Article
Biochemistry & Molecular Biology
Dipali Goyal, Sean W. Limesand, Ravi Goyal
Summary: A stem cell-like population is found in fetal carotid arteries, and the survival of these cells depends on the B-Raf kinase.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Marc Vila Cuenca, Amy Cochrane, Francijna E. van den Hil, Antoine A. F. de Vries, Saskia A. J. Lesnik Oberstein, Christine L. Mummery, Valeria V. Orlova
Summary: The study developed a 3D vessel-on-chip model based on human induced pluripotent stem cells to recapitulate the crosstalk between endothelial cells and pericytes or vascular smooth muscle cells, enabling the study of vascular (patho)physiology and therapy. The hiPSC-derived vascular cells organized into stable microvascular networks within a fibrin hydrogel microenvironment and responded to vasoactive stimulation, demonstrating the model's potential for quantifying vascular organization and intracellular Ca2+ release kinetics.
Review
Cell Biology
France Dierick, Julien Solinc, Juliette Bignard, Florent Soubrier, Sophie Nadaud
Summary: This review summarizes the importance of cellular processes and dysfunction in the development of pulmonary arterial hypertension, as well as the roles of circulating and resident vascular cells in pulmonary vascular remodeling.
Article
Biochemical Research Methods
Elizabeth E. Crouch, Loukas N. Diafos, Edward J. Valenzuela, Kaylee Wedderburn-Pugh, Janeth Ochoa Birrueta, Jaela Caston, Tara Joseph, Madeline G. Andrews, Aparna Bhaduri, Eric J. Huang
Summary: Angiogenesis and neurogenesis are functionally interconnected during brain development. This article presents a method to purify prenatal human brain vascular cells using FACS and utilize them in various downstream applications. The method is simple and efficient, generating high yields from small amounts of tissue. These vascular cells can be cultured and used in various in vitro assays. Additionally, the article describes how to use these cells for transplantation into cortical organoids to study neurovascular interactions in prenatal human brain development.
Article
Cell Biology
Michael R. Williamson, Cathleen Joy A. Fuertes, Andrew K. Dunn, Michael R. Drew, Theresa A. Jones
Summary: This study demonstrates that reactive astrocytes play a crucial role in vascular repair and remodeling after ischemic stroke in mice, by activating transcriptional programs related to vascular remodeling. Ablation of reactive astrocytes significantly impairs vascular and extracellular matrix remodeling, leading to prolonged blood flow deficits, exacerbated vascular permeability, ongoing cell death, and worsened motor recovery. These findings highlight the critical role of reactive astrocytes as cellular mediators of vascular remodeling during neural repair.
Article
Multidisciplinary Sciences
Isabella Bittencourt do Valle, Sicilia Rezende Oliveira, Janine Mayra da Silva, Gabriela Tonini Peterle, Anna Clara Gregorio Co, Sebastiao Silverio Sousa-Neto, Elismauro Francisco Mendonca, Jose Alcides Almeida de Arruda, Natalia Aparecida Gomes, Gabriel da Silva, Andreia Machado Leopoldino, Soraia Macari, Alexander Birbrair, Sandra Ventorin von Zeidler, Ivana Marcia Alves Diniz, Tarcilia Aparecida Silva
Summary: This study investigates the role of pericytes in oral squamous cell carcinoma (OSCC) and finds that they interact with neoplastic cells, influencing cancer progression and outcomes. The study characterizes pericytes in clinical samples and a mouse model of oral carcinogenesis, and shows that an increase in pericyte numbers is associated with the development of oral cancer.
SCIENTIFIC REPORTS
(2023)
Article
Cell Biology
Seley Gharanei, Katherine Fishwick, Ruban Peter Durairaj, Tianrong Jin, Eleftherios Siamantouras, Kuo-Kang Liu, Anne Straube, Emma S. Lucas, Christopher J. Weston, Pia Rantakari, Marko Salmi, Sirpa Jalkanen, Jan J. Brosens, Bee Kang Tan
Summary: The study reveals that pericytes in the midluteal human endometrium express VAP-1, and its knockdown affects their biophysical properties and interactions with uterine natural killer cells. This suggests a potential role for VAP-1 as a biomarker for pregnancy pathologies related to compromised perivascular environment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Takanori Sano, Tadaaki Nakajima, Koharu Alicia Senda, Shizuka Nakano, Mizuho Yamato, Yukinori Ikeda, Hedele Zeng, Jun-ichi Kawabe, Yukiko T. T. Matsunaga
Summary: This study used a 3D in vitro model to analyze the interaction between endothelial cells (ECs) and mesenchymal stem cells (MSCs). Through immunofluorescence imaging, it was found that MSCs can smooth the surface of microvessels and elongate angiogenic sprouts. Additionally, three-dimensional modeling revealed that MSCs selectively localize around protrusions or roots of angiogenic sprouts. This study demonstrates the important role of MSCs in the activation and stabilization of capillary vessels.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Chemistry, Multidisciplinary
Wenjin Cai, Jinglun Zhang, Yiqian Yu, Yueqi Ni, Yan Wei, Yihong Cheng, Litian Han, Leyi Xiao, Xiaoxin Ma, Hongjiang Wei, Yaoting Ji, Yufeng Zhang
Summary: Mitochondrial transfer from macrophages to mesenchymal stem cells regulates bone homeostasis, and abnormal transfer under osteoporotic conditions leads to metabolic remodeling in stem cells.
Article
Hematology
Ruth-Miriam Koerber, Rebekka K. Schneider, Jessica Ellen Pritchard, Lino L. Teichmann, Udo Schumacher, Peter Brossart, Ines Guetgemann
Summary: In this study, we investigated the cellular expression of nestin in bone marrow trephine biopsies of MPN patients. The results showed that nestin expression following myeloablation is inversely correlated with cellularity in the bone marrow. Nestin is highly expressed in mature osteocytes, arteriolar endothelial and perivascular cells, and small capillaries within the bone marrow space.
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Article
Biology
Xiao Su, Xiaolei Li, Shiqing Wang, Xiaotong Xue, Rui Liu, Xiaojing Bai, Pixia Gong, Chao Feng, Lijuan Cao, Tingting Wang, Yayun Ding, Junjie Jiang, Yongjing Chen, Yufang Shi, Changshun Shao
Summary: This study found that Nestin-positive mesenchymal stromal cells (T-MSCs) mainly distribute in the medulla and cortical-medulla junction of the thymus, and possess immunosuppressive ability. Additionally, T-MSCs exhibit remarkable therapeutic efficacy in acute liver injury and inflammatory bowel disease (IBD).
Article
Cell Biology
Francesca Pagani, Elisa Tratta, Patrizia Dell'Era, Manuela Cominelli, Pietro Luigi Poliani
Summary: EBF1 plays a significant role in cell fate commitment and is crucial for the expression and function of peri-endothelial cells in vessels. The study found that EBF1 is highly expressed in peri-endothelial cells, which represent bona fide pericytes and are confirmed in human placenta-derived pericytes and human brain vascular pericytes.
HISTOCHEMISTRY AND CELL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Anastasiia Mykuliak, Alma Yrjanainen, Antti-Juhana Maki, Arjen Gebraad, Ella Lampela, Minna Kaariainen, Toni-Karri Pakarinen, Pasi Kallio, Susanna Miettinen, Hanna Vuorenpaa
Summary: The vasculature is a crucial physiological element in human tissues, and the formation of perfusable vascular networks is essential for tissue modeling. This study co-cultured endothelial cells and stromal cells in a hydrogel to form three-dimensional vascular networks. Comparing mesenchymal stem/stromal cells (MSCs) derived from bone marrow and adipose tissue, it was found that these cells supported the formation of mature and interconnected microvascular networks-on-a-chip. However, the origin of MSCs influenced the phenotypes of the vascular networks, with BMSCs inducing the formation of fully perfusable microvasculature and ASCs resulting in partially perfusable microvascular networks. The study also showed that BMSCs outperformed ASCs in terms of pericytic characteristics and gene expression related to vasculature maturation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell & Tissue Engineering
Kim Olesen, Sergey Rodin, Wing Cheung Mak, Ulrika Felldin, Cecilia Osterholm, Andreas Tilevik, Karl-Henrik Grinnemo
Summary: The article introduces a new model for studying the dynamics of cardiac extracellular matrix, which combines gentle decellularization and recellularization techniques of cardiac tissue with image processing methods, enabling assessment of the impact of extracellular matrix composition on phenotype preservation during cell reseeding.
Article
Cardiac & Cardiovascular Systems
Michael M. Orlich, Rodrigo Dieguez-Hurtado, Regine Muehlfriedel, Vithiyanjali Sothilingam, Hartwig Wolburg, Cansu Ebru Oender, Pascal Woelffing, Christer Betsholtz, Konstantin Gaengel, Mathias Seeliger, Ralf H. Adams, Alfred Nordheim
Summary: This study reveals the crucial role of the transcription factor SRF in pericytes and vascular smooth muscle cells, which is essential for maintaining vascular integrity and tone.
CIRCULATION RESEARCH
(2022)
Article
Oncology
Alina Wittka, Julia Ketteler, Lars Borgards, Patrick Maier, Carsten Herskind, Verena Jendrossek, Diana Klein
Summary: In prostate cancer, inhibiting the gain of CAV1 in malignant epithelial cells and limiting the loss of stromal CAV1 are crucial for improving the response to radiotherapy. Additionally, targeting activated stromal fibroblasts by limiting fibroblast activation plays a key role in inhibiting the resistance-promoting CAV1-dependent signals of the tumor stroma.
FRONTIERS IN ONCOLOGY
(2022)
Correction
Cell Biology
Violetta Ritter, Franziska Krautter, Diana Klein, Verena Jendrossek, Justine Rudner
CELL DEATH & DISEASE
(2022)
Article
Cell Biology
Luca Hegedues, Kata D. Szuecs, Matthias Kudla, Julian Heidenreich, Verena Jendrossek, Samuel Pena-Llopis, Tamas Garay, Andras Czirok, Clemens Aigner, Till Ploenes, Silvia Vega-Rubin-de-Celis, Balazs Hegedues
Summary: This study investigated the effect of Nintedanib on SRC kinase, one of its targets, in MPM cells. The results showed that Nintedanib did not inhibit SRC activation and even increased SRC phosphorylation in some MPM cell lines. However, combination treatment with the SRC inhibitor Dasatinib reversed this effect. The study also revealed that combination treatment promoted autophagy in MPM cells, which was independent of the AKT/mTOR and ERK pathways.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Kexu Xiang, Christian Kalthoff, Corinna Muench, Verena Jendrossek, Johann Matschke
Summary: Inhibition of SLC25A1 leads to accumulation of oncometabolite D-2-HG, increasing the sensitivity of cancer cells to radiotherapy and delaying DNA damage repair. Combining SLC25A1 inhibition with PARP or DNA-PKcs inhibitors further enhances radiotherapy-induced DNA damage and delays repair, resulting in increased radiosensitivity of cancer cells.
CELL DEATH & DISEASE
(2022)
Article
Oncology
Isabell Goetting, Safa Larafa, Katharina Eul, Mikhail Kunin, Burkhard Jakob, Johann Matschke, Verena Jendrossek
Summary: Aberrant activation of AKT affects cellular antioxidant defense and radiosensitivity. TRAMPC1 cells overexpressing the clinically relevant AKT variant AKT-E17K exhibit improved antioxidant defense and increased radiation resistance. This change is associated with AKT-dependent regulation of cellular levels of reduced glutathione (GSH), both directly and indirectly. Pharmacological inhibition of AKT-dependent metabolic enzymes supporting defense against oxidative stress, such as glutathione synthase and glutathione reductase, enhances the eradication of clonogenic tumor cells, particularly those overexpressing AKT-E17K.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Sara Jennrich, Martin Pelzer, Tobias Tertel, Benjamin Koska, Melanie Vullings, Basant Kumar Thakur, Verena Jendrossek, Beate Timmermann, Bernd Giebel, Justine Rudner
Summary: Glioblastoma multiforme (GBM) is an aggressive tumor of the central nervous system with limited treatment options. Radiation therapy, specifically proton-based irradiation, shows promising results in treating GBM tumors. Extracellular vesicles (EVs) released by GBM cells can potentially serve as markers for predicting tumor response to radiotherapy.
FRONTIERS IN ONCOLOGY
(2022)
Review
Cell Biology
Jennifer Steens, Diana Klein
Summary: Stem cells have the unique ability to self-renew and differentiate into specialized cells. Adult stem cells exhibit limited differentiation capabilities determined by their tissue of origin. HOX genes, as master regulators of cell identity and fate, play a role in embryogenesis and maintaining positional identity. Understanding the regulation of HOX genes in stem cells may lead to advancements in regenerative medicine.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Oncology
Nika Guberina, Florian Wirsdoerfer, Martin Stuschke, Verena Jendrossek
Summary: The risk of overlapping pulmonary toxicity induced by thoracic radio(chemo)therapy and immune checkpoint inhibitor therapy in the treatment of patients suffering from non-small cell lung cancer (NSCLC) is a significant challenge in successful radioimmunotherapy. Factors such as patient selection, monitoring biomarkers, and early detection of lung tissue changes play crucial roles in optimizing treatment outcomes and minimizing side effects. The concurrent use of radiotherapy and immunotherapy can provide new curative options for locally advanced NSCLC patients, but careful management of adverse events, especially in the lung, is necessary.
Article
Oncology
Sophia A. Hogh-Binder, Diana Klein, Frederik Wolfsperger, Stephan M. Huber, Joerg Hennenlotter, Arnulf Stenzl, Justine Rudner
Summary: Radiotherapy is an important treatment option for prostate cancer, but cancer cells often develop resistance, limiting its effectiveness. The Bcl-2 protein family plays a role in regulating sensitivity to radiotherapy. In this study, we found that the levels of USP9x and Mcl-1 increased during prostate cancer progression. Blocking Mcl-1 or USP9x improved the response of prostate cancer cells to radiotherapy. Additionally, radiotherapy itself affected the stability of Mcl-1 protein in prostate cancer cells.
Article
Biochemistry & Molecular Biology
Teresa Bernardo, Anna Kuntze, Diana Klein, Feline Heinzelmann, Beate Timmermann, Claere von Neubeck
Summary: Surgery, radiotherapy, and chemotherapy are important treatments for cancer, but they often cause damage to normal tissue and lead to side effects. Proton beam radiotherapy (PBT) can spare normal tissue and is becoming increasingly important for anti-cancer therapies. This study compared the cellular response of human microvascular endothelial cells (HMEC-1) after irradiation with photons or protons alone and in combination with different sequences of doxorubicin (Dox). The results showed that radiation quality and longer Dox schedules significantly reduced cell survival and proliferation, increased apoptosis, and altered cell morphology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Kristina Zec, Stephanie Thiebes, Jenny Bottek, Devon Siemes, Philippa Spangenberg, Duc Viet Trieu, Nils Kirstein, Nirojah Subramaniam, Robin Christ, Diana Klein, Verena Jendrossek, Maria Loose, Florian Wagenlehner, Jadwiga Jablonska, Thilo Bracht, Barbara Sitek, Bettina Budeus, Ludger Klein-Hitpass, Dirk Theegarten, Olga Shevchuk, Daniel R. Engel
Summary: This study provides novel insights into the molecular adaptations of alveolar macrophages (AM) upon lung infection with Streptococcus pneumoniae. The results reveal a strong neutrophil-associated proteomic signature in AM, and identify CD11b as a central molecular hub influencing neutrophil recruitment, activation, and migration.
FRONTIERS IN IMMUNOLOGY
(2023)
Meeting Abstract
Immunology
C. Wenzek, P. Steinbach, F. Wirsdoerfer, K. Sutter, J. D. Boehme, R. Geffers, D. Bruder, V. Jendrossek, J. Buer, A. M. Westendorf, T. Knuschke
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)