Review
Cell Biology
Yue Ruan, Subao Jiang, Aytan Musayeva, Norbert Pfeiffer, Adrian Gericke
Summary: This article discusses the regulation of corneal epithelium regeneration by the limbal stem cell reservoir, the consequences of limbal stem cell deficiency caused by various factors, and the therapeutic options available for treating the condition.
Review
Cell Biology
Stefania Crippa, Ludovica Santi, Margherita Berti, Giada De Ponti, Maria Ester Bernardo
Summary: The human organism requires the production of approximately 1 trillion new blood cells per day through hematopoiesis in the bone marrow, regulated by the BM microenvironment and HSPCs. The BM niche, defined by interactions between HSPCs and non-hematopoietic cells, controls HSPC maintenance and orchestrates hematopoiesis according to the body's needs. Mesenchymal stromal cells (MSCs) play a key role in the BM niche by regulating HSPC homeostasis and impacting the outcome of hematopoietic stem cell transplantation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Biomedical
Yinbo Xiao, ChanelleA. S. McGuinness, W. Sebastian Doherty-Boyd, Manuel Salmeron-Sanchez, Hannah Donnelly, Matthew J. Dalby
Summary: Hematopoietic stem cells play a fundamental role in the generation of blood and immune cells. Although the signaling mechanisms in their bone marrow niche microenvironment have been increasingly understood, the ability to recreate these mechanisms in vitro for clinical purposes is still lacking. Recent studies have used engineering techniques to establish ex vivo bone marrow niche models, showing promising potential for research and clinical applications.
Review
Cell & Tissue Engineering
Sara Galindo, Ana de la Mata, Marina Lopez-Paniagua, Jose M. Herreras, Inmaculada Perez, Margarita Calonge, Teresa Nieto-Miguel
Summary: Mesenchymal stem cells (MSCs) have unique properties that make them effective for treating ocular surface diseases. The most commonly used administration routes for MSC treatment of Limbal Stem Cell Deficiency (LSCD) include intravenous and intraperitoneal injections, topical administration, and MSC transplantation using carrier substrata like amniotic membrane (AM). Subconjunctival injection is a minimally invasive technique that is frequently used in ophthalmology to treat corneal failure due to LSCD.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biology
Naresh Polisetti, Lyne Sharaf, Thomas Reinhard, Guenther Schlunck
Summary: This study describes an optimized method for isolating LMSC from cadaveric corneal limbal tissue, which involves collagenase digestion and flow sorting. The method yields a pure population of LMSC with superior self-renewal capacity and transdifferentiation potential, and supports the maintenance of the LEPC phenotype.
Article
Biotechnology & Applied Microbiology
Corina A. Ghebes, Jess Morhayim, Marion Kleijer, Merve Koroglu, Stefan J. Erkeland, Remco Hoogenboezem, Eric Bindels, Floris P. J. van Alphen, Maartje van den Biggelaar, Martijn A. Nolte, Bram C. J. van der Eerden, Eric Braakman, Carlijn Voermans, Jeroen van de Peppel
Summary: Recent research has shown that extracellular vesicles (EVs) secreted by adult bone marrow-derived mesenchymal stromal cells have the ability to support the ex vivo expansion of hematopoietic stem and progenitor cells (HSPCs), while those from fetal sources have a weaker effect. Protein composition analysis of EVs revealed that adult-derived EVs are rich in proteins related to oxidation-reduction processes, mitochondrial ATP synthesis, and protein folding, while fetal-derived EVs are rich in proteins linked to extracellular matrix organization, cell migration, and TGFBR signaling pathway.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Ophthalmology
Christiane Kesper, Arne Viestenz, Cornelia Wiese-Rischke, Marina Scheller, Thomas Hammer
Summary: The limbus of the eye is the location of the corneal epithelial stem cell niche. The aim of this study was to detect IRF8 in limbal epithelial stem cells and to determine its function. IRF8 was found to have an influence on the maintenance of stem cell properties in the limbus and affect the expression of Nanog.
EXPERIMENTAL EYE RESEARCH
(2022)
Article
Multidisciplinary Sciences
Masaru Mizukoshi, Masaru Kaku, Lay Thant, Kohei Kitami, Moe Arai, Isao Saito, Katsumi Uoshima
Summary: The study revealed that periodontal ligament (PDL) cells exhibit dynamic proliferative characteristics during orthodontic tooth movement-induced tissue remodeling, with proliferative cells likely undergoing further division and contributing to tissue remodeling. Additionally, proliferating cells expressed various molecular markers, indicating the lineage plasticity of PDL cells in vivo.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Sarah Y. T. Robertson, JoAnn S. Roberts, Sophie X. Deng
Summary: Limbal epithelial stem/progenitor cells (LSCs) reside in a niche with finely tuned balances of various signaling pathways, and respond to molecular signals as well as physical structure. Damage to LSCs or their niche leads to limbal stem cell deficiency (LSCD), and understanding the molecular regulation of LSCs is crucial for treating LSCD effectively.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Yuan Wang, Ryohichi Sugimura
Summary: Hematopoietic stem cells (HSCs) are promising for treating various hereditary and hematologic diseases by differentiating into mature blood cells. However, the low population of HSCs in cord blood and bone marrow has led to investigations on ex vivo HSC expansion. This review surveys the current techniques for HSC self-renewal and summarizes the effects of intrinsic and extrinsic factors on HSC expansion, as well as the challenges and opportunities in this field.
EXPERIMENTAL CELL RESEARCH
(2023)
Review
Cell Biology
Soudeh Ghafouri-Fard, Vahid Niazi, Mohammad Taheri, Abbas Basiri
Summary: Hematopoietic stem cells play a crucial role in both preserving the blood system and potentially differentiating into non-hematopoietic cells for the treatment of human disorders.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Biomedical
Hye Sung Kim, Tzu-Chieh Ho, Moshe J. Willner, Michael W. Becker, Hae-Won Kim, Kam W. Leong
Summary: In this study, we propose an ex vivo T cell expansion system that mimics natural antigen-presenting cells for adoptive cell therapy. The system utilizes microfiber scaffolds coated with dendritic cell membrane to replicate the physicochemical properties of dendritic cells, leading to greater expansion and functionality of T cells. This scalable and customizable platform holds promise for future research and application in cell therapy.
BIOACTIVE MATERIALS
(2023)
Article
Medicine, Research & Experimental
Nopmullee Tanhuad, Umnuaychoke Thongsa-Ad, Nareerat Sutjarit, Ploychompoo Yoosabai, Wittaya Panvongsa, Sirapope Wongniam, Apichart Suksamrarn, Pawinee Piyachaturawat, Usanarat Anurathapan, Suparerk Borwornpinyo, Arthit Chairoungdua, Suradej Hongeng, Kanit Bhukhai
Summary: The use of ASPP 049 has been shown to significantly increase the number of adult CD34+ cells and improve their functional properties, thus enhancing the ex vivo culture of hematopoietic stem cells.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Review
Cell & Tissue Engineering
Abdelrahman M. Elhusseiny, Mohammad Soleimani, Taher K. Eleiwa, Reem H. ElSheikh, Charles R. Frank, Morteza Naderan, Ghasem Yazdanpanah, Mark Rosenblatt, Ali R. Djalilian
Summary: The regeneration of corneal epithelium relies on limbal epithelial stem cells (LESCs) residing in the limbus, which are regulated by the limbal niche. Disruption to LESCs or their niche can lead to limbal stem cell deficiency (LSCD), impacting corneal and visual function.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Jie Yang, Guanghui Shen, Jun Cao, Jinlan Zhang, Yunqing Gu, Xinyu Zhang, Xiaoyi Jiang, Min Luo, Zhigang Lu
Summary: Membrane anchored Angptl2 shows higher activities in activating LILRB2 and expanding HSCs compared to soluble protein.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)