Article
Chemistry, Multidisciplinary
Jie Deng, Deqing Lin, Xiangyu Ding, Yuan Wang, YuHan Hu, Hui Shi, Lin Chen, Bingyang Chu, Lei Lei, Chunmei Wen, Jiaqing Wang, Zhiyong Qian, Xingyi Li
Summary: This study combines an antioxidant, a non-steroidal anti-inflammatory drug, and a self-assembled peptide to form a hydrogel that reduces the production of inflammatory mediators. It demonstrates superior therapeutic efficacy in an animal model compared to clinically used drugs.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Immunology
Julian Ernesto Nicolas Gulin, Margarita Maria Catalina Bisio, Daniela Rocco, Jaime Altcheh, Maria Elisa Solana, Facundo Garcia-Bournissen
Summary: This study evaluates the efficacy of Miltefosine (MLT) as a monodrug and combined with benznidazole (BZ) for treating Trypanosoma cruzi infection. MLT showed promising results in inhibiting the parasite in both in vitro and in vivo models, with improved efficacy when combined with BZ. This study provides support for the potential use of MLT in Chagas disease treatment and the exploration of combination therapies.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Virology
Hannah Vanrusselt, Dieudonne Buh Kum, Valerio Taverniti, Cheng Liu, Abel Acosta Sanchez, Nikky Corthout, Sebastian Munck, Thomas F. Baumert, Leonid Beigelman, Lawrence M. Blatt, Julian A. Symons, Jerome Deval, Pierre Raboisson, Eloi R. Verrier, Andreas Jekle, Sandrine Vendeville, Yannick Debing
Summary: This study reports on the first non-HAP CAM-As (capsid assembly modulators) ALG-005398 and ALG-005863, providing a detailed in vitro intracellular characterization. These non-HAP CAM-As showed potent inhibition of HBV DNA production and blocked the establishment of cccDNA.
JOURNAL OF VIROLOGY
(2023)
Article
Polymer Science
Da Huang, Qiang Tang, Minglang Zou, Yongming Wang, Fang Luo, Jinyi Mu, Yuanzi Wu, Zuquan Weng, Zhenyu Lin
Summary: Inflammation is an immune response that can lead to diseases when uncontrolled. Natural products like curcumin have potential as alternative anti-inflammatory drugs, but their bioavailability is low. This study introduces PACE micelles as a promising platform for targeted and responsive anti-inflammatory therapy, demonstrating remarkable stability and significant anti-inflammatory activity.
Article
Immunology
Yue Lu, Yuqi Yang, Junhong Zhang, Hongyu Zhang, Changju Ma, Xiaojuan Tang, Jingjing Wu, Li Li, Jianan Wei, Haiming Chen, Chuanjian Lu, Ling Han
Summary: PSORI-CM02 effectively inhibits psoriatic angiogenesis by reducing oxidative status and inflammation, suppressing the expression of angiogenesis-related molecules, and inhibiting the activation of the MAPK signalling pathway.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Adrian Y. S. Lee, Joanne H. Reed, Tom P. Gordon
Summary: The diagnostic autoantibodies anti-Ro60 and anti-Ro52/TRIM21 are commonly found in systemic autoimmune disorders like SLE, often co-existing despite lacking physical linkage, signaling a convergence of failed immunological tolerance processes. Sometimes classified together as the SSA or Ro autoantibody system without clear distinction, further research is needed to explore separate detection and reporting for these antibodies and their roles in autoimmunity. Understanding their origins and pathogenic functions will provide insights for preventing and treating autoimmune disorders.
JOURNAL OF AUTOIMMUNITY
(2021)
Article
Multidisciplinary Sciences
Merve Arslan, Murat Karadag, Ebru Onal, Emine Gelinci, Gulcin Cakan-Akdogan, Sibel Kalyoncu
Summary: Single chain antibody fragments (scFvs) are widely used in diagnostics and therapeutics due to their small size and various engineering approaches. The design of the linker between the variable heavy and light chains of scFv plays a crucial role in its construction. The flexibility of the linker is determined by its length and sequence content, and flexible linkers such as glycine-serine (GS) are commonly preferred. However, repeated sequences in GS linkers can cause problems in PCR-based engineering and immunogenicity.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Weiming Xu, Yijun Niu, Xin Ai, Chengjie Xia, Ping Geng, Haiyan Zhu, Wei Zhou, Hai Huang, Xunlong Shi
Summary: This study constructed a novel liver-targeted baicalin liposome to enhance the bioavailability and antiviral ability of baicalin. The results showed that the liposomes significantly improved cellular uptake and specific distribution in the liver, leading to substantial inhibitory effects on HBV molecules. The enhanced efficacy was attributed to the interaction between hepatocyte nuclear factors and estrogen receptors.
Article
Multidisciplinary Sciences
Rachel Yamin, Andrew T. Jones, Hans-Heinrich Hoffmann, Alexandra Schafer, Kevin S. Kao, Rebecca L. Francis, Timothy P. Sheahan, Ralph S. Baric, Charles M. Rice, Jeffrey V. Ravetch, Stylianos Bournazos
Summary: Monoclonal antibodies with optimized Fc domains show superior potency in preventing and treating COVID-19 in animal disease models, reducing the dose required for protection against SARS-CoV-2 challenge and for treating pre-infected animals. Selective engagement of activating Fc receptors results in improved efficacy, highlighting the importance of Fc receptor pathways in driving antibody-mediated antiviral immunity. These findings have implications for the development of Fc-engineered monoclonal antibodies with optimal Fc-effector function against COVID-19.
Review
Cell Biology
Beate Guendel, Xinyuan Liu, Matthias Loehr, Rainer Heuchel
Summary: This review examines the current in vitro models for PDAC, emphasizing the challenges of late diagnosis and treatment resistance in improving patient outcomes. Researchers have established various in vitro and in vivo models for PDAC, with a growing focus on 3D cell culture models like spheroids and organoids.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Immunology
Carolina do Prado Servian, Monica Spadafora-Ferreira, Deborah Carolina Carvalho dos Anjos, Adriana Oliveira Guilarde, Antonio Roberto Gomes-Junior, Moara Alves Santa Barbara Borges, Leticia Carrijo Masson, Joao Marcos Maia Silva, Matheus Henrique Assis de Lima, Brenda Grazielli Nogueira Moraes, Sueli Meira Souza, Luiz Eterno Xavier, Denise Cristina Andre de Oliveira, Joao Victor Batalha-Carvalho, Ana Maria Moro, Anamelia Lorenzetti Bocca, Irmtraut Araci Hoffmann Pfrimer, Nadia Lago Costa, Valeria Christina de Rezende Feres, Fabiola Souza Fiaccadori, Menira Souza, Luiz Gustavo Gardinassi, Edison Luiz Durigon, Pedro Roosevelt Torres Romao, Soraia Attie Calil Jorge, Veronica Coelho, Viviane Fongaro Botosso, Simone Goncalves Fonseca
Summary: Infection by SARS-CoV-2 induces rapid production of IgM, IgA, and IgG antibodies directed to multiple viral antigens. Different antibody profiles were identified in patients with different clinical presentations. Longitudinal analysis showed varying antibody levels in severe and fatal COVID-19 patients.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Pharmacology & Pharmacy
Gordon T. Bolger, Kresimir Pucaj, Yvonne O. Minta, Peter Sordillo
Summary: Considerable interest has been focused on the development of curcumin as a stand-alone therapeutic or as an adjunct therapy. However, clinical studies have not provided strong evidence for its therapeutic effect. The lack of understanding of the relationship between in vitro activity, pharmacokinetics, and in vivo activity of curcumin raises questions about its relevance as a drug. By investigating curcumin's inhibition of NF-KB in cell culture and its effects in preclinical models, this review suggests that curcumin and its metabolite may play a key role in its therapeutic effects mediated at NF-KB.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Biology
Lisanne de Vor, Bruce van Dijk, Kok van Kessel, Jeffrey S. Kavanaugh, Carla de Haas, Piet C. Aerts, Marco C. Viveen, Edwin C. Boel, Ad C. Fluit, Jakub M. Kwiecinski, Gerard C. Krijger, Ruud M. Ramakers, Freek J. Beekman, Ekaterina Dadachova, Marnix G. E. H. Lam, H. Charles Vogely, Bart C. H. van der Wal, Jos A. G. van Strijp, Alexander R. Horswill, Harrie Weinans, Suzan H. M. Rooijakkers
Summary: This study demonstrates the capability of several human monoclonal antibodies to detect Staphylococcus aureus biofilms in vitro and in vivo. These antibodies target common surface components of S. aureus and can bind clinically relevant biofilm types. One of the antibodies can specifically localize to implanted catheters.
Review
Chemistry, Physical
Aleksandr Kakinen, Ibrahim Javed, Thomas P. Davis, Pu Chun Ke
Summary: Amyloid diseases are global epidemics with limited solutions for diagnosis, treatment, and prevention;
The research on amyloidosis inhibition has evolved into a new frontier in nanomedicine, offering interdisciplinary research opportunities in materials, chemistry, and biophysics;
Due to the complex and overlapping physiopathologies of amyloid diseases, there is a crucial need for appropriate in vitro and in vivo models to validate novel anti-amyloidosis nanomedicines, and the development of new animal models reflecting the behavioral, symptomatic, and cross-talk hallmarks of amyloid diseases such as Alzheimer's, Parkinson's, and type 2 diabetes.
NANOSCALE HORIZONS
(2021)
Article
Multidisciplinary Sciences
Lais Ferraz Brito Sousa, Hellen Braga Martins Oliveira, Nathan das Neves Selis, Lorena Lobo Brito Morbeck, Talita Costa Santos, Lucas Santana Coelho da Silva, Jully Chayra Santos Viana, Mariane Mares Reis, Beatriz Almeida Sampaio, Guilherme Barreto Campos, Jorge Timenetsky, Regiane Yatsuda, Lucas Miranda Marques
Summary: The study evaluated the biological activities of the BCP-DHA combination in in vitro and in vivo experimental models of antinociception and inflammation. The results showed that BCP-DHA had significant anti-inflammatory and antinociceptive effects.
SCIENTIFIC REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Fabio Valenti, Italia Falcone, Sara Ungania, Flora Desiderio, Patrizio Giacomini, Chiara Bazzichetto, Fabiana Conciatori, Enzo Gallo, Francesco Cognetti, Gennaro Ciliberto, Aldo Morrone, Antonino Guerrisi
Summary: Treatment and management of metastatic melanoma have evolved significantly in the era of personalized medicine, benefiting from immunotherapy and advancements in multi-omics analyses. While improving clinical management, precision medicine plays a crucial role in guiding treatment decisions for individual patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Fulvia Pimpinelli, Francesco Marchesi, Giulia Piaggio, Diana Giannarelli, Elena Papa, Paolo Falcucci, Martina Pontone, Simona Di Martino, Valentina Laquintana, Antonia La Malfa, Enea Gino Di Domenico, Ornella Di Bella, Gianluca Falzone, Fabrizio Ensoli, Branka Vujovic, Aldo Morrone, Gennaro Ciliberto, Andrea Mengarelli
Summary: The study assessed the safety and immunogenicity of the BNT162b2 mRNA vaccine in hematological patients, finding lower response rates in multiple myeloma patients. It suggests that these patients should continue to maintain protective measures and monitor vaccine effectiveness post-vaccination.
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2021)
Article
Medicine, Research & Experimental
Salvatore Sciacchitano, Claudia De Vitis, Michela D'Ascanio, Simonetta Giovagnoli, Chiara De Dominicis, Andrea Laghi, Paolo Anibaldi, Andrea Petrucca, Gerardo Salerno, Iolanda Santino, Rachele Amodeo, Maurizio Simmaco, Christian Napoli, Agostino Tafuri, Arianna Di Napoli, Andrea Sacconi, Valentina Salvati, Gennaro Ciliberto, Maurizio Fanciulli, Giulia Piaggio, Luisa de Latouliere, Alberto Ricci, Rita Mancini
Summary: This study found that low FT3 serum levels are associated with severe COVID-19 and may be related to the immune response. Multi-omics approach suggests that T3 is involved in the immune response in COVID-19 and coexistent hematological malignancy, and new possible T3 target genes in these patients have been identified.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Editorial Material
Medicine, Research & Experimental
Gennaro Ciliberto
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Review
Medicine, General & Internal
Eriseld Krasniqi, Maddalena Barba, Aldo Venuti, Laura Pizzuti, Federico Cappuzzo, Lorenza Landi, Silvia Carpano, Paolo Marchetti, Alice Villa, Enrico Vizza, Greta Giuliano, Marco Mazzotta, Daniele Marinelli, Sandra Gnignera, Cristina Vincenzoni, Vincenzo Stranges, Domenico Sergi, Antonio Giordano, Federica Tomao, Marcello Maugeri-Sacca, Giuseppe Sanguineti, Francesca Sofia Di Lisa, Silverio Tomao, Gennaro Ciliberto, Patrizia Vici
Summary: HPVs are linked to cervical and oropharyngeal cancers, and circulating HPV DNA serves as a potential biomarker for clinical management. Specific and minimally invasive tests can enable early detection and treatment monitoring, with innovative techniques enhancing the detection rate of serum HPV DNA.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Oncology
Luigi Fattore, Ciro Francesco Ruggiero, Domenico Liguoro, Vittorio Castaldo, Angiolina Catizone, Gennaro Ciliberto, Rita Mancini
Summary: Immunotherapy has revolutionized the treatment of metastatic melanoma by increasing long term overall survival, but the prediction of patient response is still a challenge. Liquid Biopsies (LBs) have emerged as a promising tool to predict response to immunotherapy through non-invasive biomarker measurements, and are expected to become a routine practice in guiding clinical decisions for cancer immunotherapy.
FRONTIERS IN ONCOLOGY
(2021)
Letter
Hematology
Francesco Marchesi, Fulvia Pimpinelli, Eleonora Sperandio, Elena Papa, Paolo Falcucci, Martina Pontone, Simona di Martino, Luisa de Latouliere, Giulia Orlandi, Aldo Morrone, Gennaro Ciliberto, Andrea Mengarelli
BRITISH JOURNAL OF HAEMATOLOGY
(2022)
Letter
Oncology
Francesco Marchesi, Fulvia Pimpinelli, Diana Giannarelli, Livia Ronchetti, Elena Papa, Paolo Falcucci, Martina Pontone, Enea Gino Di Domenico, Simona di Martino, Valentina Laquintana, Chiara Mandoj, Laura Conti, Iole Cordone, Antonia La Malfa, Caterina Viggiani, Daniela Renzi, Francesca Palombi, Atelda Romano, Francesco Pisani, Svitlana Gumenyuk, Ornella Di Bella, Branka Vujovic, Aldo Morrone, Gennaro Ciliberto, Fabrizio Ensoli, Andrea Mengarelli
Article
Biotechnology & Applied Microbiology
Antonella Conforti, Emanuele Marra, Fabio Palombo, Giuseppe Roscilli, Micol Rava, Valeria Fumagalli, Alessia Muzi, Mariano Maffei, Laura Luberto, Lucia Lione, Erika Salvatori, Mirco Compagnone, Eleonora Pinto, Emiliano Pavoni, Federica Bucci, Grazia Vitagliano, Daniela Stoppoloni, Maria Lucrezia Pacello, Manuela Cappelletti, Fabiana Fosca Ferrara, Emanuela D'Acunto, Valerio Chiarini, Roberto Arriga, Abraham Nyska, Pietro Di Lucia, Davide Marotta, Elisa Bono, Leonardo Giustini, Eleonora Sala, Chiara Perucchini, Jemma Paterson, Kathryn Ann Ryan, Amy-Rose Challis, Giulia Matusali, Francesca Colavita, Gianfranco Caselli, Elena Criscuolo, Nicola Clementi, Nicasio Mancini, Ruediger Gross, Alina Seidel, Lukas Wettstein, Jan Muench, Lorena Donnici, Matteo Conti, Raffaele De Francesco, Mirela Kuka, Gennaro Ciliberto, Concetta Castilletti, Maria Rosaria Capobianchi, Giuseppe Ippolito, Luca G. Guidotti, Lucio Rovati, Matteo Iannacone, Luigi Aurisicchio
Summary: This study reports the preclinical evaluation of a novel COVID-19 vaccine candidate based on the electroporation of engineered, synthetic cDNA encoding a viral antigen in the skeletal muscle. The vaccine showed strong immunogenicity and significant protection in animal models, suggesting its potential as an ideal COVID-19 vaccine candidate for clinical development.
Article
Immunology
Chiara Agrati, Serena Di Cosimo, Daniela Fenoglio, Giovanni Apolone, Fabio Ciceri, Gennaro Ciliberto, Fausto Baldanti, Massimo Costantini, Diana Giannarelli, Giuseppe Ippolito, Franco Locatelli, Alberto Mantovani, Aldo Morrone, Fabrizio Tagliavini, Antonio Uccelli, Pier Luigi Zinzani, Nicola Silvestris, Maria Rescigno
Summary: This article reviews the immune responses to vaccination in patients with malignancy, neurological and immunological disorders, proposing the VAX4FRAIL study to evaluate immune responses in this population.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Oncology
Marco Mazzotta, Laura Pizzuti, Eriseld Krasniqi, Francesca Sofia Di Lisa, Federico Cappuzzo, Lorenza Landi, Domenico Sergi, Fabio Pelle, Sonia Cappelli, Claudio Botti, Enrico Vizza, Silverio Tomao, Luca Marchetti, Giuseppe Sanguineti, Andrea Botticelli, Paolo Marchetti, Valentina Magri, Simona Pisegna, Aldo Venuti, Federica Tomao, Federica Buzzacchino, Gennaro Ciliberto, Maddalena Barba, Patrizia Vici
Summary: Vulvar cancer is a challenging clinical condition to treat, with treatment typically involving a combination of surgery, radiotherapy, and chemotherapy tailored to individual patients based on clinical and pathological features. The low incidence of the disease limits the feasibility of randomized trials, resulting in decision-making that relies heavily on evidence from observational studies and clinical practice. Therapeutic management is increasingly personalized at the individual patient level, with a trend towards consolidation of evidence sharing and discussion within a multidisciplinary framework.
Article
Immunology
Raul Pellini, Aldo Venuti, Fulvia Pimpinelli, Elva Abril, Giovanni Blandino, Flaminia Campo, Laura Conti, Armando De Virgilio, Federico De Marco, Enea Gino Di Domenico, Ornella Di Bella, Simona Di Martino, Fabrizio Ensoli, Diana Giannarelli, Chiara Mandoj, Valentina Manciocco, Paolo Marchesi, Francesco Mazzola, Silvia Moretto, Gerardo Petruzzi, Fabrizio Petrone, Barbara Pichi, Martina Pontone, Jacopo Zocchi, Antonello Vidiri, Branka Vujovic, Giulia Piaggio, Aldo Morrone, Gennaro Ciliberto
Summary: The study showed that the first dose of BNT162b2 vaccine activated immune responses in 98% of the participants. Five participants did not show an increase in antibody titre 21 days after the first dose. Higher antibody levels were detected in younger (<38 years) and normal-weight participants.
Article
Oncology
E. Krasniqi, A. Sacconi, D. Marinelli, L. Pizzuti, M. Mazzotta, D. Sergi, E. Capomolla, S. Donzelli, M. Carosi, A. Bagnato, T. Gamucci, S. Tomao, C. Natoli, P. Marchetti, A. Grassadonia, N. Tinari, M. De Tursi, E. Vizza, G. Ciliberto, L. Landi, F. Cappuzzo, M. Barba, G. Blandino, P. Vici
Summary: This study focused on the prognostic and predictive potential of miRNAs in ovarian cancer by conducting array profiling of miRNA expression levels in ovarian tissue samples. Specific dysregulated microRNA sets in tumor tissue showed promising predictive and prognostic value in ovarian cancer.
BIOMARKER RESEARCH
(2021)
Review
Cell Biology
Salvatore Sciacchitano, Andrea Sacconi, Claudia De Vitis, Giovanni Blandino, Giulia Piaggio, Valentina Salvati, Christian Napoli, Paolo Marchetti, Beatrice Salimbeni Taurelli, Flaminia Coluzzi, Monica Rocco, Andrea Vecchione, Paolo Anibaldi, Adriano Marcolongo, Gennaro Ciliberto, Rita Mancini, Carlo Capalbo
Summary: The study found that the H-Ras gene was significantly upregulated in COVID-19 patients, while K-Ras and N-Ras genes were downregulated, especially in patients with more severe disease. Comparative analysis with other datasets further confirmed the upregulation of the H-Ras gene. The H-Ras gene may participate in the host immune response to SARS-CoV-2 virus infection.
CELL DEATH DISCOVERY
(2021)
Article
Oncology
Susan Costantini, Andrea Polo, Francesca Capone, Marina Accardo, Angela Sorice, Rita Lombardi, Palmina Bagnara, Federica Zito Marino, Martina Amato, Michele Orditura, Maddalena Fratelli, Gennaro Ciliberto, Alfredo Budillon
Summary: In this study, the expression profiles of twenty-five human selenoproteins in triple negative breast cancer (TNBC) were analyzed. It was found that five selenoproteins were specifically dysregulated in TNBC. Additionally, selenoprotein S and its interacting protein valosin-containing protein (VCP/p97) were identified as mechanistically related proteins whose coordinated over-expression was associated with poor prognosis in TNBC.