Article
Biochemistry & Molecular Biology
Ping Zhang, Yanjie Wang, Jingyu Zhang, Tie Hong
Summary: This study investigates the anti-pseudoallergy effect of allantoin (AT) and its underlying mechanism. The results demonstrate that AT can inhibit degranulation and improve pathological changes induced by Compound 48/80. Additionally, AT helps restore body temperature decrease caused by Compound 48/80.
Article
Biochemistry & Molecular Biology
Zhuofan Li, Ki-Hye Kim, Noopur Bhatnagar, Bo Ryoung Park, Subbiah Jeeva, Yu-Jin Jung, Jannatul Raha, Sang-Moo Kang, Xinyuan Chen
Summary: This study presents a physical radiofrequency adjuvant (RFA) that can conveniently and effectively enhance the immunogenicity and efficacy of H5N1 vaccine. The RFA treatment on the skin induces thermal stress to enhance immune responses and does not cause significant adverse reactions. The RFA significantly increases antibody titers, reduces viral titers, body weight loss, and improves survival rates in murine models. It has no gender difference in its effects.
Article
Physiology
B. Malique Jones, Gerald C. Mingin, Nathan R. Tykocki
Summary: Mast cells and degranulation of inflammatory mediators contribute to lower urinary tract symptoms. This study found that the mast cell stimulator compound 48/80 alters urinary bladder smooth muscle contractility via urothelium-dependent inflammatory pathways, independent of mast cells. These findings suggest that compound 48/80 could be used to manage inflammatory nonneurogenic bladder hyperactivity.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Pragya Saxena, Eli Broemer, Gerald M. Herrera, Gerald C. Mingin, Sara Roccabianca, Nathan R. Tykocki
Summary: A balance between stiffness and compliance is important for bladder function, but changes in bladder wall properties occur in many bladder pathologies. These changes are associated with the release of secretagogues that activate mast cells and cause the release of inflammatory mediators. However, the effects of a specific mast cell activator, Compound 48/80, on bladder function were found to be independent of mast cells. Compound 48/80 increased bladder compliance and excitability through activation of Mrgprb2 receptors located outside of mast cells, suggesting that targeting Mrgprb2 could be a potential treatment for lower urinary tract dysfunction.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Multidisciplinary
Randall Toy, M. Cole Keenum, Pallab Pradhan, Katelynn Phang, Patrick Chen, Chinwendu Chukwu, Lily Anh H. Nguyen, Jiaying Liu, Sambhav Jain, Gabrielle Kozlowski, Justin Hosten, Mehul S. Suthar, Krishnendu Roy
Summary: The study suggests that simultaneous engagement of small molecule adjuvant R848 and RNA adjuvant PUUC can induce robust cellular immune responses in both mice and human cells, enhancing the effectiveness of flu vaccines.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Multidisciplinary Sciences
Lucie Loyal, Julian Braun, Larissa Henze, Beate Kruse, Manuela Dingeldey, Ulf Reimer, Florian Kern, Tatjana Schwarz, Maike Mangold, Clara Unger, Friederike Dorfler, Shirin Kadler, Jennifer Rosowski, Kubrah Gurcan, Zehra Uyar-Aydin, Marco Frentsch, Florian Kurth, Karsten Schnatbaum, Maren Eckey, Stefan Hippenstiel, Andreas Hocke, Marcel A. Muller, Birgit Sawitzki, Stefan Miltenyi, Friedemann Paul, Marcus A. Mall, Holger Wenschuh, Sebastian Voigt, Christian Drosten, Roland Lauster, Nils Lachman, Leif-Erik Sander, Victor M. Corman, Jobst Rohmel, Lil Meyer-Arndt, Andreas Thiel, Claudia Giesecke-Thiel
Summary: The study demonstrates that preexisting spike-cross-reactive T cells play a functional role in the immune response to SARS-CoV-2 infection and vaccination. Cross-reactive immunity may explain the rapid induction of immunity after primary SARS-CoV-2 immunization and the high rate of asymptomatic or mild COVID-19 cases.
Editorial Material
Immunology
Katherine Kedzierska, Thi H. O. Nguyen
Summary: The study by Wherry and colleagues examines how anti-PD-1 immunotherapy affects influenza vaccination outcomes in cancer patients, specifically looking at its impact on seroconversion and quantitative and qualitative aspects of antibodies and follicular T helper cell responses.
Review
Pharmacology & Pharmacy
Iara Maira de Oliveira Viana, Sabrina Roussel, Joan Defrene, Eliana Martins Lima, Frederic Barabe, Nicolas Bertrand
Summary: Significant progress has been made in the field of nanomedicine, allowing for more efficient drug delivery and recognition of therapeutic effects through a collaboration of engineering and medicine. Successes in gene silencing methods beyond oncology have paved the way for new therapies. The global pandemic has highlighted the potential of mRNA vaccines, emphasizing the need for rational design of therapies and vaccines in the future.
ACTA PHARMACEUTICA SINICA B
(2021)
Review
Biochemistry & Molecular Biology
Kazuki Nagata, Chiharu Nishiyama
Summary: Mast cells play critical roles in immune responses as initiators and suppressors, with IL-10 serving as a key anti-inflammatory cytokine in regulating their functions. IL-10 exerts diverse effects on mast cells in vivo and in vitro, influencing tissue homeostasis and immune-related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Lisa Beuckelaere, Maarten Haspeslagh, Evelien Biebaut, Filip Boyen, Freddy Haesebrouck, Roman Krejci, Evelyne Meyer, David Gleerup, Ward De Spiegelaere, Bert Devriendt, Dominiek Maes
Summary: This study investigated the effects of infection with, and vaccination against Mycoplasma hyopneumoniae on the innate and adaptive immune responses in pigs. Results showed that the two commercial bacterins induced different immune responses, the adjuvant alone reduced anti-inflammatory innate immune responses, and both vaccines had different efficacy in reducing lung lesions and M. hyopneumoniae DNA load.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Louise Madeleine Ince, Coline Barnoud, Lydia Kay Lutes, Robert Pick, Chen Wang, Flore Sinturel, Chien-Sin Chen, Alba de Juan, Jasmin Weber, Stephan J. Holtkamp, Sophia Martina Hergenhan, Jennifer Geddes-McAlister, Stefan Ebner, Paola Fontannaz, Benjamin Meyer, Maria Vono, Stephane Jemelin, Charna Dibner, Claire-Anne Siegrist, Felix Meissner, Frederik Graw, Christoph Scheiermann
Summary: The study shows that circadian rhythms affect immune responses, including responses to vaccines. Dendritic cells migrate in a time-of-day-dependent manner, altering interactions with T cells and influencing vaccine responses. The findings provide a mechanistic understanding of the development and maintenance of an adaptive immune response under circadian control, offering a strategy for optimizing vaccination regimes based on time-of-day.
NATURE COMMUNICATIONS
(2023)
Review
Immunology
Mariela Jimenez, Daniel Cervantes-Garcia, Laura E. Cordova-Davalos, Marian Jesabel Perez-Rodriguez, Claudia Gonzalez-Espinosa, Eva Salinas
Summary: Mast cells are immune cells strategically located in tissues close to the external environment, playing a key role in the host defense against invading pathogens. They release biologically active mediators to induce inflammation, promote antimicrobial activities, and shape innate and adaptive immune responses.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Kaige Chen, Zheng Zhang, Jiachen Zhang, Ziyuan Fang, Wang Dong, Yucai Wang, Min Li
Summary: This study explores a new antigen-caged adjuvant strategy, in which a controlled bio-mineralization method is used to prepare antigen-caged metal adjuvants. The results demonstrate that this strategy can enhance the adjuvanticity of aluminum adjuvants and promote the internalization of antigen and adjuvant by antigen-presenting cells, leading to robust humoral and cellular immune responses.
Review
Pharmacology & Pharmacy
Ben S. Ou, Olivia M. Saouaf, Julie Baillet, Eric A. Appel
Summary: The immune system plays a crucial role in regulating and protecting human health. Sustained release technologies have shown potential in enhancing adaptive immune responses by prolonging exposure to immunogenic signals.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Editorial Material
Biochemistry & Molecular Biology
Sophie A. Valkenburg, Leo L. M. Poon
Summary: The antibody landscape after H3N2 vaccination is influenced by recent infections, and studying immune dynamics can help inform influenza vaccination strategies.
Article
Immunology
Tarfa Altorki, Werner Muller, Andrew Brass, Sheena Cruickshank
Summary: The study found that the lack of functional beta(2) integrin significantly reduces dendritic cell migration to the site of infection during nematode worm infection, but not to the lymph nodes. However, the absence of functional beta(2) integrin did not negatively impact T cell activation in response to the infection.
Article
Immunology
Jan Dudeck, Johanna Kotrba, Roland Immler, Aaron Hoffmann, Martin Voss, Vasileia Ismini Alexaki, Lorena Morton, Stephan Rene Jahn, Konstantinos Katsoulis-Dimitriou, Simon Winzer, Georg Kollias, Thomas Fischer, Sergei A. Nedospasov, Ildiko Rita Dunay, Triantafyllos Chavakis, Andreas J. Mueller, Burkhart Schraven, Markus Sperandio, Anne Dudeck
Summary: Mast cell-derived tumor necrosis factor is crucial for neutrophil extravasation by promoting intraluminal crawling and provides a potential target for therapeutic approaches.
Review
Biochemistry & Molecular Biology
Martin Voss, Johanna Kotrba, Evelyn Gaffal, Konstantinos Katsoulis-Dimitriou, Anne Dudeck
Summary: Mast cells are key effector cells in immediate-type allergic reactions, but they also play an important role in the first-line defense against pathogens, although this can lead to detrimental overshooting responses and exacerbation of inflammatory disorders. In the skin, mast cells have both beneficial functions in maintaining skin barrier integrity and host defense mechanisms, as well as causing inflammatory skin disorders, with their IgE-independent activation pathways playing a crucial role in sustained chronic skin inflammation and disease exacerbation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Anna Pascual-Reguant, Ralf Koehler, Ronja Mothes, Sandy Bauherr, Daniela C. Hernandez, Ralf Uecker, Karolin Holzwarth, Katja Kotsch, Maximilian Seidl, Lars Philipsen, Werner Mueller, Chiara Romagnani, Raluca Niesner, Anja E. Hauser
Summary: ILCs are essential regulators of biological processes. The study combines multiplexed imaging and computational pipelines to identify tonsillar IRF4 (+) ILC3s and conserved stromal landmarks for ILC localization, offering a platform for future ILC research.
NATURE COMMUNICATIONS
(2021)
Article
Physics, Multidisciplinary
Eva-Maria Brinkmann, Klaus Brinker, Silvia Rueberg, Werner Mueller
Summary: This study addresses the limitations of fluorescence microscopy images caused by the limited dynamic range of the camera and noise. By introducing a variational method that considers Poisson statistics and edge-preserving denoising, high dynamic range imaging in fluorescence microscopy is achieved successfully.
JOURNAL OF PHYSICS COMMUNICATIONS
(2021)
Correction
Genetics & Heredity
Marc Janier, Mohammed Almadjub, Olivier Beuf, Claire Billotey, Radu Bolbos, Laurence Canaple, Emmanuel Chereul, Denis Grenier, Bassem Hiba, Christophe Odet, Jean-Baptiste Langlois, Carole Lartizien, Luc Magnier, Wilfried Ma, Tarik Zouagui, Mark Lathrop, Ivo Gut, Anne Puech, Yann Herault, Veronique Blanquet, Veronique Brault, Jacques Samarut, Denise Aubert, Michela Plateroti, Nadia Rosenthal, Mumna Al Banchaabouchi, Janice Carter, Olga Ermakova, Karin Gale, Jose Gonzalez, Emerald Perlas, Jeanette Rientjes, Annett Spanner, Anne-Cecile Trillat, Glauco Tocchini-Valentini, Silvia Mandillo, Elisabetta Golini, Daniela Marazziti, Giancarlo Deidda, Nicoletta Rossi, Rafaele Matteoni, Marcello Raspa, Ferdinando Scavizzi, Mariano Barbacid, Marivi Victoria Campuzano, Pierre Dubus, Lucia Perez Gallego, Carmen Guerra Gonzalez, Rudi Balling, Ursula Frischmann, Hansjorg Hauser, Andreas Lengeling, Werner Muller, Bastian Pasche, Martin Hrabe de Angelis, Wolfgang Wurst, Koichiro Abe, Johannes Beckers, Dirk H. Busch, Claudia Dalke, Valerie Gailus Durner, Ralf Elvert, Tobias J. Franz, Helmut Fuchs, Jochen Graw, Sabine M. Holter, Thilo Jakob, Svetoslav Kalaydjiev, Magdalena Kallnik, Martina Klempt, Martin Klingenspor, Thomas Klopstock, Christoph Lengger, Leticia Quintanilla Martinez, Vera Pedersen, Oliver Puk, Ilka Schneider, Daniela Vogt Weisenhorn, Sabine Wagner, Eckhard Wolf, Pierre Chambon, Johan Auwerx, Dalila Ali-Hadji, Marie-France Champy, Nelly Frossard, Jean-Jacques Helwig, Manuel Mark, Laurent Monassier, Maria Cristina Antal, Mariette Barthelmebs, Agnes Bloch-Zupan, Andre Constantinesco, Pascal Doll, Lahcen El Fertak, Francoise Gofflot, Laurent Juillard, Wojciech Krezel, Hamid Meziane, Abdel-Mouttalib Ouagazzal, Francoise Pons, Raymond Romand, Mohammed Selloum, Tania Sorg, Marius Teletin, Laurent Vasseur, Olivia Wendling, Philip Avner, Sophie Chantalat, Urban Lendahl, Johannes Wilbertz, Kay Davies, Peter Oliver, Steve Brown, Michael Cheeseman, Emma Coghill, Roger Cox, Liz Bentley, Simon Ferguson, Hilary Gates, Georgios Gkoutos, Eain Green, John Hancock, Tertius Hough, Robert Johnson, Heena Lad, Ann-Marie Mallon, Pat Nolan, Andy Parker, Julie Quarterman, Adrian Smith, Mandy Studley, Aadya Shukla, Hilda Tateossian, Valter Tucci, Joseph Weekes, Ian Jackson, Sally Cross, Duncan Davidson, Alan Hart, Angus Murray, Anton Berns, Marco Breuer, Evert van Garderen, Jos Jonkers, Ji Ying Song, Martin van der Valk, Ludwig Neyses, Elizabeth Cartwright, Delvac Oceandy, Michael Emerson, Walter Wahli, Maria Belen Delgado, Batrice Desvergne, Liliane Michalik, Elodie Bedu, Denis Duboule, Marie Kmita, Jozsef Zakany, Animage, Allan Bradley, Madhuri Warren
Article
Multidisciplinary Sciences
Ali Kinkhabwala, Christoph Herbel, Jennifer Pankratz, Dmytro A. Yushchenko, Silvia Rueberg, Paurush Praveen, Sandy Reiss, Federico Carlos Rodriguez, Daniel Schaefer, Jutta Kollet, Vera Dittmer, Manuel Martinez-Osuna, Lara Minnerup, Claudia Reinhard, Andrzej Dzionek, Thomas Dino Rockel, Stefan Borbe, Martin Buescher, Juergen Krieg, Michel Nederlof, Melanie Jungblut, Dominik Eckardt, Olaf Hardt, Christian Dose, Eik Schumann, Ralf-Peter Peters, Stefan Miltenyi, Jurgen Schmitz, Werner Mueller, Andreas Bosio
Summary: MICS technology is a new high-content microscopy technique that allows immunofluorescent imaging of hundreds of protein targets at subcellular resolution. It can identify potential targets for immune therapy and has wide applicability in personalized medicine.
SCIENTIFIC REPORTS
(2022)
Article
Developmental Biology
Jennifer Stables, Emma K. Green, Anuj Sehgal, Omkar L. Patkar, Sahar Keshvari, Isis Taylor, Maisie E. Ashcroft, Kathleen Grabert, Evi Wollscheid-Lengeling, Stefan Szymkowiak, Barry W. McColl, Antony Adamson, Neil E. Humphreys, Werner Mueller, Hana Starobova, Irina Vetter, Sepideh Kiani Shabestari, Matthew M. Blurton-Jones, Kim M. Summers, Katharine M. Irvine, Clare Pridans, David A. Hume
Summary: Amino acid substitutions in the kinase domain of the human CSF1R gene are associated with ALSP. A disease-associated mutation was created in the mouse Csf1r locus to model the human disease. This mutation affected the production of tissue macrophages and microglial cells in the mouse brain.
Article
Multidisciplinary Sciences
Stephanie Marciniak, Christina M. Bergey, Ana Maria Silva, Agata Haluszko, Miroslaw Furmanek, Barbara Veselka, Petr Veleminsky, Giuseppe Vercellotti, Joachim Wahl, Gunita Zarina, Cristina Longhi, Jan Kolar, Rafael Garrido-Pena, Raul Flores-Fernandez, Ana M. Herrero-Corral, Angela Simalcsik, Werner Mueller, Alison Sheridan, Zydrune Miliauskiene, Rimantas Jankauskas, Vyacheslav Moiseyev, Kitti Kohler, Agnes Kiraly, Beatriz Gamarra, Olivia Cheronet, Vajk Szeverenyi, Viktoria Kiss, Tamas Szeniczey, Krisztian Kiss, Zsuzsanna K. Zoffmann, Judit Koos, Magdolna Hellebrandt, Robert M. Maier, Laszlo Domboroczki, Cristian Virag, Mario Novak, David Reich, Tamas Hajdu, Noreen von Cramon-Taubadel, Ron Pinhasi, George H. Perry
Summary: The onset of agriculture around 12,000 years ago had a significant impact on human culture, biology, and health. This study examines the variation in human stature as a proxy for health across a transition to agriculture, and finds that individuals from the Neolithic period were shorter than those from the Upper Paleolithic and Mesolithic. However, the stature gradually increased across subsequent ages. These findings highlight the potential of using different datasets to explore health proxies in prehistory.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Stamatia Papoutsopoulou, Lorna Morris, Andrew Bayliff, Thomas Mair, Hazel England, Massimiliano Stagi, Francois Bergey, Mohammad Tauqeer Alam, Raheleh Sheibani-Tezerji, Philip Rosenstiel, Werner Muller, Vitor A. P. Martins Dos Santos, Barry J. Campbell
Summary: The NF kappa B transcription factors play a crucial role in innate immune responses and their dysregulation is associated with inflammatory diseases. In this study, the introduction of human RelA protein into mouse cells was shown to impact murine gene transcription, affecting both NF kappa B and non-NF kappa B target genes, and interfering with interferon and cytokine signaling pathways.
Article
Gastroenterology & Hepatology
Stamatia Papoutsopoulou, Joseph Tang, Ahmed H. Elramli, Jonathan M. Williams, Nitika Gupta, Felix I. Ikuomola, Raheleh Sheibani-Tezerji, Mohammad T. Alam, Juan R. Hernandez-Fernaud, Jorge H. Caamano, Chris S. Probert, Werner Muller, Carrie A. Duckworth, D. Mark Pritchard
Summary: NF-KB signaling pathway plays important roles in regulating cell function and immune response. In this study, we identified B cell defect and dysregulation of immunoglobulin levels in the small intestinal mucosa of Nfkb2-/- mice using transcriptomic and proteomic analysis. NF-KB2/p52 deficiency not only increases resistance to LPS-induced cell apoptosis in the small intestine, but also regulates the plasma cell population and immunoglobulin levels within the gut.
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2022)
Article
Microbiology
Calum Bridson, Latha Vellaniparambil, Rachel E. Antwis, Werner Mueller, R. Tucker Gilman, Jennifer K. Rowntree
Summary: The gut microbiota of social bees, dominated by a set of core taxa, shows variation in relative abundance across different regions and is influenced by colony membership and genetic diversity. Colony membership is a major factor affecting the variation in microbiota, while individual genetic diversity does not have a significant impact.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Immunology
Eirini Kalliara, Malgorzata Kardynska, James Bagnall, David G. Spiller, Werner Muller, Dominik Ruckerl, Jaroslaw Smieja, Subhra K. Biswas, Pawel Paszek
Summary: In this study, the researchers investigated interferon-induced JAK-STAT signaling in innate immune macrophages using live-cell confocal microscopy and mathematical modeling. They found that transient exposure to IFN-γ stimulation leads to long-term desensitization of STAT1 signaling and gene expression responses. The study also revealed that IFN-α/β 1 and IFN-γ elicit different levels of desensitization, with cells that are refractory to IFN-α/β 1 being sensitive to IFN-γ, but not vice versa.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemical Research Methods
Ellen M. Quardokus, Diane C. Saunders, Elizabeth McDonough, John W. Hickey, Christopher Werlein, Christine Surrette, Presha Rajbhandari, Anna Martinez Casals, Hua Tian, Lisa Lowery, Elizabeth K. Neumann, Frida Bjoerklund, Taruna V. Neelakantan, Josh Croteau, Anne E. Wiblin, Jeremy Fisher, April J. Livengood, Karen G. Dowell, Jonathan C. Silverstein, Jeffrey M. Spraggins, Gloria S. Pryhuber, Gail Deutsch, Fiona Ginty, Garry P. Nolan, Simon Melov, Danny Jonigk, Michael A. Caldwell, Ioannis S. Vlachos, Werner Muller, Nils Gehlenborg, Brent R. Stockwell, Emma Lundberg, Michael P. Snyder, Ronald N. Germain, Jeannie M. Camarillo, Neil L. Kelleher, Katy Boerner, Andrea J. Radtke
Summary: Organ Mapping Antibody Panels are standardized antibody panels created by the community for multiplexed spatial imaging. This imaging technique allows detailed characterization of molecular and cellular organization in tissues. Although high-parameter data collection is now possible (>60 targets), constructing the necessary antibody panels requires expertise and capital. Organ Mapping Antibody Panels are community-validated resources that save time and money, enhance reproducibility, expedite discovery, and support the creation of a Human Reference Atlas.
Meeting Abstract
Immunology
Anna Pascual Reguant, Ralf Koehler, Ronja Mothes, Sandy Bauherr, Daniela Hernandez, Ralf Uecker, Karolin Holzwarth, Katja Kotsch, Maximilian Seidl, Lars Philipsen, Werner Muller, Chiara Romagnani, Raluca Niesner, Anja Hauser
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)