Article
Multidisciplinary Sciences
Shizhen Shen, Zhangjin Shen, Conghui Wang, Xiaodong Wu, Lingfang Wang, Lele Ye, Songfa Zhang, Xiaodong Cheng
Summary: In this study, a method for extracting extracellular vesicles (EVs) from epithelial ovarian cancer (EOC) tissues was optimized to investigate the effect of cryopreservation on the phenotypes, contents, and biological functions of the extracted EVs. The results showed that cryopreservation affected the yield and distribution of EVs, but not their morphology or particle mass ratio. The storage of tissues at -80°C increased both small and large EV yields, while the storage of lysates at -80°C decreased large EV yield and increased small EV yield. The cryopreserved EOC tissue retained a high percentage of proteins and miRNAs, and the extracted EVs could promote angiogenesis and invasive migration ability in human endothelial cells. This suggests that cryopreserved EOC tissue has clinical value for studying tissue-derived EVs, especially those related to EV proteins.
Review
Oncology
Akbar Lulu Marzan, Sarah Elizabeth Stewart
Summary: Pancreatic cancer is a highly lethal cancer with a 5-year survival rate of less than 10%, largely due to factors such as inadequate diagnostics, rapid metastasis, and chemoresistance. Research on extracellular vesicles in pancreatic cancer progression and chemoresistance is crucial for identifying new treatment options. Accumulating evidence suggests that extracellular vesicles may serve as biomarkers for early detection and as potential therapeutic targets or drug delivery tools for the treatment of pancreatic cancer.
Article
Cell Biology
Nasibeh Karimi, Razieh Dalirfardouei, Tomas Dias, Jan Lotvall, Cecilia Lasser
Summary: This study reveals the presence of multiple subpopulations of extracellular vesicles (EVs) carrying different tetraspanins in both plasma and serum. The sampling methods and centrifugation protocols used for blood can impact EV analysis results.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Review
Cell Biology
Goncalo J. M. Afonso, Carla Cavaleiro, Jorge Valero, Sandra I. Mota, Elisabete Ferreiro
Summary: Amyotrophic lateral sclerosis (ALS) is a severe and incurable neurodegenerative disease characterized by progressive motor neuron death. Extracellular vesicles (EVs) have been found to play a crucial role in the development of ALS and can be a potential therapeutic strategy. EVs can transport abnormal bioactive cargo and serve as biomarkers for ALS, as well as be used for therapeutic applications such as stem cell-derived exosomes.
Review
Biochemistry & Molecular Biology
Eleni-Myrto Trifylli, Anastasios G. Kriebardis, Evangelos Koustas, Nikolaos Papadopoulos, Sofia Vasileiadi, Sotirios P. Fortis, Vassilis L. Tzounakas, Alkmini T. Anastasiadi, Panagiotis Sarantis, Effie G. Papageorgiou, Ariadne Tsagarakis, Georgios Aloizos, Spilios Manolakopoulos, Melanie Deutsch
Summary: This review provides an overview of the emerging role of extracellular vesicles in cholangiocarcinogenesis and their potential as diagnostic and therapeutic tools.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Elisabeta Badila, Cristina Japie, Ana-Maria Vrabie, Adrian Badila, Adriana Georgescu
Summary: Cardiovascular disease and cancer remain significant causes of morbidity and mortality globally. The prevention and treatment of heart disease in cancer patients present ongoing challenges for improving their quality of life and survival. Limited progress has been made in early detection of cardiovascular disease in cancer patients, highlighting the importance of understanding the molecular mechanisms and disease-specific biomarkers in the communication between cancer and cardiovascular disease. Research on extracellular vesicles (EVs) as mediators of this cross talk is still in its early stages. Investigating EV-based biomarkers and therapies may provide insights into responsible mechanisms, early detection of cardiovascular damage, and new therapeutic approaches. The ultimate goal is to reduce the burden of cardiovascular diseases in oncological patients receiving anticancer drugs or radiotherapy.
Review
Biochemistry & Molecular Biology
Ines Dinis Aires, Teresa Ribeiro-Rodrigues, Raquel Boia, Magda Ferreira-Rodrigues, Henrique Girao, Antonio Francisco Ambrosio, Ana Raquel Santiago
Summary: Microglial cells are important for maintaining neuronal homeostasis in the central nervous system, while chronic neuroinflammation plays a role in neurodegenerative diseases. Extracellular vesicles, such as apoptotic bodies, microvesicles, and exosomes, are involved in intercellular communication and may regulate microglial cell function. Understanding the role of microglial extracellular vesicles could provide insights into the mechanisms underlying neurodegeneration.
Review
Immunology
Chang Tian, Ke Wang, Min Zhao, Shan Cong, Xin Di, Ranwei Li
Summary: Sepsis, a life-threatening condition caused by a dysregulated host response to infection, is a major cause of mortality worldwide. Early diagnosis and effective treatment of sepsis remain challenging due to its rapid progression and heterogeneity among individuals. This review highlights the role of extracellular vesicles (EVs) in sepsis, including their impact on pathological processes, organ dysfunction, and potential applications such as early diagnosis, dynamic monitoring, precision therapies, and vaccine platforms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Oncology
Tamkin Ahmadzada, Abhishek Vijayan, Fatemeh Vafaee, Ali Azimi, Glen Reid, Stephen Clarke, Steven Kao, Georges E. Grau, Elham Hosseini-Beheshti
Summary: In this study, we characterized different populations of extracellular vesicles (EVs) derived from pleural mesothelioma cell lines and identified unique cancer-specific proteomes and oncogenic cargo in each subtype. We also established a comprehensive proteomic database of cancer-associated proteins in EVs, which could serve as new targets for future biomarker studies in pleural mesothelioma.
Article
Neurosciences
Arada Vinaiphat, Siu Kwan Sze
Summary: Extracellular vesicles (EVs) are lipid bilayer particles released by almost every cell type, carrying proteins, RNAs, etc. that reflect the cellular state and participate in intercellular communication. Recent studies have shown that EVs play a role in neuroprotection and contribute to the onset and progression of neurodegenerative diseases. Therefore, studying EV proteome can be used for the diagnosis and treatment of neurodegenerative diseases.
EXPERIMENTAL NEUROLOGY
(2022)
Review
Biochemistry & Molecular Biology
Katarzyna Horodecka, Markus Duchler
Summary: The establishment of CRISPR/Cas9 technology has revolutionized gene editing and therapeutic interventions, but challenges remain in effectively delivering components to living organisms. Utilizing extracellular vesicles as delivery devices may help overcome limitations and improve the clinical applications of this technology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Chemistry, Multidisciplinary
Yujia Hao, Haoyue Song, Zilan Zhou, Xiaohang Chen, Huifei Li, Yuan Zhang, Jie Wang, Xiuyun Ren, Xing Wang
Summary: Extracellular vesicles (EVs) are important vehicles for intercellular communication, with different physiological and pathological effects, and can be used as biological therapeutic agents. In some cases, the release of EVs can exacerbate disease progression, while inhibition may delay disease development.
JOURNAL OF CONTROLLED RELEASE
(2021)
Review
Biochemistry & Molecular Biology
Marc Liebana-Jordan, Bruno Brotons, Juan Manuel Falcon-Perez, Esperanza Gonzalez
Summary: Extracellular vesicles play a crucial role in intercellular communication, especially in fungi, by maintaining cell structure, defending against the environment, and potentially impacting host interactions and disease outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Delia Gagliardi, Nereo Bresolin, Giacomo Pietro Comi, Stefania Corti
Summary: Extracellular vesicles (EVs) serve as small reservoirs of molecules and play important roles in cell-to-cell communication, with potential implications in disease pathogenesis and therapy. They are not only being studied as diagnostic and prognostic biomarkers but also as valuable tools for understanding disease mechanisms and exploring therapeutic applications.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Asit Kumar, Lina Zhou, Kaining Zhi, Babatunde Raji, Shelby Pernell, Erene Tadrous, Sunitha Kodidela, Anantha Nookala, Harry Kochat, Santosh Kumar
Summary: This review provides an overview of biomaterials as potential drug delivery systems for neurogenerative diseases, discussing the desired properties of effective drug delivery systems and the prospects and limitations of using extracellular vesicles (EVs) as drug delivery systems for targeted delivery with limited immunogenicity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Donato Melchionda, Tommaso Martino, Elena Carapelle, Alessandra Lalla, Daniela Cologno, Carlo Avolio
NUTRITIONAL NEUROSCIENCE
(2018)
Article
Behavioral Sciences
G. d'Orsi, T. Martino, A. Lalla, E. Carapelle, M. T. Di Claudio, C. Avolio, G. Pustorino, A. N. Polito, A. La Neve, T. Francavilla, G. Boero, G. Pontrelli, S. Interno, S. Patane, M. Germano, L. La Selva, V. Sciruicchio, V. Durante, R. Scarpello, M. Elia
EPILEPSY & BEHAVIOR
(2018)
Article
Clinical Neurology
Angelo Fabio Gigante, Artor Niccoli Asabella, Giovanni Iliceto, Tommaso Martino, Cristina Ferrari, Giovanni Defazio, Giuseppe Rubini
NEUROLOGICAL SCIENCES
(2018)
Article
Clinical Neurology
Marina Trivisano, Nicola Pietrafusa, Alessandra Terracciano, Carla Marini, Davide Mei, Francesca Darra, Patrizia Accorsi, Domenica Battaglia, Lorella Caffi, Maria P. Canevini, Simona Cappelletti, Elisabetta Cesaroni, Luca de Palma, Paola Costa, Raffaella Cusmai, Lucio Giordano, Annarita Ferrari, Elena Freri, Lucia Fusco, Tiziana Granata, Tommaso Martino, Massimo Mastrangelo, Stefania M. Bova, Lucio Parmeggiani, Francesca Ragona, Federico Sicca, Pasquale Striano, Luigi M. Specchio, Ilaria Tondo, Elena Zambrelli, Nelia Zamponi, Caterina Zanus, Clementina Boniver, Marilena Vecchi, Carlo Avolio, Bernardo Dalla Bernardina, Enrico Bertini, Renzo Guerrini, Federico Vigevano, Nicola Specchio
Article
Endocrinology & Metabolism
Giovanni De Pergola, Tommaso Martino, Roberta Zupo, Domenico Caccavo, Claudio Pecorella, Silvia Paradiso, Franco Silvestris, Vincenzo Triggiani
ENDOCRINE METABOLIC & IMMUNE DISORDERS-DRUG TARGETS
(2019)
Letter
Genetics & Heredity
Francesca Bisulli, Lorenzo Muccioli, Giuseppe d'Orsi, Laura Canafoglia, Elena Freri, Laura Licchetta, Barbara Mostacci, Patrizia Riguzzi, Federica Pondrelli, Carlo Avolio, Tommaso Martino, Roberto Michelucci, Paolo Tinuper
ORPHANET JOURNAL OF RARE DISEASES
(2019)
Article
Clinical Neurology
Laura Canafoglia, Giuseppina Barbella, Edoardo Ferlazzo, Pasquale Striano, Adriana Magaudda, Giuseppe d'Orsi, Tommaso Martino, Carlo Avolio, Umberto Aguglia, Chiara Sueri, Loretta Giuliano, Vito Sofia, Federica Zibordi, Francesca Ragona, Elena Freri, Cinzia Costa, Elena Nardi Cesarini, Martina Fanella, Davide Rossi Sebastiano, Patrizia Riguzzi, Antonio Gambardella, Carlo Di Bonaventura, Roberto Michelucci, Tiziana Granata, Francesca Bisulli, Laura Licchetta, Paolo Tinuper, Francesca Beccaria, Elisa Visani, Silvana Franceschetti
Article
Clinical Neurology
Angelo Antonini, Giovanni Abbruzzese, Alfredo Berardelli, Nicola Modugno, Italo Stroppa, Filippo Tamma, Mariachiara Sensi, Francesca Mancini, Giovanni Cossu, Alessandro Stefani, Nicola Tambasco, Alessandro Tessitore, Giovanni Fabbrini, Francesco E. Pontieri, Paolo Solla, Anna Rita Bentivoglio, Cristoforo Comi, Brigida Minafra, Giulio Riboldazzi, Donato Melchionda, Tommaso Martino, Leonardo Lopiano
JOURNAL OF NEURAL TRANSMISSION
(2020)
Correction
Clinical Neurology
Angelo Antonini, Giovanni Abbruzzese, Alfredo Berardelli, Nicola Modugno, Italo Stroppa, Filippo Tamma, Mariachiara Sensi, Francesca Mancini, Giovanni Cossu, Alessandro Stefani, Nicola Tambasco, Alessandro Tessitore, Giovanni Fabbrini, Francesco E. Pontieri, Paolo Solla, Anna Rita Bentivoglio, Cristoforo Comi, Brigida Minafra, Giulio Riboldazzi, Donato Melchionda, Tommaso Martino, Leonardo Lopiano
JOURNAL OF NEURAL TRANSMISSION
(2021)
Review
Clinical Neurology
Sara Gasparini, Sabrina Neri, Francesco Brigo, Vittoria Cianci, Anna Mammi, Angelo Pascarella, Lucia Manzo, Paolo Benna, Pasquale Striano, Tommaso Martino, Michele Romoli, Lorenzo Muccioli, Annacarmen Nilo, Mariarosaria Valente, Claudia Cagnetti, Angelo Labate, Antonio Gambardella, Francesco Pisani, Sara Casciato, Giancarlo Di Gennaro, Vincenzo Belcastro, Umberto Aguglia, Edoardo Ferlazzo
Summary: This study systematically reviewed the risk factors for CVT-LS and found that symptomatic seizures, stupor/coma, focal neurologic signs, hemorrhagic component, and superior sagittal sinus involvement were the most relevant predictors of CVT-LS.
NEUROLOGICAL SCIENCES
(2022)
Editorial Material
Acoustics
Tommaso Martino
JOURNAL OF CLINICAL ULTRASOUND
(2023)
Meeting Abstract
Clinical Neurology
L. Muccioli, F. Pondrelli, A. Farolfi, G. d'Orsi, E. Freri, L. Canafoglia, F. Toni, L. Licchetta, T. Martino, G. Marotta, S. Fanti, R. Michelucci, P. Tinuper, F. Bisulli
Article
Clinical Neurology
Giuseppe d'Orsi, Tommaso Martino, Alessandra Lalla, Maria Teresa Di Claudio, Elena Carapelle, Carlo Avolio
EPILEPTIC DISORDERS
(2018)
Meeting Abstract
Clinical Neurology
A. L. Neve, G. Boero, G. d'Orsi, V. Durante, M. Plantamura, S. Interno, R. Scarpello, A. Lalla, T. Martino, C. Avolio, M. Trojano