Article
Chemistry, Multidisciplinary
Yuxiang Hu, Hongtao Tian, Wei Chen, Yunlu Liu, Yulin Cao, Hongxin Pei, Chaochang Ming, Cunqing Shan, Xihui Chen, Zhipeng Dai, Shuhua Yang, Zengwu Shao, Shenghui Lan, Yong Liu, Wei Tong
Summary: Disuse osteoporosis is characterized by decreased bone mass caused by abnormal mechanical stimulation of bone. Piezo1 is a major mechanosensitive ion channel in bone homeostasis. Activation of Piezo1 promotes proliferation and osteogenic differentiation of bone cells, improving osteoporosis.
Review
Biochemistry & Molecular Biology
Qiang Zong, Katrin Bundkirchen, Claudia Neunaber, Sandra Noack
Summary: Bone marrow-derived mesenchymal stem cells (BMSCs) have potential for cell-based therapies, but their properties can be affected by the overweight/obesity microenvironment. This review summarizes evidence on the effects of overweight/obesity on BMSC characteristics. Existing studies show inconsistent results and the mechanisms involved are still unclear. Further research is needed to improve the functions of overweight/obesity-derived BMSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jiranuwat Sapudom, Mei ElGindi, Marc Arnoux, Nizar Drou, Anna Garcia-Sabate, Jeremy C. M. Teo
Summary: Exposure to microgravity impairs fibroblast differentiation, as indicated by reduced expression of alpha-smooth muscle actin and decreased translocation of Smad2/3 into the cell nucleus. Matrix remodeling and production are also decreased under s mu G conditions, along with transcriptomic changes in myofibroblasts. These findings suggest that fibroblast differentiation, as well as matrix production and remodeling, are impaired in 3D culture under simulated microgravity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Yu Liu, Markus Tingart, Sophie Lecouturier, Jianzhang Li, Joerg Eschweiler
Summary: This study investigated the gene expression patterns and mechanisms of adipogenic and osteogenic differentiation of bone marrow mesenchymal stem cells, identifying key genes associated with these differentiation processes. Additionally, the study provided valuable insights for further research in this area.
Article
Biochemistry & Molecular Biology
Chi Nguyen Quynh Ho, Minh Thi Tran, Chung Chinh Doan, Son Nghia Hoang, Diem Hong Tran, Long Thanh Le
Summary: Simulated microgravity affects the proliferative ability of CCL-13 cells by inhibiting cell cycle progression and inducing cytoskeleton reorganization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Zhen Cao, Yang Zhang, Shuping Wei, Xizheng Zhang, Yong Guo, Biao Han
Summary: In this study, the differential expression profiles of circRNAs and mRNAs in osteoblasts under simulated microgravity were identified, indicating their roles in cell morphology and signaling pathways. Nine core regulatory genes, including 3 circRNAs, were identified to play important roles in osteoblast differentiation. The circRNA-miRNA-mRNA network suggested that circRNAs might regulate osteoblast differentiation through the miRNA sponge mechanism.
Article
Cell Biology
Chiyuan Ma, Yue Xiong, Pei Han, Xueying Zhang, Yujing Cao, Baobei Wang, Huashan Zhao, Enkui Duan, Jian V. Zhang, Xiaohua Lei
Summary: This study demonstrates for the first time the significant impact of microgravity on hematopoietic differentiation of human pluripotent stem cells (hPSCs), and reveals the underlying mechanism for the effect of simulated microgravity on hematopoiesis development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Engineering, Aerospace
Funmilola A. Oluwafemi, Adhithiyan Neduncheran
Summary: Gravity on Earth can be modified or compensated using microgravity platforms. Products created under microgravity often have superior properties compared to their terrestrial counterparts, making them attractive for marketing. Different types of platforms, such as drop towers and space laboratories, are used for short-term and long-term experiments to study the effects of microgravity.
ADVANCES IN SPACE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Jiawen Zheng, Lanxin Zhang, Zhen Tan, Qing Zhao, Xiaoyu Wei, Yuqing Yang, Rong Li
Summary: The study revealed that Bmal1 and Per2, as core components of circadian clock genes, exhibit synergistic effects on the proliferation and differentiation of BMSCs by negatively regulating the osteogenic differentiation through the Wnt/β-catenin signaling pathway.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Yansiwei Cheng, Yuhao Zhou, Wenjun Lv, Qing Luo, Guanbin Song
Summary: Research shows that microgravity inhibits dermal fibroblastic differentiation of BMSCs by suppressing ERK/beta-catenin signaling, indicating a potential intervention target for repairing skin injuries in microgravity conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Zhao Yan, Banjun Ruan, Shan Wang, Tianshu Du, Xiaolong Shao, Guo Chen, Li Wang, Dongsheng Zhai, Shu Zhu, Zifan Lu, Xiaorui Cao
Summary: This study investigates the role of QKI in bone formation and fat accumulation in mesenchymal stem cells (MSCs). The results show that QKI suppresses osteogenic differentiation of BMSCs by downregulating the expressions of Wnt5b, Fzd7, Dvl3, and β-catenin. Additionally, QKI deficient BMSCs transplantation may provide a new strategy for the treatment of orthopedic diseases such as osteoporosis.
ARCHIVES OF MEDICAL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Longwei Hu, Yang Wang, Hongya Pan, Kathreena Kadir, Jin Wen, Siyi Li, Chenping Zhang
Summary: ARC promotes BMSC proliferation, inhibits cell apoptosis, and enhances BMSC osteogenic differentiation in vitro. In vivo, ARC can inhibit BMSC apoptosis and increase new bone formation. ARC mainly regulates BMSCs by activating the Fgf-2/PI3K/Akt pathway, providing a promising method for bone tissue engineering.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Cristina Manis, Antonio Murgia, Alessia Manca, Antonella Pantaleo, Giacomo Cao, Pierluigi Caboni
Summary: Our study using a hyphenated ion mobility mass spectrometric platform for lipid profiling of human erythrocytes expands the understanding of cellular processes and stress due to microgravity. We identified complex lipids associated with microgravity conditions, such as oxidized phosphocholines, phosphocholines bearing arachidonic in their moiety, as well as sphingomyelins and hexosyl ceramides. These findings provide insights into molecular alterations and erythrocyte lipidomics signatures associated with microgravity conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Minjin Kim, Gyeongin Jang, Kyu-Sung Kim, Jinwook Shin
Summary: Microgravity negatively regulates the survival and effector function of mast cells, as well as impairs mast cell degranulation and cytokine secretion.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yangge Du, Min Zhang, Xuejiao Liu, Zheng Li, Menglong Hu, Yueming Tian, Longwei Lv, Xiao Zhang, Yunsong Liu, Ping Zhang, Yongsheng Zhou
Summary: CDC20, in addition to its well-known role in cell cycle regulation, plays a crucial role in osteogenic commitment of BMSCs. It governs bone formation through regulating ubiquitination and degradation of p65, and knockdown of p65 rescues bone loss in Cdc20 conditional knockout mice.
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)