Journal
BIOMASS CONVERSION AND BIOREFINERY
Volume 5, Issue 3, Pages 247-255Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s13399-014-0138-2
Keywords
Spent tea waste; Ethanol; Fermentation; Plackett-Burman design; Central composite design
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Funding
- Mustafa Kemal University Research foundation [245]
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The aim of this study was to investigate the prospect for the use of spent tea waste (STW), an important municipal waste, as a potential substrate to generate hydroly-sates for fuel ethanol production. Acid pretreated STW was used as substrate for ethanol production. The critical variables that affected ethanol fermentation from STW were identified by Plackett-Burman designs and further optimized by using a five-level-three-factor central composite design of response surface methodology. The optimum conditions for ethanol fermentation were determined to be NH4Cl concentration of 2.7 g/L, yeast concentration of 11.7 g/L, and temperature of 42.8 degrees C. Maximum concentration of reducing sugar and ethanol under the optimum conditions were 28.90 g reducing sugar/L and 12.72 g EtOH/L, respectively. Predicted ethanol concentration was obtained using quadratic polynomial equation. The predicted ethanol concentration was 13.38 g EtOH/L in the optimal conditions. Validity of the predicted model was confirmed using verification experiment (12.72 g EtOH/L).
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