JOURNAL
Volume 101, Issue -, Pages 150-159

Publisher

ELSEVIER
DOI: 10.1016/j.bej.2015.05.012

Keywords

Poly-3-hydroxybutyrate; Comamonas sp.; Fermentation; Cell culture; Matrices; Optimization

Funding

  1. DBT

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In the present study, media engineering was carried out to optimize various process parameters affecting fermentation for PHB production by Comamonas sp. The optimum conditions for PHB production were yeast extract concentration of 1%, pH 6.0 and inoculum concentration of 5.5%. Under optimized conditions the PHB yield was 0.523 g/g. The extracted polymer was characterized by FTIR and H-1 NMR. Nanomatrices and solid walled matrices were prepared using the extracted PHB. Cytotoxic studies revealed that both solid walled and nanomatrices prepared from the extracted PHB showed almost 100% survivals of H9c2 cells. This is the first report on application of extracted PHB from Comamonas sp. as an extracellular matrix for cell culture. (C) 2015 Elsevier B.V. All rights reserved.

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